BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0165.0025.p (229 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxido... 72 4e-14 gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogena... 71 8e-14 gi|300312738|ref|YP_003776830.1| short chain dehydrogenase [Herb... 62 3e-11 gi|300310643|ref|YP_003774735.1| cis-diol dehydrogenase [Herbasp... 59 3e-10 gi|300311721|ref|YP_003775813.1| NADH-dependent enoyl-[acyl-carr... 59 5e-10 >gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 264 Score = 72.0 bits (175), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 57/230 (24%), Positives = 102/230 (44%), Gaps = 21/230 (9%) Query: 1 VFLTGRNLAGVQAVATGIGGKAEAAVV-----DALDEQSIEAHLKSVIDRVGRVDVSFNA 55 + L GR+ + AVA + +AE + DA + ++A ++ + +G + + N Sbjct: 46 IVLNGRDAGKLAAVAAQL--RAEGHTIHESSFDATSSEEVKAAVQKIESEIGPIGILVNN 103 Query: 56 VGIRNTTLQGVALSNLDIEQFSLPIATYTRSYFLTSRVAARQMIRQKSGVIMTVTSTPSR 115 GI+ L + I+T S F ++ AR MI + +G I+ + S S Sbjct: 104 AGIQRRN----PLEEFKESDWHDLISTNLNSVFFAAQAVARHMIPRGAGKIINICSVQSE 159 Query: 116 IGIPLMGGVGPAMSAVESLTRGLSAELAPHGIRVIGLRPHGMPESDTIKEVFGLHANAWG 175 +G P + AV+ T+G++ + +GI+V GL P F N Sbjct: 160 LGRPGIAPYTATKGAVKMFTKGMAIDWGKYGIQVNGLGPG----------YFKTEMNMAL 209 Query: 176 ISREQFEELIAGRTHTRRLSSLTEMADMAVFAASDQASGLTGTIFNLSLG 225 ++ +F + + GRT +RR + ++ AVF ASD + + G I + G Sbjct: 210 VNDAKFTDWLVGRTPSRRWGDVEDLVGAAVFLASDASRFVNGHILYVDGG 259 >gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 254 Score = 70.9 bits (172), Expect = 8e-14, Method: Compositional matrix adjust. Identities = 57/207 (27%), Positives = 99/207 (47%), Gaps = 17/207 (8%) Query: 17 GIGGKAEAAVVDALDEQSIEAHLKSVIDRVGRVDVSFNAVGIRNTTLQGVALSNL---DI 73 GI G E ++D D+ +I K+++ ++G +DV FN G VA N+ D Sbjct: 52 GIAG-VETHLLDVTDDAAI----KALVAKIGTIDVLFNCAGY-------VAAGNILECDD 99 Query: 74 EQFSLPIATYTRSYFLTSRVAARQMIRQKSGVIMTVTSTPSRI-GIPLMGGVGPAMSAVE 132 + + ++ F T R M+ +K+G I+ + S S + G+ G + +AV Sbjct: 100 KAWDFSFNLNAKAMFHTIRAVLPGMLAKKAGSIVNIASAASSVKGVANRFAYGASKAAVV 159 Query: 133 SLTRGLSAELAPHGIRVIGLRPHGMPESDTIKEVFGLHANAWGISREQFEELIAGRTHTR 192 LT+ ++A+ GIR + P G ES ++ + A G S E+ GR Sbjct: 160 GLTKSVAADFVAQGIRCNAICP-GTIESPSLNQRISTQAKETGKSEEEVRAAFVGRQPMG 218 Query: 193 RLSSLTEMADMAVFAASDQASGLTGTI 219 R+ E+A +A++ ASD+++ TG+I Sbjct: 219 RIGKAEEVAALALYLASDESNFTTGSI 245 >gi|300312738|ref|YP_003776830.