BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0169.0026.p (347 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312286|ref|YP_003776378.1| signal-transducing histidine ki... 471 e-134 gi|300309957|ref|YP_003774049.1| chemotaxis histidine kinase [He... 215 3e-57 gi|300311033|ref|YP_003775125.1| chemotaxis histidine kinase [He... 189 4e-49 gi|300312396|ref|YP_003776488.1| chemotaxis CheA protein [Herbas... 161 6e-41 gi|300313133|ref|YP_003777225.1| CheA-like histidine kinase [Her... 131 8e-32 >gi|300312286|ref|YP_003776378.1| signal-transducing histidine kinase CheA protein [Herbaspirillum seropedicae SmR1] Length = 697 Score = 471 bits (1212), Expect = e-134, Method: Compositional matrix adjust. Identities = 247/335 (73%), Positives = 272/335 (81%), Gaps = 10/335 (2%) Query: 1 VNTDDLKISQAV-AGRSKEEEQGYGFFAPFVPQDATDEERGYGFFAPFVPMNATDKERGY 59 VN DLKISQAV + S ++E+GYGFF FV Q+ + +GYGFF + ++E+GY Sbjct: 189 VNVADLKISQAVESAPSADQERGYGFFDNFVTQEERERTQGYGFFDNHTAIEDKEREQGY 248 Query: 60 GFFEPFVPQGETEQAKQAAEAAA--HTDDDPPALAVTEGGEKKAKGEKPATV----HESQ 113 GFF PFVP + K AE + H DD P LAVTE + K K A HES Sbjct: 249 GFFAPFVPL-DISAKKPDAETLSQPHPDDAP--LAVTEHAAAEKKAAKAAAAAAAGHESS 305 Query: 114 SIRVGIEKVDQLINLVGELVITQAMIEQRTGSLDPMLHEHLLNSVSQLTRNTRDLQESVM 173 SIRV +EKVDQLINLVGELVITQAMIEQR G LDPM HEHLLNSVSQL+RNTRDLQESVM Sbjct: 306 SIRVSVEKVDQLINLVGELVITQAMIEQRVGKLDPMTHEHLLNSVSQLSRNTRDLQESVM 365 Query: 174 SIRMMPMDYVFSRFPRMVRDLAAKLGKRVEFVTHGAATELDKGLIERIVDPLTHLVRNSI 233 SIRMMPMDYVFSRFPRMVRDLA KLGK+VEFVTHGAATELDKGLIERIVDPLTHLVRNSI Sbjct: 366 SIRMMPMDYVFSRFPRMVRDLAHKLGKQVEFVTHGAATELDKGLIERIVDPLTHLVRNSI 425 Query: 234 DHGIEIPETRLAAGKTDYGTLSLSAEHQGGNIIIEVTDDGGGLNRDKILAKAKQQGMAVS 293 DHGIE+P+ R A+GK++ GTLSLSAEHQGGNIIIEVTDDGGGLNR+KILAKAKQQGM V+ Sbjct: 426 DHGIELPDARRASGKSEAGTLSLSAEHQGGNIIIEVTDDGGGLNREKILAKAKQQGMPVT 485 Query: 294 DNLSDGDVWQLIFEPGFSTAEQVTDVSGRGVGMDV 328 DNLSD +VWQLIFEPGFSTAEQVTDVSGRGVGMDV Sbjct: 486 DNLSDAEVWQLIFEPGFSTAEQVTDVSGRGVGMDV 520 >gi|300309957|ref|YP_003774049.1| chemotaxis histidine kinase [Herbaspirillum seropedicae SmR1] Length = 725 Score = 215 bits (548), Expect = 3e-57, Method: Compositional matrix adjust. Identities = 120/261 (45%), Positives = 169/261 (64%), Gaps = 14/261 (5%) Query: 76 QAAEAAAHTDDDPPALAV----TEGGEKKAKGEKPATVHESQSIRVGIEKVDQLINLVGE 131 Q+ EAA+ T D A AV EG ++ + E + I++ + K+DQLI+LVGE Sbjct: 291 QSLEAASATPSDALAAAVEPMAAEGVLQRPRALDERRAQEQKFIKIEVSKLDQLIDLVGE 350 Query: 132 LVITQA---MIEQRTGSLDPMLHEHLLNSVSQLTRNTRDLQESVMSIRMMPMDYVFSRFP 188 LVI A +I +R E ++ L RD + +++RM+ ++ VF RFP Sbjct: 351 LVIAGASARLIARRRKDSQ---FEEATQAIDTLMEQIRD---AALTLRMVQINEVFQRFP 404 Query: 189 RMVRDLAAKLGKRVEFVTHGAATELDKGLIERIVDPLTHLVRNSIDHGIEIPETRLAAGK 248 R+VRD+A LGK ++ V G+ TELDK ++ER+ DPL H+VRN+IDHGIE RLAAGK Sbjct: 405 RIVRDIARDLGKDIDLVMTGSDTELDKSMVERLADPLMHIVRNAIDHGIEPAAERLAAGK 464 Query: 249 TDYGTLSLSAEHQGGNIIIEVTDDGGGLNRDKILAKAKQQGMAVSD-NLSDGDVWQLIFE 307 +L L+A H+ G+++IEV DDG G++R+ ILAKA QG+A D LSD ++++ +FE Sbjct: 465 PAKASLQLNAIHESGSVVIEVMDDGRGMDREAILAKAVAQGLASPDAELSDAEIYRFVFE 524 Query: 308 PGFSTAEQVTDVSGRGVGMDV 328 PGFSTA QVT++SGRGVGMDV Sbjct: 525 PGFSTARQVTELSGRGVGMDV 545 >gi|300311033|ref|YP_003775125.1| chemotaxis histidine kinase [Herbaspirillum seropedicae SmR1] Length = 837 Score = 189 bits (479), Expect = 4e-49, Method: