BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0165.0007.p (336 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbasp... 170 2e-43 gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Her... 133 2e-32 gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbasp... 127 1e-30 gi|300313828|ref|YP_003777920.1| aryl-alcohol dehydrogenase [Her... 114 8e-27 gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Her... 102 6e-23 >gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 343 Score = 170 bits (430), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 118/328 (35%), Positives = 175/328 (53%), Gaps = 24/328 (7%) Query: 4 RRLGKSDLSISPIGLGTWAIAGPGWEFGWGAQDDEESLAAMEYAVERGVNWIDTAAVYGL 63 RRLG + +S IGLG ++ +F + +DD ESLA ++ A+E G+N +DTA +YG Sbjct: 18 RRLGSNGPLVSAIGLGCMGMS----DF-YAQRDDAESLATIDRALELGINLLDTADMYGP 72 Query: 64 GHAEEIVGQVLRRVPASRRPLVF--TKGSLVWDPVTKEISHTLAPDSLIRE-IEGSLRRL 120 EE++G+ ++ RR F TK + DP + IR+ +EGSL+RL Sbjct: 73 YTNEELLGRAIK----GRRDKFFIATKFGIRRDPSDPGARGVDGSPAYIRQAVEGSLKRL 128 Query: 121 QVETIDLYQIHW--PAFPAGGRDDGIEAAVSALAKAREQGKIRAIGVSNFDVPQLRRAQS 178 VETIDLY H PA P IE + LA + GKIR IG+S L RA Sbjct: 129 GVETIDLYYQHRIDPATP-------IEVTMGVLADLVQAGKIRYIGLSEASAATLERAHR 181 Query: 179 VAGIACLQPPYSALMREVEKEILPFCDQAGIGVIAYSTLQSGLLTGAMTRARIAQLPDDD 238 V + LQ YS R+ E E+L C GIG +AYS L G LTGA+ R L +DD Sbjct: 182 VHPVTALQSEYSLWTRDPEAEVLAACRTLGIGFVAYSPLGRGFLTGAIQ--RFEDLAEDD 239 Query: 239 WRKTRSADYQEPRLSRNLELVEVMRRVGQRHGLTPAAVAIAWVLRQLAVTGAIVGARRPG 298 +R++ + +Q +RNL+L E ++ + +HG TP+ +A+AWV+ Q I G +R Sbjct: 240 FRRS-NPRFQGENFARNLQLAEKVKEMAGQHGCTPSQLALAWVMAQDPHIVPIPGTKRRR 298 Query: 299 QIDGLMAAAGFRLSADELAEIATLLPDG 326 ++ + G +L+ ++L + + P G Sbjct: 299 YLEDNAGSVGVKLAPEDLQALDAIFPRG 326 >gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 335 Score = 133 bits (334), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 112/352 (31%), Positives = 168/352 (47%), Gaps = 48/352 (13%) Query: 1 MQRRRLGKSDLSISPIGLGTWAIAGPGWEFGWGAQDD-EESLAAMEYAVERGVNWIDTAA 59 M RRLGKS+L +S + LG+ +G Q D +ES + A E GVN+IDTA Sbjct: 1 MHYRRLGKSNLKVSALCLGSMM---------FGDQTDLQESGRIVASAREHGVNFIDTAD 51 Query: 60 VYGLGHAEEIVGQVLRRVPASRRPLVFTK---GSLVWDPVTKEISHTLAPDSLIREIEGS 116 VY G +EEIVG++L R V ++ P S + ++RE E Sbjct: 52 VYTKGKSEEIVGKLL---AGQRHEWVLASKLGNAMSGKPNESNYSRSW----IVRETENI 104 Query: 117 LRRLQVETIDLYQIHWPAFPAGGRDDGIEAAVSALAKAREQGKIRAIGVSNF------DV 170 L RL + +D+ +H +D+ +E AV AL GKIR GVSNF ++ Sbjct: 105 LTRLDTDYLDILYMHRDY-----QDENLEEAVQALGDLIRVGKIRGFGVSNFRGWRIAEI 159 