BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0169.0031.p (802 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312283|ref|YP_003776375.1| aconitate hydratase [Herbaspiri... 1491 0.0 gi|300311598|ref|YP_003775690.1| aconitate hydratase [Herbaspiri... 614 e-176 gi|300312596|ref|YP_003776688.1| 3-isopropylmalate dehydratase l... 93 1e-19 >gi|300312283|ref|YP_003776375.1| aconitate hydratase [Herbaspirillum seropedicae SmR1] Length = 903 Score = 1491 bits (3860), Expect = 0.0, Method: Compositional matrix adjust. Identities = 734/799 (91%), Positives = 766/799 (95%), Gaps = 6/799 (0%) Query: 1 MSRNTLNTLKEFSISGSKKGKFYSLPALEKKLGINISRLPVSIRIVLESVLRNCDGQKVT 60 MSRNTLNTLKEF ISGSKKGKFYSLPAL+K LG+NISRLPVSIRIVLESVLRNCDGQKVT Sbjct: 1 MSRNTLNTLKEFKISGSKKGKFYSLPALQKSLGVNISRLPVSIRIVLESVLRNCDGQKVT 60 Query: 61 EEHVKQLANWGAKAPRVDEIPFVVARVVLQDFTGVPLLADLAAMRNVAKKLGKNPKNIEP 120 EEHVKQLANWGAKA RVDEIPFVVARVVLQDFTGVPLLADLAAMRNVA KLGKNPKNIEP Sbjct: 61 EEHVKQLANWGAKAARVDEIPFVVARVVLQDFTGVPLLADLAAMRNVASKLGKNPKNIEP 120 Query: 121 LVPVDLVVDHSVQIDHFREKKALDLNMKLEFQRNNERYQFMKWGMQAFDTFGVVPPGFGI 180 LVPVDLVVDHSVQIDHFREKKALDLNMKLEF RNNERYQFMKWGMQAFDTFGVVPPGFGI Sbjct: 121 LVPVDLVVDHSVQIDHFREKKALDLNMKLEFSRNNERYQFMKWGMQAFDTFGVVPPGFGI 180 Query: 181 VHQVNLEYLARGVHNKTGIYYPDTLVGTDSHTTMINGIGVVGWGVGGIEAEAGMLGQPVY 240 VHQVNLEYLARGVHNKTG+YYPDTLVGTDSHTTMINGIGVVGWGVGGIEAEAGMLGQPVY Sbjct: 181 VHQVNLEYLARGVHNKTGVYYPDTLVGTDSHTTMINGIGVVGWGVGGIEAEAGMLGQPVY 240 Query: 241 FLTPDVVGVNLTGQLREGVTATDLVLTITELLRKTKVVGKFVEFFGEGTKSLSLTDRATI 300 FLTPDVVGVNLTG LREGVTATDLVLTITELLR+TKVVGKFVEFFGEGT+SLSLTDRATI Sbjct: 241 FLTPDVVGVNLTGALREGVTATDLVLTITELLRQTKVVGKFVEFFGEGTESLSLTDRATI 300 Query: 301 ANMAPEYGATMGFFPVDEATIDYFKGTGRTKAEIDAFESYFKAQNLFGIPKAGDIDFTNE 360 ANMAPEYGATMGFFPVD+ATI+YFKGTGRTKAEIDAFE+YFKAQ L+G+PKAG ID+T E Sbjct: 301 ANMAPEYGATMGFFPVDDATIEYFKGTGRTKAEIDAFEAYFKAQELYGVPKAGQIDYTQE 360 Query: 361 VSLDLGTVAPSLAGPKRPQDRIEIGNVKSNFADLFVKPVAENGFNKKIEDLNKSYTNADG 420 VSLDLGTVAPSLAGPKRPQDRIEIGNVKS F+DLF KP AENGFNKK EDL SY NADG Sbjct: 361 VSLDLGTVAPSLAGPKRPQDRIEIGNVKSTFSDLFTKPTAENGFNKKAEDLGASYKNADG 420 Query: 421 