BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0165.0060.p (1882 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310626|ref|YP_003774718.1| filamentous hemagglutinin prote... 138 4e-33 gi|300312548|ref|YP_003776640.1| filamentous hemagglutinin outer... 121 6e-28 >gi|300310626|ref|YP_003774718.1| filamentous hemagglutinin protein [Herbaspirillum seropedicae SmR1] Length = 2658 Score = 138 bits (348), Expect = 4e-33, Method: Compositional matrix adjust. Identities = 193/660 (29%), Positives = 290/660 (43%), Gaps = 141/660 (21%) Query: 63 PAFAAPIADPAAPIRFQPQVTQSTGVGGGVPVVNITAPNAAGISLNQYSQFNVDAIGLIL 122 P + +ADP A +P V + G P+V I P+AAG+S NQYSQFNV G IL Sbjct: 35 PGYPQIVADPNAGAGKRPVVDNTAN---GRPLVQIATPSAAGVSHNQYSQFNVGPNGAIL 91 Query: 123 NNSLISGGSTLGGTVTANGNLRGRTASQIINQVTTQGSASRIAGTIEVFGTQADVIIANP 182 NN+ + GG V AN NL+G A I+N+VT+ S S++ G EV G +A+VIIANP Sbjct: 92 NNARDVVLTQQGGYVGANPNLQGGAARVILNEVTST-SPSQLRGYTEVAGQRAEVIIANP 150 Query: 183 NGINCTACGFINTPRVVPGAGIE--------------------GSAGV-------IDLIG 215 NGI+C CGFINT R V G+ GS G+ +DLI Sbjct: 151 NGISCDGCGFINTSRGVLTTGVPLFGGNGSLEAFRVSRGAIQIGSGGLNGANTDQLDLIA 210 Query: 216 GSIGVNASINAGQQINLISGQQLVS------EAVAGQGKVDSDYSVATNGSSPNFSGPDP 269 S+ VN + G ++NL++G V+ +AG+G P Sbjct: 211 RSVQVNGEL-WGNRLNLVTGANRVNYGDLGLSVIAGEGNA-------------------P 250 Query: 270 TLQIDATAFGAMNAGQIKVVATTAGLGVKA-GVLNANNGNVTIDANGNVNATNVYGSQGA 328 T+ +D G M A +I +V T G+GV++ G L A G+ T+ + G + Sbjct: 251 TIGVDVARMGGMYAQKIMLVGTENGVGVRSLGTLAAQAGSFTLSSQGQI----------- 299 Query: 329 TIRADGSVSAGTVTSYGAASFNAGTDVTVGQITAANNAQITGRNIAIGNAVVGGALTVNG 388 + S S V + N G + A NAQ+ G I NA G L+ G Sbjct: 300 ALSGKTSASDDIVLKSAVGTSNDGV------LYAGRNAQLQGG--VIENA---GTLSAQG 348 Query: 389 SGNVTTSQSGAIVGDAQFTAGGGFTNTGNWTAGGNLNISANTVGNALSSQLSGLGSTTVT 448 + ++T +Q + T G GN GNL ++A+ G+ L++ ++ G+ Sbjct: 349 NLDITAAQL-----RSTGTLAAGVDQAGNVAQAGNLTVTAS--GDVLATGVNRAGADLQL 401 Query: 449 AGSMVNYGTLYGDNLRVTLSGGLDNTFGSMLAKTAMVANVGSLVSNLGGTMYVGDPNAAA 508 G G +D + S A + A ++ LGG A Sbjct: 402 VG------------------GKIDLSHSSSSAGRKLGAQASDALT-LGG---------AT 433 Query: 509 NAAPAGDLALTVSSSGSSFNNLGGLIAASNGLTVNAVNAALDMSGTATGRLNAGGALAVN 568 AGDL L G++ G + +S +NAV A L G G + + GA+ V Sbjct: 434 VETLAGDLEL----GGATITLDGAKLTSSADARINAV-AGLSNRG---GEITSAGAMNVT 485 Query: 569 AGLLVNSGSWLASG----------SSVSLSGVNGIVNAGVVQSAGNLTLSSSAGVITNGG 618 A + NS +++ S+ S+S G + AG + ++ VI NG Sbjct: 486 AASVDNSAGMISAATGGAGVLSLRSAGSISNGQGRIAAGAALNLAAASIQGQGSVIANGD 545 Query: 619 ALVAGQDLTLNGTTNNIAGAILHAERDLTI--NGAGTNAGTMEALRNVSLQGSDFNNSKG 676 A ++ Q L NN AG+++ + RDL I +GA N G ++A+ ++L S +N+ G Sbjct: 546 ATLSLQSL------NNEAGSLIQSNRDLRITTSGATGNQGALKAIGALNLTASQIDNAAG 599 >gi|300312548|ref|YP_003776640.1| filamentous hemagglutinin outer