Compositional matrix adjust. Identities = 98/227 (43%), Positives = 150/227 (66%), Gaps = 12/227 (5%) Query: 112 SQSIRVGIEKVDQLINLVGELVITQAMI-------EQRTGSLDPMLHEHLLNSVSQLTRN 164 ++S++V K+D+L+NL+GE+V+++ + E + G + L + + + R Sbjct: 448 NKSVKVEQVKIDRLMNLIGEMVVSKNALPYLAQRAEDQFGVRE--LAREIKAQYAVINRI 505 Query: 165 TRDLQESVMSIRMMPMDYVFSRFPRMVRDLAAKLGKRVEFVTHGAATELDKGLIERIVDP 224 ++Q+++M +RMMP+ VF RFPR+VRD++ KLGK V+ V G TE DK +IE + DP Sbjct: 506 AEEMQDAIMQVRMMPVSVVFQRFPRLVRDISRKLGKEVQLVLVGEETEADKNIIEALADP 565 Query: 225 LTHLVRNSIDHGIEIPETRLAAGKTDYGTLSLSAEHQGGNIIIEVTDDGGGLNRDKILAK 284 L H+VRNS+DHG+E PE R A+GK G+L++SA H+G ++I + DDG G++ I K Sbjct: 566 LIHIVRNSLDHGLEGPEQRRASGKPALGSLTISAAHEGDQVVIVIEDDGKGIDPALIKRK 625 Query: 285 AKQQGM---AVSDNLSDGDVWQLIFEPGFSTAEQVTDVSGRGVGMDV 328 A ++G+ A + +SD D L+F GFSTAE V+D+SGRGVGMDV Sbjct: 626 AYEKGLIDEAQLERISDQDAVNLVFSSGFSTAEVVSDLSGRGVGMDV 672 >gi|300312396|ref|YP_003776488.1| chemotaxis CheA protein [Herbaspirillum seropedicae SmR1] Length = 638 Score = 161 bits (408), Expect = 6e-41, Method: Compositional matrix adjust. Identities = 89/225 (39%), Positives = 139/225 (61%), Gaps = 11/225 (4%) Query: 113 QSIRVGIEKVDQLINLVGE--------LVITQAMIEQRTGSLDPMLHEHLLNSVSQLTRN 164 +SIR+ K+D L+ + GE V+ + + + + L L ++ + +R Sbjct: 249 ESIRIDAGKLDALLEVAGESVQAANQAAVLLEKLQQFKFEGTAAALMSTLAETLGRASRY 308 Query: 165 TRDLQESVMSIRMMPMDYVFSRFPRMVRDLAAKLGKRVEFVTHGAATELDKGLIERIVDP 224 + +LQ + +S RM P+ +F +FPR+VR+LA LGK V+ V GA TE+D+ +++ + DP Sbjct: 309 SSELQRATLSTRMQPVGRLFQKFPRLVRELAKDLGKDVDLVITGAETEVDRVVVDSLYDP 368 Query: 225 LTHLVRNSIDHGIEIPETRLAAGKTDYGTLSLSAEHQGGNIIIEVTDDGGGLNRDKILAK 284 L H++RNS+DHGIE+ R+AAGK T+SL+A G +++IEV DDG G++ D + +K Sbjct: 369 LVHMLRNSLDHGIEV--DRVAAGKPTKATISLNAWQAGSSVMIEVFDDGKGMDPDMLRSK 426 Query: 285 AKQQGMAVSDN-LSDGDVWQLIFEPGFSTAEQVTDVSGRGVGMDV 328 A +G+ L+ + QL+F PGFST E + VSGRGVGMDV Sbjct: 427 ALAKGLINEHQALTADEALQLVFLPGFSTKEVASSVSGRGVGMDV 471 >gi|300313133|ref|YP_003777225.1| CheA-like histidine kinase [Herbaspirillum seropedicae SmR1] Length = 744 Score = 131 bits (329), Expect = 8e-32, Method: Compositional matrix adjust. Identities = 68/171 (39%), Positives = 104/171 (60%) Query: 158 VSQLTRNTRDLQESVMSIRMMPMDYVFSRFPRMVRDLAAKLGKRVEFVTHGAATELDKGL 217 + Q +TR+LQ+ +M RM+ + + R +VR +AA+ GK + ++D+ + Sbjct: 394 LRQQAAHTRELQQELMYARMVKFNSMEVRMQHLVRQVAAETGKPLALDLSNCRLQIDRAI 453 Query: 218 IERIVDPLTHLVRNSIDHGIEIPETRLAAGKTDYGTLSLSAEHQGGNIIIEVTDDGGGLN 277 +ER++ PL HL+RN+ HGIE E RLA GK G L++ A H+G +I V+DDG GL+ Sbjct: 454 LERLMGPLEHLLRNAAVHGIESAEQRLAEGKPAVGRLTVQATHEGNEAVIRVSDDGRGLD 513 Query: 278 RDKILAKAKQQGMAVSDNLSDGDVWQLIFEPGFSTAEQVTDVSGRGVGMDV 328 + I KA + G A S + LIF+PG ST+ QV+ ++GRG+GMDV Sbjct: 514 LEAIRQKALELGAAGLAEASQARLADLIFQPGLSTSPQVSALAGRGIGMDV 564 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.132 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 960,443 Number of extensions: 37936 Number of successful extensions: 133 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 118 Number of HSP's successfully gapped: 7 Length of query: 347 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 261 Effective length of database: 1,212,660 Effective search space: 316504260 Effective search space used: 316504260 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 131 (55.1 bits)