BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0170.0009.p (816 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313465|ref|YP_003777557.1| penicillin-binding 1 (peptidogl... 1390 0.0 gi|300310721|ref|YP_003774813.1| penicillin-binding 1 (peptidogl... 857 0.0 gi|300312242|ref|YP_003776334.1| penicillin-binding 2 protein [H... 97 4e-21 gi|300309743|ref|YP_003773835.1| monofunctional biosynthetic pep... 78 3e-15 gi|300309672|ref|YP_003773764.1| cell division FtsI protein/peni... 58 4e-09 >gi|300313465|ref|YP_003777557.1| penicillin-binding 1 (peptidoglycan synthetase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 771 Score = 1390 bits (3598), Expect = 0.0, Method: Compositional matrix adjust. Identities = 661/772 (85%), Positives = 719/772 (93%), Gaps = 2/772 (0%) Query: 46 LHPVLRVLLGGIGVLVALVLMAVLVLGFVVTLAYPKLPDLDTLTDYRPKIPLRVFSADGV 105 +H LR+LLG +G + L +M +L + F+V L YPKLPDLDTLTDYRPKIPLR+FSADGV Sbjct: 1 MHWSLRILLGLVGTIFGLAVMVLLGVTFIVALTYPKLPDLDTLTDYRPKIPLRIFSADGV 60 Query: 106 LIGEFGEERRNLVHIKDIPDIMKKAVLAIEDDRFYEHGGVDYQGIVRASFANLSGGGGGA 165 LIGEFGEERRNLVH KDIP+IMKKAVLAIEDDRFYEHGGVDYQGI RA+F+NLSGGGGGA Sbjct: 61 LIGEFGEERRNLVHFKDIPEIMKKAVLAIEDDRFYEHGGVDYQGIARATFSNLSGGGGGA 120 Query: 166 STITQQVARNFFLTSNEPTFLQKASRKFLYEIPLAWKIERNLTKDQILEVYMNQIYLGQR 225 STITQQVARNFFLTSNEPTFLQKA+RKF YEIPLAWKIE NLTKDQILEVYMNQIYLGQR Sbjct: 121 STITQQVARNFFLTSNEPTFLQKATRKFFYEIPLAWKIENNLTKDQILEVYMNQIYLGQR 180 Query: 226 AYGFSSAAQIYFGKQLKELTIAESAMLAGLPKAPSAYNPVVNPKRAHARQQYILLRMRQL 285 AYGF+SAAQIYFGKQLK+LTIAE+AMLAGLPKAPSAYNPVVNPKRAHARQQYILLRMRQL Sbjct: 181 AYGFASAAQIYFGKQLKDLTIAEAAMLAGLPKAPSAYNPVVNPKRAHARQQYILLRMRQL 240 Query: 286 GYITDQQYTQAKDEELKVKTDSTAFGVHAEYVSEMARQLVYAQFKDDTYTRGLNVFTTIT 345 GYITD QY AKDEEL+VK DS+AFG+HAEYV+EMARQLVYAQ+KD+TYTRGLNVFTTIT Sbjct: 241 GYITDAQYQAAKDEELRVKGDSSAFGMHAEYVAEMARQLVYAQYKDETYTRGLNVFTTIT 300 Query: 346 KADQDAAYLALRRGIIDYERRHGYRGPEGYMEIP-TASKEEADDAIESELADHPDSDDLI 404 K DQDAAY+ALRRGIIDYERRHGYRGPEGYM++P A+K+ +DA+E ELADHPDSDD++ Sbjct: 301 KTDQDAAYIALRRGIIDYERRHGYRGPEGYMDLPENATKDAVEDAVEVELADHPDSDDMV 360 Query: 405 AAVVLEATTKEITAVLATGDEIKISGSGLGFAGNLLSEKAPPQKKIKRGAIIRVFQDGKN 464 +AVVLEA KEITA+L+TG+EI ISG+GLGF NLLS+KAPPQKKI+RGAIIRVFQDG N Sbjct: 361 