BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0167.0001.p (264 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311081|ref|YP_003775173.1| high-affinity gluconate transpo... 477 e-136 gi|300310424|ref|YP_003774516.1| high-affinity gluconate permeas... 258 4e-70 gi|300311416|ref|YP_003775508.1| gluconate transporter family pr... 180 1e-46 >gi|300311081|ref|YP_003775173.1| high-affinity gluconate transporter (GNT-III system) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 450 Score = 477 bits (1227), Expect = e-136, Method: Compositional matrix adjust. Identities = 242/263 (92%), Positives = 251/263 (95%) Query: 1 VGNTLGHIALVVGLGTMLGKMMAESGGAERIATTMIGAFGEKNVHWAMMTVAFIVGLPVF 60 VGN LGHIALVVGLGTMLGKMMAESGGAERIA TMI AFGEKNVHWAMMTVAFIVGLPVF Sbjct: 61 VGNALGHIALVVGLGTMLGKMMAESGGAERIANTMIKAFGEKNVHWAMMTVAFIVGLPVF 120 Query: 61 FEVGFVLLIPIAFNVAKRTGTSMVLVGIPMVAGLSVVHGLIPPHPAALLAVTAYNADIGR 120 FEVGFVLL+PIAFNVAKRTGT+MVLVGIPMVAGLSVVHGLIPPHPAALLAVTAY+ADIGR Sbjct: 121 FEVGFVLLVPIAFNVAKRTGTNMVLVGIPMVAGLSVVHGLIPPHPAALLAVTAYSADIGR 180 Query: 121 TILFALIVGIPTAIIAGPIFGKLMSKVVIPNPDNPLIGQFVDQGGAKRELPGFGITLFTI 180 TIL+ALIVGIPTAIIAGPIFGKL+SKVVIPNPDNPLI QFVD+G RELPGFGITLFTI Sbjct: 181 TILYALIVGIPTAIIAGPIFGKLISKVVIPNPDNPLISQFVDEGKKDRELPGFGITLFTI 240 Query: 181 LLPVALMLIGSWADLFFAPKTFANDFLRLIGNSVIALLIATLVSFYTFGKLRGFNREAIL 240 LLPVALMLIGSWADLFFAPKTFANDFLRLIGNSVIALLIATLVSF+TFG+ RGF + IL Sbjct: 241 LLPVALMLIGSWADLFFAPKTFANDFLRLIGNSVIALLIATLVSFWTFGRARGFGADQIL 300 Query: 241 KFTNECLGPIASITLVVGAGAGF 263 KFTNECL PIASITLVVGAGAGF Sbjct: 301 KFTNECLAPIASITLVVGAGAGF 323 >gi|300310424|ref|YP_003774516.1| high-affinity gluconate permease [Herbaspirillum seropedicae SmR1] Length = 450 Score = 258 bits (659), Expect = 4e-70, Method: Compositional matrix adjust. Identities = 132/255 (51%), Positives = 182/255 (71%), Gaps = 1/255 (0%) Query: 10 LVVGLGTMLGKMMAESGGAERIATTMIGAFGEKNVHWAMMTVAFIVGLPVFFEVGFVLLI 69 +V+GLGTMLGK+MAESGGA++IA T++ AFG + VHWAMM AF+VG+P+FFE+GFVLLI Sbjct: 71 IVLGLGTMLGKLMAESGGADQIAQTLVRAFGRERVHWAMMMGAFLVGIPLFFEIGFVLLI 130 Query: 70 PIAFNVAKRTGTSMVLVGIPMVAGLSVVHGLIPPHPAALLAVTAYNADIGRTILFALIVG 129 P+ F VA+R+G + +G+PM+A LSV+HGL+PPHP LLA+ + ADIG+TI + L+VG Sbjct: 131 PLVFIVARRSGVPLFKIGMPMLASLSVMHGLVPPHPGPLLAIGIFGADIGKTIFYGLLVG 190 Query: 130 IPTAIIAGPIFGKLMSKVVIPNPDNPLIGQFVDQGGAKRELPGFGITLFTILLPVALMLI 189 +PT IIAGP+FG +S+ V P L+ Q V + + LPGF TL T+L PV LML+ Sbjct: 191 LPTVIIAGPMFGAFISRYVDIKPSEELMAQLVREPQTTK-LPGFATTLITVLAPVFLMLL 249 Query: 190 GSWADLFFAPKTFANDFLRLIGNSVIALLIATLVSFYTFGKLRGFNREAILKFTNECLGP 249 + D+ D + IGN ++ALL A L++ +TFG RGF R+ +LKF ++ L P Sbjct: 250 KTLVDVTLPEGHAMRDLMDFIGNPIVALLAALLLAIWTFGIARGFTRQQVLKFVDQSLAP 309 Query: 250 IASITLVVGAGAGFN 264 A+I L++GAG GF Sbjct: 310 TAAIVLIIGAGGGFK 324 >gi|300311416|ref|YP_003775508.1| gluconate transporter family protein [Herbaspirillum seropedicae SmR1] Length = 456 Score = 180 bits (456), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 104/264 (39%), Positives = 155/264 (58%), Gaps = 4/264 (1%) Query: 2 GNTLGHIALVVGLGTMLGKMMAESGGAERIATTMIGAFGEKNVHWAMMTVAFIVGLPVFF 61 G+ LG +++ LG MLG +MAESG A+RI T++ + W M VA +VGLP+FF Sbjct: 69 GSLLGEAGIIIALGAMLGALMAESGAADRIVNTLLRYARGSFIPWMMALVALVVGLPLFF 128 Query: 62 EVGFVLLIPIAFNVAKRTGTSMVLVGIPMVAGLSVVHGLIPPHPAALLAVTAYNADIGRT 121 EVG V++ PI F +A+R+ ++ V IP +AG++ +H L+PPHP L+AV+A +AD+G T Sbjct: 129 EVGLVMMAPIIFVMARRSELPIMRVAIPALAGMTTLHALLPPHPGPLIAVSALHADLGTT 188 Query: 122 ILFALIVGIPTAIIAGPIFGKLMS-KVVIPNPDNPLIGQFVDQGGAKRELPGFGITLFTI 180 +L IV IP IIAGP++G ++ ++ + PD IG+ + + P F + L TI Sbjct: 189 MLLGFIVAIPAVIIAGPLYGNFIAPRLNLAEPDQ--IGKLFSAREGQSQ-PSFLVALVTI 245 Query: 181 LLPVALMLIGSWADLFFAPKTFANDFLRLIGNSVIALLIATLVSFYTFGKLRGFNREAIL 240 LLPV LML + A ++ PKT + L G +IAL + L + G +G +R + Sbjct: 246 LLPVVLMLGRTVARIWVTPKTELFEMLNFFGEPIIALTVTVLFAVVVLGWGQGSSRFEVG 305 Query: 241 KFTNECLGPIASITLVVGAGAGFN 264 L PIA + L +GAG G Sbjct: 306 ATLRRALPPIAGLLLTIGAGGGLK 329 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.145 0.433 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 887,523 Number of extensions: 39824 Number of successful extensions: 190 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 188 Number of HSP's successfully gapped: 3 Length of query: 264 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 181 Effective length of database: 1,226,865 Effective search space: 222062565 Effective search space used: 222062565 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 130 (54.7 bits)