BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0168.0054.p (151 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310189|ref|YP_003774281.1| transmembrane protein [Herbaspi... 187 4e-49 gi|300314042|ref|YP_003778134.1| signal transduction protein con... 108 1e-25 gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protei... 103 7e-24 gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [... 100 6e-23 gi|300312026|ref|YP_003776118.1| signal transduction protein con... 98 3e-22 >gi|300310189|ref|YP_003774281.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 706 Score = 187 bits (474), Expect = 4e-49, Method: Compositional matrix adjust. Identities = 86/108 (79%), Positives = 94/108 (87%) Query: 13 ATLRVTSSIGIAVYPNSGDTVDALMKNADAAMYEAKQNGRNTYRFFEPAMHASAMRHLTV 72 ATLRVTSSIGIAVYPNSGD+VDALMKNADAAMYEAKQ+GRNTYRFFEPAMHASAMRHL V Sbjct: 378 ATLRVTSSIGIAVYPNSGDSVDALMKNADAAMYEAKQSGRNTYRFFEPAMHASAMRHLQV 437 Query: 73 RHALQLAVEHNQLSLHFQPKFEGRQHTLTGVEALLRWDHPELGSMSPT 120 R ALQ A++ Q LHFQPK+ G LTG+EALLRW HP+LG M+PT Sbjct: 438 RQALQQAIDEQQFRLHFQPKYRGASKELTGLEALLRWTHPQLGEMAPT 485 >gi|300314042|ref|YP_003778134.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 948 Score = 108 bits (271), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 48/106 (45%), Positives = 70/106 (66%) Query: 14 TLRVTSSIGIAVYPNSGDTVDALMKNADAAMYEAKQNGRNTYRFFEPAMHASAMRHLTVR 73 TL +T S+G+A YPN G + L+K+AD AMY KQ GRN ++F+ PAM+ A+ L + Sbjct: 636 TLFMTCSVGVACYPNDGTDGETLIKHADIAMYRGKQTGRNNFQFYTPAMNEQALERLRIE 695 Query: 74 HALQLAVEHNQLSLHFQPKFEGRQHTLTGVEALLRWDHPELGSMSP 119 L+ A++H + LH+QPK + + G EAL+RW HP+LG +SP Sbjct: 696 GDLRNALDHGEFLLHYQPKVDLASGRVIGSEALIRWQHPQLGMVSP 741 >gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 695 Score = 103 bits (257), Expect = 7e-24, Method: Compositional matrix adjust. Identities = 55/115 (47%), Positives = 74/115 (64%), Gaps = 4/115 (3%) Query: 15 LRVTSSIGIAVYPNSGDTVDALMKNADAAMYEAKQNGRNTYRFFEPAMHASAMRHLTVRH 74 LRV++S+GIA+YP G T L+ NADAAMY K++GRN Y FFEP+M+A+A + L Sbjct: 383 LRVSASVGIAIYPEDGATRHDLVINADAAMYHTKRSGRNGYHFFEPSMNANAQQQLQWLQ 442 Query: 75 ALQLAVEHNQLSLHFQPKFEGRQHTLTGVEALLRWDHPELGSMSPTXNCDQVQGF 129 L++A+E Q L +QPKF + G EALLRW+HP G ++P DQ G Sbjct: 443 DLRVALERGQFRLVYQPKFSLPAGPVLGAEALLRWEHPVHGLVAP----DQFIGL 493 >gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1036 Score = 100 bits (248), Expect = 6e-23, Method: Compositional matrix adjust. Identities = 49/105 (46%), Positives = 68/105 (64%), Gaps = 3/105 (2%) Query: 16 RVTSSIGIAVYPNSGDTVDALMKNADAAMYEAKQNGRNTYRFFEPAMHASAMRHLTVRHA 75 R T+SIG V+ +VD LMK AD AMY+AK+ GRN RFF+PAM + + T+ Sbjct: 723 RTTASIGATVFLGRAASVDELMKQADLAMYKAKETGRNALRFFDPAMQTAVLERATLEAG 782 Query: 76 LQLAVEHNQLSLHFQPK-FEGRQHTLTGVEALLRWDHPELGSMSP 119 L+ A++ NQL LH+QP+ EG + +TG E L+RW HP+ G + P Sbjct: 783 LRKAIDGNQLLLHYQPQVVEGGR--VTGAEVLVRWQHPQRGMVPP 825 >gi|300312026|ref|YP_003776118.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1106 Score = 98.2 bits (243), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 50/133 (37%), Positives = 73/133 (54%), Gaps = 11/133 (8%) Query: 17 VTSSIGIAVYPNSGDTVDALMKNADAAMYEAKQNGRNTYRFFEPAMHASAMRHLTVRHAL 76 ++ SIGIA YP GD L+KNAD AMY AK +G++ YRFF ++ + + + + L Sbjct: 790 LSVSIGIATYPGDGDNAQTLIKNADIAMYRAKHHGKDDYRFFSDEINTNTVERIALEAEL 849 Query: 77 QLAVEHNQLSLHFQPKFEGRQHTLTGVEALLRWDHPELGSMSP---------TXNCDQVQ 127 + A+E + +H+QPK E + + G EAL+RW HP G + P T DQ+ Sbjct: 850 RRAIERAEFVVHYQPKIELAEGRIVGAEALVRWQHPLRGLLPPGEFITLAEETRLIDQIG 909 Query: 128 GFLLARPAAARDV 140 LL A RD+ Sbjct: 910 --LLVLDVACRDI 920 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.132 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 466,878 Number of extensions: 17249 Number of successful extensions: 110 Number of sequences better than 1.0e-08: 13 Number of HSP's gapped: 106 Number of HSP's successfully gapped: 13 Length of query: 151 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 74 Effective length of database: 1,255,275 Effective search space: 92890350 Effective search space used: 92890350 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 126 (53.1 bits)