BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0159.0033.p (339 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310445|ref|YP_003774537.1| phospholipase D family protein ... 362 e-101 gi|300309580|ref|YP_003773672.1| phosphatidylserine/phosphatidyl... 80 3e-16 >gi|300310445|ref|YP_003774537.1| phospholipase D family protein [Herbaspirillum seropedicae SmR1] Length = 472 Score = 362 bits (930), Expect = e-101, Method: Compositional matrix adjust. Identities = 188/272 (69%), Positives = 218/272 (80%), Gaps = 7/272 (2%) Query: 57 RLCAAVAVMA-----LAGCGSLPRIVPDMDTQSAKAIQIKGGRGILTPEQSKAVLDKFKD 111 R CA + V+A L+ C SLP IVPDMDTQSA+ IQ+KGGRG+L+ QSKAVL+K + Sbjct: 10 RWCATLLVLAGLSALLSSCSSLPTIVPDMDTQSARTIQMKGGRGVLSEAQSKAVLEKLRA 69 Query: 112 SKQDNLILEKHLAVEQAITGSPLVAGNKVSLLIDGPATYASMFAAIREARDHINMETYIF 171 DN +L++H+A+E AITGSPLV GNKV+LLIDGPATYASM AI+ AR HINMETYI Sbjct: 70 QNADNTLLDRHVAIEGAITGSPLVTGNKVTLLIDGPATYASMQQAIQAARYHINMETYIM 129 Query: 172 EDDDAGKQFADLLIDRQKHGVQVNLMYDSVGTLGTPREFFQPLIDAGIQVLEFNPVNPAQ 231 EDD+ G+QFADLLI Q GVQVNLMYDSVG L TPR FFQP+IDAGIQVLE+NP+NP Q Sbjct: 130 EDDEVGRQFADLLIAMQNKGVQVNLMYDSVGALNTPRTFFQPMIDAGIQVLEYNPLNPTQ 189 Query: 232 ARKGWEVNQRDHRKLLIVDGKTAFVGGINISSVYSSGSF--RKRKPKEEDNPNKVPWRDT 289 RK WEVNQRDHRKLL+VDGKTAFVGGINISSVYSSGSF RK+KP+ ++PWRDT Sbjct: 190 LRKDWEVNQRDHRKLLVVDGKTAFVGGINISSVYSSGSFSTRKKKPRVNAEGEQIPWRDT 249 Query: 290 QVRMDGPVVAEFQKMFIDTWQKQHGPELAKKK 321 QVR+DGPV EFQK+F+DTW KQ GP L + Sbjct: 250 QVRIDGPVALEFQKLFVDTWNKQRGPALGDHR 281 >gi|300309580|ref|YP_003773672.1| phosphatidylserine/phosphatidylglycerophosphate/ cardiolipin synthase [Herbaspirillum seropedicae SmR1] Length = 387 Score = 79.7 bits (195), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 62/179 (34%), Positives = 84/179 (46%), Gaps = 21/179 (11%) Query: 137 GNKVSLLIDGPATYASMFAAIREARDHINMETYIFEDDDAGKQFADLLIDRQKHGVQVNL 196 GN+++LL G Y ++ +AI AR I +ETYIF DD G + L GV V + Sbjct: 9 GNQIALLHSGAGFYPALISAIDAARVEIYLETYIFSLDDTGVLVCEALQRAAGRGVVVYV 68 Query: 197 MYDSVGTLGTPRE----FFQPLIDAGIQVLEFNPVNPAQARKGWEVNQRDHRKLLIVDGK 252 + D LGT RE L AG+Q FNP R+G R HRKL +VD + Sbjct: 69 IND---WLGTGRENTNKLHLALSAAGVQHRSFNP----WFRRG---VARSHRKLCVVDRQ 118 Query: 253 TAFVGGINISSVYSSGSFRKRKPKEEDNPNKVPWRDTQVRMDGPVVAEFQKMFIDTWQK 311 AFVGG+NI+ S ++ P P D V + GP+V E + W + Sbjct: 119 VAFVGGLNINDDMVS-------DRDHMTPLPAPRWDFAVSILGPLVQEIYREVQAQWLR 170 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,034,629 Number of extensions: 41330 Number of successful extensions: 134 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 132 Number of HSP's successfully gapped: 2 Length of query: 339 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 253 Effective length of database: 1,212,660 Effective search space: 306802980 Effective search space used: 306802980 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)