BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0178.0034.p (646 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309950|ref|YP_003774042.1| molecular chaperone protein [He... 1184 0.0 gi|300312439|ref|YP_003776531.1| molecular chaperone protein [He... 451 e-127 gi|300312141|ref|YP_003776233.1| molecular chaperone, Hsp70 fami... 438 e-124 >gi|300309950|ref|YP_003774042.1| molecular chaperone protein [Herbaspirillum seropedicae SmR1] Length = 646 Score = 1184 bits (3063), Expect = 0.0, Method: Compositional matrix adjust. Identities = 608/646 (94%), Positives = 625/646 (96%) Query: 1 MGKIIGIDLGTTNSCVSVMEGGQPKVIENSEGARTTPSIIAYQEDGEVLVGAPAKRQAVT 60 MGKIIGIDLGTTNSCV+VMEG QPKVIENSEGARTTPSIIAYQEDGE+LVGAPAKRQAVT Sbjct: 1 MGKIIGIDLGTTNSCVAVMEGNQPKVIENSEGARTTPSIIAYQEDGEILVGAPAKRQAVT 60 Query: 61 NPKNTIYAAKRLIGRKFDEKEVQKDISLMPYAIIKADNGDAWIGVRDNKLAPPQISAEVL 120 NPKNTIYAAKRLIGRKF EKEVQKDI+LMPY I+ ADNGDAWIGVRD KLAPPQISAEVL Sbjct: 61 NPKNTIYAAKRLIGRKFTEKEVQKDINLMPYQIVAADNGDAWIGVRDKKLAPPQISAEVL 120 Query: 121 RKMKKTAEDYLGEEVTEAVITVPAYFNDAQRQATKDAGRIAGLEVKRIINEPTAAALAFG 180 RKMKKTAEDYLGEEVTEAVITVPAYFNDAQRQATKDAGRIAGL+VKRIINEPTAAALAFG Sbjct: 121 RKMKKTAEDYLGEEVTEAVITVPAYFNDAQRQATKDAGRIAGLDVKRIINEPTAAALAFG 180 Query: 181 LDKTEKGDRKIAVYDLGGGTFDVSIIEIADVDGEKQFEVLSTNGDTFLGGEDFDQRIIDY 240 LDK EKGDRKIAVYDLGGGTFDVSIIEIADVDGEKQFEVLSTNGDTFLGGEDFDQR+IDY Sbjct: 181 LDKAEKGDRKIAVYDLGGGTFDVSIIEIADVDGEKQFEVLSTNGDTFLGGEDFDQRLIDY 240 Query: 241 IIDEFKKINGLDLSKDAIALQRIKASAERAKIELSSSQQTEINEPYIAMANGAPVHLNLK 300 IIDEFKKINGLDLSKDAIALQRIKASAERAKIELSSSQQTEINEPYIAMANGAPVHLNLK Sbjct: 241 IIDEFKKINGLDLSKDAIALQRIKASAERAKIELSSSQQTEINEPYIAMANGAPVHLNLK 300 Query: 301 ITRAKLESLVEELIAKTIEPCRTAIKDAGVKVSDIDDIILVGGMTRMPKVQEKVKEFFGK 360 ITRAKLESLVEELIAKTIEPCRTAIKDAGVKVSDIDD+ILVGGMTRMPKV EKVKEFFGK Sbjct: 301 ITRAKLESLVEELIAKTIEPCRTAIKDAGVKVSDIDDVILVGGMTRMPKVIEKVKEFFGK 360 Query: 361 DPRKDVNPDEAVAVGAAIQGSVLSGERKDLLLLDVTPLSLGIETMGGVMTKMIKKNTTIP 420 DPR+DVNPDEAVAVGAAIQGSVLSG+RKD+LLLDVTPLSLGIETMGGVMTKMIKKNTTIP Sbjct: 361 