BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0168.0034.p (494 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310177|ref|YP_003774269.1| C4-dicarboxylate transport sens... 470 e-133 gi|300313806|ref|YP_003777898.1| PAS/PAC HisKA ATPase_c domains ... 113 3e-26 gi|300310677|ref|YP_003774769.1| two component transmembrane sen... 82 7e-17 gi|300312655|ref|YP_003776747.1| C4-dicarboxylate transport sens... 76 5e-15 gi|300313027|ref|YP_003777119.1| two component transmembrane sen... 75 1e-14 >gi|300310177|ref|YP_003774269.1| C4-dicarboxylate transport sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 674 Score = 470 bits (1210), Expect = e-133, Method: Compositional matrix adjust. Identities = 231/290 (79%), Positives = 257/290 (88%) Query: 10 RLIAREIVNEHLKKDQFRSRLNALLRNNREFYRVTWFGVDGKPIASTDDFPVPRHELSMP 69 R+I REI N L ++ FRSRLNALLRNNREFYRVTWF +G+P+ASTDDFP+ R +LS Sbjct: 86 RIIGREITNGRLHQEHFRSRLNALLRNNREFYRVTWFNAEGRPVASTDDFPITRADLSKS 145 Query: 70 SQQAEEHARTIRRPIYSAPAVPSGIAEPVVMDYHIPLFRESQYLGSVVASYSVSRVLNEM 129 S E AR +RRP+YS PAVP GIAEP++MDYH+PLF ++ Y+GS+VASY+V+R+LNEM Sbjct: 146 SLMTGERARQMRRPLYSPPAVPPGIAEPMLMDYHVPLFHDNHYVGSLVASYAVARILNEM 205 Query: 130 VPWWFAQDNEILLTDTDDKVVARRTAGGPGLNVYTHRRELELPGLTLRLHTNSTKSAPKL 189 VPWWFAQDNEI LTDTDD VVARRT+GGPGLNVYTHRRELELPGLTL LHTNSTKSAPKL Sbjct: 206 VPWWFAQDNEISLTDTDDNVVARRTSGGPGLNVYTHRRELELPGLTLVLHTNSTKSAPKL 265 Query: 190 LPNLLVGSVIVLSLGLLWSLWALWRDINRRMTAEEALVHEVSFRSAMENSLVTGMRARDL 249 LPNLLVGSVIVLSLGLLWSLWALWRDINRR AE+AL++E FR+AMENSLVTGMRARDL Sbjct: 266 LPNLLVGSVIVLSLGLLWSLWALWRDINRRTKAEKALLNEAVFRAAMENSLVTGMRARDL 325 Query: 250 EGRITYVNPAFCTMVGIPADELVGKIPPMPYWAPEAMDEYQERFSKVLAG 299 EGRITYVNPAFC MVG A+ELVGKIPPMPYWAPEAMDEYQERFS VLAG Sbjct: 326 EGRITYVNPAFCKMVGFAAEELVGKIPPMPYWAPEAMDEYQERFSNVLAG 375 Score = 273 bits (697), Expect = 3e-74, Method: Compositional matrix adjust. Identities = 134/192 (69%), Positives = 154/192 (80%), Gaps = 4/192 (2%) Query: 295 KVLAGXKKKINTQAQRAGNVIRSVHQFVKKREPTRQPVSIKNIVDNIAPLVELQAQQFLV 354 K L +KI+ QAQRAG+VIRSVHQFVKKREP RQ V ++ ++DN+APL+ELQAQQF V Sbjct: 482 KALQPALEKIHAQAQRAGHVIRSVHQFVKKREPARQRVQMRQLIDNVAPLIELQAQQFFV 541 Query: 355 VVQTAIPADLPDVLGDAVLLEQVLLNLTRNAIQAMAASAPERRILRIAATLDRQTSSQQS 414 V+Q +P LP VLGD +LLEQV+LNLTRN IQAMAA RRILR+AA + R + Sbjct: 542 VLQLQVPRSLPPVLGDQILLEQVILNLTRNGIQAMAAVPSARRILRVAAEVTR----NED 597 Query: 415 TQQSVVVSVIDQGHGIPPEVAAALFSPFFSTKAEGMGMGLNICRTTIEFHGGTLSYADNP 474 +SV VSV DQGHGIP +VAA LFSPFFSTKAEGMGMGLNICRTTIEFHGGTL+Y DNP Sbjct: 598 GAESVTVSVTDQGHGIPDDVAARLFSPFFSTKAEGMGMGLNICRTTIEFHGGTLTYTDNP 657 Query: 475 TGGTIFRFSLPS 486 GGTIFRFSLP+ Sbjct: 658 AGGTIFRFSLPA 669 >gi|300313806|ref|YP_003777898.1| PAS/PAC HisKA ATPase_c domains containing sensor signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 524 Score = 113 bits (283), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 69/189 (36%), Positives = 102/189 (53%), Gaps = 12/189 (6%) Query: 302 KKINTQAQRAGNVIRSVHQFVKKREPTRQPVSIKNIVDNIAPLV--ELQAQQFLVVVQTA 359 + I + QRAG +I + + P + V++ ++ +I L EL+ ++ +++ + Sbjct: 346 RDIIAEGQRAGGIISGLRDLTRNAAPVCERVNLHAVIRHILTLSRSELERREVSLLL-SL 404 Query: 360 IPADLPDVLGDAVLLEQVLLNLTRNAIQAMAASAPERRILRIAATLDRQTSSQQSTQQSV 419 PAD VLGD V L+QV+LNL NAI+AM++ R+L I A+ +Q + Sbjct: 405 TPAD-AFVLGDQVQLQQVMLNLVVNAIEAMSSMPEHARVLSITAS--------SCDEQRI 455 Query: 420 