BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0165.0018.p (514 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313037|ref|YP_003777129.1| short-chain dehydrogenase [Herb... 415 e-117 gi|300312612|ref|YP_003776704.1| 3-ketoacyl-CoA reductase [Herba... 132 7e-32 gi|300313745|ref|YP_003777837.1| short-chain alcohol dehydrogena... 129 5e-31 gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum s... 123 4e-29 gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein... 120 3e-28 >gi|300313037|ref|YP_003777129.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 369 Score = 415 bits (1066), Expect = e-117, Method: Compositional matrix adjust. Identities = 196/279 (70%), Positives = 234/279 (83%), Gaps = 1/279 (0%) Query: 235 SMPPQHQNHQPGDEYLMQPHPQSSPKLLAPGTRLSQKVALITGGDSGIGRAVAIAFAKEG 294 ++P QHQ+HQPG E M+P P S+P PG RL+ KVALITGGDSGIGRAVA+A+A+EG Sbjct: 91 ALPAQHQSHQPGSEQQMEPRPHSAPAG-EPGRRLAGKVALITGGDSGIGRAVALAYAREG 149 Query: 295 ATVVVVYLDEHEDAVETQRLVREAGSECWLVSGDVGSEKFCKEAVQAAIDKCGRLDILVN 354 A + +VYLDE EDA +TQ+ V+ + C L+ GDVGS +FC+ AV+A +D GRLD+LVN Sbjct: 150 ARLAIVYLDEEEDARQTQKDVQALDAPCLLLPGDVGSPEFCRSAVRAVMDAYGRLDVLVN 209 Query: 355 NAAQQFPSSDIEKITEEQLEKTFRTNIFSMFHMVKAALPYLVSGARIINTASVTAYRGSG 414 NAAQQ+P+ IE+I++ QLE TFRTNIFSMF+MVK A+P+L GARIINT SVTAYRGS Sbjct: 210 NAAQQYPAQSIEEISQAQLEHTFRTNIFSMFYMVKEAMPFLTRGARIINTTSVTAYRGSK 269 Query: 415 HLLDYSATKGAIVAFTRSLAQQLVKHEIHVNAVAPGPVWTPLIPASFSAEEVAKFGTNVP 474 HL+DY+ATKGAIVAFTRSL+QQL IHVNAVAPGP+WTPLIPASFSAEEVA FG NVP Sbjct: 270 HLVDYAATKGAIVAFTRSLSQQLASAGIHVNAVAPGPIWTPLIPASFSAEEVASFGKNVP 329 Query: 475 MGRPGQPYEIAPSYVFLATDGADYMTGQVLHPNGGEIIN 513 +GRPGQP E+AP+YVFLAT+GA YMTGQVLHPNGGEI+N Sbjct: 330 LGRPGQPDEVAPAYVFLATEGASYMTGQVLHPNGGEIVN 368 >gi|300312612|ref|YP_003776704.1| 3-ketoacyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 253 Score = 132 bits (332), Expect = 7e-32, Method: Compositional matrix adjust. Identities = 88/247 (35%), Positives = 138/247 (55%), Gaps = 4/247 (1%) Query: 268 LSQKVALITGGDSGIGRAVAIAFAKEGATVVVVYLDEHEDAVETQRLVREAGSECWLVSG 327 L+ KV I+G +GIG A A AFA G+ +VV Y ++A VR AGS+ + Sbjct: 4 LAGKVVFISGASTGIGAAAARAFAARGSHLVVHYNSSEKEAEAVAAEVRAAGSKSITIGA 63 Query: 328 DVGSEKFCKEAVQAAIDKCGRLDILVNNAAQQFPSSDIEKITEEQLEKTFRTNIFSMFHM 387 DV AV A+ + GR+D+L+NNA + +E +T+E + N S+ Sbjct: 64 DVRDTSAINAAVAKAVAQMGRIDVLINNAGALVKRAPLETVTDELFDDIININARSVVAF 123 Query: 388 VKAALPYLVS--GARIINTASVTAYRGSG-HLLDYSATKGAIVAFTRSLAQQLVKHEIHV 444 +A +P + + G IIN SV A G G L Y+A+KG + TR +A++L+ +I V Sbjct: 124 TRAVIPVMRAQGGGNIINVTSVAARHGGGPGALIYAASKGFVSTLTRGMAKELLADKIRV 183 Query: 445 NAVAPGPVWTPLIPASFSAEEVAKFGTNVPMGRPGQPYEIAPSYVFLATDG-ADYMTGQV 503 NAVAPG + TP + E++ F +PMGR G+P E + ++++LA++ + Y+TGQ+ Sbjct: 184 NAVAPGVIMTPFQERFTTPEQLEGFRKTIPMGRIGEPDECSGAFLYLASEQMSGYVTGQI 243 Query: 504 LHPNGGE 510 + NGG+ Sbjct: 244 IDVNGGQ 250 >gi|300313745|ref|YP_003777837.