BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0161.0008.p (207 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311481|ref|YP_003775573.1| transmembrane protein [Herbaspi... 214 6e-57 >gi|300311481|ref|YP_003775573.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 441 Score = 214 bits (544), Expect = 6e-57, Method: Compositional matrix adjust. Identities = 101/126 (80%), Positives = 112/126 (88%) Query: 1 MIDVMKTAQALHQFIMDHDVQLGVNVRSNGAPWNVTTLLAALTRQGFDRRTDGHLVMQDG 60 M DV++TAQALHQFI DHD QL VNVRS GAPW++ TLLAALTRQGFDRRT+G+LVMQDG Sbjct: 282 MPDVIRTAQALHQFIADHDAQLSVNVRSQGAPWSLATLLAALTRQGFDRRTEGYLVMQDG 341 Query: 61 EGEVLFSMSTNVTGAAETTDRLTLLLDVPRVGPARDGYGAMIACARSLAMRLGGTVVDDS 120 EGEVLF++STN T ETTDRLTLLLDVPRV PARDGYGA++ACARSLA RLGG +VDDS Sbjct: 342 EGEVLFALSTNATPTDETTDRLTLLLDVPRVSPARDGYGALVACARSLAQRLGGVIVDDS 401 Query: 121 DQLLSD 126 DQ LSD Sbjct: 402 DQPLSD 407 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 560,363 Number of extensions: 19858 Number of successful extensions: 69 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 69 Number of HSP's successfully gapped: 1 Length of query: 207 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 126 Effective length of database: 1,236,335 Effective search space: 155778210 Effective search space used: 155778210 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 128 (53.9 bits)