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 261 Score = 62.4 bits (150), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 51/193 (26%), Positives = 87/193 (45%), Gaps = 9/193 (4%) Query: 28 DALDEQSIEAHLKSVIDRV-GRVDVSFNAVGIRNTT--LQGVALSNLDIEQFSLPIATYT 84 D E+ ++A + R GR+DV N GI T ++ VALS+ D +A Sbjct: 62 DVSSEREVDAMFADLAQRWSGRLDVLINNAGIAGPTSPVEEVALSDWD-----QTLAVNL 116 Query: 85 RSYFLTSRVAARQMIRQKSGVIMTVTSTPSRIGIPLMGGVGPAMSAVESLTRGLSAELAP 144 FL +R A + + G I+ ++S R+G L + V LT + EL P Sbjct: 117 TGPFLCTRRAVPLLKKNGGGAIVNISSVAGRLGFALRTPYSASKYGVIGLTETWAIELGP 176 Query: 145 HGIRVIGLRPHGMPESDTIKEVFGLHANAWGISREQFEELIAGRTHTRRLSSLTEMADMA 204 IRV + P G+ E + + A A+GI E+ + + R R++ + ++A+ Sbjct: 177 SHIRVNAVLP-GIVEGARQERIVAAKAAAYGIGHEEMRQRLLSRVSLRKMVTAEDIANQV 235 Query: 205 VFAASDQASGLTG 217 +F S + ++G Sbjct: 236 IFLCSPAGASISG 248 >gi|300310643|ref|YP_003774735.1| cis-diol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 257 Score = 58.9 bits (141), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 62/224 (27%), Positives = 96/224 (42%), Gaps = 30/224 (13%) Query: 15 ATGIGGKAEAAVVDALDEQSIEAHLKSVIDRVGRVDVSFNAVG--IRNTTLQGVALSNLD 72 A GG+ + V D +A + + R GR+D+ N VG IR + +D Sbjct: 48 AEAAGGRGKGLVADLESYAGAQAAMDEAVQRYGRIDILINGVGGAIRMRPYAQYTPTQID 107 Query: 73 IE-QFSLPIATYTRSYFLTSRVAARQMIRQKSGVIMTVTSTPSRIGIPLMGGVGPAMSAV 131 E + SL Y L Q+++Q GVI+ V+S +R GI + A V Sbjct: 108 AEIRRSLMPTLYGCHAVLP------QLLQQGRGVIVNVSSNATR-GIRRV-PYSAAKGGV 159 Query: 132 ESLTRGLSAELAPHGIRVIGLRPHGM----------PESDTIKEVFGLHANAWGISREQF 181 +LT L+ E GIRV+ P G PE D+ +E W ++ Sbjct: 160 NALTAALAMEYGELGIRVVATAPGGTQAPPRRVPRNPEGDSQQE------QMW---MQEA 210 Query: 182 EELIAGRTHTRRLSSLTEMADMAVFAASDQASGLTGTIFNLSLG 225 + + G + +R S+L E +F ASD AS +TG + ++ G Sbjct: 211 VQQVTGSNYFKRYSTLEEQIAPILFLASDDASYITGAVLPVAGG 254 >gi|300311721|ref|YP_003775813.1| NADH-dependent enoyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 255 Score = 58.5 bits (140), Expect = 5e-10, Method: Compositional matrix adjust. Identities = 46/158 (29%), Positives = 76/158 (48%), Gaps = 12/158 (7%) Query: 68 LSNLDIEQFSLPIATYTRSYFLTSRVAARQMIRQKSGVIMTVTSTPSRIGIPLMGGVGPA 127 ++N + FSL + S+ +R+A M + G ++T++ + + G +GP Sbjct: 105 VTNCSSQGFSLAMDVSCHSFIRMARLAEPLM--KNGGSLLTMSYIGAEEVVVNYGVMGPV 162 Query: 128 MSAVESLTRGLSAELAPHGIRVIGLRPHGMPESDTIKEVFGLHANAWGISREQFEELIAG 187 +A+ES R L+ EL P GIRV + P + + GL+ EQF A Sbjct: 163 KAALESAVRYLAMELGPAGIRVNAVSPGPL----ATRAASGLNC------FEQFLTDAAR 212 Query: 188 RTHTRRLSSLTEMADMAVFAASDQASGLTGTIFNLSLG 225 R+ RRL+++ ++ DM F SD AS +TG + G Sbjct: 213 RSPLRRLANIDDVGDMCSFLVSDAASVITGNTLYVDAG 250 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.366 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 687,314 Number of extensions: 27400 Number of successful extensions: 185 Number of sequences better than 1.0e-08: 16 Number of HSP's gapped: 189 Number of HSP's successfully gapped: 16 Length of query: 229 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 147 Effective length of database: 1,231,600 Effective search space: 181045200 Effective search space used: 181045200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)