BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0165.0012.p (257 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310595|ref|YP_003774687.1| 3-oxoacyl-(acyl-carrier protein... 112 2e-26 gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein... 111 7e-26 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 110 1e-25 gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogena... 106 2e-24 gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reduc... 102 4e-23 >gi|300310595|ref|YP_003774687.1| 3-oxoacyl-(acyl-carrier protein) reductase [Herbaspirillum seropedicae SmR1] Length = 250 Score = 112 bits (281), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 83/251 (33%), Positives = 140/251 (55%), Gaps = 14/251 (5%) Query: 5 FKGKKLLVVGGTSGMGLETARMVVAQGGSAVLVGHRADKA-EEARQALAAIGSVTVLVAD 63 F+G+ ++ GG G G A ++ QG + V++ +KA EAR+ L ++G+V + D Sbjct: 6 FQGRSAIITGGAQGFGYAVAERLL-QGKAKVVLWDMDEKALAEARRKLESLGNVETVKVD 64 Query: 64 LSSQDGLQKLIDAIGAEHADIDLLVNAAGVF-FPKPFLEHQDADYDQYMKLN-KAFFFIT 121 +SSQ +Q+ I+A ID+LV++AG+ P E+ ++ + + ++ F++ Sbjct: 65 ISSQAEVQQAIEATEKLTQSIDILVHSAGIAGQNNPVAEYSPEEWRRVIDIDLNGAFYVN 124 Query: 122 QKVAAGLIAAGRPGAIVNIGSMWGKQAIAATPS-SAYSMAKAGLHSLTQHLAMELASSGI 180 Q V +IA G IVNI S+ GK+ P+ SAYS AKAG+ +LT+ L E A+ I Sbjct: 125 QVVVQRMIAQNY-GRIVNIASIAGKE---GNPTASAYSAAKAGMIALTKSLGKETATKNI 180 Query: 181 RVNAVSPAVVQTPIYEGFIPKADVHSALQGFNGFHPIGRVGTPQDVAEVVTFLLSEKAAW 240 VNA++PA QT I E +A + L P R +++A +V +L+SE+ ++ Sbjct: 181 AVNAITPAAAQTRILEQ-CTQAHIDYMLSKI----PRARFVKVEELAAMVAWLVSEENSF 235 Query: 241 VTGAIWDVDGG 251 T +++D+ GG Sbjct: 236 TTASVFDLSGG 246 >gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 111 bits (277), Expect = 7e-26, Method: Compositional matrix adjust. Identities = 82/256 (32%), Positives = 136/256 (53%), Gaps = 18/256 (7%) Query: 5 FKGKKLLVVGGT--SGMGLETARMVVAQGGSAVLVGHRADKAEEARQALAAIGSVTV-LV 61 K K ++ GG +G+G TAR++ AQG V++ ++AE A A A +G+ + LV Sbjct: 3 LKDKVAIITGGAGQNGLGFSTARLMAAQGARIVIL--DLERAEPAGAA-ARLGAQHLGLV 59 Query: 62 ADLSSQDGLQKLIDAIGAEHADIDLLVNAAGVFFPKPFLEHQDADYDQYMKLN-KAFFFI 120 AD++ + + A+ + ID+LVN AGV P LE DYD+ + ++ + ++ Sbjct: 60 ADVTDKAACEAAAAAVLKQFGRIDILVNNAGVTQPAKLLEITGKDYDRILDVSLRGTLYM 119 Query: 121 TQKVAAGLIAAGRPGAIVNIGSMWGKQAIAATPSSAYSMAKAGLHSLTQHLAMELASSGI 180 +Q V + A + G+IV I S+ ++ YS AKAG+ L + +A E GI Sbjct: 120 SQAVLPAM-QAQQSGSIVCISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGPQGI 178 Query: 181 RVNAVSPAVVQTPIYEGFI---PKADVHSALQGFNGFHPIGRVGTPQDVAEVVTFLLSEK 237 RVN ++P ++ T I + + KA++ + P+GR+G P DV V FL S+ Sbjct: 179 RVNCITPGLISTDINKDILSAEKKAEIAQTI-------PLGRLGGPDDVGGVCVFLASDL 231 Query: 238 AAWVTGAIWDVDGGVM 253 + + TG DV+GG++ Sbjct: 232 SQYCTGVTLDVNGGML 247 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 110 bits (276), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 82/256 (32%), Positives = 131/256 (51%), Gaps = 11/256 (4%) Query: 3 NQFKGKKLLVVGGTSGMGLETARMVVAQGGSAVLVGHRADKAEEARQALAAIG-SVTVLV 61 N + LV GG G+G ++ + A+G + ++ D A + AL A G L Sbjct: 4 NDSGARVALVTGGAMGIGASISQQLAAKGHTVLVADINLDAASKTADALVAAGHRAAPLQ 63 Query: 62 ADLSSQDGLQKLIDAIGAEHADIDLLVNAAGVFFPKPFLEHQDADYDQYMKLNKAFFFIT 121 DL S + + I ++ D+LVN AGV