BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0165.0058.p (1199 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312549|ref|YP_003776641.1| hemolysin activation/secretion ... 149 9e-37 gi|300310625|ref|YP_003774717.1| hemolysin activation translocat... 124 5e-29 gi|300309648|ref|YP_003773740.1| hemolysin activation/secretion ... 67 8e-12 >gi|300312549|ref|YP_003776641.1| hemolysin activation/secretion protein [Herbaspirillum seropedicae SmR1] Length = 584 Score = 149 bits (377), Expect = 9e-37, Method: Compositional matrix adjust. Identities = 146/535 (27%), Positives = 239/535 (44%), Gaps = 55/535 (10%) Query: 691 EQVP-EKGPTFQIHHIDVVGNTVLPDDEVQQITQAFVD-KALGGARINLLLRRFTEAFVK 748 E VP ++ P F +H + + G D + + +A + LG IN ++ A V Sbjct: 79 EAVPAQESPCFAVHRVVLDGPGSARFDWLLDLPEANPGGRCLGVQGINAVMSALQNAIVA 138 Query: 749 HGLITTRAYLGEQNLRSGVLVITFVPGKVESIELNGQALSPSKLDASPDSGGWLTDVGVK 808 G +TTR Q++ SG L +T VPGK+ +I +A P + W Sbjct: 139 RGYVTTRILAQPQDIASGELRLTIVPGKIRAIRFAD--------NADPRATQW------- 183 Query: 809 MALPTAPGELLNLTDLEQGVEQINRMRRNKAELQILPG----SSPGGSVIGLNNKVGDRL 864 ALP +PG+LLNL D+EQG+E + R+ +A++QI P ++PG S + ++ + G + Sbjct: 184 NALPASPGDLLNLRDIEQGLENLKRVPTAEADVQIAPSEGKDAAPGDSDLVISYQQGIPI 243 Query: 865 WFSAGIDNYGSQQTGLNRTRLGVEADNLLGFQEILSFNYS--------GSRETNALVGSA 916 + +D+ G++ TG + V DN L ++ ++S G+R + + V Sbjct: 244 RLTLSVDDSGTKATGKYQGSATVSLDNWLTLNDLFYISFSHDLGGGDPGARGSQSQVVHY 303 Query: 917 SVPFGYNTFSYTASVSEYQSL---IGDTALLYGNTVSNAWGWNRVISRSQTSKTAIDTTL 973 S+P GY ++T S + Y I T L G + +N +R++ R KT + Sbjct: 304 SLPLGYWALAFTNSSNRYHQAVAGINQTYLYSGQSETNELKLSRLVYRDAVRKTTLSFKG 363 Query: 974 SLRRADRDINNLTLDPQKLTVLRVAINTLLRFLVRQQPATLTLEAGVSKGLRAFGANKDS 1033 +R + +++ + Q+ V + R + + TL L +G AF A + Sbjct: 364 FVRSSSNYVDDTEVQVQRRRVAGWEMGLAHREFIGE--GTLDLALAYKRGTGAFDAMA-A 420 Query: 1034 PQIQDSEAHGQFTKL--DVNGAITAALP-SIAAAAFTWRTQVSGQWTQQALFGSEQIFVG 1090 P+ E +F + D+N ALP A + QW + L ++ +G Sbjct: 421 PEEAFGEGTSRFRLMTADLNLNAPFALPMPWGKQAMRYTLGARAQWNRTPLTPQDKFSLG 480 Query: 1091 GMSSVRGFR-ESGISGDRGMYARNEVIWANAPTLSDIHFEPYAFFDAGM-----TELISE 1144 G SVRGF ES + +RG Y RNE+ A L E YA D G TEL+ Sbjct: 481 GRYSVRGFDGESNLLAERGWYVRNEL----AAALGASKQEIYAGLDYGEVGGPSTELLV- 535 Query: 1145 