BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0159.0022.p
         (364 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300309387|ref|YP_003773479.1| membrane protein [Herbaspirillu...   272   3e-74
gi|300311429|ref|YP_003775521.1| transmembrane protein [Herbaspi...    62   9e-11

>gi|300309387|ref|YP_003773479.1| membrane protein [Herbaspirillum
           seropedicae SmR1]
          Length = 711

 Score =  272 bits (695), Expect = 3e-74,   Method: Compositional matrix adjust.
 Identities = 187/330 (56%), Positives = 224/330 (67%), Gaps = 21/330 (6%)

Query: 41  RMAAAHLRSGSAEVRHGLRCAVAAGVTYLIVHLLQLPFGYWATMATMLVMQPSVADSWSR 100
           R AA+H  +GSAE  H  RCA AAG TYLIVHLL+LPFGYWATMATMLVMQPS+A+SWSR
Sbjct: 396 RKAASHAATGSAEWHHAWRCAAAAGATYLIVHLLELPFGYWATMATMLVMQPSIAESWSR 455

Query: 101 SMERAIGSIVGGMLAVVLCLFIHSPLALALLVFPMAALAMGLRPVSYGLYSLFLTPVFVL 160
           SMERA+GS+VGG+LAVVLCL +HSPLALALLVFP+  L M L+PVSYGLY+ FLTPVFVL
Sbjct: 456 SMERAVGSVVGGVLAVVLCLVVHSPLALALLVFPLTVLTMALKPVSYGLYATFLTPVFVL 515

Query: 161 VADVATEPQLQLSNALLRAGNNVIGAVVAIVATYLLWPQRRQANLHRNLSHMILTNLAYL 220
           VADV  +P  +L+NA LRAGNNVIGAVVA++A+YL WP+R   +    L  M   NLAYL
Sbjct: 516 VADVGGDPAHELANAALRAGNNVIGAVVAVLASYLFWPRRHPEDPQAQLQRMAQLNLAYL 575

Query: 221 RGALQLH--PHAAQMHAYRREACVVNIESGLLLQRLLREGGVDARQIHNARTCVALARRL 278
             AL     P  A +HA+RR ACV N+E+GLLLQR+ RE G   R     RT VAL+RRL
Sbjct: 576 IEALATPAAPPMATLHAHRRAACVANVEAGLLLQRVARERGWPERDHGRGRTAVALSRRL 635

Query: 279 ACAATHIWLEERASA----SAAERQARVEFDNWLRTMEDLFERRVTAAVPCAALLQQRPL 334
           A AA+HIW   +A++          A     +  R + D              LL+ RP 
Sbjct: 636 AAAASHIWAHPQAASPELLGWLRALAAALSAHQSRALHD--------------LLRHRPA 681

Query: 335 VHDLARADAVETVCLLAAAMYPELS-PAGK 363
              LA+A AVET CLL A +  + + PAGK
Sbjct: 682 PMHLAQAAAVETACLLVATLDADTAMPAGK 711


>gi|300311429|ref|YP_003775521.1| transmembrane protein
           [Herbaspirillum seropedicae SmR1]
          Length = 743

 Score = 61.6 bits (148), Expect = 9e-11,   Method: Compositional matrix adjust.
 Identities = 51/200 (25%), Positives = 89/200 (44%), Gaps = 34/200 (17%)

Query: 25  PSLPQSSLRQDALDALRMAAAHLRSGSAEVRHGLRCAVAAGVTYLIVHLLQLPF---GYW 81
           P L Q   +        M   +LR  S   R  +R A+A      I +  QLP+   GYW
Sbjct: 367 PFLTQQKFK------FNMLLVNLRWQSPIFRFAIRVALAVTTGLWIAN--QLPYMSHGYW 418

Query: 82  ATMATMLVMQPSVADSWSRSMERAIGSIVGGMLAVVLCLFIHSPLALALLVFPMAALAMG 141
             +  +++++P+ + +  R  +R IG+++G ++ +++  F+H+PL    ++F  +  A  
Sbjct: 419 IVLTIVIILKPNFSATKQRMADRLIGTVIGCLITMLILRFVHNPLGQLAVLFAASVAAPA 478

Query: 142 LRPVSYGLYSLFLTPVFVLVADVATEPQLQLSNALLRAG----------NNVIGAVVAIV 191
              + Y              A  A+   L L N L+ AG          + +IG V+A V
Sbjct: 479 FTYIKYR-----------YTAIAASVQILMLLNLLVPAGHQGVIGERLIDTIIGVVIATV 527

Query: 192 ATYLL--WPQRRQANLHRNL 209
            +Y+L  W  R    L RN+
Sbjct: 528 FSYVLPAWEYRDLPKLLRNV 547


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.326    0.135    0.403 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 970,575
Number of extensions: 35351
Number of successful extensions: 213
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 211
Number of HSP's successfully gapped: 2
Length of query: 364
Length of database: 1,619,870
Length adjustment: 86
Effective length of query: 278
Effective length of database: 1,212,660
Effective search space: 337119480
Effective search space used: 337119480
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 131 (55.1 bits)