SAVVLEAKPKEITAMLSTGEEISISGAGLGFGANLLSDKAPPQKKIRRGAIIRVFQDGNN 420 Query: 465 WIVTQMPEVESAFISLDPNDGSIRSLVGGFDFNRNKFNHVIQAWRQPGSSFKPFIYSSSL 524 WI+TQMPEVESAF+S++ DG+IRSLVGGFDFNRNKFNHV QAWRQPGSSFKPFIYSSSL Sbjct: 421 WIITQMPEVESAFVSVNSQDGAIRSLVGGFDFNRNKFNHVTQAWRQPGSSFKPFIYSSSL 480 Query: 525 EKGLSPATIINDAPLTFTQTGGQVWQPKNYGGKFEGPISMRRALQKSINLVSIRILERVG 584 EKGLSPATIINDAPLTFTQTGGQVWQPKNYGGKFEGPISMRRALQKSINLVSIRILERVG Sbjct: 481 EKGLSPATIINDAPLTFTQTGGQVWQPKNYGGKFEGPISMRRALQKSINLVSIRILERVG 540 Query: 585 VKYAQEYITRFGFDADKNPPYLTLALGAGAVTPLQMVGAYSVFANGGYKINPYLISKITD 644 VKYAQEYITRFGFDADKNPPYLTLALGAG VTPLQMVGAYSVFANGGYKINPYLI+K+TD Sbjct: 541 VKYAQEYITRFGFDADKNPPYLTLALGAGNVTPLQMVGAYSVFANGGYKINPYLITKVTD 600 Query: 645 NNGNTLSEAQPARSGDEANRVIDARNAFVMDSMLRDVVRHGTAVKALSLKRTDLAGKTGT 704 +NG LS+A+PA + DE+NRVIDARNAF+MDSMLRDVVRHGTAVKALSLKRTDLAGKTGT Sbjct: 601 SNGEVLSQAKPATADDESNRVIDARNAFIMDSMLRDVVRHGTAVKALSLKRTDLAGKTGT 660 Query: 705 TNDSMDAWFAGYQPNLVGIAWMGYDQPKSLGDRETGGGLALPIWISYMQKALKDVPMVDR 764 TNDSMDAWFAGYQP L IAW+GYDQPKSLGDRETGGGLALP+WISYM KALKDVP VDR Sbjct: 661 TNDSMDAWFAGYQPGLTAIAWIGYDQPKSLGDRETGGGLALPVWISYMSKALKDVPNVDR 720 Query: 765 TVPSGLIESGGEYYYAEYPPSASVRDLGLGEHGAAASPEEEKAKNQMKNELF 816 VP GLIESGGEYYYAE+PPSA VRDLG+G+ + +PEEEKAK QMKNELF Sbjct: 721 KVPDGLIESGGEYYYAEFPPSAMVRDLGMGDR-SGLTPEEEKAKTQMKNELF 771 >gi|300310721|ref|YP_003774813.1| penicillin-binding 1 (peptidoglycan synthetase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 822 Score = 857 bits (2214), Expect = 0.0, Method: Compositional matrix adjust. Identities = 443/761 (58%), Positives = 567/761 (74%), Gaps = 19/761 (2%) Query: 32 PGSGSAPVKKARKRLHPVLRVLLGG--IGVLVALVLMAVLVLGFVVTLAYPKLPDLDTLT 89 P S P+ + R L+P + +G +G+ VA L+ V L F+ P+LP L+ +T Sbjct: 7 PRPHSRPLTRQRGFLNPNIAKFIGLSILGLGVAGALLVVYALIFI----NPRLPSLELIT 62 Query: 90 DYRPKIPLRVFSADGVLIGEFGEERRNLVHIKDIPDIMKKAVLAIEDDRFYEHGGVDYQG 149 DYRPK+PLR++S D VL+GEFGEE+R+LV + DIP MK AVLAIED RFYEHGG+D+ G Sbjct: 63 DYRPKVPLRIWSEDKVLLGEFGEEKRSLVKLDDIPADMKNAVLAIEDYRFYEHGGIDWIG 122 Query: 150 IVRASFANLSGGGG--GASTITQQVARNFFLTSNEPTFLQKASRKFLYEIPLAWKIERNL 207 I RA ++ G GASTIT QVARN FLT E TF SRK LYE+ LA KIE L Sbjct: 123 IARALVTDVLRGSASQGASTITMQVARNVFLTK-EKTF----SRK-LYEVLLANKIEHAL 176 Query: 208 