BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0178.0046.p (467 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309958|ref|YP_003774050.1| methyl-accepting chemotaxis tra... 583 e-167 gi|300310634|ref|YP_003774726.1| methyl-accepting chemotaxis dom... 324 1e-89 gi|300310428|ref|YP_003774520.1| methyl-accepting chemotaxis tra... 130 3e-31 gi|300311753|ref|YP_003775845.1| methyl-accepting chemotaxis tra... 127 1e-30 gi|300310789|ref|YP_003774881.1| methyl-accepting chemotaxis tra... 124 1e-29 >gi|300309958|ref|YP_003774050.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 595 Score = 583 bits (1502), Expect = e-167, Method: Compositional matrix adjust. Identities = 286/425 (67%), Positives = 342/425 (80%), Gaps = 5/425 (1%) Query: 1 MKNKLTQRMSIRQLFVWLVIVLAVLMGTIVSVTRALKEANTNLDRAHESRYYSFTLANEM 60 M+ +T RMSI+QLF+WL +VL++L+ +VS+T ALK+AN L+RAH+SRY S LANE+ Sbjct: 1 MRETMTHRMSIKQLFIWLALVLSILVAVVVSLTGALKDANLELERAHQSRYDSTALANEL 60 Query: 61 RRSSEDLTKFGRAYVTTGDPNDEERYYIVLDIRNGKRPRPKNYERVNLDLVMAKQLNAMP 120 RR++ED+TKF RAYVTTGDPNDE+RYY+++DIRNGKR RP NY+R N DLV A++L P Sbjct: 61 RRTAEDMTKFARAYVTTGDPNDEDRYYMIMDIRNGKRARPSNYDRFNWDLVNARKLALDP 120 Query: 121 ADPAVPLIDLMKQAGFTAQELAKMQEAKANSDALTKTEITAFNAVKGQFDDGAGNFTVTG 180 P VPL +LMK+AGFT +ELAKMQEA D L K EITAF+AVKG+FDDG G +TV G Sbjct: 121 GAPGVPLGELMKRAGFTDEELAKMQEALKLGDQLAKIEITAFHAVKGEFDDGEGKYTVIG 180 Query: 181 APNQAMAQELMFDQNYRTMVGRIMVPINDFLRMVDERTQRRVSVAQSRYSELEMVVFSLL 240 APNQAMAQEL+FDQ YRT+ G++M P+NDFLR+VDERTQ V A Y+ELE +F LL Sbjct: 181 APNQAMAQELVFDQTYRTLFGKLMSPVNDFLRLVDERTQANVRQAGKDYAELEQKIFWLL 240 Query: 241 GASMLLLLVCLFFTYRVIRSQLGGEPRDAMIVLRKVAEGDLTVQVPVSDRDTGSVLHSTQ 300 AS+++L CL+ +YR+IRSQ+GGEPRDAM VLR+VAEGDLTV VP+ D SVL+STQ Sbjct: 241 AASVVVLFACLWLSYRIIRSQIGGEPRDAMSVLRRVAEGDLTVVVPLPKGDKLSVLYSTQ 300 Query: 301 QMIAKWTAVIGDVNGTART-----PRSRLPTXALSKNASQQASNVEETSASVEEITATIA 355 QMI KWT VIGDV+GTA + + + ALS NA+QQA+NVEETSASVEEITATIA Sbjct: 301 QMINKWTDVIGDVSGTATSLASASEQISSSSQALSHNAAQQAANVEETSASVEEITATIA 360 Query: 356 QNAENARVTDSIASKSASSAVEGGEVVRETVLAMKQIAGKIGIIDDIAYQTNLLALNAAI 415 QNA+NAR TD IAS SAS+A EGGE VRETV AMKQIA KIGIIDDIAYQTNLLALNAAI Sbjct: 361 QNADNARTTDGIASLSASAATEGGEAVRETVAAMKQIASKIGIIDDIAYQTNLLALNAAI 420 Query: 416 EAAXA 420 EAA A Sbjct: 421 EAARA 425 >gi|300310634|ref|YP_003774726.1| methyl-accepting chemotaxis domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 568 Score = 324 bits (830), Expect = 1e-89, Method: Compositional matrix adjust. Identities = 186/439 (42%), Positives = 266/439 (60%), Gaps = 18/439 (4%) Query: 1 MKNKLTQRMSIRQLFVWLVIVLAVLMGTIVSVTRALKEANTNLDRAHESRYYSFTLANEM 60 MK L R SIRQ+F+ L V+ VLM IV A+ + L+ A+ESRY SF LA E+ Sbjct: 1 MKISLVSRFSIRQIFIMLTAVITVLMTLIVLAALAVDRYDARLNAANESRYASFLLATEL 