VVSVIDQGHGIPPEVAAALFSPFFSTKAEGMGMGLNICRTTIEFHGGTLSYADNPTGGTI 479 V V D G GI PE +F F++TK GMGMGL ICR+ IE H G L A G + Sbjct: 456 EVKVQDSGEGIAPEAMRRVFDAFYTTKDSGMGMGLAICRSIIENHRGRLRVAAAQPHGAL 515 Query: 480 FRFSLPSLP 488 F F LP +P Sbjct: 516 FSFDLPRVP 524 >gi|300310677|ref|YP_003774769.1| two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 508 Score = 82.4 bits (202), Expect = 7e-17, Method: Compositional matrix adjust. Identities = 53/179 (29%), Positives = 89/179 (49%), Gaps = 9/179 (5%) Query: 307 QAQRAGNVIRSVHQFVKKREPTRQPVSIKNIVDNIAPLVELQAQQFLVVVQTAIPADLPD 366 Q A +I+SV ++V K E + + + ++ + +E++A + V ++ + Sbjct: 333 QVDHAAAIIKSVREYVVKLEQSEGLIDLNEVLAEVRWFIEMRAAEGRVQLEVQASEEALV 392 Query: 367 VLGDAVLLEQVLLNLTRNAIQAMAASAPERRILRIAATLDRQTSSQQSTQQSVVVSVIDQ 426 V + VL+ QV+LNL NAI+ M+ +R R+ +L R+ Q +++V D+ Sbjct: 393 VSCEKVLIGQVILNLAFNAIEEMSHFPENQR--RVCISLARKD-------QQALLTVADE 443 Query: 427 GHGIPPEVAAALFSPFFSTKAEGMGMGLNICRTTIEFHGGTLSYADNPTGGTIFRFSLP 485 G GI LF FFS+K G G+GL +C+ I H G + GT F F+LP Sbjct: 444 GRGIDAGHRERLFDGFFSSKVSGNGIGLALCKNIIARHRGDIWAQPAQVRGTRFCFTLP 502 >gi|300312655|ref|YP_003776747.1| C4-dicarboxylate transport sensor kinase transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 600 Score = 76.3 bits (186), Expect = 5e-15, Method: Compositional matrix adjust. Identities = 58/189 (30%), Positives = 90/189 (47%), Gaps = 14/189 (7%) Query: 304 INTQAQRAGNVIRSVHQFVKKREPTRQPVSIKNIVDNIAPLVELQAQQFLVVVQTAIPAD 363 I++ + G +I + F +K V + ++ + L+ + Q+ + I A Sbjct: 423 ISSASATMGKIIAQLKGFARKSGEAVACVDLSQAIEASSLLLASECQKLGATISIDIRAA 482 Query: 364 LPDVLGDAVLLEQVLLNLTRNAIQAMAASAPERRILRIAATLDRQTSSQQSTQQSVVVSV 423 V GD V EQVL+NL RNA+ A+ E RIA L+ +V + Sbjct: 483 -ACVTGDVVRTEQVLVNLLRNALDAV----EEAEHKRIAVVLEVAGGY-------ALVRI 530 Query: 424 IDQGHGIPPEVAAALFSPFFSTK--AEGMGMGLNICRTTIEFHGGTLSYADNPTGGTIFR 481 D G GI EVA LF PFF+TK ++G+G+GL I + ++ G LS ++ GG F Sbjct: 531 RDSGGGIADEVAPHLFEPFFTTKSSSKGLGLGLAISSSIVQAMNGQLSAHNHEEGGAEFV 590 Query: 482 FSLPSLPVQ 490 LP L ++ Sbjct: 591 VRLPLLKME 599 >gi|300313027|ref|YP_003777119.1| two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 454 Score = 75.1 bits (183), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 56/186 (30%), Positives = 85/186 (45%), Gaps = 17/186 (9%) Query: 305 NTQAQRAGNVIRSVHQFVKKREPTRQPVSIKNIVDNIAPLVELQAQQFLVVVQTAIPADL 364 N QR N+++S+ F + E Q + +D+ + + + + +V+ +L Sbjct: 267 NEGLQRVRNIVQSLKDFSRIGESNWQMTDLHRGLDSTLNIANNELKYKVSIVKQY--GEL 324 Query: 365 PDVLGDAVLLEQVLLNLTRNAIQAMAASAPERRILRIAATLDRQTSSQQSTQQSVVVSVI 424 P V A L QV +NL NA QA++ ER + I V VS+ Sbjct: 325 PLVKCLASELNQVFMNLLVNAAQAIS----ERGTVTICTA---------RAGDKVSVSIS 371 Query: 425 DQGHGIPPEVAAALFSPFFSTK--AEGMGMGLNICRTTIEFHGGTLSYADNPTGGTIFRF 482 D GHGI PE +F PFF+TK G G+GL++ ++ HGG + + P GT F Sbjct: 372 DTGHGIAPENLNRIFEPFFTTKPVGSGTGLGLSLSYGIVKKHGGDIRVSSAPGCGTTFTI 431 Query: 483 SLPSLP 488 LP P Sbjct: 432 ELPIDP 437 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,435,410 Number of extensions: 56196 Number of successful extensions: 271 Number of sequences better than 1.0e-08: 15 Number of HSP's gapped: 269 Number of HSP's successfully gapped: 16 Length of query: 494 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 405 Effective length of database: 1,198,455 Effective search space: 485374275 Effective search space used: 485374275 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)