FL++ ++ Q M +N + Sbjct: 64 MDLGSPESIAAAFAHIEKDYGRCDVLVNNAGVARTYSFLDYPLENWLQTMNVNVTGVLLA 123 Query: 122 QKVAAGLIAAGRPGAIVNIGSMWGKQAIAATPSSAYSMAKAGLHSLTQHLAMELASSGIR 181 + AA L+ + G IVNI S+ G +A A +AY +KA + LT+ +A+ELA GI Sbjct: 124 GQHAARLMVKQQWGRIVNISSISGIRAGAG--RTAYGTSKAAVIGLTRQMAIELAQYGIT 181 Query: 182 VNAVSPAVVQTPIYEGFIPKADVHS--ALQGFNGFHPIGRVGTPQDVAEVVTFLLSEKAA 239 VN+V+P V TP+ + HS + + P+ R GT ++A V +L +E++A Sbjct: 182 VNSVAPGPVDTPMTQAM------HSEQTRESYYRLVPMRRYGTTDEIASAVCYLAAEESA 235 Query: 240 WVTGAIWDVDGGVMAG 255 ++TG + VDGG +AG Sbjct: 236 YITGHVIPVDGGFVAG 251 >gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 106 bits (264), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 83/259 (32%), Positives = 133/259 (51%), Gaps = 16/259 (6%) Query: 1 VSNQFKGKKLLVVGGTSGMGLETARMVVAQGGSAVLVGHRADKAEEARQALAAIGSVTVL 60 ++ +F+ K ++V GG+ G+GL TA++ A+G + G RADK E QA+ IG+ V Sbjct: 3 MAKRFEDKVVVVTGGSEGIGLATAKLFAAEGARVYVSGRRADKLE---QAVREIGNGAVG 59 Query: 61 V-ADLSSQDGLQKLIDAIGAEHADIDLLVNAAGVFFP--KPFLEHQDADYDQYMKLN-KA 116 V D + D L +L + A+H +D+L AG KP + D+D LN + Sbjct: 60 VPGDAAKLDDLDRLYARVQADHGRLDVLFANAGTASTEAKPLGMIGEEDFDGLFNLNVRG 119 Query: 117 FFFITQKVAAGLIAAGRPGAIVNIGSMWGKQAIAATPSSAYSMAKAGLHSLTQHLAMELA 176 F QK A L++ G GAIV GS+ G + S Y+ +KA + S + +L Sbjct: 120 LLFSVQK-ALPLMSQG--GAIVLNGSVAGVKGFPG--QSLYNASKAAVRSFARSWTADLK 174 Query: 177 SSGIRVNAVSPAVVQTPIYEGFIP-KADVHSALQGFNGFHPIGRVGTPQDVAEVVTFLLS 235 GIRVN V+P +T + ++ + + L+ P+GR+G P ++ V FL S Sbjct: 175 ERGIRVNVVAPGGTETRLMRDYLEARPGMEDVLK---QVVPLGRLGEPDEIGRAVLFLAS 231 Query: 236 EKAAWVTGAIWDVDGGVMA 254 +++++ G VDGG +A Sbjct: 232 AESSYIAGVELFVDGGSVA 250 >gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 241 Score = 102 bits (253), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 82/253 (32%), Positives = 124/253 (49%), Gaps = 17/253 (6%) Query: 1 VSNQFKGKKLLVVGGTSGMGLETARMVVAQGGSAVLVGHRADKAEEARQALAAIGSVTVL 60 + + KGK LV GGT G+G AR +VA G + G EE A VT++ Sbjct: 4 IPDLLKGKTALVTGGTQGIGAAVARQLVALGARVIAAGLPPFAHEEF-----AGSEVTLV 58 Query: 61 VADLSSQDGLQKLIDAIGAEHADIDLLVNAAGVFFPKPFLEHQDADYDQYMKLNKAFFFI 120 +++S++ ++ + + +D++VN AG+ K E + ++Q + +N Sbjct: 59 EMNVASEESVRAALAGL----TQLDIVVNCAGII--KRGAELETEVFEQVIAVNLTGTMR 112 Query: 121 TQKVAAGLIAAGRPGAIVNIGSMWGKQAIAATPSSAYSMAKAGLHSLTQHLAMELASSGI 180 A L+ R G IVN SM P YS +K G+ LT+ LA+ A+ GI Sbjct: 113 VCAAARELLRQSR-GCIVNTASMLSFFGGGLVPG--YSASKGGVAQLTKSLAIAYAADGI 169 Query: 181 RVNAVSPAVVQTPIYEGFIPKADVHSALQGFNGFHPIGRVGTPQDVAEVVTFLLSEKAAW 240 RVNAV+P + TP+ + D P+GR G P+DV +VV FL S A++ Sbjct: 170 RVNAVAPGWIATPLTQAL---QDDPVRAGPILARTPLGRWGQPEDVGQVVAFLCSPAASF 226 Query: 241 VTGAIWDVDGGVM 253 +TGAI VDGG + Sbjct: 227 MTGAIVPVDGGYL 239 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 854,670 Number of extensions: 36326 Number of successful extensions: 311 Number of sequences better than 1.0e-08: 48 Number of HSP's gapped: 225 Number of HSP's successfully gapped: 48 Length of query: 257 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 174 Effective length of database: 1,226,865 Effective search space: 213474510 Effective search space used: 213474510 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)