60 Query: 61 RRSSEDLTKFGRAYVTTGDPNDEERYYIVLDIRNGKRPRPKNYERVNLDLVMAKQLNAMP 120 R+SS+DLT+ R YV TGDP+ E++Y +LDIRNGK+ RP++Y R+ D V A P Sbjct: 61 RQSSDDLTRLARTYVVTGDPSYEQQYEAILDIRNGKKARPEHYGRIYWDFVAADGKAPRP 120 Query: 121 -ADPAVPLIDLMKQAGFTAQELAKMQEAKANSDALTKTEITAFNAVKGQFDDGAGNFTVT 179 ++ V L DLM+ AGFTA E+ K++EAK NSDAL KTE+ A N VKG + D GNFT Sbjct: 121 DSNQHVALTDLMRAAGFTAAEMGKLEEAKNNSDALVKTEVIAMNMVKGLYADAQGNFTKR 180 Query: 180 GAPNQAMAQELMFDQNYRTMVGRIMVPINDFLRMVDERTQRRVSVAQSRYSELEMVVFSL 239 P+ A A+ELM + Y +IM P+++F ++D RT +V A+++ + + + +SL Sbjct: 181 ADPDLAQARELMHNHAYHVYKAKIMRPVDEFFALLDARTTEQVESARAQATHMATLFYSL 240 Query: 240 LGASMLLLLVCLFFTYRVIRSQLGGEPRDAMIVLRKVAEGDLTVQV-PVSDRDTGSVLHS 298 + ++++L L Y IR+ L A V R+VA GDLT Q+ P +TG +L + Sbjct: 241 IAIALVVLACTLMLVYHAIRNPLA----HAASVARRVAHGDLTAQIDPAIKGETGELLEA 296 Query: 299 TQQMIAKWTAVIGDVNGTAR---TPRSRLP--TXALSKNASQQASNVEETSASVEEITAT 353 M + ++ DV A+ T + + T LS QQAS++EET++S+EE+T+T Sbjct: 297 LTIMQSGLQTIVLDVRNGAQYINTSANEIASGTLDLSSRTEQQASSLEETASSMEELTST 356 Query: 354 IAQNAENARVTDSIASKSASSAVEGGEVVRETVLAM-------KQIAGKIGIIDDIAYQT 406 + QNA NAR+ +A+ ++ AV GGEVV V M K+I I +ID IA+QT Sbjct: 357 VKQNAGNARLASELANTASGVAVRGGEVVARVVDTMGAINESSKRIVDIIAVIDGIAFQT 416 Query: 407 NLLALNAAIEAAXARRRTR 425 N+LALNAA+EAA A + R Sbjct: 417 NILALNAAVEAARAGEQGR 435 >gi|300310428|ref|YP_003774520.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 517 Score = 130 bits (326), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 77/194 (39%), Positives = 121/194 (62%), Gaps = 6/194 (3%) Query: 232 LEMVVFSLLGASMLLLLVCLFFTYRVIRSQLGGEPRDAMIVLRKVAEGDLTVQVPVSDRD 291 LE V + G +L+LL + R + Q+GGEP D + V++++++GDL + + Sbjct: 193 LETVAL-VAGGVLLILLGAGWLLTRTLLRQMGGEPGDVVTVMQRLSDGDLVQTITLKPGC 251 Query: 292 TGSVLHSTQQMIAKWTAVIGDVNGTA-----RTPRSRLPTXALSKNASQQASNVEETSAS 346 S++H+ Q+M+ + V+G+V G A +LS+ +++QA+ VE+TS + Sbjct: 252 RDSMMHAIQKMMTRLAQVVGEVRGNADGLVSTAEEVSATAQSLSRASTEQANGVEQTSEA 311 Query: 347 VEEITATIAQNAENARVTDSIASKSASSAVEGGEVVRETVLAMKQIAGKIGIIDDIAYQT 406 + +I A + A+NAR+TD IA+ +A A + E V++ V A++QIAGKI +IDDIAYQT Sbjct: 312 LAQIHALLQSTAQNARLTDQIAAAAALEARQCAETVKQMVAAIQQIAGKIAVIDDIAYQT 371 Query: 407 NLLALNAAIEAAXA 420 NLLALNA IEAA A Sbjct: 372 NLLALNATIEAAHA 385 >gi|300311753|ref|YP_003775845.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 559 Score = 127 bits (320), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 121/402 (30%), Positives = 195/402 (48%), Gaps = 48/402 (11%) Query: 37 KEANTNLDRAHESRYYSFTLANEMRRSSEDLTKFGRAYVTTGDPNDEERYYIVLDIRNGK 96 + A L +A E+R+ + LA E R+ +DL + R+YV +G+P E++ VLDIR+G+ Sbjct: 35 ERARQALAQAQETRFTALALAAEFRQGVDDLAQLARSYVVSGEPRLLEQHQAVLDIRDGQ 94 Query: 97 RPRPKNYERVNLDLVMAKQLNAMPADP----AVPLIDLMKQAGFTAQELAKMQEAKANSD 