Query: 171 PQLRRAQSVAGIACLQPPYSALMREVEKEILPFCDQAGIGVIAYSTLQSGLLTGAMTRAR 230 +L +V QP Y+ L R+ E EILP C G+GV+ YS + G+LTG + + Sbjct: 160 VRLCEKNNVPLPLVCQPYYNLLNRQPEVEILPACAHYGLGVVPYSPIARGVLTG---KYQ 216 Query: 231 IAQLPDDDWRKTR------SADYQEPRLSRNLELVEVMRRVGQRHGLTPAAVAIAWVLRQ 284 Q P D R R +++E +L + + +++ + G+ A AWVL Sbjct: 217 PGQKPAQDTRAGRGDKRMMETEFRE----ESLLIAQRLQQHCEARGVKLGQFATAWVLAN 272 Query: 285 LAVTGAIVGARRPGQIDGLMAAAGFRLSADELAEIATLLPDG----TGVNVP 332 A++ I G R Q++ AA ++SA+E A + +L+ G G N P Sbjct: 273 SAISSVIAGPRTLAQMEDYYDAAELKISAEEEALVDSLVTPGHASTHGYNDP 324 >gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 349 Score = 127 bits (319), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 117/356 (32%), Positives = 177/356 (49%), Gaps = 56/356 (15%) Query: 1 MQRRRLGKSDLSISPIGLGTWAIAGPGWEFGWGAQDDE-ESLAAMEYAVERGVNWIDTAA 59 MQ R LG++DL +S I LGT WG Q+ E ++ A ++YAVERG+N ID A Sbjct: 1 MQYRPLGRTDLKVSLITLGTMT---------WGEQNSEADAHAQLDYAVERGINLIDVAE 51 Query: 60 VYGL-------GHAEEIVGQVLRRVPASRRPLVFTKGSLVWDPVTKEIS--------HTL 104 +Y + G E +G L++ + L+ TK + P K + + L Sbjct: 52 MYPVPPKAETQGLTERYLGTWLKKSGKRDQLLIATKCT---GPARKPHNPRHVRGGINNL 108 Query: 105 APDSLIREIEGSLRRLQVETIDLYQIHWP-----AFPAGG----RDDG---IEAAVSALA 152 L + GSL RLQ++ +DLYQ+HWP +F G D+ IE ++ L+ Sbjct: 109 DRKGLTEALHGSLERLQLDHVDLYQLHWPDRSVNSFGQLGYQHVEDESTVPIEETLTVLS 168 Query: 153 KAREQGKIRAIGVSN---FDVPQLRRAQSVAG---IACLQPPYSALMREVEKEILPFCDQ 206 + + GKIR IG+SN + V Q RA G I +Q PY+ L R E + F + Sbjct: 169 EFVKAGKIRTIGLSNETAWGVAQFLRAAEQHGLERIVTIQNPYNLLNRSFEINLAEFAHR 228 Query: 207 AGIGVIAYSTLQSGLLTGA-MTRARIA--QLPDDDWRKTRSADYQEPRLSRNLELVEVMR 263 +G++AYS L G+L+G + AR A +L + R +R + Q R + E V + R Sbjct: 229 EQVGLLAYSPLAFGMLSGKYLDGARPAGGRLTLFE-RFSRYTNAQAERAT--AEYVALAR 285 Query: 264 RVGQRHGLTPAAVAIAWVLRQLAVTGAIVGARRPGQIDGLMAAAGFRLSADELAEI 319 +HGL PA +A+A+V + +T I+GA Q+ + +A +L D LA I Sbjct: 286 ----QHGLDPAQMALAYVNSRPFLTSNIIGATTLAQLRSNIDSAELQLGEDVLAAI 337 >gi|300313828|ref|YP_003777920.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 344 Score = 114 bits (286), Expect = 8e-27, Method: Compositional matrix adjust. Identities = 108/338 (31%), Positives = 159/338 (47%), Gaps = 31/338 (9%) Query: 1 MQRRRLGKSDLSISPIGLGTWAIAGPGWEFGWGAQDDEESLAAMEYAVERGVNWIDTAAV 60 M RR G+S L + I LG W FG G + E A + A +RGV D A Sbjct: 14 MPYRRTGRSGLLLPAISLGLWQ------NFG-GVDNFETGRAVLRRAFDRGVTHFDLANN 66 Query: 61 YG--LGHAEEIVGQVLRR--VPASRRPLVFTK-GSLVWDPVTKEISHTLAPDSLIREIEG 115 YG G AEE G+ + + +P ++ +K G +W S A LI E Sbjct: 67 