DPRRDVNPDEAVAVGAAIQGSVLSGDRKDVLLLDVTPLSLGIETMGGVMTKMIKKNTTIP 420 Query: 421 TKFSQVFSTADDNQPAVTIKVYQGEREMAAGNKALGEFNLEGIPPSPRGTPQIEVTFDID 480 TKFSQVFSTADDNQPAVTIKVYQGEREMA GNKALGEFNLEGIPP+PRGTPQIEVTFDID Sbjct: 421 TKFSQVFSTADDNQPAVTIKVYQGEREMAVGNKALGEFNLEGIPPAPRGTPQIEVTFDID 480 Query: 481 ANGILHVGAKDKATGKENKVTIKANSGLNEEEIEKMVRDAEANADEDKRLKELAETRNQG 540 ANGILHVGAKDKATGKENK+TIKANSGLNEEEIEKMVRDAEANA+EDKRLKELAETRNQG Sbjct: 481 ANGILHVGAKDKATGKENKITIKANSGLNEEEIEKMVRDAEANAEEDKRLKELAETRNQG 540 Query: 541 DALVHSTRKALTEYGDKLEAGEKDAIEAAIKELEEVLKANDKAAIDAKTAALSVAAQKLG 600 DALVHST+KAL EYGDKLEAGEKD IEAAIK LEE LK +DKAAIDAKT ALS AAQKLG Sbjct: 541 DALVHSTKKALGEYGDKLEAGEKDVIEAAIKNLEEALKGDDKAAIDAKTEALSTAAQKLG 600 Query: 601 EKMYADQQAQQAAAGGAAGAAGGSAGAGESRPKDDDVVDADFKEVK 646 EKMYADQQAQ AAAGGAAGAAG AGA + PKDD+VVDADFKEVK Sbjct: 601 EKMYADQQAQAAAAGGAAGAAGAQAGAAGAAPKDDNVVDADFKEVK 646 >gi|300312439|ref|YP_003776531.1| molecular chaperone protein [Herbaspirillum seropedicae SmR1] Length = 621 Score = 451 bits (1159), Expect = e-127, Method: Compositional matrix adjust. Identities = 254/617 (41%), Positives = 381/617 (61%), Gaps = 30/617 (4%) Query: 5 IGIDLGTTNSCVSVMEGGQPKVIENSEGARTTPSIIAYQEDGEVLVGAPAKRQAVTNPKN 64 +GIDLGTTNS V+ + P+V+ + +G PS++ Y +G +G A+ T+P+N Sbjct: 22 VGIDLGTTNSLVATVRNSIPEVLADDDGRVLLPSVVRYLPNGHANIGYKAQAAQTTDPRN 81 Query: 65 TIYAAKRLIGRKFDEKEVQKDISL---MPYAIIKADNGDAWIGVRDNKLAPPQISAEVLR 121 TI + KR +GR KDI+ MPY + G I +P ++SA++L Sbjct: 82 TIVSVKRFMGRGL------KDIAHAENMPYDF-QDQPGMVQIKTVAGVKSPVEVSAQILA 134 Query: 122 KMKKTAEDYLGEEVTEAVITVPAYFNDAQRQATKDAGRIAGLEVKRIINEPTAAALAFGL 181 +++TAED LG+++ AVITVPAYF+DAQRQATKDA ++AGL V R++NEPTAAA+A+GL Sbjct: 135 TLRQTAEDALGDDLVGAVITVPAYFDDAQRQATKDAAQLAGLNVLRLLNEPTAAAIAYGL 194 Query: 182 DKTEKGDRKIAVYDLGGGTFDVSIIEIADVDGEKQFEVLSTNGDTFLGGEDFDQRIIDYI 241 D + +G AVYDLGGGTFD+SI+++ FEVL+T GD+ LGG+DFD R+ +I Sbjct: 195 DNSSEG--VFAVYDLGGGTFDISILKLT----RGVFEVLATGGDSALGGDDFDHRLFCWI 248 Query: 242 IDEFKKINGLDLSKDAIALQRIKASAERAKIELSSSQQTEINEPYIAMANGAPVHLNLKI 301 E + LS + L +KA K LS+ + +I+ + +G VHL+ I Sbjct: 249 