152 R RP+ Y RV DLV A + P P AV L LM+QAG++ ELA++ EA SD Sbjct: 95 RARPQQYGRVYWDLVGA---DGQPPRPDSTVAVSLRKLMEQAGYSPAELARLDEAWLMSD 151 Query: 153 ALTKTEITAFNAVKGQFDDGAGNFTVTGAPNQAMAQELMFDQNYRTMVGRIMVPINDFLR 212 AL TE A ++G D GA + AQ+++ Y+ I+ +D + Sbjct: 152 ALASTERRAMQEMQG--DAGA----------RGPAQQMLHGDGYQLHRTSILQAFDDMVG 199 Query: 213 MVDERTQRRVSVAQSRYSELEMVVFSLLGASMLLLLVCLFFTYRVIRSQLGGEPRDAMIV 272 ++++RT R V++AQ L + L A +++L V L YR G A + Sbjct: 200 LLEQRTAREVALAQLLAQRLAWLSTLLAVAVVVILAVSLRLLYRGS----AGPLAQATSM 255 Query: 273 LRKVAEGDLTVQVPVSDRDTGSVLHSTQQMIAKWTAVIGDVNGT---------------A 317 L ++A GDL+ PV R + L Q + K A++ V G A Sbjct: 256 LAQLARGDLSDSAPVRARGQVAALLDALQRLRK--AIVRLVKGVHAGAAAIQNAAGDMAA 313 Query: 318 RTPRSRLPTXALSKNASQQASNVEETSASVEEITATIAQNAENARVTDSIASKSASSAVE 377 T T ++ Q A+++++ + +V++ TA Q + AR + +SA+ Sbjct: 314 GTEHVVARTEEQAQALLQAAASMQQLAGTVQQHTAHTHQASRLARRACEVVRRSAAEV-- 371 Query: 378 GGEVVRETVLAMKQIAGKI----GIIDDIAYQTNLLALNAAI 415 E V + A+K + +I G+ID IA Q+ LL +A++ Sbjct: 372 --EQVASAMAAIKASSARITDLGGLIDSIAVQSKLLGASASL 411 >gi|300310789|ref|YP_003774881.1| methyl-accepting chemotaxis transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 542 Score = 124 bits (312), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 78/247 (31%), Positives = 135/247 (54%), Gaps = 12/247 (4%) Query: 191 MFDQNYRTMVGRIMVPINDFLRMVDERTQRRVSVAQSRYSELEMVVFSLLGASMLLLLVC 250 + Q R + + + +++ R DE + + A + Y + ++L+ S+LL + Sbjct: 148 LLTQKIRPVQAKWLDNLDELGRFEDELNNQASAAADTTYRSILTTTWTLVAGSVLLAVAA 207 Query: 251 LFFTYRVIRSQLGGEPRDAMIVLRKVAEGDLTVQVPVSDRDTGSVLHSTQQMIAKWTAVI 310 F R + QLGGEP A + ++A+G+L V +P++ D S++ S + M A+ T ++ Sbjct: 208 AFLIVRSVLHQLGGEPAQAQELAAQIAQGNLAVSIPLAKGDQHSLMASLENMRAQLTRIV 267 Query: 311 GDVNGTARTPRSRLPTXA-----LSKNASQQASNVEETSASVEEITATIAQNAENARVTD 365 + +A + + A LS+ +QA+++EET++S+EEIT+T+ QN +NA Sbjct: 268 DGIKHSAESISTAASQIAVGNLDLSRRTEEQAASLEETASSMEEITSTVEQNTQNAGQGH 327 Query: 366 SIASKSASSAVEGGEVVRETVLAMKQIAGK-------IGIIDDIAYQTNLLALNAAIEAA 418 +A ++ A +GGEVV V MK I+ I ++ IA+QTN+LALNAA+EAA Sbjct: 328 QLARSASLHAQKGGEVVERVVGTMKDISQSAYKITEIIATVEGIAFQTNILALNAAVEAA 387 Query: 419 XARRRTR 425 A + R Sbjct: 388 RAGEQGR 394 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.130 0.357 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,089,202 Number of extensions: 37915 Number of successful extensions: 293 Number of sequences better than 1.0e-08: 64 Number of HSP's gapped: 230 Number of HSP's successfully gapped: 64 Length of query: 467 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 378 Effective length of database: 1,198,455 Effective search space: 453015990 Effective search space used: 453015990 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)