BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0178.0027.p (170 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309945|ref|YP_003774037.1| phosphatase (HAD superfamily) [... 157 3e-40 >gi|300309945|ref|YP_003774037.1| phosphatase (HAD superfamily) [Herbaspirillum seropedicae SmR1] Length = 244 Score = 157 bits (398), Expect = 3e-40, Method: Compositional matrix adjust. Identities = 85/149 (57%), Positives = 97/149 (65%), Gaps = 16/149 (10%) Query: 4 TNIQAVLFDLDDTLWAIEPVLHLAETRLYEWLTRHAPGVVQRESIISLRARAVGNAKASS 63 + IQAVLFDLDDTLWAIEPVL AET LY+WL +HAP V +I SLR R Sbjct: 15 SPIQAVLFDLDDTLWAIEPVLVRAETLLYDWLRQHAPAVAAAHTIASLRER--------- 65 Query: 64 ACARAARRQALAKTDPVYEINLWALRHAALTAVLAEHGEDPALADPAMEIFTAGRNAVTL 123 R AL +TDP Y INLW LRH AL+ V EH D AL DPAM +F+ R+ V L Sbjct: 66 -------RMALMQTDPAYRINLWKLRHTALSEVFREHDVDLALVDPAMALFSEARSTVAL 118 Query: 124 FDDVLPGLQQLKPRLKLGTVSNGFADLAR 152 FDDV P L +LK +L LG+VSNGFADL R Sbjct: 119 FDDVEPALLRLKGKLTLGSVSNGFADLER 147 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.133 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 478,671 Number of extensions: 15796 Number of successful extensions: 69 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 68 Number of HSP's successfully gapped: 1 Length of query: 170 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 91 Effective length of database: 1,245,805 Effective search space: 113368255 Effective search space used: 113368255 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 127 (53.5 bits)