YGPPAGSAEENFGRWMAKDFLPYRDELVISSKAGWQMWPGPYGGPSG--ARKHLIASCEQ 124 Query: 116 SLRRLQVETIDLYQIHWPAFPAGGRDDGIEAAVSALAKAREQGKIRAIGVSNFDVPQLRR 175 SL+R+ ++ +D++ H D +E + ALA QGK +G+S++ R+ Sbjct: 125 SLKRMGLDYVDIFYSH-----RVDADTPLEETMGALAHLHRQGKALYVGISSYSPELTRK 179 Query: 176 AQSV---AGIACL--QPPYSALMREVEKEILPFCDQAGIGVIAYSTLQSGLLTGAMTRAR 230 A ++ G+ L QP YS L R +E +LP ++ G+G I +S L GLLT Sbjct: 180 AAAILKSEGVPLLIHQPNYSMLNRAIEGGLLPALEELGVGCIGFSPLAQGLLTSKY---- 235 Query: 231 IAQLPDDDWRKTRSADYQEPRLSR-NLELVEVMRRVGQRHGLTPAAVAIAWVLRQLAVTG 289 + +P+ R TR+ + LS NL + + + + G T A +AIAWVLR VT Sbjct: 236 LGGVPEGA-RVTRAPSLPQRMLSEDNLARIRALNEIASKRGQTLAQMAIAWVLRDARVTS 294 Query: 290 AIVGARRPGQI-DGLMAAAGFRLSADELAEIATLLPDG 326 A++GAR Q+ D L A + +ELA I DG Sbjct: 295 ALIGARTVEQLDDSLDAVNKLDFTQEELASIDKHATDG 332 >gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 329 Score = 102 bits (253), Expect = 6e-23, Method: Compositional matrix adjust. Identities = 95/316 (30%), Positives = 145/316 (45%), Gaps = 21/316 (6%) Query: 1 MQRRRLGKSDLSISPIGLGTWAIAGPGWEFGWGAQDDEESLAAMEYAVERGVNWIDTAAV 60 M+ RR G++ +S IG G WAI G WG +++ AA+ A++ GV++IDTA V Sbjct: 1 MKSRRFGRTGAQVSEIGFGAWAIGG-----SWGDVSAQDAKAALHAALDAGVSFIDTADV 55 Query: 61 YGLGHAEEIVGQVLRRVPASRRPLVFTKGSLVWDPVTKEISHTLAPDSLIREIEGSLRRL 120 YG G +E+++ QVL+ RP V TK P + A S I+ SL+ L Sbjct: 56 YGDGRSEQLIAQVLKE-RGGERPFVATKAGRRLSPHVASGYNDRAQLSAF--IDRSLKNL 112 Query: 121 QVETIDLYQIHWPAFPAGGRDDGIEAAVSALAKAREQGKIRAIGVSNFDVPQLRRAQSVA 180 V+ +DL Q+H P + + + + AL A GKIR GVS V + +A Sbjct: 113 GVDCLDLVQLHCPPTEVYYQQE-VFGHLDALVAA---GKIRHYGVSVEKVEEALKAIEYP 168 Query: 181 GIACLQPPYSALMREVEKEILPFCDQAGIGVIAYSTLQSGLLTGAMTRARIAQLPDDDWR 240 + +Q ++ + L + I VIA L SGLLTG M+ A+ DD + Sbjct: 169 NVKSVQIIFNIFRQRPISLFLQEAQRRDIAVIARVPLASGLLTGKMS-AQTTFAADDHRQ 227 Query: 241 KTRSAD-------YQEPRLSRNLELVEVMRRVGQRHGLTPAAVAIAWVLRQLAVTGAIVG 293 R + + L VE +R + + G+ A A+ W+L + ++ I G Sbjct: 228 FNRHGEAFDVGETFSGVPYEVALAAVEKIRALVPQ-GVPMAQFALRWILMESGISTVIPG 286 Query: 294 ARRPGQIDGLMAAAGF 309 AR Q AA+ Sbjct: 287 ARNVAQAQSNSAASAL 302 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,169,771 Number of extensions: 52302 Number of successful extensions: 209 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 187 Number of HSP's successfully gapped: 6 Length of query: 336 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 250 Effective length of database: 1,212,660 Effective search space: 303165000 Effective search space used: 303165000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)