BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0178.0044.p (472 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309957|ref|YP_003774049.1| chemotaxis histidine kinase [He... 389 e-109 gi|300311033|ref|YP_003775125.1| chemotaxis histidine kinase [He... 63 5e-11 gi|300312396|ref|YP_003776488.1| chemotaxis CheA protein [Herbas... 59 7e-10 gi|300312286|ref|YP_003776378.1| signal-transducing histidine ki... 59 1e-09 >gi|300309957|ref|YP_003774049.1| chemotaxis histidine kinase [Herbaspirillum seropedicae SmR1] Length = 725 Score = 389 bits (999), Expect = e-109, Method: Compositional matrix adjust. Identities = 224/417 (53%), Positives = 279/417 (66%), Gaps = 32/417 (7%) Query: 1 MKKDAARDALVQEARELLVAMEAALLQIESDGASRDAINAIFRAAHTIKGSAGLFAFDSI 60 M KD+AR A+VQEARELLVAMEAALLQ E +G SRD+I+AIFRAAHTIKGSAGLFAFDSI Sbjct: 1 MSKDSARHAMVQEARELLVAMEAALLQTEMEGPSRDSIHAIFRAAHTIKGSAGLFAFDSI 60 Query: 61 VSFTHLLENVLDKVRNGVVKLDDAMMSLLLNCGDYMNELVDAIAELKEEQDPDPARGVPT 120 V FTH LE+VL++VR + LD +MSLLL C DY+ ELVDAI +E ++P+ R Sbjct: 61 VQFTHHLEHVLERVREERLALDSHLMSLLLQCSDYIGELVDAIERGQEAEEPNAERRAAL 120 Query: 121 L---------------RSSTLPLSSAPAGAGGAVET-DDGAVANPYWHLSLRFSPDVLRD 164 L S +PL++ AG T D A PYWHLSL+ + +VLRD Sbjct: 121 LAELDAVAAFSVDHGPASVPVPLAALRGAAGSESATADQAATRVPYWHLSLQLNENVLRD 180 Query: 165 GLDPLSFLRYLLTMGSMVYVHVIDLAIPPADDMDAELCYLGFEIGFASTSRREEIDSVFE 224 GLDPLSFL YL ++G +V + ++ AIP A MDAE CYLG E+ AS S + +++VFE Sbjct: 181 GLDPLSFLHYLRSLGRIVALVTVESAIPEAAAMDAESCYLGMEVCLASDSTLQTLENVFE 240 Query: 225 FVREDSDIVILAPHSQLAEYRDWLQHLPEREEIALHLLQSGALTAAEWR------QLQFD 278 FVR+DS I IL P + L+ Y + L L Q A AA WR Q Q+ Sbjct: 241 FVRDDSRIDILPPRAPLSAYAERLARLAP---------QDAARLAAAWRGLALFDQTQWQ 291 Query: 279 AIDAADAVADAGMHAEASSQVSAPMPAMRQKAGEEKKSLEQKFIKIEVSKLDQLIDLVGE 338 +++AA A + A A ++A R +A +E+++ EQKFIKIEVSKLDQLIDLVGE Sbjct: 292 SLEAASATPSDAL-AAAVEPMAAEGVLQRPRALDERRAQEQKFIKIEVSKLDQLIDLVGE 350 Query: 339 LVIAGAGASLVAKRKKDQRFEEATQVISGLVEQIRDAALTLRMVQIKEVFQRYPRVV 395 LVIAGA A L+A+R+KD +FEEATQ I L+EQIRDAALTLRMVQI EVFQR+PR+V Sbjct: 351 LVIAGASARLIARRRKDSQFEEATQAIDTLMEQIRDAALTLRMVQINEVFQRFPRIV 407 >gi|300311033|ref|YP_003775125.1| chemotaxis histidine kinase [Herbaspirillum seropedicae SmR1] Length = 837 Score = 62.8 bits (151), Expect = 5e-11, Method: Compositional matrix adjust. Identities = 40/133 (30%), Positives = 64/133 (48%), Gaps = 1/133 (0%) Query: 7 RDALVQEARELLVAMEAALLQIESDGASRDAINAIFRAAHTIKGSAGLFAFDSIVSFTHL 