BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0159.0030.p (579 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [... 242 8e-65 gi|300314042|ref|YP_003778134.1| signal transduction protein con... 232 5e-62 gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodies... 229 4e-61 gi|300311453|ref|YP_003775545.1| signal transduction containing ... 203 3e-53 gi|300312026|ref|YP_003776118.1| signal transduction protein con... 183 4e-47 >gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1036 Score = 242 bits (617), Expect = 8e-65, Method: Compositional matrix adjust. Identities = 147/414 (35%), Positives = 222/414 (53%), Gaps = 10/414 (2%) Query: 71 VASLTAPEDLSLLQENMQSRLQGDAGTVQYSYRILRRDGTRHWVEVHGSVCPHEGG--TA 128 + ++ PED ++ + + G R +R+DG+ W+ + + G T Sbjct: 398 IGTIAHPEDEPRIRAELARCMDGQKRE-DVELRCVRKDGSVFWLALSCAPIHDAQGRFTG 456 Query: 129 LVETALDVNKQVQSEWQSRMADRVFESTSEGILITDAEFRILAVNPAFTRITGYRPEEAM 188 ++ D+ + Q E + R++ F+S EG+LITDA +IL VN AFT ITGY EE + Sbjct: 457 FRTSSRDITDRKQIEAELRISAVAFDS-REGMLITDANSKILRVNKAFTEITGYSAEEIV 515 Query: 189 DKRSRMMTGPGAQAEINRGMLERLARDGHWQGEMKDRRKDGSWYPAWLSISAIRDNRGGI 248 K ++ + M E + R G WQGE+ DRRK+G YP WL+ISA+++ + Sbjct: 516 GKHPNLLRSDRHGPAFFQEMRESIRRFGKWQGEIWDRRKNGEVYPEWLTISAVKNKDQQV 575 Query: 249 SNYVGVFTDNTIRKEAETKLAFLADHDSLTGLRNRSSLMRIFAQQIEAAQAAGETLALFF 308 ++YV D + RK AE ++ LA D+LT L NR+ L+ Q I + AL F Sbjct: 576 THYVSTHHDISDRKLAEERIRELAFFDALTRLPNRTLLLDRLKQAIALSLRTKTCAALLF 635 Query: 309 VDLDRFKTVNDTLGHHAGDQLLVAAAERLREQLRPDDVLARFGGDEFVVVLGG-----SP 363 +DLD FKT+NDT+GH GD LL A+RL +R +D +AR GGDEFVVVL G Sbjct: 636 IDLDHFKTLNDTIGHEKGDTLLKQVAQRLLANVRENDTVARVGGDEFVVVLEGLNELQQE 695 Query: 364 PQAMIAAIAQRLINSILQPFTINGHEMFISASIGSAAYPEHGEDAITLLKNADIAMYHAK 423 AI ++++ ++ + ++ E +ASIG+ + L+K AD+AMY AK Sbjct: 696 AATQTRAIGEKILTALGSTYQLDDVEYRTTASIGATVFLGRAASVDELMKQADLAMYKAK 755 Query: 424 DRGKNAFQLFNKEMSNHALEQMLLENSLRHAIERKEFELYYQPQFSAQDGAICG 477 + G+NA + F+ M LE+ LE LR AI+ + L+YQPQ + G + G Sbjct: 756 ETGRNALRFFDPAMQTAVLERATLEAGLRKAIDGNQLLLHYQPQV-VEGGRVTG 808 Score = 94.4 bits (233), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 55/127 (43%), Positives = 75/127 (59%), Gaps = 8/127 (6%) Query: 443 EQMLLENSLRHAIERKEFELY-YQPQFSAQDGAICGXGTGYSSLASLKQYPLDTLKIDRS 501 E +L++N I +K F L Y +FS D GTGYSSL+ LK+ PL+ LKID+S Sbjct: 905 ESLLVDNV--EDIIQKMFALKAYGVEFSLDD-----FGTGYSSLSYLKRLPLNQLKIDQS 957 Query: 502 FVKGIPADADDVAITEAIVAIAHKLHLKVVAEGVESQEQHDFLRAAGCDMVQGYLHSQPL 561 FV+ + D +D +I + IV +A L L V+AEGVE+ Q FL AGC QGY +P+ Sbjct: 958 FVRDVLIDPNDASIAKTIVNLAQSLGLGVIAEGVETDAQRSFLADAGCHAYQGYFFCRPV 1017 Query: 562 TASEIEQ 568 T E+ Sbjct: 1018 TVEAFEK 1024 >gi|300314042|ref|YP_003778134.