BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0178.0038.p (120 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309952|ref|YP_003774044.1| 3-methyl-2-oxobutanoate hydroxy... 157 2e-40 >gi|300309952|ref|YP_003774044.1| 3-methyl-2-oxobutanoate hydroxymethyltransferase [Herbaspirillum seropedicae SmR1] Length = 275 Score = 157 bits (397), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 75/106 (70%), Positives = 88/106 (83%) Query: 1 MAAYLQDAPATRKKAVTIHTLQTMRDKGDKITMLTCYDASFAALMDRSGVDTVLIGDSLG 60 MA YLQ++ A R K VT HTLQ MR KGDKI+MLTCYDASFA LM+R+G+DTVLIGDSLG Sbjct: 1 MAGYLQESGAARAKPVTTHTLQAMRAKGDKISMLTCYDASFATLMERAGLDTVLIGDSLG 60 Query: 61 MVCQGHQSTLPVTVADIAYHCASVARGNQSMLIIADMPGGGPGVRQ 106 MVCQGH STLPVT+ D+AYH A+VARG ++MLIIAD+P G R+ Sbjct: 61 MVCQGHDSTLPVTIQDVAYHTAAVARGTRTMLIIADLPFGTYPTRE 106 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.133 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 392,057 Number of extensions: 14463 Number of successful extensions: 32 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 32 Number of HSP's successfully gapped: 1 Length of query: 120 Length of database: 1,619,870 Length adjustment: 74 Effective length of query: 46 Effective length of database: 1,269,480 Effective search space: 58396080 Effective search space used: 58396080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 125 (52.8 bits)