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 129 bits (325), Expect = 5e-31, Method: Compositional matrix adjust. Identities = 95/251 (37%), Positives = 127/251 (50%), Gaps = 17/251 (6%) Query: 267 RLSQKVALITGGDSGIGRAVAIAFAKEGATVVVVYLDEHE-DAVETQRLVREAGSECWLV 325 +L+ K+AL+TGG SGIG A A A +GA V + + E DA G Sbjct: 4 QLNGKIALVTGGTSGIGLATAQELAAQGAQVFITGRRQAELDAAVASIGAAATGIRA--- 60 Query: 326 SGDVGSEKFCKEAVQAAIDK-CGRLDILVNNAA--QQFPSSDIEKITEEQLEKTFRTNIF 382 V SE + V A I K GRLDIL NA P + ITEE ++ F TN+ Sbjct: 61 DASVLSEL---DTVYAQIAKSAGRLDILFANAGGGDMMP---LGAITEEHFDRIFGTNVR 114 Query: 383 SMFHMVKAALPYLVSGARIINTASVTAYRGSGHLLDYSATKGAIVAFTRSLAQQLVKHEI 442 + V+ ALP LV GA +I T+S T+ G+ + YSA+K A+ F RS A L I Sbjct: 115 GVLFTVQKALPLLVDGASVILTSSTTSVLGTANFSVYSASKAAVRNFARSWALDLKDRAI 174 Query: 443 HVNAVAPGPVWTP----LIPASFSAEEVAKFGTNVPMGRPGQPYEIAPSYVFLATDGADY 498 VN V+PGP+ TP L+P + VP+GR G+P EI + FLA+D A + Sbjct: 175 RVNVVSPGPIRTPGLGELVPDDARQGLFDYLASQVPLGRLGEPQEIGKAVAFLASDAASF 234 Query: 499 MTGQVLHPNGG 509 + G L +GG Sbjct: 235 VNGIELFVDGG 245 >gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 123 bits (308), Expect = 4e-29, Method: Compositional matrix adjust. Identities = 85/252 (33%), Positives = 134/252 (53%), Gaps = 11/252 (4%) Query: 268 LSQKVALITGGD--SGIGRAVAIAFAKEGATVVVVYLDEHEDAVETQRLVREAGSECWLV 325 L KVA+ITGG +G+G A A A++GA VVV+ L + + A R+ GS+ + Sbjct: 3 LKDKVAVITGGAGINGLGYATARLMAEQGARVVVLDLAQADPAAAAARI----GSQHLGL 58 Query: 326 SGDVGSEKFCKEAVQAAIDKCGRLDILVNNAAQQFPSSDIEKITEEQLEKTFRTNIFSMF 385 DV + C+ A A + + GR+DILVNNA P +E IT ++ ++ Sbjct: 59 VADVTDKASCETAAAAILQQFGRIDILVNNAGITQPVKTLE-ITGADYDRILDVSLRGTL 117 Query: 386 HMVKAALPYLVS--GARIINTASVTAYRGSGHLL--DYSATKGAIVAFTRSLAQQLVKHE 441 +M +A LP + + II +SV+A RG G L YSA K ++ R++A++ Sbjct: 118 YMSQAVLPAMRAQQNGSIICISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGPEG 177 Query: 442 IHVNAVAPGPVWTPLIPASFSAEEVAKFGTNVPMGRPGQPYEIAPSYVFLATDGADYMTG 501 I VN++ PG + T +I + E+ A+ +P+ R G+ +I + VFL +D + Y TG Sbjct: 178 IRVNSITPGLIGTDIIKGKLTEEKKAEIAETIPLARLGRADDIGGACVFLGSDLSQYCTG 237 Query: 502 QVLHPNGGEIIN 513 L NGG +I+ Sbjct: 238 ITLDVNGGMLIH 249 >gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 120 bits (300), Expect = 3e-28, Method: Compositional matrix adjust. Identities = 85/252 (33%), Positives = 135/252 (53%), Gaps = 11/252 (4%) Query: 268 LSQKVALITGG--DSGIGRAVAIAFAKEGATVVVVYLDEHEDAVETQRLVREAGSECWLV 325 L KVA+ITGG +G+G + A A +GA +V++ L+ E A RL G++ + Sbjct: 3 LKDKVAIITGGAGQNGLGFSTARLMAAQGARIVILDLERAEPAGAAARL----GAQHLGL 58 Query: 326 SGDVGSEKFCKEAVQAAIDKCGRLDILVNNAAQQFPSSDIEKITEEQLEKTFRTNIFSMF 385 DV + C+ A A + + GR+DILVNNA P+ +E IT + ++ ++ Sbjct: 59 VADVTDKAACEAAAAAVLKQFGRIDILVNNAGVTQPAKLLE-ITGKDYDRILDVSLRGTL 117 Query: 386 HMVKAALPYLVS--GARIINTASVTAYRGSGHLL--DYSATKGAIVAFTRSLAQQLVKHE 441 +M +A LP + + I+ +SV+A RG G L YSA K ++ R++A++ Sbjct: 118 YMSQAVLPAMQAQQSGSIVCISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGPQG 177 Query: 442 IHVNAVAPGPVWTPLIPASFSAEEVAKFGTNVPMGRPGQPYEIAPSYVFLATDGADYMTG 501 I VN + PG + T + SAE+ A+ +P+GR G P ++ VFLA+D + Y TG Sbjct: 178 IRVNCITPGLISTDINKDILSAEKKAEIAQTIPLGRLGGPDDVGGVCVFLASDLSQYCTG 237 Query: 502 QVLHPNGGEIIN 513 L NGG +I+ Sbjct: 238 VTLDVNGGMLIH 249 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.133 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,638,017 Number of extensions: 71631 Number of successful extensions: 412 Number of sequences better than 1.0e-08: 49 Number of HSP's gapped: 331 Number of HSP's successfully gapped: 49 Length of query: 514 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 425 Effective length of database: 1,198,455 Effective search space: 509343375 Effective search space used: 509343375 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)