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 948 Score = 232 bits (592), Expect = 5e-62, Method: Compositional matrix adjust. Identities = 131/346 (37%), Positives = 190/346 (54%), Gaps = 3/346 (0%) Query: 135 DVNKQVQSEWQSRMADRVFESTSEGILITDAE---FRILAVNPAFTRITGYRPEEAMDKR 191 D+ + +E R+ + E+++ ILI A+ + I VNPAF RITGY EA+ K Sbjct: 379 DIKARRLAERALRLRHQAIEASANAILILSAQEPDYTIEYVNPAFARITGYSSTEAVGKS 438 Query: 192 SRMMTGPGAQAEINRGMLERLARDGHWQGEMKDRRKDGSWYPAWLSISAIRDNRGGISNY 251 R + G + E + + +++ KDGS I+ +RD G ++++ Sbjct: 439 IRFLEGRDSDPEGFETVWIAMREQREHNVVLRNYHKDGSELWNDFYIAPVRDESGEVTHF 498 Query: 252 VGVFTDNTIRKEAETKLAFLADHDSLTGLRNRSSLMRIFAQQIEAAQAAGETLALFFVDL 311 V D T K E +L F A D+LTGL NR L Q I A+ + L + FVDL Sbjct: 499 VAALYDITSMKRYEAELEFQASRDTLTGLINRHMLRHHLEQAIGVAERQKQALWVVFVDL 558 Query: 312 DRFKTVNDTLGHHAGDQLLVAAAERLREQLRPDDVLARFGGDEFVVVLGGSPPQAMIAAI 371 DRFK VNDTLGH AGD LL A RLR +R D +AR GGDEF+++L + Sbjct: 559 DRFKFVNDTLGHKAGDTLLRTVAGRLRAAVREADTVARLGGDEFIMILPEQDELTLDTRS 618 Query: 372 AQRLINSILQPFTINGHEMFISASIGSAAYPEHGEDAITLLKNADIAMYHAKDRGKNAFQ 431 QR+++++ P T+ GH +F++ S+G A YP G D TL+K+ADIAMY K G+N FQ Sbjct: 619 LQRIMDAVAAPVTLGGHTLFMTCSVGVACYPNDGTDGETLIKHADIAMYRGKQTGRNNFQ 678 Query: 432 LFNKEMSNHALEQMLLENSLRHAIERKEFELYYQPQFSAQDGAICG 477 + M+ ALE++ +E LR+A++ EF L+YQP+ G + G Sbjct: 679 FYTPAMNEQALERLRIEGDLRNALDHGEFLLHYQPKVDLASGRVIG 724 Score = 118 bits (296), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 55/90 (61%), Positives = 70/90 (77%) Query: 479 GTGYSSLASLKQYPLDTLKIDRSFVKGIPADADDVAITEAIVAIAHKLHLKVVAEGVESQ 538 GTGYSSLA LK++P+D LKIDRSFV+ I DADD AIT +I+++A L L+V+AEGVE+ Sbjct: 850 GTGYSSLAYLKRFPIDVLKIDRSFVRDITIDADDAAITASIISLARHLKLRVIAEGVETD 909 Query: 539 EQHDFLRAAGCDMVQGYLHSQPLTASEIEQ 568 EQ D+LR GCD +QGYL S P+ A + EQ Sbjct: 910 EQLDYLRHYGCDEIQGYLFSPPVPADKFEQ 939 >gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor domains protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 229 bits (585), Expect = 4e-61, Method: Compositional matrix adjust. Identities = 160/458 (34%), Positives = 249/458 (54%), Gaps = 24/458 (5%) Query: 38 VYVLRNDG--TVLHANDKMAAMLGYQRGAELVG--MPVASLTAPEDLSLLQENMQSRLQG 93 VY R+D T+ ++ A+ GY L+ + LT PED +L++ ++ + ++ Sbjct: 21 VYRCRDDAQWTMEFVSEGCLALTGYLPEDLLLNQRLSFLELTHPEDRTLVRNHVDACMR- 79 Query: 94 DAGTVQYSYRILRRDGTRHWVEVHG-SVCPHEGGTALVETAL-DVNKQVQSEWQSRMADR 151 + + YRI+R DG WV G + G +E L DV ++ ++ + A+R Sbjct: 80 ERRRIDIEYRIVRADGAVRWVWERGVGIYNANGQVEAIEGFLQDVTERKEAAQALQEAER 139 Query: 152 ----VFESTSEGILITDAEFRILAVNPAFTRITGYR-PEEAM----DKRSRMMTGPGAQA 