66 RD + E R+LL + L+Q+E + + ++FR HT+KG++GLF + H Sbjct: 5 RDQFILETRDLLQGISEKLMQLEDAPEHVELLESLFRMVHTLKGNSGLFDVPEMTRVLHA 64 Query: 67 LENVLDKVRNGVVKLDDAMMSLLLNCGDYMNELVDAIAELKEEQDPDPARGVPTLRSSTL 126 E+++D VR V + + LL+ D++ L D + E+ DP V + SS Sbjct: 65 CEDLMDAVRRRQVAYSEVLADRLLDAMDFVAMLCDGLEMEAEQADPHAQEAV-QIASSLR 123 Query: 127 PLSSAPAGAGGAV 139 L AG AV Sbjct: 124 QLIGEHAGVESAV 136 Score = 57.0 bits (136), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 28/85 (32%), Positives = 53/85 (62%), Gaps = 8/85 (9%) Query: 319 QKFIKIEVSKLDQLIDLVGELVIAGAGASLVAKRKKDQ--------RFEEATQVISGLVE 370 K +K+E K+D+L++L+GE+V++ +A+R +DQ + VI+ + E Sbjct: 448 NKSVKVEQVKIDRLMNLIGEMVVSKNALPYLAQRAEDQFGVRELAREIKAQYAVINRIAE 507 Query: 371 QIRDAALTLRMVQIKEVFQRYPRVV 395 +++DA + +RM+ + VFQR+PR+V Sbjct: 508 EMQDAIMQVRMMPVSVVFQRFPRLV 532 >gi|300312396|ref|YP_003776488.1| chemotaxis CheA protein [Herbaspirillum seropedicae SmR1] Length = 638 Score = 58.9 bits (141), Expect = 7e-10, Method: Compositional matrix adjust. Identities = 37/110 (33%), Positives = 52/110 (47%), Gaps = 3/110 (2%) Query: 10 LVQEARELLVAMEAALLQIESDGASRDAINAIFRAAHTIKGSAGLFAFDSIVSFTHLLEN 69 V EA EL +E LL +E + +NA+FRA HTIKG AG ++VS HL EN Sbjct: 9 FVVEALELATNVEEHLLTLERHPDDLEILNAVFRAFHTIKGGAGFVNLPAMVSACHLTEN 68 Query: 70 VLDKVRNGVVKLDDAMMSLLLNCGDYMNELVDAIAELKEEQDPDPARGVP 119 + D +R G + + L + + D + EL PD +P Sbjct: 69 LFDALRTGKAPVTPLAIEAALQASGF---VADQLNELSNGAAPDSLASMP 115 >gi|300312286|ref|YP_003776378.1| signal-transducing histidine kinase CheA protein [Herbaspirillum seropedicae SmR1] Length = 697 Score = 58.5 bits (140), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 28/82 (34%), Positives = 44/82 (53%) Query: 21 MEAALLQIESDGASRDAINAIFRAAHTIKGSAGLFAFDSIVSFTHLLENVLDKVRNGVVK 80 ME LL + + +NA+FRAAH+IKG + F I TH+LE++LD++R G + Sbjct: 1 MEKLLLAVNVASPDPEDLNAVFRAAHSIKGGSSTFGLTDIAEVTHVLESLLDRIRQGQMA 60 Query: 81 LDDAMMSLLLNCGDYMNELVDA 102 L + L D + ++D Sbjct: 61 LTSEHVDAFLAAKDVLKSMLDG 82 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,087,692 Number of extensions: 38272 Number of successful extensions: 159 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 155 Number of HSP's successfully gapped: 5 Length of query: 472 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 383 Effective length of database: 1,198,455 Effective search space: 459008265 Effective search space used: 459008265 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)