QHTRKLSGIGVGVRMQLQLAKHAWSSEITLGRPVQQPDYLEKRSALLLATLNWSY 1199 R+L+G +G+R L + E+ G P+ QPD + +L WS+ Sbjct: 536 --GRRLAGAVLGLRGALGRTNY----EVFAGVPISQPDRFKTSPLTAGMSLMWSF 584 >gi|300310625|ref|YP_003774717.1| hemolysin activation translocator protein [Herbaspirillum seropedicae SmR1] Length = 581 Score = 124 bits (311), Expect = 5e-29, Method: Compositional matrix adjust. Identities = 122/464 (26%), Positives = 207/464 (44%), Gaps = 48/464 (10%) Query: 714 PDDEVQQITQAFVDKALGGARINLLLRRFTEAFVKHGLITTRAYLGEQNLRSGVLVITFV 773 P +Q + LG I +LRR T ++ G TTR +G+Q+L +G L IT V Sbjct: 110 PFHFIQTYLAGYAGSCLGQQGIETILRRVTGQILERGYSTTRVGIGQQDLSTGQLSITLV 169 Query: 774 PGKVESIELNGQALSPSKLDASPDSGGWLTDVGVKMALPTAPGELLNLTDLEQGVEQINR 833 PG + +I A++ S + A PT G LLNL DLEQG+EQ+ R Sbjct: 170 PGIIHAIRFKDPAITAS----------------YRSAFPTQAGALLNLRDLEQGLEQMKR 213 Query: 834 MRRNKAELQILPGSSPGGSVIGLNNKVGDRLWFSAGIDNYGSQQTGLNRTRLGVEADNLL 893 + ++QILP PG S + L K + +D+ G++ TG + + + D+LL Sbjct: 214 VASQDVDMQILPTDVPGQSDVVLEVKRIKPWKLTLSLDDSGAKSTGKYQAGINLAIDDLL 273 Query: 894 GFQEILSFNYSG-------SRETNALVGSASVPFGYNTFSYTASVSEYQSLIGDTALLYG 946 G ++ + +S +R T S S P GY TF + S S Y I A L+ Sbjct: 274 GINDLFNAGFSSDADRMGQARGTTGNNFSYSAPAGYWTFGVSGSTSRYHQRI---AGLFQ 330 Query: 947 NTVSNAWGWN------RVISRSQTSKTAIDTTLSLRRADRDINNLTLDPQKLTVLRVAIN 1000 VS+ N ++ R K ++ + R + I++ ++ Q+ + Sbjct: 331 TFVSSGHSENLEVRVQKLFQRDAQQKNSLQLRIGKRWSRSYIDDTEIEVQRRNTTFAELG 390 Query: 1001 TLLR--FLVRQQPATLTLEAGVSKGLRAFGANKDSP-QIQDSEAHGQFTKLDVNGAITAA 1057 + R F Q ATL GVS F D+P ++ D + +++ ++ + A Sbjct: 391 WMHRHYFGDVQLDATLANRWGVS----WFNGAADAPDRLSDGPTY-RYSMQTIDATVVAP 445 Query: 1058 LPSIAAAAFTWRTQVSGQWTQQALFGSEQIFVGGMSSVRGFR-ESGISGDRGMYARNEVI 1116 + + T+ + GQ ++ L+ ++Q +G +VRGF E ++ + G++ RN++ Sbjct: 446 F-KLGGQSLTYVGTLRGQHSRSRLYLTDQFAIGSRYTVRGFDGELTLASESGVFLRNDLE 504 Query: 1117 WANAPTLSDIHFEPYAFFDAGMT--ELISEQHTRKLSGIGVGVR 1158 A T + + YA DAG ++ L+G +G+R Sbjct: 505 IALGSTGAAL----YAGLDAGRLYGPSVANLQGDTLAGAAIGLR 544 >gi|300309648|ref|YP_003773740.1| hemolysin activation/secretion protein [Herbaspirillum seropedicae SmR1] Length = 569 Score = 67.0 bits (162), Expect = 8e-12, Method: Compositional matrix adjust. Identities = 118/497 (23%), Positives = 200/497 (40%), Gaps = 