202 +FE+ EG+ T + +AVNPA RI GY PE+ + D ++ P + Sbjct: 140 RYRSIFENAIEGVFQTTPDGTYIAVNPALARIYGYHSPEDLIVGLRDISHQLYVEPERRQ 199 Query: 203 EINRGMLERLARDGHWQGEMKDRRKDGSWYPAWLS--ISAIRDNRGGISNYVGVFTDNTI 260 E R M E+ + E + R+DG W+S A+ D G +S Y G T Sbjct: 200 EFMRLMEEQGSVSNF---ESRVYRRDGE--IIWISENARAVYDEAGKLSCYEGTVEAITE 254 Query: 261 RKEAETKLAFLADHDSLTGLRNRSSLMRIFAQQIEAAQAAGETLALFFVDLDRFKTVNDT 320 RK E ++ A HD+LTGL NR+ L + + A+ G A+ FVDLD+FK +ND+ Sbjct: 255 RKAHEAEMRHQATHDALTGLPNRNMLHEHLQRALRVARQKGALTAVAFVDLDQFKFINDS 314 Query: 321 LGHHAGDQLLVAAAERLREQLRPDDVLARFGGDEFVVVL-GGSPPQAMIAAIAQRLINSI 379 LGH GD+LL A+RL+ LR D++AR GGDEFV+VL + +A IA + QR++ ++ Sbjct: 315 LGHQVGDELLKTVAQRLQACLREADMVARQGGDEFVLVLQNQTEGEAGIAEVMQRILAAV 374 Query: 380 LQPFTINGHEMFISASIGSAAYPEHGEDAITLLKNADIAMYHAKDRGKNAFQLFNKEMSN 439 +P+ E ++ASIG + YP G+D +LLK+AD AMY AK++G+N FQ F M + Sbjct: 375 ARPWQAGDREFQVTASIGVSRYPADGKDVESLLKHADSAMYRAKEQGRNNFQFFAPWMDS 434 Query: 440 HALEQMLLENSLRHAIERKEFELYYQPQFSAQDGAICG 477 ++ + +LR A+++ EF+LYYQP+ S QDG + G Sbjct: 435 QVSNRLEMLINLRRALDQDEFKLYYQPKLSLQDGRLIG 472 Score = 107 bits (268), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 51/99 (51%), Positives = 71/99 (71%), Gaps = 1/99 (1%) Query: 479 GTGYSSLASLKQYPLDTLKIDRSFVKGIPADADDVAITEAIVAIAHKLHLKVVAEGVESQ 538 GTGYSSL+ L+++P+D LKID+SFV+ I DAD AI +AI+++AH L+L+V+AEGVE + Sbjct: 597 GTGYSSLSYLRRFPVDALKIDKSFVRDIVRDADSAAIVKAIISLAHILNLRVIAEGVEDE 656 Query: 539 EQHDFLRAAGCDMVQGYLHSQPLTASEIEQHWYLGGAQA 577 EQH FL+ CD VQGY +P+ + + W AQA Sbjct: 657 EQHAFLKENACDEVQGYYFGKPMEVA-VFTAWMSEQAQA 694 >gi|300311453|ref|YP_003775545.1| signal transduction containing PAS/GGDEF/EAL domains protein [Herbaspirillum seropedicae SmR1] Length = 683 Score = 203 bits (517), Expect = 3e-53, Method: Compositional matrix adjust. Identities = 118/264 (44%), Positives = 156/264 (59%), Gaps = 12/264 (4%) Query: 221 EMKDRRKDGSWYPAWLSISAIRDNRGGISNYVGVFTDNTIRKEAETKLAFLADHDSLTGL 280 E++ R+K G + A LS+S +RD G +G D T RK AE + LA +D+LTGL Sbjct: 193 EVRRRKKSGEVFWASLSLSVMRDQNGHPIGLIGYLNDITERKNAEKLIHHLAYYDALTGL 252 Query: 281 RNRSSLMRIFAQQIEAAQAAGETLALFFVDLDRFKTVNDTLGHHAGDQLLVAAAERLREQ 340 NR+ L R Q +E A+ + F+DL+RFK +NDTLGH AGD LLV A RLR Sbjct: 253 PNRTLLTRTVDQALEEARHENLHGCVMFIDLNRFKPINDTLGHVAGDMLLVEVAMRLRRA 312 Query: 341 LRPDDVLARFGGDEFVVVLGG----SPPQAMIAAIAQRLINSILQPFTINGHEMFISASI 396 LR DV+AR G DEF V L G SP A +AQ+L+ PF I+GHE+ + ASI Sbjct: 313 LREQDVVARLGADEFAVALFGIDKDSP-----AYVAQKLLALFDDPFFIDGHELRVGASI 367 Query: 397 GSAAYPEHGEDAITLLKNADIAMYHAKDRGKN---AFQLFNKEMSNHALEQMLLENSLRH 453 G + YPE+ D TLL+ ADIAMY AK G+N + +++EM+ + L+ + +E L H Sbjct: 368 