SHEAQL---QPLSDEDTRLLMVKA--REVKELLSTKDEVQIDA---VLRSGEEVHLS--I 298 Query: 302 TRAKLESLVEELIAKTIEPCRTAIKDAGVKVSDIDDIILVGGMTRMPKVQEKVKEFFGKD 361 T A+ + + L+AKT+ P R A++DAG+ V D+D ++LVGG TRMP ++ V EFF + Sbjct: 299 TAAQFNEITQHLVAKTMAPVRKALRDAGLTVEDVDGVVLVGGATRMPHIRRAVGEFFKTN 358 Query: 362 PRKDVNPDEAVAVGAAIQGSVLSGERK---DLLLLDVTPLSLGIETMGGVMTKMIKKNTT 418 P +++PD+ VA+GAA+Q ++L+G R D LLLDV PLSLGIETMGG+ K+I +N+T Sbjct: 359 PLSNIDPDKVVALGAAVQANLLAGNRAPGDDWLLLDVIPLSLGIETMGGLAEKVIPRNST 418 Query: 419 IPTKFSQVFSTADDNQPAVTIKVYQGEREMAAGNKALGEFNLEGIPPSPRGTPQIEVTFD 478 IP +Q F+T D Q A+ I V QGERE+ A ++L F L GIPP G +I VT+ Sbjct: 419 IPCARAQEFTTFKDGQTAMAIHVVQGERELVADCRSLARFELRGIPPMAAGAARIRVTYQ 478 Query: 479 IDANGILHVGAKDKATGKENKVTIKANSGLNEEEIEKMVRDAEANADEDKRLKELAETRN 538 +DA+G+L V A++ +G E +T+K + GL ++EI +M++++ ++ADED + + L E + Sbjct: 479 VDADGLLAVSAREMTSGVEASITVKPSYGLGDDEIARMLQESFSSADEDMQRRALREEQV 538 Query: 539 QGDALVHSTRKALTEYGDKLEAGEKDAIEAAIKELEEVLKANDKAAIDAKTAALSVAAQK 598 + + +V +TR AL L E+ AIEA ++ + + D AI A AL+ ++ Sbjct: 539 EAERIVLATRSALQADAALLSEAERQAIEAQVEAVSAAAQGTDHLAIKAAVDALAHGTEE 598 Query: 599 LGEKMYADQQAQQAAAG 615 + D+ A AG Sbjct: 599 FAARRM-DRSVHAALAG 614 >gi|300312141|ref|YP_003776233.1| molecular chaperone, Hsp70 family [Herbaspirillum seropedicae SmR1] Length = 642 Score = 438 bits (1126), Expect = e-124, Method: Compositional matrix adjust. Identities = 258/618 (41%), Positives = 385/618 (62%), Gaps = 39/618 (6%) Query: 5 IGIDLGTTNSCVSVMEGGQPKVIENSEGARTTPSIIAYQEDGEV--------LVGAPAKR 56 +GIDLGTTNS V+ ++ GQ +++ + G PS++ Y + +VG AK Sbjct: 22 VGIDLGTTNSLVATVQSGQARILPDEAGQALLPSVVRYLPAHSLQPADRPPAVVGHAAKA 81 Query: 57 QAVTNPKNTIYAAKRLIGRKFDEKEVQKDISL-MPYAIIKADNGDAWIGVRDNKLAPPQI 115 +P+NT+ + KRL+GR D ++ S MPYA I G I P + Sbjct: 82 AQSDDPRNTLSSIKRLMGR--DRADISAGNSADMPYAFID-KPGLVQIDTAAGIKTPIDV 138 Query: 116 SAEVLRKMKKTAEDYLGEEVTEAVITVPAYFNDAQRQATKDAGRIAGLEVKRIINEPTAA 175 SA++LR +++ AE LG E++ AVITVPAYF+DAQRQATKDA R+AGL V R++NEPTAA Sbjct: 139 SADILRVLRQRAEASLGGELSGAVITVPAYFDDAQRQATKDAARLAGLNVLRLLNEPTAA 198 Query: 176 