BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0169.0007.p (245 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312304|ref|YP_003776396.1| permease [Herbaspirillum serope... 240 9e-65 >gi|300312304|ref|YP_003776396.1| permease [Herbaspirillum seropedicae SmR1] Length = 260 Score = 240 bits (612), Expect = 9e-65, Method: Compositional matrix adjust. Identities = 140/217 (64%), Positives = 164/217 (75%) Query: 1 LAGFVKGVVGLGLPTVAVGLLGLFMPPLQAAALLIVPSLVTNVWQLLAGGRFLWLLRRLW 60 LAGFVKG +GLGLPTVAVGLL L MPP+QAA LLIVPS+VTN WQLL GG L+RRLW Sbjct: 24 LAGFVKGSIGLGLPTVAVGLLSLMMPPVQAAPLLIVPSMVTNGWQLLTGGALRALVRRLW 83 Query: 61 LMLLGIVAGTLLGANFMAPDASGRATVFLGVALVAYAVLGLAAIRFSVSAGREKTWSPLI 120 +L GIV GTL G +FM D RA + LG+ALV YA+ GLA+ SVS +E+ WSP + Sbjct: 84 TLLAGIVLGTLAGGSFMNADGGRRAIIVLGLALVLYALAGLASWTPSVSPAQEQWWSPAV 143 Query: 121 GLITGLVTAATAVFVIPAVPYLQALNLEKDELIQALGLSFTVSTIAMAIGLAQGGALQME 180 GL TGLVTAAT VFVIPAVPYLQAL L +++LIQALGLSFTVST+A+A+ L QGGA Sbjct: 144 GLATGLVTAATGVFVIPAVPYLQALGLPREQLIQALGLSFTVSTLALAVNLGQGGAFSGG 203 Query: 181 GAAISVMALLPALGGMFIGQWMRGKISATLFRRCFFI 217 S++ LLPAL GM IG +RG++ A FRRCFFI Sbjct: 204 VGWASLVLLLPALLGMSIGARVRGRVQAVTFRRCFFI 240 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.143 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 593,932 Number of extensions: 19953 Number of successful extensions: 143 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 143 Number of HSP's successfully gapped: 1 Length of query: 245 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 162 Effective length of database: 1,226,865 Effective search space: 198752130 Effective search space used: 198752130 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 129 (54.3 bits)