GVSLYPENATDTETLLRLADIAMYRAKQGGENNEGGYACYSEEMNRNTLDLLRIETGLLH 427 Query: 454 AIERKEFELYYQPQFSAQDGAICG 477 A E E LYYQP+ Q AI G Sbjct: 428 AFEYNELLLYYQPKVDMQTLAITG 451 Score = 97.8 bits (242), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 53/101 (52%), Positives = 71/101 (70%), Gaps = 9/101 (8%) Query: 475 ICGXG---------TGYSSLASLKQYPLDTLKIDRSFVKGIPADADDVAITEAIVAIAHK 525 ICG G TGYSSL+ LK++P++TLKIDRSF GIP D +D AI AI+ IA + Sbjct: 563 ICGLGITVSLDDFGTGYSSLSYLKRFPINTLKIDRSFTMGIPDDTNDCAIASAIIGIAKQ 622 Query: 526 LHLKVVAEGVESQEQHDFLRAAGCDMVQGYLHSQPLTASEI 566 L KV+AEGVE ++Q +FLR AGC+ +QGY+ S+P+ A + Sbjct: 623 LRHKVIAEGVEDEKQFNFLREAGCNELQGYIFSRPVPAPDF 663 >gi|300312026|ref|YP_003776118.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1106 Score = 183 bits (464), Expect = 4e-47, Method: Compositional matrix adjust. Identities = 92/216 (42%), Positives = 134/216 (62%) Query: 262 KEAETKLAFLADHDSLTGLRNRSSLMRIFAQQIEAAQAAGETLALFFVDLDRFKTVNDTL 321 + A+ ++ LA +D LTGL NR + + I A+ +A+ +DLDRFK +NDT Sbjct: 660 RRADERIRHLAHYDELTGLPNRFLCTQHVSNAITHAEHRNGMVAVILMDLDRFKNINDTF 719 Query: 322 GHHAGDQLLVAAAERLREQLRPDDVLARFGGDEFVVVLGGSPPQAMIAAIAQRLINSILQ 381 GH G+ +L A+R R LR D+LAR GGDEF+V++ + IAQ+L+ + Sbjct: 720 GHETGEAVLQEIAQRFRNSLRELDILARVGGDEFIVLVDDFDDPVQLGEIAQKLLTEARK 779 Query: 382 PFTINGHEMFISASIGSAAYPEHGEDAITLLKNADIAMYHAKDRGKNAFQLFNKEMSNHA 441 PF I+G E +S SIG A YP G++A TL+KNADIAMY AK GK+ ++ F+ E++ + Sbjct: 780 PFIIDGQEAMLSVSIGIATYPGDGDNAQTLIKNADIAMYRAKHHGKDDYRFFSDEINTNT 839 Query: 442 LEQMLLENSLRHAIERKEFELYYQPQFSAQDGAICG 477 +E++ LE LR AIER EF ++YQP+ +G I G Sbjct: 840 VERIALEAELRRAIERAEFVVHYQPKIELAEGRIVG 875 Score = 98.6 bits (244), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 49/98 (50%), Positives = 69/98 (70%), Gaps = 2/98 (2%) Query: 479 GTGYSSLASLKQYPLDTLKIDRSFVKGIPADADDVAITEAIVAIAHKLHLKVVAEGVESQ 538 GTGYSSLA LK++P++TLKID+SF+ IP + + AI +AI+A++H L+LKVV EGVE++ Sbjct: 1001 GTGYSSLAYLKRFPVNTLKIDKSFINDIPDNPNSSAIVQAIIAMSHALNLKVVTEGVETE 1060 Query: 539 EQHDFLRAAGCDMVQGYLHSQPLTASEIEQHWYLGGAQ 576 +Q LR G D QGY S+P+ ++ Q L G Q Sbjct: 1061 KQLQALREFGSDEYQGYYFSKPVPYAQFVQ--LLAGQQ 1096 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,793,185 Number of extensions: 74107 Number of successful extensions: 371 Number of sequences better than 1.0e-08: 44 Number of HSP's gapped: 318 Number of HSP's successfully gapped: 57 Length of query: 579 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 489 Effective length of database: 1,193,720 Effective search space: 583729080 Effective search space used: 583729080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)