IDVKNGDVLIAAITSCTNTSNPSVLLAAGLLAKKAVEAGLKVAPHIKTSLAPGSRVVTKY 480 +D+ NGDVLIAAITSCTNTSNPSVLLAAGLLAKKAVEAGLKVAPHIKTSLAPGSRVVTKY Sbjct: 421 VDLHNGDVLIAAITSCTNTSNPSVLLAAGLLAKKAVEAGLKVAPHIKTSLAPGSRVVTKY 480 Query: 481 LEAAGLLGYLEKLGFGVTAYGCTTCIGNAGDLTPAMNEAIVKNDIVASAVLSGNRNFEAR 540 LEAAGLL YLEKLGFGVTAYGCTTCIGNAGDLTPAMNEAIVKND+VA+AVLSGNRNFEAR Sbjct: 481 LEAAGLLPYLEKLGFGVTAYGCTTCIGNAGDLTPAMNEAIVKNDVVAAAVLSGNRNFEAR 540 Query: 541 IHPNIRSNFLASPPLVVAYAIAGNVNVDLMTQPVGKGKGGKEIFLGDIWPTSQEIEKLLK 600 IHPNIR+NFLASPPLVVAYAIAGNV DLMT+PVGKGKGGK+I+LGDIWPTSQEIEKLLK Sbjct: 541 IHPNIRANFLASPPLVVAYAIAGNVTRDLMTEPVGKGKGGKDIYLGDIWPTSQEIEKLLK 600 Query: 601 FAMNAKVFKENYADVKGAPGKLWEAIKGVAAGQVYNWPTSTYIAEPPFFESFTEVPKAAA 660 FAMNAK FKENYADVKGAPGKLWEAIKGVA G+VYNWP+STYIAEPPFFE+F+E PKAAA Sbjct: 601 FAMNAKTFKENYADVKGAPGKLWEAIKGVAKGEVYNWPSSTYIAEPPFFENFSEEPKAAA 660 Query: 661 TGISGARALGVFGDSITTDHISPAGSIKDSSPAGKWLQANGVLKADFNSYGSRRGNHEIM 720 GI+GARALGVFGDSITTDHISPAGSIK+SSPAGKWLQANGVLKADFNSYGSRRGNHEIM Sbjct: 661 AGITGARALGVFGDSITTDHISPAGSIKESSPAGKWLQANGVLKADFNSYGSRRGNHEIM 720 Query: 721 MRGTFANVRIKNLMVPG------VEGGVTVHQPSGEQMAIYDAAMKYVNDGVPTMVFGGE 774 MRGTFANVRIKNLM+P VEGG+T+HQPSGE+M+IYDAAMKYV +G PTMVFGGE Sbjct: 721 MRGTFANVRIKNLMIPAKEDGSRVEGGITIHQPSGEEMSIYDAAMKYVAEGTPTMVFGGE 780 Query: 775 EYGTGSSRDWAAKGTQLLG 793 EYGTGSSRDWAAKGTQLLG Sbjct: 781 EYGTGSSRDWAAKGTQLLG 799 >gi|300311598|ref|YP_003775690.1| aconitate hydratase [Herbaspirillum seropedicae SmR1] Length = 873 Score = 614 bits (1583), Expect = e-176, Method: Compositional matrix adjust. Identities = 334/795 (42%), Positives = 476/795 (59%), Gaps = 42/795 (5%) Query: 6 LNTLKEFSISGSKKGKFYSLPALEKKLGINISRLPVSIRIVLESVLRNCDGQKVTEEHVK 65 +NT + G++ F + A+E LP + R++ E+++R CD +T+ ++ Sbjct: 1 MNTQYRKQLPGTRLDYFDARAAVEAIRPGAWDTLPYTSRVLAENLVRRCDPATLTDS-LR 59 Query: 66 QLANWGAKAPRVDEIPFVVARVVLQDFTGVPLLADLAAMRNVAKKLGKNPKNIEPLVPVD 125 QL + R + P+ ARVV D G L DLA +R+ +G +P + P+VPV Sbjct: 60 QLI----ERKRELDFPWFPARVVCHDILGQTALVDLAGLRDAIADMGGDPAKVNPVVPVQ 115 Query: 126 LVVDHSVQIDHF-REKKALDLNMKLEFQRNNERYQFMKWGMQAFDTFGVVPPGFGIVHQV 184 L+VDHS+ ++ + +A N +E +RN +R+ F+ W AF+ V+PPG GI+HQ+ Sbjct: 116 LIVDHSLAVECGGDDPQAFVKNRAIEDRRNEDRFHFIDWTKLAFENVDVIPPGNGIMHQI 175 Query: 185 NLEYLARGVHNKTGIYYPDTLVGTDSHTTMINGIGVVGWGVGGIEAEAGMLGQPVYFLTP 244 NLE ++ +H K G+ +PDTLVGTDSHT ++ +GV+ GVGG+EAE MLG+ + P Sbjct: 176 NLEKMSPVIHAKDGVAFPDTLVGTDSHTPHVDALGVIAIGVGGLEAENVMLGRASWMRLP 235 Query: 245 DVVGVNLTGQLREGVTATDLVLTITELLRKTKVVGKFVEFFGEGTKSLSLTDRATIANMA 304 D++GV LTG+ + G+TATD+VL++TE LRK KVVG ++EF+G+G SL+L DRATI+NMA Sbjct: 236 DIIGVELTGRRQPGITATDIVLSLTEFLRKQKVVGAYLEFYGDGAASLTLGDRATISNMA 295 Query: 305 PEYGATMGFFPVDEATIDYFKGTGRTKAEIDAFESYFKAQNLFGIPKAGDIDFTNEVSLD 364 PEYGAT F +D+ TIDY K TGR ++ E+Y K L+ A +++ + D Sbjct: 296 PEYGATAAMFSIDQQTIDYLKLTGREDEQVKLVETYAKEAGLWSDTLA-KVEYERVLRFD 354 Query: 365 LGTVAPSLAGPKRPQDRIEIGNVKSNFADLFVKPVAENGFNKKIEDLNKSYTNADGIDVK 424 L TV +LAGP P R L V +A G K+E N G + Sbjct: 355 LSTVVRTLAGPSNPHKR------------LPVSELAAQGIAGKVE-------NEPG-KMP 394 Query: 425 NGDVLIAAITSCTNTSNPSVLLAAGLLAKKAVEAGLKVAPHIKTSLAPGSRVVTKYLEAA 484 +G V+IAAITSCTNTSNP ++AAGLLA+ A GL P +K+SLAPGS+ V YLE A Sbjct: 395 DGAVIIAAITSCTNTSNPRNVIAAGLLARNANRLGLARKPWVKSSLAPGSKAVELYLEEA 454 Query: 485 GLLGYLEKLGFGVTAYGCTTCIGNAGDLTPAMNEAIVKNDIVASAVLSGNRNFEARIHPN 544 GL LEKLGFG+ A+ CTTC G +G L P + + I+ D+ ++AVLSGNRNF+ RIHP Sbjct: 455 GLTADLEKLGFGIVAFACTTCNGMSGALDPKIQQEIIDRDLYSTAVLSGNRNFDGRIHPY 514 Query: 545 IRSNFLASPPLVVAYAIAGNVNVDLMTQPVGKGKGGKEIFLGDIWPTSQEIEKLLKFAMN 604 + FLASPPLVVAYAIAG + D+ +G G+EI L DIWP+ +EI+ ++ A+ Sbjct: 515 AKQAFLASPPLVVAYAIAGTIRFDIEQDVLGVTAEGREIRLKDIWPSDEEIDAVVTAAVK 574 Query: 605 AKVFKENYADVKGAPGKLWEAIKGVAAGQVYNW-PTSTYIAEPPFFESFTEVPKAAATGI 663 + F+ Y + A + + +Y+W P STYI PP++E A + Sbjct: 575 PQQFRNVYTPMFAA-----RVDRSQSVSPLYDWRPQSTYIRRPPYWEGAL----AGERTL 625 Query: 664 SGARALGVFGDSITTDHISPAGSIKDSSPAGKWLQANGVLKADFNSYGSRRGNHEIMMRG 723 SG R L V GD+ITTDH+SP+ +I S AG++L G+ + DFNSY + RG+H R Sbjct: 626 SGMRPLAVLGDNITTDHLSPSNAILLDSAAGEYLAKMGLPEEDFNSYATHRGDHLTAQRA 685 Query: 724 TFANVRIKNLMVPGVEGGV-----TVHQPSGEQMAIYDAAMKYVNDGVPTMVFGGEEYGT 778 TFAN ++ N MV +G V +P G+ M +++ Y+ P ++ G +YG Sbjct: 686 TFANPKLFNEMVKKADGSVQQGSLARLEPEGKVMRMWETIETYMERKQPLIIIAGADYGQ 745 Query: 779 