ALAFGLDKTEKGDRKIAVYDLGGGTFDVSIIEIADVDGEKQFEVLSTNGDTFLGGEDFDQ 235 A+A+GLD+ +G A+YDLGGGTFD+SI++++ FEVL+TNGD LGG+DFD+ Sbjct: 199 AIAYGLDQAAEG--VFAIYDLGGGTFDISILKLS----RGVFEVLATNGDGALGGDDFDR 252 Query: 236 RIIDYIIDEFKKINGLDLSKDAIALQRIKASAERAKIELS--SSQQTEINEPYIAMANGA 293 I D+I+D+ + ++D +R+ +A +AK LS +S E+N +++G Sbjct: 253 CIHDWIVDQSPLASAELTTQDT---RRLLTAARQAKETLSRETSATIELN-----LSSGQ 304 Query: 294 PVHLNLKITRAKLESLVEELIAKTIEPCRTAIKDAGVKVSDIDDIILVGGMTRMPKVQEK 353 V L L TR S+ + L+A+T+ P R A++DAG+ VS+ID ++LVGG TRMP+V+ Sbjct: 305 AVQLAL--TREVFASISQGLLARTLLPTRKALRDAGLGVSEIDGVVLVGGSTRMPQVRAA 362 Query: 354 VKEFFGKDPRKDVNPDEAVAVGAAIQGSVLSGERK--DLLLLDVTPLSLGIETMGGVMTK 411 V +FFG+ P D++PD+ VA+GAA+Q +VL+G R+ D LLLDV PLSLG+ETMGG++ K Sbjct: 363 VADFFGQPPLTDLDPDQVVALGAAMQANVLAGNRQQSDWLLLDVCPLSLGLETMGGLVEK 422 Query: 412 MIKKNTTIPTKFSQVFSTADDNQPAVTIKVYQGEREMAAGNKALGEFNLEGIPPSPRGTP 471 +I +N+TIP +Q F+T D Q A+++ V QGERE+ A ++L L GIPP G Sbjct: 423 IIPRNSTIPVARAQEFTTFKDGQTAMSVHVLQGERELVADCRSLARLELRGIPPMVAGAA 482 Query: 472 QIEVTFDIDANGILHVGAKDKATGKENKVTIKANSGLNEEEIEKMVRDAEANADEDKRLK 531 +I VTF IDA+G+L V A++++TG E VT+K + GL++ I +M+ +A +A +D +++ Sbjct: 483 RIRVTFQIDADGLLSVSAREQSTGVEAAVTVKPSYGLDDANIARMLSEAVGHATDDMKMR 542 Query: 532 ELAETRNQGDALVHSTRKALTEYGDK-LEAGEKDAIEAAIKELEEVL----KANDK--AA 584 L E + + +T AL G L A E+ I + L E++ +AND AA Sbjct: 543 MLREAQVNARQVADATDAALKRDGHSLLSATEQAHIRQHLYALTELIETAREANDDNIAA 602 Query: 585 IDAKTAALSVAAQKLGEK 602 + AL+ A Q+ + Sbjct: 603 LGRACDALNQATQEFAAR 620 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.311 0.131 0.353 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,795,140 Number of extensions: 73695 Number of successful extensions: 226 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 214 Number of HSP's successfully gapped: 4 Length of query: 646 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 555 Effective length of database: 1,188,985 Effective search space: 659886675 Effective search space used: 659886675 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 134 (56.2 bits)