TKDQILEVYMNQIYLGQRAYGFSSAAQIYFGKQLKELTIAESAMLAGLPKAPSAYNPVVN 267 TKDQILEVYMNQIYLGQRA+GF++AA+ YFGK LK++T+AE+AMLAGLPKAPSAYNP+VN Sbjct: 177 TKDQILEVYMNQIYLGQRAFGFAAAARTYFGKDLKDVTLAEAAMLAGLPKAPSAYNPIVN 236 Query: 268 PKRAHARQQYILLRMRQLGYITDQQYTQAKDEELKVKTDSTAFGVHAEYVSEMARQLVYA 327 PKRAH RQQYIL RM +G+IT QY +A EELKV+ + + +HAEYV+EM RQ+VYA Sbjct: 237 PKRAHIRQQYILQRMLDVGFITKAQYEKAAKEELKVRAPGSQYNIHAEYVNEMVRQMVYA 296 Query: 328 QFKDDTYTRGLNVFTTITKADQDAAYLALRRGIIDYERRHGYRGPEGYMEIPTASKEEAD 387 Q+K+DTYT+G NV+TT+ ADQ AAYLA+R+G++DY+RRHGYRGPE +E+P A+ E+ Sbjct: 297 QYKEDTYTQGFNVYTTVNAADQQAAYLAVRKGVMDYDRRHGYRGPEETIELP-ANAEDRA 355 Query: 388 DAIESELADHPDSDDLIAAVVLEATTKEITAVLATGDEIKISGSGLGFAGNLLSEKAPPQ 447 AI+ + DHPD+DD++AAVV+ A+ K+I AVL +GD+I I G L F + LSEKA Sbjct: 356 AAIDDVMDDHPDNDDILAAVVVAASPKQIDAVLRSGDKISIKGEALRFVASALSEKAKKD 415 Query: 448 KKIKRGAIIRVFQDGK-NWIVTQMPEVESAFISLDPNDGSIRSLVGGFDFNRNKFNHVIQ 506 +I GA+IRV QD K NW V Q+P+VE+A +S+ P DG+IR+LVGGFDF +N FNHV Q Sbjct: 416 IRIGPGALIRVMQDAKQNWQVAQLPQVEAALVSVVPQDGAIRALVGGFDFTQNNFNHVTQ 475 Query: 507 AWRQPGSSFKPFIYSSSLEKGLSPATIINDAPLTFTQTGGQV-WQPKNYGGKFEGPISMR 565 AWRQPGS+FKPFIYS++LEKG +PA+I+NDAP+++ GQ W+P++ GPI++R Sbjct: 476 AWRQPGSTFKPFIYSAALEKGFAPASIVNDAPVSYPAGPGQPNWEPRD-DETPAGPITVR 534 Query: 566 RALQKSINLVSIRILERVGVKYAQEYIT-RFGFDADKNPPYLTLALGAGAVTPLQMVGAY 624 ALQKS+NLV+IR L+ +GV YA+++IT +GFDADK PPYL +ALGAG PLQ+ AY Sbjct: 535 TALQKSVNLVAIRTLDYIGVPYAKDFITGTYGFDADKVPPYLPMALGAGQTNPLQLSAAY 594 Query: 625 SVFANGGYKINPYLISKITDNNGNTLSEAQPARSGDEANRVIDARNAFVMDSMLRDVVRH 684 SVFANGGYK+ PYLIS+ITD G LS+A+P G+ A RV+ RN+++M SML+ V + Sbjct: 595 SVFANGGYKVQPYLISQITDARGQALSKAEPLIVGNGAPRVVSERNSYMMTSMLKSVAQG 654 Query: 685 GTAVKALSLKRTDLAGKTGTTNDSMDAWFAGYQPNLVGIAWMGYDQPKSLGDRETGGGLA 744 GT ++ S+ R+DLAGKTGTTND++D WFAGYQ L + WMGYDQPKSLG +E G LA Sbjct: 655 GTGARSNSIGRSDLAGKTGTTNDAVDGWFAGYQRTLATVVWMGYDQPKSLGSKEFGAQLA 714 Query: 745 LPIWISYMQKALKDVPMVDRTVPSGLIESGGEYYYAEYPPS 785 LPIW+ YM KALK VP D VP G+ GE YY + P+ Sbjct: 715 LPIWVDYMAKALKGVPQFDLPVPEGIRFIDGEPYYDNFTPN 755 >gi|300312242|ref|YP_003776334.1| penicillin-binding 2 protein [Herbaspirillum seropedicae SmR1] Length = 753 Score = 97.4 bits (241), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 85/287 (29%), Positives = 140/287 (48%), Gaps = 23/287 (8%) Query: 474 ESAFISLDPNDGSIRSLVGGFDFNRNKFN-HVIQAWRQPGSSFKPFIYSSSLEKG-LSPA 531 + A I +D G + + VG N + A RQ GS+ KPF+Y ++EK L+ A Sbjct: 307 DGAIIVIDNATGDVLAWVGSSGPLSAAANVDGVVAQRQAGSTLKPFLYELAIEKKWLTAA 366 Query: 532 TIINDAPLTFTQTGGQVWQPKNYGGKFEGPISMRRALQKSINLVSIRILERVGVKYAQEY 591 ++++D+P+ + G ++ P+NY +++G +S+R +L S+N+ ++R L V + Sbjct: 367 SLLDDSPVNLATSAG-LYIPQNYDKQYKGLVSLRTSLGSSLNIPAVRTLVMVTPERFFRR 425 Query: 592 ITRFGFDADKNPPY--LTLALGAGAVTPLQMVGAYSVFANGGYKINPYLISKITDNNGNT 649 + GF+ ++ Y +LALG+ VT + AY V AN G + P L +++ + Sbjct: 426 LQALGFNLRESGDYYGYSLALGSADVTLANLANAYRVLANQG-RYTP-LRTRLDE----- 478 Query: 650 LSEAQPARSGDEANRVIDARNAFVMDSMLRD---VVRHGTAVKALSLKRTDLAGKTGTTN 706 AR+ A +V+DA A+++ +L D R ALS R A KTGT+ Sbjct: 479 ------ARTPARATQVMDADAAYIVGDILSDRSARARTFGLENALS-TRIWTAVKTGTSK 531 Query: 707 DSMDAWFAGYQPNLVGIAWMGYDQPKSLGDRETGGGLALPIWISYMQ 753 D D W GY W+G + D +G A P+W MQ Sbjct: 532 DMRDNWCVGYSTRYTVGVWVGNASGAPMWD-VSGVTGAAPVWQEVMQ 577 Score = 87.0 bits (214), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 55/173 (31%), Positives = 89/173 (51%), Gaps = 15/173 (8%) Query: 112 EERRNLVHIKDIPDIMKKAVLAIEDDRFYEHGGVDYQGIVRASFANL-SGGGGGASTITQ 170 E + V ++D+ ++ A++A ED RFY+H G+D+ + +++ NL + GASTIT Sbjct: 61 ERKLAWVRLEDVSPALRHALIASEDRRFYQHSGIDWSAVAASAWGNLWNSKTRGASTITM 120 Query: 171 QVA----RNFFLTSNEPTFLQKASRKFLYEIPLAWKIERNLTKDQILEVYMNQIYLGQRA 226 Q+A + S + QK S+ ++ + +ERN KDQILE Y+N + Sbjct: 121 QLAGLIYDDLRRKSGARSLTQKVSQAWIAQ-----SLERNWRKDQILEAYLNLVAFRGEL 175 Query: 227 YGFSSAAQIYFGKQLKELTIAESAMLAGLPKAPSAYNPVVNPKRAHARQQYIL 279 G + ++I FGK L E+A+ L +AP+A P+R R IL Sbjct: 176 VGVHALSRIMFGKHPSGLDQGEAAIAVALIRAPNA-----TPRRVADRACAIL 223 >gi|300309743|ref|YP_003773835.1| monofunctional biosynthetic peptidoglycan transglycosylase [Herbaspirillum seropedicae SmR1] Length = 235 Score = 77.8 bits (190), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 55/162 (33%), Positives = 83/162 (51%), Gaps = 8/162 (4%) Query: 123 IPDIMKKAVLAIEDDRFYEHGGVDYQGIVRASFANLSGGG--GGASTITQQVARNFFLTS 180 I + +K+A++A ED F H GVD++ + +A N G G STITQQ+A+N FL S Sbjct: 70 ISNNLKRAIIASEDSNFSSHDGVDWEALQKAYEKNEKKGKVVAGGSTITQQLAKNLFL-S 128 Query: 181 