BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0169.0020.p (241 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312291|ref|YP_003776383.1| hypothetical protein Hsero_2987... 243 8e-66 >gi|300312291|ref|YP_003776383.1| hypothetical protein Hsero_2987 [Herbaspirillum seropedicae SmR1] Length = 159 Score = 243 bits (621), Expect = 8e-66, Method: Compositional matrix adjust. Identities = 120/159 (75%), Positives = 136/159 (85%) Query: 83 LLLLFWSTFRSQVPFYPSGQATWDAVAGLLPADQPLRVIDIGSGLGGAVLYLSRLRPDSR 142 +LLLFWSTFRSQVPFYPSGQA WDAVAGLLPAD+ +R +DIGSGLGGAVL L+R RP+S Sbjct: 1 MLLLFWSTFRSQVPFYPSGQAAWDAVAGLLPADRAIRFLDIGSGLGGAVLDLARRRPESE 60 Query: 143 FVGIELAPLPWLVSQVRALLGRSRGAFVRGDYTRLDFSDYDVIFAYLSPAAMTALWQKAR 202 F+GIE+APLPWLVS++RA LGR R FVRGDY LDF+DYDVIFAYLSPAAM ALW+KAR Sbjct: 61 FIGIEIAPLPWLVSRLRAALGRHRCRFVRGDYLLLDFADYDVIFAYLSPAAMVALWRKAR 120 Query: 203 VEMRPGTLLLSYEFIIEDMQPDVVVHPKENGPALYGWRM 241 EMRPG+LLLSYEF I PDVV+ P+ GP L+GWRM Sbjct: 121 AEMRPGSLLLSYEFHIPGATPDVVIQPEGGGPVLHGWRM 159 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.330 0.144 0.474 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 680,929 Number of extensions: 26756 Number of successful extensions: 131 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 131 Number of HSP's successfully gapped: 1 Length of query: 241 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 158 Effective length of database: 1,226,865 Effective search space: 193844670 Effective search space used: 193844670 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 129 (54.3 bits)