BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0168.0003.p (553 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312928|ref|YP_003777020.1| transmembrane acyl-CoA dehydrog... 837 0.0 gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 107 3e-24 gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbasp... 92 7e-20 gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [H... 87 4e-18 >gi|300312928|ref|YP_003777020.1| transmembrane acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 824 Score = 837 bits (2162), Expect = 0.0, Method: Compositional matrix adjust. Identities = 406/492 (82%), Positives = 441/492 (89%) Query: 1 MILWLVLFVAGALALMMMRAGAWTWLVAELAWIWIGGMTDQANAVACVLLAILLGVPTLL 60 MI+WL+L +A ALALMMMRA AW WL AE +W+G + VLLAIL PTLL Sbjct: 1 MIIWLILLIASALALMMMRASAWAWLAAEAVLLWLGARLAEIEWPVLVLLAILFFTPTLL 60 Query: 61 VAIKSVRETLITRPALDIFRKILPSMSATERDAIEAGTVWWDAELFSGKPDWDKLMRLPP 120 VAI+SVR+ L+TRPALDIFRKI+P+MSATERDAIEAGTVWWDAELFSGKPDW +L LP Sbjct: 61 VAIRSVRQALMTRPALDIFRKIMPAMSATERDAIEAGTVWWDAELFSGKPDWSRLFALPA 120 Query: 121 PALTAEEQSFLDNEVTQLCAMVSDWDTSVVWQDLQPEAWQFVKDKGFLGMIIPKQYGGKQ 180 P LTAEEQ+FLDNEV QLC MVSDWD +V QDL PEAWQF+KDKGFLGMIIP+QYGGKQ Sbjct: 121 PRLTAEEQAFLDNEVEQLCGMVSDWDATVKTQDLPPEAWQFIKDKGFLGMIIPRQYGGKQ 180 Query: 181 FSAYMHSQVIMKLSSRCSVAAISVMVPNSLGPAELLLHYGTEEQKNHYLPRLARGEEIPC 240 FSAYMHSQV+MKL+SR S AAI+VMVPNSLGPAELLLHYGTE QKNHYLPRLA+G EIPC Sbjct: 181 FSAYMHSQVVMKLASRSSAAAIAVMVPNSLGPAELLLHYGTEVQKNHYLPRLAQGLEIPC 240 Query: 241 FALTSPYAGSDAAAIPDIGVVCKGMHEGREVIGFRVTWAKRYITLAPVATVLGLAFRAHD 300 FALTSPYAGSDAAAIPDIG+VCKG+HEGRE +GFRVTW+KRYITLAP+ATVLGLAFRA D Sbjct: 241 FALTSPYAGSDAAAIPDIGIVCKGVHEGRETLGFRVTWSKRYITLAPIATVLGLAFRASD 300 Query: 301 PDHLLSDNAEPGITCALIPTSHPGVVTGRRHWPLNAVFQNGPTSGENVFIPVDWVIGGQA 360 PD LL E GITCALIPT HPGVVTGRRHWPLNAVFQNGPT G++VFIP++WVIGG A Sbjct: 301 PDGLLGGEREAGITCALIPTDHPGVVTGRRHWPLNAVFQNGPTEGKDVFIPMEWVIGGVA 360 Query: 361 QVGKGWRMLMECLAAGRAISLPSSSVGFSKMAVRGTSAYTAMRRQFKIAIGKFEGIQEAL 420 QVG+GWRMLMECLAAGRAISLPSSSVGFSKMAVRGTS Y AMRRQFKI IGKFEG+QEAL Sbjct: 361 QVGRGWRMLMECLAAGRAISLPSSSVGFSKMAVRGTSGYAAMRRQFKIEIGKFEGVQEAL 420 Query: 421 ARMGGNLYMMDATRRLSSLAVDAGEKPSVISAISKYHVTERGRMLVNDGMDVIGGKGICM 480 ARMGG+LYMMDATRRLS+LAVDAGEKPSVISAISKYH+TERGRM+VNDGMDVIGGKGICM Sbjct: 421 ARMGGHLYMMDATRRLSALAVDAGEKPSVISAISKYHITERGRMVVNDGMDVIGGKGICM 480 Query: 481 