NEPTFLQKASRKFLYEIPLAWKIERNLTKDQILEVYMNQIYLGQRAYGFSSAAQIYFGKQ 240 E ++L+K E + + +E L K +I E+Y+N + G +G +AAQ Y+ Sbjct: 129 GERSYLRKGQ-----EFIITYMLEFLLDKQRIFEIYLNVVEWGNGVFGAEAAAQHYYRIP 183 Query: 241 LKELTIAESAMLAGLPKAPSAYNPVVNPKRAHARQQYILLRM 282 L ++A LA + P Y+ AR IL RM Sbjct: 184 AASLGAEQAARLAVMLPRPRYYDRNRGSGYLAARTSLILRRM 225 >gi|300309672|ref|YP_003773764.1| cell division FtsI protein/penicillin-binding protein 2 [Herbaspirillum seropedicae SmR1] Length = 592 Score = 57.8 bits (138), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 85/341 (24%), Positives = 139/341 (40%), Gaps = 69/341 (20%) Query: 449 KIKRGAIIRV-FQDGKNWIVTQMPEVESAFISLDPNDGSIRSLVGGFDFNRNKFNHVIQA 507 K K G II V + G+ + +P + +PND RS++ G N V+ Sbjct: 265 KAKAGGIIVVDAKTGEVLALANLP-------TYNPND---RSVLTGAQLR----NRVVTD 310 Query: 508 WRQPGSSFKPFIYSSSLEKG-LSPATIINDAPLTFTQTGGQVWQPKNYGGKFEGPISMRR 566 +PGS+ KPF + +L+ ++P+T+ AP T + +G P+++ + Sbjct: 311 TFEPGSTMKPFTVALALDTHRVTPSTVFQTAPGKMTIGTATIGDSHAHG-----PLTVAQ 365 Query: 567 ALQKSINLVSIRILERVGVKYAQEYITRFGFD-----------ADKNPPY--------LT 607 ++KS N+ + +I + + E T GF A + PY T Sbjct: 366 IIEKSSNIGTAKIALGMPPEEMWEMFTTVGFGQQPKFGFPGAVAGRVRPYKSWRPIEQAT 425 Query: 608 LALGAG-AVTPLQMVGAYSVFANGGYKINPYLISKITDNNGNTLSEAQPARSGDEANRVI 666 ++ G G +V+ +Q+ AY +FA G I P +K+TD+ RVI Sbjct: 426 MSYGHGISVSLIQLAHAYLIFARNG-DIIPLSFTKVTDS--------------PIGQRVI 470 Query: 667 DARNAFVMDSMLRDVVRHGTAVKALSLKRTDLAGKTGTT-NDSMDAWFAGYQPNLVGIAW 725 A A M ML VV G + + GKTGT + Y + GIA Sbjct: 471 SADTALQMRRMLETVVAPGGTAPQAQVPGYRVGGKTGTAYKIEGGKYVRKYVASFSGIAP 530 Query: 726 MG---------YDQPKSLGDRETGGGLALPIWISYMQKALK 757 M D+P+ + GG +A P++ + AL+ Sbjct: 531 MSDPRLIISVMIDEPQG---QHYGGPVAGPVFANVAANALR 568 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.134 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,513,722 Number of extensions: 110054 Number of successful extensions: 316 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 303 Number of HSP's successfully gapped: 6 Length of query: 816 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 723 Effective length of database: 1,179,515 Effective search space: 852789345 Effective search space used: 852789345 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 135 (56.6 bits)