GSSRDWAAKGTQLLG 793 GSSRDWAAKG +L G Sbjct: 746 GSSRDWAAKGVRLAG 760 >gi|300312596|ref|YP_003776688.1| 3-isopropylmalate dehydratase large subunit [Herbaspirillum seropedicae SmR1] Length = 466 Score = 92.8 bits (229), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 105/387 (27%), Positives = 160/387 (41%), Gaps = 40/387 (10%) Query: 171 FGVVPPGFGIVHQVNLEYLARGVHNKTGIYYPD-TLVGTDSHTTMINGIGVVGWGVGGIE 229 FG+ GIVH + E G P T+V DSHT+ + G+G E Sbjct: 98 FGMNDKRQGIVHVIGPE---------QGATLPGMTVVCGDSHTSTHGAFAALAHGIGTSE 148 Query: 230 AEAGMLGQPVYFLTPDVVGVNLTGQLREGVTATDLVLTITELLRKTKVVGKFVEFFGEGT 289 E + Q + + V + G L GVTA D+VL + + G +EF G Sbjct: 149 VEHVLATQTLLARKSKAMLVQVDGALPAGVTAKDIVLAVIGKIGTAGGTGYAIEFAGSTI 208 Query: 290 KSLSLTDRATIANMAPEYGATMGFFPVDEATIDYFKGTGRTKA--EIDAFESYFKAQNLF 347 +SLS+ R T+ NMA E GA G D+ T++Y KG + + + SY+++ L Sbjct: 209 RSLSMEGRMTVCNMAIEAGARAGMVAFDDTTLEYLKGRPFSPSGPHWERAVSYWRS--LH 266 Query: 348 GIPKAGDIDFTNEVSLDLGTVAPSLAGPKRPQDRIEIGNVKSNFADLFVKPVAENGFNKK 407 P A F V+L+ + P + P+ + + + + D P +G K Sbjct: 267 SDPGA---KFDMVVTLNAAEIKPQVTWGTSPEMVVAV-DARVPDPDKEKDPTKRDGMEKA 322 Query: 408 IEDLNKSYTNADGIDVKNGDVLIAAITSCTNTSNPSVLLAAGLLAKKAVEAGLKVAPHIK 467 + + N D++ V I SCTN+ + AA ++ K + +K+A Sbjct: 323 LAYMALK-PNTPIEDIRIDKVFIG---SCTNSRIEDLREAAKVVRGKFRASNVKLA---- 374 Query: 468 TSLAPGSRVVTKYLEAAGLLGYLEKLGFGVTAYGCTTCIG-NAGDLTPAMNEAIVKNDIV 526 + PGS +V + E GL GF GC+ C+ NA L P A Sbjct: 375 -MVVPGSGLVKEQAEREGLDKIFRDAGFEWREPGCSMCLAMNADRLEPGERCAS------ 427 Query: 527 ASAVLSGNRNFEARIHPNIRSNFLASP 553 + NRNFE R R++ L SP Sbjct: 428 -----TSNRNFEGRQGAGGRTH-LVSP 448 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.136 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,434,894 Number of extensions: 103253 Number of successful extensions: 279 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 272 Number of HSP's successfully gapped: 3 Length of query: 802 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 709 Effective length of database: 1,179,515 Effective search space: 836276135 Effective search space used: 836276135 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)