BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0169.0044.p (407 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312271|ref|YP_003776363.1| 2-oxoglutarate dehydrogenase E3... 728 0.0 gi|300310787|ref|YP_003774879.1| 2-oxoglutarate dehydrogenase E3... 261 6e-71 gi|300312608|ref|YP_003776700.1| glutathione reductase [Herbaspi... 138 9e-34 >gi|300312271|ref|YP_003776363.1| 2-oxoglutarate dehydrogenase E3 component lipoamide dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 728 bits (1879), Expect = 0.0, Method: Compositional matrix adjust. Identities = 368/402 (91%), Positives = 380/402 (94%) Query: 1 MSKNFDVVVIGGGPGGYIAAIRAAQLGFNTACIDEWKNEKGGPAPGGTCTNVGCIPSKAL 60 MSKNFDVVVIGGGPGGYIAAIRAAQLGFNTACIDEWKNEKGGPAPGGTCTNVGCIPSKAL Sbjct: 1 MSKNFDVVVIGGGPGGYIAAIRAAQLGFNTACIDEWKNEKGGPAPGGTCTNVGCIPSKAL 60 Query: 61 LQSSEHYEHAGHAFKDHGIDVKGLSLNLPQMLGRKNTVVKQNNDGILYLFKKNKVTFFHG 120 LQSSEHYEHA H F +HGI+VKGL LNL +MLGRKNTVVKQNNDGILYLFKKNKV+FFHG Sbjct: 61 LQSSEHYEHASHGFAEHGIEVKGLGLNLEKMLGRKNTVVKQNNDGILYLFKKNKVSFFHG 120 Query: 121 RGSFVKGDANGYEIKVAGAAEESITAKQVIVATGSNARALPGAPFDEELILSNTGALAIT 180 RGSFVKGDANGYEIKVAGAAEE+ITAK VIVATGSNARALPGA FDE+LILSNTGAL+IT Sbjct: 121 RGSFVKGDANGYEIKVAGAAEETITAKHVIVATGSNARALPGAEFDEKLILSNTGALSIT 180 Query: 181 EVPKKLGVIGAGVIGLEMGSVWRRLGAEVTVLEALPTFLGAVDEQIAKEAQKLLAKQGLT 240 EVPKKLGVIGAGVIGLEMGSVWRRLGAEVTVLEALPTFLGAVDEQIAKEAQKLL KQGL Sbjct: 181 EVPKKLGVIGAGVIGLEMGSVWRRLGAEVTVLEALPTFLGAVDEQIAKEAQKLLTKQGLA 240 Query: 241 VNLGVKIGDIKAGKKDVSVDYTDDKGNAQKAVFDKLIVSIGRTPNTTGLNAEAVGLKLDE 300 VNLGVKIG IKAGK VSV+YTD KG+A FDKLIVSIGRTPNT GLNAE VGLKLDE Sbjct: 241 VNLGVKIGAIKAGKNSVSVEYTDAKGDAHTGEFDKLIVSIGRTPNTIGLNAEGVGLKLDE 300 Query: 301 RGFIAVDGDCKTNLPNVWAVGDVVRGPMLAHKAEEEGVAVAERIAGQHGHVNFNTIPWVI 360 RGFIAVDGDCKTNLPNVWAVGDVVRGPMLAHKAEEEGVAVAERIAGQHGHVNFNTIPWVI Sbjct: 301 RGFIAVDGDCKTNLPNVWAVGDVVRGPMLAHKAEEEGVAVAERIAGQHGHVNFNTIPWVI 360 Query: 361 YTSPEIAWVGKTEQQLKADGIAYKAGTFPFLANGRARAPGXS 402 YTSPEIAWVGKTEQQLKA+G+ YKAGTFPFLANGRARA G + Sbjct: 361 YTSPEIAWVGKTEQQLKAEGVQYKAGTFPFLANGRARALGDT 402 >gi|300310787|ref|YP_003774879.1| 2-oxoglutarate dehydrogenase E3 component lipoamide dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 596 Score = 261 bits (668), Expect = 6e-71, Method: Compositional matrix adjust. Identities = 154/400 (38%), Positives = 228/400 (57%), Gaps = 13/400 (3%) Query: 6 DVVVIGGGPGGYIAAIRAAQLGFNTACIDEWKNEKGGPAPGGTCTNVGCIPSKALLQSSE 65 DV+V+GGGPGGY AA RAA LG NT ++ GG C NVGCIPSKALL + Sbjct: 128 DVMVLGGGPGGYSAAFRAADLGLNTVIVEREATL------GGVCLNVGCIPSKALLHVAA 181 Query: 66 HYEHAGHAFKDHGIDVKGLSLNLPQMLGRKNTVVKQNNDGILYLFKKNKVTFFHGRGSFV 125 + A HG+ +++ ++ K+ V+ G+ + K KV +G G F Sbjct: 182 VIDETA-AMASHGVTFGKPQIDIDKLRAYKDKVIGTMTGGLAGMAKARKVQVVNGDGQF- 239 Query: 126 KGDANGYEIKVAGAAEESITAKQVIVATGSNARALPGAPFDEELILSNTGALAITEVPKK 185 N E+ A +++ + K I+A GS+ LP P D I+ +TGAL + +VPK+ Sbjct: 240 -AGPNHIEVTAADGSKKVVQFKHAIIAAGSSVVNLPFVPQDPR-IVDSTGALELRQVPKR 297 Query: 186 LGVIGAGVIGLEMGSVWRRLGAEVTVLEALPTFLGAVDEQIAKEAQKLLAKQGLTVNLGV 245 + VIG G+IGLEM +V+ LGA + V+E + + D + K QK K+ V + Sbjct: 298 MLVIGGGIIGLEMATVYSTLGARIDVVEMMDGLMQGADRDMVKVWQKFNEKRFDNVMVKT 357 Query: 246 KIGDIKAGKKDVSVDYTDDKGNA---QKAVFDKLIVSIGRTPNTTGLNAEAVGLKLDERG 302 K ++A + + V + + A + ++D ++V++GR+PN L+A+ G+ + +RG Sbjct: 358 KTVAVEALPEGIKVTFEAAEAGATAPEPQLYDLVLVAVGRSPNGKKLSADKAGVIVSDRG 417 Query: 303 FIAVDGDCKTNLPNVWAVGDVVRGPMLAHKAEEEGVAVAERIAGQHGHVNFNTIPWVIYT 362 FIAVD +TN+P+++A+GD+V PMLAHKA EG AE IAG+ + + IP V YT Sbjct: 418 FIAVDKQMRTNVPHIFAIGDLVGQPMLAHKAVHEGHVAAEAIAGEKSFFDASVIPSVAYT 477 Query: 363 SPEIAWVGKTEQQLKADGIAYKAGTFPFLANGRARAPGXS 402 PE+AWVG TE + KA GI + G FP+ A+GRA A G S Sbjct: 478 DPEVAWVGVTEDEAKAKGIKIEKGHFPWAASGRAVANGRS 517 >gi|300312608|ref|YP_003776700.1| glutathione reductase [Herbaspirillum seropedicae SmR1] Length = 458 Score = 138 bits (347), Expect = 9e-34, Method: Compositional matrix adjust. Identities = 114/385 (29%), Positives = 189/385 (49%), Gaps = 27/385 (7%) Query: 1 MSK-NFDVVVIGGGPGGYIAAIRAAQLGFNTACIDEWKNEKGGPAPGGTCTNVGCIPSKA 59 MS+ ++D+ IGGG GG AA A+Q G EKG GGTC N+GCIP K Sbjct: 1 MSQYDYDLFTIGGGSGGVRAARFASQAGARVGLA-----EKGDL--GGTCVNLGCIPKK- 52 Query: 60 LLQSSEHYEHAGHAFKDHGIDVKGL-SLNLPQMLGRKNTVVKQNNDGILYLFKKNKVTFF 118 L+ S HY +G + G S + ++ K+ + ND L L KV+ Sbjct: 53 LMSYSAHYHEEFADAAGYGWTLNGQPSFDWSALMANKDREIAHLNDIYLRLLDNAKVSLH 112 Query: 119 HGRGSFVKGDANGYEIKVAGAAEESITAKQVIVATGS--NARALPGAPFDEELILSNTGA 176 G F K + + + + V G + TA+ ++VATG + A+PGA EL +++ Sbjct: 113 RG---FAKVE-DAHTVNVDG---QRFTARHILVATGGRPDKPAIPGA----ELGITSDDF 161 Query: 177 LAITEVPKKLGVIGAGVIGLEMGSVWRRLGAEVTVLEALPTFLGAVDEQIAKEAQKLLAK 236 + +P++ V+G G I +E+ S+ LG EVT++ L +D + +AK Sbjct: 162 FHLKALPQRAVVLGGGYIAVELASILNGLGCEVTLVYRRERLLRNMDADLGIHLADEMAK 221 Query: 237 QGLTVNLGVKIGDI---KAGKKDVSVDYTDDKGNAQKAVFDKLIVSIGRTPNTTGLNAEA 293 +G+ + I I +AG+ + T N + ++ + GRT NT GL + Sbjct: 222 KGIRIVFNASIEAIEAEQAGEASEASVKTVRLTNGEALSAQCVLFATGRTANTAGLGLQE 281 Query: 294 VGLKLDERGFIAVDGDCKTNLPNVWAVGDVVRGPMLAHKAEEEGVAVAERIAGQHGH-VN 352 G+KL G I V+ D ++++P++ AVGDV+ L A E +AV R+ G+ ++ Sbjct: 282 AGVKLKANGAIEVNQDFESSVPSILAVGDVIDRVALTPVALAEAMAVVSRLFGKGERGMS 341 Query: 353 FNTIPWVIYTSPEIAWVGKTEQQLK 377 + IP +++ P + VG +E++ + Sbjct: 342 YANIPTAVFSHPNVGTVGLSEEEAR 366 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.136 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,249,286 Number of extensions: 54556 Number of successful extensions: 225 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 223 Number of HSP's successfully gapped: 4 Length of query: 407 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 320 Effective length of database: 1,207,925 Effective search space: 386536000 Effective search space used: 386536000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)