BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0165.0028.p (310 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312008|ref|YP_003776100.1| RNA polymerase sigma-E factor [... 64 1e-11 gi|300311253|ref|YP_003775345.1| RNA polymerase sigma-E factor (... 55 5e-09 >gi|300312008|ref|YP_003776100.1| RNA polymerase sigma-E factor [Herbaspirillum seropedicae SmR1] Length = 163 Score = 64.3 bits (155), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 56/162 (34%), Positives = 77/162 (47%), Gaps = 17/162 (10%) Query: 22 ALRPELHRYCARLMGSVFDGEDVVQDTLIRALEALPELDEATPLRPWLFRIAHNRALDLL 81 A P L RY L G+ +D+VQDTL RA + L +P+RPWLF I HN L Sbjct: 3 ACLPRLRRYARALTGAREAADDLVQDTLERAWQKLDSWQRGSPMRPWLFSIMHN-----L 57 Query: 82 RSRAVRKAEPIAAASD---VVDAANP--DPVEMLMRQEAVNTAVSRFAELPLVQRSVVIL 136 + A R+A P A D +D A DP +L + A+ LP QR V++L Sbjct: 58 HADARRRASPATVALDEYGEIDQAQVALDPGVLLDLETALQL-------LPPEQREVLLL 110 Query: 137 KDVLDESLVEIAVLLNLTVDAVKGHLARGRARLREINALAGQ 178 + + E+AV L + V L+R R RLR + G+ Sbjct: 111 VVLEEMRYEEVAVTLGIPTGTVMSRLSRARERLRRVMQGQGE 152 >gi|300311253|ref|YP_003775345.1| RNA polymerase sigma-E factor (sigma-24) transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 200 Score = 55.5 bits (132), Expect = 5e-09, Method: Compositional matrix adjust. Identities = 47/165 (28%), Positives = 76/165 (46%), Gaps = 10/165 (6%) Query: 10 DERRAEILAVAGALRPELHRYCARLMGSVFDGEDVVQDTLIRALEALPELDEATPLRPWL 69 D++ E+L V + +L R +RL+ + EDVVQ+ I+A ALP+ + WL Sbjct: 19 DKKAFELLVVK--YQRKLMRLVSRLVRDQAEAEDVVQEAFIKAYRALPQFRGDSAFYTWL 76 Query: 70 FRIAHNRALDLLRS---RAVRKAEPIAAASDVVDAANP-----DPVEMLMRQEAVNTAVS 121 +RI N A + L + RA E A ++ D A+ P ML ++ T Sbjct: 77 YRIGINTAKNYLVTMGRRAPTSTEADAEEAETFDDADHLRDINTPESMLATKQIAQTVNV 136 Query: 122 RFAELPLVQRSVVILKDVLDESLVEIAVLLNLTVDAVKGHLARGR 166 LP R+ + L+++ S EIA +N + V+ + R R Sbjct: 137 AMEALPEELRTAITLREIEGLSYDEIADAMNCPIGTVRSRIFRAR 181 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 956,316 Number of extensions: 38402 Number of successful extensions: 133 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 134 Number of HSP's successfully gapped: 2 Length of query: 310 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 225 Effective length of database: 1,217,395 Effective search space: 273913875 Effective search space used: 273913875 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)