GPGNFLARVYQQ 492 GPGNFLARVYQQ Sbjct: 481 GPGNFLARVYQQ 492 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 107 bits (266), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 102/376 (27%), Positives = 171/376 (45%), Gaps = 52/376 (13%) Query: 137 QLCAMVSDWDTSVVWQDLQPEAWQFVKDKGFLGMIIPKQYGGKQFSAYMHSQVIMKLSSR 196 +L +DWD + EA + + + G +GM +P+Q+GG + ++++ Sbjct: 21 RLLPFAADWDRN---HTFPAEALKELAELGAMGMCVPEQWGGAGMDYMSLVLALEEIAAG 77 Query: 197 CSVAAISVMVPNSLGPAELLLHYGTEEQKNHYLPRLARGEEIPCFALTSPYAGSDAAAIP 256 + V V NSL + YG+ QK +L LARGE + CF LT P+ GSDAAAI Sbjct: 78 DGATSTIVSVQNSLACG-ITEKYGSPTQKEQWLKPLARGEMLGCFCLTEPHTGSDAAAIR 136 Query: 257 DIGVVCKGMHEGREVIGFRVTWAKRYITL---APVATVLGLAFRAHDPDHLLSDNAEPGI 313 + +G + I + K++IT A VA V + R+ + GI Sbjct: 137 -----TRAERDGDDFI---LNGTKQFITSGKHAGVAIVFAVTDRS---------AGKKGI 179 Query: 314 TCALIPTSHPGVVTGRRHWPLNAVFQNGPTSGE----NVFIPVDWVIGGQAQVGKGWRML 369 +C L+P PG V GR + Q+ + + N +P ++G + G+G+R+ Sbjct: 180 SCFLVPCDTPGFVVGRSEEKMG---QHASDTVQIMLDNCRVPATALLGKE---GEGYRIA 233 Query: 370 MECLAAGRAISLPSSSVGFSKMAVRGTSAYTAMRRQFKIAIGKFEGIQEALARMGGNLYM 429 + L AGR I + + SVG ++ A +Y R F + I + + + LA M + Sbjct: 234 LSNLEAGR-IGIAAQSVGMARAAFEAAVSYARQRESFGVPIIEHQAVNFRLADMN---TL 289 Query: 430 MDATR----RLSSLAVDAGEKPSVISAISKYHVTERGRMLVNDGMDVIGGKGICMGPGNF 485 +DA R R + L D G ++++K +E + +D + + GG G +F Sbjct: 290 LDAARLMVWRAAQLK-DQGRPCLKEASMAKMFASEAAEKIASDAIQIHGGVGY---TSDF 345 Query: 486 -LARVYQQXGGRDARL 500 + R+Y RD R+ Sbjct: 346 PVERIY-----RDVRI 356 >gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 92.4 bits (228), Expect = 7e-20, Method: Compositional matrix adjust. Identities = 92/358 (25%), Positives = 165/358 (46%), Gaps = 32/358 (8%) Query: 125 AEEQSFLDNEVTQLCAMVSDWDTSVVWQ-DLQPEAWQFVKDKGFLGMIIPKQYGGKQFSA 183 A +QS D ++ + WD + D+ +A + GF G+ P+ GG S Sbjct: 11 AFQQSARDFAAGEMAPFAAKWDEEAHFPLDVIAKAGEL----GFCGLYTPEADGGLGLSR 66 Query: 184 YMHSQVIMKLSSRCSVAAISVMVPNSLGPAELLLHYGTEEQKNHYLPRLARGEEIPCFAL 243 + V +L+ C+ A + + N + + ++ +G K + +LA G +I + L Sbjct: 67 LDATVVFEELARACTSTAAYLTIHNMV--SWMIASWGQPSLKAQWCEQLAAGRKIGSYCL 124 Query: 244 TSPYAGSDAAAIPDIGVVCKGMHEGREVIGFRVTWAKRYITLAPVATVLGLAFRAHDPDH 303 T P +GSDAA++ + G + + +K +I+ A VL + R Sbjct: 125 TEPGSGSDAASLKTTARLEDGH--------YVLNGSKAFISGAGSTDVLVVMART----- 171 Query: 304 LLSDNAEPGITCALIPTSHPGVVTGRRHWPLNAVFQNGPT---SGENVFIPVDWVIGGQA 360 G++ ++P + PGV G++ + + + PT S +NV +P D ++G Sbjct: 172 --GGEGARGVSAIVVPGNAPGVSYGKKESKMG--WNSQPTRTISFDNVKVPADHLLG--- 224 Query: 361 QVGKGWRMLMECLAAGRAISLPSSSVGFSKMAVRGTSAYTAMRRQFKIAIGKFEGIQEAL 420 Q G+G+ M+ L GR I++ + SVG ++ A+ AY R+QF I F+ +Q L Sbjct: 225 QEGQGFIFAMKGLDGGR-INIATCSVGAAQAALDAAHAYMKERKQFGRPIADFQALQFKL 283 Query: 421 ARMGGNLYMMDATRRLSSLAVDAGE-KPSVISAISKYHVTERGRMLVNDGMDVIGGKG 477 A M L RL++ +DAG + + A++K T+ G + N+ + + GG G Sbjct: 284 ADMQTELVAARQMVRLAATKLDAGSPEATTYCAMAKRLATDLGFRVCNEALQLHGGYG 341 >gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 394 Score = 87.0 bits (214), Expect = 4e-18, Method: Compositional matrix adjust. Identities = 84/329 (25%), Positives = 145/329 (44%), Gaps = 32/329 (9%) Query: 159 WQFVKDKGFLGMIIPKQYGGKQFSAYMHSQVIMKLSSRCSVAAISVMVPNSLGPAELLLH 218 W+ + D G LG+ + ++YGG H + ++S + +S ++L ++ + Sbjct: 48 WKKLGDLGVLGITVSEEYGGAGLGYLAHIIAMEEISRASASVGLSYGAHSNLCVNQIKRN 107 Query: 219 YGTEEQKNHYLPRLARGEEIPCFALTSPYAGSDAAAIPDIGVVCKGMHEGREVIGFRVTW 278 G EEQK YLPRL G+ I A++ P AGSD VV + ++ + + Sbjct: 108 -GNEEQKRKYLPRLISGDFIGALAMSEPNAGSD--------VVSMKLRADKKGDRYVLNG 158 Query: 279 AKRYITLAPVATVLGLAFRAHDPDHLLSDNAEPGITCALIPTSHPGVVTGRRHWPLNAVF 338 +K +IT P A VL + + D + G+T L+ + G ++ L+ + Sbjct: 159 SKMWITNGPDADVL-VVYAKTDL-----EAGARGMTAFLVEKGYKGFSVAQK---LDKLG 209 Query: 339 QNGPTSGENVF----IPVDWVIGGQAQVGKGWRMLMECLAAGRAISLPSSSVGFSKMAVR 394 G +GE VF +P + V+GG VG+G +LM L R++ L +G + + Sbjct: 210 MRGSHTGELVFQDCEVPEENVLGG---VGRGVNVLMSGLDFERSV-LSGGPLGIMQACMD 265 Query: 395 GTSAYTAMRRQFKIAIGKFEGIQEALARMGGNLYMMDATRRLSSLAVDAGEKPSVISAIS 454 Y R+QF AIG+F+ +Q LA M + A A D + + A+ Sbjct: 266 VVVPYVHDRKQFGQAIGEFQLMQGKLADMYSTMMACKAYVYAVGQACDRADSADKVRALR 325 Query: 455 K------YHVTERGRMLVNDGMDVIGGKG 477 K + E+ + + + +GG G Sbjct: 326 KDAAGAILYSAEKATWMAGEAIQSLGGNG 354 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.137 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,863,330 Number of extensions: 83209 Number of successful extensions: 216 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 215 Number of HSP's successfully gapped: 4 Length of query: 553 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 463 Effective length of database: 1,193,720 Effective search space: 552692360 Effective search space used: 552692360 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 133 (55.8 bits)