BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0169.0016.p
         (263 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300312294|ref|YP_003776386.1| cystathionine beta-lyase [Herba...   435   e-123
gi|300312963|ref|YP_003777055.1| o-acetylhomoserine sulfhydrylas...    93   3e-20
gi|300312582|ref|YP_003776674.1| O-acetylhomoserine sulfhydrylas...    85   7e-18

>gi|300312294|ref|YP_003776386.1| cystathionine beta-lyase
           [Herbaspirillum seropedicae SmR1]
          Length = 392

 Score =  435 bits (1119), Expect = e-123,   Method: Compositional matrix adjust.
 Identities = 203/257 (78%), Positives = 230/257 (89%)

Query: 6   TPQTSLIHSEYVAPDNFGALPVAIHHASTVLFPNVAAMRARNWKEKSGYTYGLHGTPTTF 65
           TPQTSL+HS Y APD F A PV IHHASTVLFP+VAAMR+R+W+EK GYTYGLHGTPT+F
Sbjct: 10  TPQTSLVHSSYRAPDGFEAFPVGIHHASTVLFPDVAAMRSRDWREKLGYTYGLHGTPTSF 69

Query: 66  TLEARLAEIEGGRHCRIVPSGLAAIALVNLALLKTGDDVLLPDNVYGPNRDQAVWLERDF 125
            LEARLAEIEGG++CR+ PSGLAAIA+VN A LK+GDDVLLPDNVYGP++D   WLERDF
Sbjct: 70  VLEARLAEIEGGKYCRVTPSGLAAIAMVNFAFLKSGDDVLLPDNVYGPSKDLGQWLERDF 129

Query: 126 GITARFYDPLIGAGIADLIQDNTRLIWTEAPGSVSMEVSDVPAICSAARARGVPVAMDNT 185
            ITARFYDP+IG G+A+LI+  TRL+WTEAPGSVSMEV D+PA+C AA A+GV VA+DNT
Sbjct: 130 QITARFYDPMIGEGLAELIRPTTRLVWTEAPGSVSMEVPDIPALCRAAHAKGVLVAIDNT 189

Query: 186 WSAGLAFRAFDHGVDIVMQALTKYQSGGSDVLMGAVITRDDELNHKMELAHMRLGFGVGM 245
           WSAG+AF+AF+HGVDIVMQA+TKYQSGGSDVLMGAVI RD  LN K+ELAHMRLGFGVGM
Sbjct: 190 WSAGIAFKAFEHGVDIVMQAVTKYQSGGSDVLMGAVIVRDQALNEKLELAHMRLGFGVGM 249

Query: 246 DDVYMVLRSLPSMRLRF 262
           DDVYMVLRSL SMRLRF
Sbjct: 250 DDVYMVLRSLSSMRLRF 266


>gi|300312963|ref|YP_003777055.1| o-acetylhomoserine sulfhydrylase
           [Herbaspirillum seropedicae SmR1]
          Length = 429

 Score = 92.8 bits (229), Expect = 3e-20,   Method: Compositional matrix adjust.
 Identities = 53/158 (33%), Positives = 79/158 (50%)

Query: 52  SGYTYGLHGTPTTFTLEARLAEIEGGRHCRIVPSGLAAIALVNLALLKTGDDVLLPDNVY 111
            G  Y     PT   LE RLA +EGG     + SG AA+      + + GD+++    +Y
Sbjct: 54  QGNIYSRIMNPTQDVLEKRLAALEGGIGALALASGQAAVTYAIQTIAEAGDNIVSASTLY 113

Query: 112 GPNRDQAVWLERDFGITARFYDPLIGAGIADLIQDNTRLIWTEAPGSVSMEVSDVPAICS 171
           G   +        +G+  RF DP   A    LI D T+ I+ E+ G+    ++D+ A+  
Sbjct: 114 GGTYNLFAHTLPQYGVQTRFADPRQPASFEPLIDDRTKAIYIESIGNPLGNITDIAAVAD 173

Query: 172 AARARGVPVAMDNTWSAGLAFRAFDHGVDIVMQALTKY 209
            A   GVP+ +DNT +     R F+HG DIV+ +LTKY
Sbjct: 174 IAHRHGVPLIVDNTVATPYLLRPFEHGADIVVHSLTKY 211


>gi|300312582|ref|YP_003776674.1| O-acetylhomoserine sulfhydrylase
           [Herbaspirillum seropedicae SmR1]
          Length = 413

 Score = 84.7 bits (208), Expect = 7e-18,   Method: Compositional matrix adjust.
 Identities = 59/220 (26%), Positives = 102/220 (46%), Gaps = 3/220 (1%)

Query: 3   EKYTPQTSLIHSEYVAPDNFGALPVAIHHASTVLFPNVAAMRARNWKEKSGYTYGLHGTP 62
           + Y   T+++HS+       G+L   IH + T  + +   +      ++ GY YG  G P
Sbjct: 5   KTYGFTTTILHSDRQKGIEHGSLHKPIHTSVTFGYEDARQLAEVFQGKQPGYRYGRQGNP 64

Query: 63  TTFTLEARLAEIEGGRHCRIVPSGLAAIALVNLALLKTGDDVLLPDNVYGPNRDQAVWLE 122
           T   LE ++ ++E G+      +G+AAI  +   LL+ GD V+    ++G     ++W+ 
Sbjct: 65  TVAALEDKITKMEDGKSTICFATGMAAIGAIVQGLLREGDHVVSSAFLFG--NTNSLWMT 122

Query: 123 RDF-GITARFYDPLIGAGIADLIQDNTRLIWTEAPGSVSMEVSDVPAICSAARARGVPVA 181
               G      D      +   I  NTRL++ E   +   +V+D+  I    R RG+   
Sbjct: 123 VGAQGAKVSMVDATDVKNVEAAITANTRLVFVETIANPRTQVADLKRIGELCRERGILYV 182

Query: 182 MDNTWSAGLAFRAFDHGVDIVMQALTKYQSGGSDVLMGAV 221
           +DNT ++   FR    G  +V+ +LTK   G  + L GA+
Sbjct: 183 VDNTMTSPYLFRPKTVGAGLVVNSLTKSIGGHGNALGGAL 222


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.322    0.137    0.414 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 896,553
Number of extensions: 37690
Number of successful extensions: 98
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 97
Number of HSP's successfully gapped: 3
Length of query: 263
Length of database: 1,619,870
Length adjustment: 83
Effective length of query: 180
Effective length of database: 1,226,865
Effective search space: 220835700
Effective search space used: 220835700
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 129 (54.3 bits)