membrane protein [Herbaspirillum seropedicae SmR1] Length = 2685 Score = 121 bits (303), Expect = 6e-28, Method: Compositional matrix adjust. Identities = 173/600 (28%), Positives = 262/600 (43%), Gaps = 126/600 (21%) Query: 64 AFAAPIADPAAPIRFQPQVTQSTGVGGGVPVVNITAPNAAGISLNQYSQFNVDAIGLILN 123 A+A IADP AP QP V + G+P VNI P+AAG+S N YSQF+V+ G ILN Sbjct: 70 AYAQIIADPNAPRAQQPVVVNT---ANGIPQVNIQTPSAAGVSRNTYSQFDVNRAGAILN 126 Query: 124 NSLISGGSTLGGTVTANGNLRGRTASQIINQVTTQGSASRIAGTIEVFGTQADVIIANPN 183 NS + LGG V N NL TA I+N+V + + S + G +EV G +A VIIANP+ Sbjct: 127 NSRSDVQTQLGGWVQRNPNLANGTARVILNEVNSS-NPSMLRGFVEVAGDRAQVIIANPS 185 Query: 184 GINCTACGFINTPR--------VVPGAGIEG---SAGVIDLIGGS-----------IGVN 221 G+ C CGFIN R ++ ++G GV+ + G I Sbjct: 186 GVTCDGCGFINASRSTLTTGTPIINNGSLDGYLVRGGVVSIKGNGLNDSQSSFTDLIARA 245 Query: 222 ASINAGQQINLISGQQLVSEAVAGQGKVDSDYSVATNGSSPNFSGPDPTLQIDATAFGAM 281 A INAG I ++L + G +V++D + T S+ P ID G M Sbjct: 246 AKINAG-----IWSKEL--KVTVGTNQVNADNTTVTAVSA---DPAKPQFGIDVAQLGGM 295 Query: 282 NAGQIKVVATTAGLGVK----------------AGVLNANNGNVTIDANGNVNATNVYGS 325 A +I +V T +G+GV+ AG + N+G++ A+ V AT G Sbjct: 296 YANKIVLVGTESGVGVRNAGAIGAAAGELTLTAAGRIE-NSGSLAATASLGVRATGQGGD 354 Query: 326 -----------QGATIRADGSV-SAGTVTSYGAASFNAGTDVTVGQITAANNAQITGRNI 373 ++ADG + ++ T+++ + A + G I+A Sbjct: 355 IVNSGSVASSAAAVKLQADGGINNSNTISAQSGVTLTAASLQNSGAISA----------- 403 Query: 374 AIGNAVVGGALTVNGSGNVTTSQSGAIVG-DAQFTAGGGFTNTGNWTAGGNLNISANTVG 432 A GN V VN SG V + +I Q A +NTGN A G L ++A+ Sbjct: 404 ATGNVEVDTHGAVNNSGKVQAAGDISISATQPQGNAAADISNTGNIEAAGQLGLTAD--- 460 Query: 433 NALSSQLSGLGSTTVTAGSMVNYGTLYGDNLRVTLSGGLDNTFGSMLAKTAMVANVGSLV 492 G + N G++ ++R + G +N + A G LV Sbjct: 461 -----------------GMLSNDGSITAQSIRAGVQGLRNNGSIGTRSNAAFTLQQG-LV 502 Query: 493 SNLGGTMYVGDPNAAANAAPAGDLALTVSSSGSSFNNLGGLIAASNGLTVNAVN------ 546 G + + DL LT+ ++N G + A N LT++A N Sbjct: 503 QGANGRL-----------SAVSDLNLTMQ---GDYDNAGRVQA--NTLTLSAANIGNRSG 546 Query: 547 AAL---DMSGTATGRLNAGGALAVNAGLLVNSGSWLASGSSVSLSGVNGIVNAGVVQSAG 603 AAL D++ TA RL+ G ++ N L + + S L +S ++ G + +VNA +++ G Sbjct: 547 AALQAADITLTALNRLDNAGEISANRQLNITADSIL---NSATILGKDLVVNARTLENTG 603 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.311 0.127 0.353 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 4,727,921 Number of extensions: 185038 Number of successful extensions: 801 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 736 Number of HSP's successfully gapped: 17 Length of query: 1882 Length of database: 1,619,870 Length adjustment: 99 Effective length of query: 1783 Effective length of database: 1,151,105 Effective search space: 2052420215 Effective search space used: 2052420215 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 138 (57.8 bits)