52/497 (10%) Query: 700 FQIHHIDVVGNTVLPDDEVQQITQAFVDKALGGARINLLLRRFTEAFVKHGLITTRAYLG 759 F+I V GNT+LP D+VQ F + I L + G + ++ Sbjct: 53 FEIRRFVVAGNTLLPQDQVQAAVAPFSGQGRVYGDIQQALEALENLYRSAGYTAVQVHVP 112 Query: 760 EQNLRSG---VLVITFVPGKVESIELNGQALSPSKLDASPDSGGWLTDVGVKMALPTAPG 816 EQ L SG + V+ V GKV+ E + S + + AS + +K +P Sbjct: 113 EQELSSGEVRIDVVETVVGKVQVSE--NRHFSQANIRASIPA--------LKEGVPP--- 159 Query: 817 ELLNLTDLEQGVEQINRMRRNKAELQILPGSSPGGSVIGLNNKVGDRLWFSAGIDNYGSQ 876 NL + + V+ N + + + S + K D L +DN GS Sbjct: 160 ---NLRRISESVQLANDNPAKQVNVTLSAAQSGDQIDAKIEVKDDDPLRVMLNLDNTGSA 216 Query: 877 QTGLNRTRLGVEADNLLGFQEILSFNYSGSRETNALVGSASVPFGYNTFSY--------- 927 TG RT + ++ NL + + Y+ S ++ + GY Y Sbjct: 217 DTGRWRTGVAIQHSNLFNRDHVATVAYTTSPDSPSGSKVDLYSLGYRLPLYELGDSLDFI 276 Query: 928 --TASVSEYQSLIGDTAL-LYGNTVSNAWGWNRVISRSQTSKTAIDTTLSLRRADRD--I 982 +SVS Q+L ++ L + G A WN +R+ + + ++ D I Sbjct: 277 YGKSSVSSGQTLALNSTLNITGRGEVYALRWNHFFARNGEWSSKLVLGADYKKVDSSCAI 336 Query: 983 NNLTLDPQKL-TVLRVAINTL-LRFLVRQQPATLTLEA--GVSKGLRAFGANKDSPQIQD 1038 N L+ L T L + L L + ++Q ++A GV++ + A G + + +Q Sbjct: 337 NGGLLNGATLNTCLPYSTLPLSLTYSAQRQGVGEVIDANIGVARNM-ATGQARRNDALQV 395 Query: 1039 SEAHGQFTK----LDVNGAITAALPSIAAAAFTWRTQVSG--QWTQQALFGSEQIFVGGM 1092 ++ + FT +D A+ + A W+ +++G Q + AL EQ + G Sbjct: 396 TDRYSLFTNGRQSMDNFIAVRGGASWLKAFQNDWQMRLAGNLQLSPHALPAVEQFGLAGA 455 Query: 1093 SSVRGFRESGISGDRGMYARNEVIWANAPTLSDIH--FEPYAFFDAG------MTELISE 1144 ++VRGF E +S D G+ +E+ + S++ AF DAG +T Sbjct: 456 TAVRGFTERAVSADSGVVLNSELYTPDLLAKSELKGSMRLLAFVDAGRGANNNVTANSQV 515 Query: 1145 QHTRKLSGIGVGVRMQL 1161 Q T L+ IG G+R L Sbjct: 516 QSTLTLASIGAGLRYAL 532 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.130 0.367 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 3,467,242 Number of extensions: 145422 Number of successful extensions: 453 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 440 Number of HSP's successfully gapped: 5 Length of query: 1199 Length of database: 1,619,870 Length adjustment: 96 Effective length of query: 1103 Effective length of database: 1,165,310 Effective search space: 1285336930 Effective search space used: 1285336930 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 136 (57.0 bits)