BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0159.0015.p (517 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311887|ref|YP_003775979.1| two component sensor histidine ... 217 2e-57 gi|300311810|ref|YP_003775902.1| two component sensor histidine ... 124 2e-29 gi|300311948|ref|YP_003776040.1| sensor histidine kinase/respons... 91 2e-19 gi|300310566|ref|YP_003774658.1| Hpt sensor hybrid histidine kin... 91 2e-19 gi|300312677|ref|YP_003776769.1| two component hybrid sensor his... 87 2e-18 >gi|300311887|ref|YP_003775979.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 633 Score = 217 bits (553), Expect = 2e-57, Method: Compositional matrix adjust. Identities = 102/230 (44%), Positives = 154/230 (66%), Gaps = 3/230 (1%) Query: 279 RRKTAELEILNRDLESFGYSVSHDLRSPLRVVGEFARILDDEYAGVLDDEGRRFVSIIRK 338 +R+ L +N++LESF YSVSHDLR+PLR + +A IL+++YA +D++GR + +IR+ Sbjct: 377 KRQALHLHSVNKELESFSYSVSHDLRAPLRAISGYAMILEEDYADAIDEKGREQLQVIRR 436 Query: 339 SSGKMISLVEDLLAFSKMGQQPLSMESVNSDLLVGEIVQDFKSANPQRHIRFDIVPLPAA 398 + KM L+ DLL +K L++E + D LV +++ + NP ++ FD+ PL A Sbjct: 437 NVRKMDELINDLLKLAKSTTGELTLERFSMDDLVRQVIAGLRRENP--NVLFDVPPLEGA 494 Query: 399 LAEGKILRQVWWNLIENAIKYSRTRDISIITIGYK-ESTPPVYFVKDNGVGFDMAYAHKL 457 +A ++ QVW NL+ NA+K+S + + + + E +Y V DNGVGFDM YAHKL Sbjct: 495 VANRGLITQVWENLLSNAVKFSSKSEQPAVRVSMQVEPEEYIYGVHDNGVGFDMRYAHKL 554 Query: 458 FGVFQRLHSSQEFEGTGVGLAIVERIVTKHGGKIWAESTIGVGTTFYFTL 507 FG FQRLH +E+ GTG+GLA+V+RIV +H G++WA+S G G +FYF+L Sbjct: 555 FGTFQRLHRQEEYAGTGIGLALVQRIVIRHTGRVWAQSKPGEGASFYFSL 604 >gi|300311810|ref|YP_003775902.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 535 Score = 124 bits (310), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 106/327 (32%), Positives = 158/327 (48%), Gaps = 49/327 (14%) Query: 232 IFNAKRFDLGF-YAGRLYGLLAASFVLGMLLLENSNLYAKLVSMHEQQR----RKTAELE 286 I NA+R G A R+ G S L L +S + EQ R R+ EL Sbjct: 213 IVNARRLADGLPLAARVKGHDEISL-LDDALHRSSERLQEASQQSEQYRSQLERRARELT 271 Query: 287 ILNRDL-------ESFGYSVSHDLRSPLRVVGEFAR-----------------ILDDEYA 322 LN DL E F YSVSHDLRSPL + F++ + D + A Sbjct: 272 ELNADLLQQTQDNEMFIYSVSHDLRSPLVNLQGFSKEIGHATTDLLNAVQALPVPDADKA 331 Query: 323 ---GVLDDEGRRFVSIIRKSSGKMISLVEDLLAFSKMGQ---QPLSMESVNSDLLVGEIV 376 V+ D+ + + I+ + + +++ +L S+ G+ QP+ + + +++ IV Sbjct: 332 KLRSVVADDIQPALRFIQNAVTRSAGIIDSMLRLSRAGRVEYQPVML---DMNVIAQRIV 388 Query: 377 QDFKSANPQRHIRFDIVP-LPAALAEGKILRQVWWNLIENAIKYSRTRDISIITIGY-KE 434 + + ++ P LP A + + QV NL+ NA+ Y ITI + +E Sbjct: 389 AAMSGSIRDKGAVVEVAPELPPAFGDPTAIEQVLGNLVGNAVNYLDPARPGRITIDHLRE 448 Query: 435 ----STPP---VYFVKDNGVGFDMAYAHKLFGVFQRLHSSQEFEGTGVGLAIVERIVTKH 487 +T P VY V+DNG+G AY HK+F FQRLH + G G+GLA+V+R+V +H Sbjct: 449 EGGAATGPETNVYVVRDNGLGIPPAYMHKMFIAFQRLHGN-AAPGEGIGLALVKRVVERH 507 Query: 488 GGKIWAESTIGVGTTFYFTLAPDLPAP 514 GG+IW EST G G++FY L PAP Sbjct: 508 GGRIWVESTEGQGSSFYVALPARPPAP 534 >gi|300311948|ref|YP_003776040.1| sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1145 Score = 91.3 bits (225), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 78/267 (29%), Positives = 138/267 (51%), Gaps = 21/267 (7%) Query: 263 ENSNLYAKLVSMHEQ-----QRRKTAELEILNRDLESFGYSVSHDLRSPLRVVGEFARIL 317 +NL + + H+Q QR K A E N F +SH+LR+PL + +A+IL Sbjct: 655 RQTNLLLQEIEAHKQTDAELQRAKEAA-ESANLAKSRFVAGMSHELRTPLNSILGYAQIL 713 Query: 318 DDEYAGVLDDEGRRFVSIIRKSSGKMISLVEDLLAFSKM--GQQPLSMESVNSDLLVGEI 375 + + + + +IR+S ++SL++ LL +K+ G+ L+ + + + + +I Sbjct: 714 HVD--ATIPPARKDAIGVIRRSGEHLLSLIDGLLDIAKIEAGKMRLASDELALEAFLEQI 771 Query: 376 VQDFKSANPQR--HIRFDIV-PLPAAL-AEGKILRQVWWNLIENAIKYSRTRDISIITIG 431 V F Q+ H RFD LP + A+ K LRQ+ NL+ NA+K++ D +T+ Sbjct: 772 VGMFAPQAEQKGLHFRFDKSGRLPQIVHADQKRLRQILINLLGNAVKFT---DHGGVTLR 828 Query: 432 YKESTPPVYF-VKDNGVGFDMAYAHKLFGVFQRLHSS--QEFEGTGVGLAIVERIVTKHG 488 + + YF V D G+G ++F F+R ++ +E GTG+GLAI +V G Sbjct: 829 LRYAREIAYFDVIDTGIGIADDDRERIFLPFERSSAANLREDIGTGLGLAISRMLVHIMG 888 Query: 489 GKIWAESTIGVGTTFYFTL-APDLPAP 514 G++ +S +G G++F+ + P++ P Sbjct: 889 GELSVQSQVGQGSSFHLKIYLPEVHVP 915 >gi|300310566|ref|YP_003774658.1| Hpt sensor hybrid histidine kinase [Herbaspirillum seropedicae SmR1] Length = 723 Score = 90.9 bits (224), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 74/259 (28%), Positives = 129/259 (49%), Gaps = 21/259 (8%) Query: 260 LLLENSNLYAKLVSMHEQQRRKTAELEILNRDLESFGYSVSHDLRSPLRVVGEFARILDD 319 LL EN+ KLV Q ++ A+ +L R +F ++SH++R+P+ V A +L Sbjct: 62 LLRENARTAEKLV-----QAKEAADHAMLTRG--AFLSTMSHEIRTPMNAVLGMAGLL-- 112 Query: 320 EYAGVLDDEGRRFVSIIRKSSGKMISLVEDLLAFSKMGQQPLSMESVNSDL--LVGEIVQ 377 + LD + F I S+ ++ +V D+L FSK+ + + V DL +V + Sbjct: 113 -RSTRLDTQQAEFAKAIEDSAHALLGIVNDVLDFSKIDVGKMDIAVVEFDLAKVVEASAE 171 Query: 378 DFKSANPQRHIRF----DIVPLPAALAEGKILRQVWWNLIENAIKYSRTRDI--SIITIG 431 ++ +R D P + + +RQ+ NL+ NA+K++ ++ + + Sbjct: 172 ALSFKAREKRVRLLAHLDSRLPPRLVGDPGRIRQILLNLLGNALKFTHAGEVLLRVQALQ 231 Query: 432 YKESTPPVYF-VKDNGVGFDMAYAHKLFGVFQRLHSS--QEFEGTGVGLAIVERIVTKHG 488 V F V+D+G+G D +LF F + SS +++ GTG+GL+I +R+V G Sbjct: 232 VTSDHCRVRFEVRDSGIGMDEDTLRRLFSPFAQADSSVTRQYGGTGLGLSICKRLVELMG 291 Query: 489 GKIWAESTIGVGTTFYFTL 507 G I ES +G G+ F+F L Sbjct: 292 GSIGVESALGTGSCFWFEL 310 >gi|300312677|ref|YP_003776769.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1160 Score = 87.4 bits (215), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 72/238 (30%), Positives = 115/238 (48%), Gaps = 11/238 (4%) Query: 281 KTAELEILNRDLESFGYSVSHDLRSPLRVVGEFARILDDEYAGVLDDEGRRFVSIIRKSS 340 K ELE N+ F ++SH+LR+PL A++L D G L DE RF I + Sbjct: 464 KATELERANQYKSEFLANMSHELRTPLNSTLILAKLLADNKQGNLSDEQVRFAQTISSAG 523 Query: 341 GKMISLVEDLLAFSKM--GQQPLSMESVNSDLLVGEIVQDFKSANPQRHIRFDIVPLPA- 397 +++L+ D+L SKM G+ + ES L E+ F Q+ + F + P Sbjct: 524 NDLLALINDILDLSKMEAGKLDIVPESFPLTKLADELSASFAITAQQKGLGFKVEVEPGV 583 Query: 398 -ALAEGKILR--QVWWNLIENAIKYSRTRDISIITIGYKESTPPVYF-VKDNGVGFDMAY 453 E I R Q+ NL+ NAIK++ ++S+ Y ++ + F V+D G+G Sbjct: 584 PQRMETDIQRLGQILKNLLSNAIKFTEKGEVSLRI--YPDAGGRIAFAVRDTGIGIGEDQ 641 Query: 454 AHKLFGVFQRLHSS--QEFEGTGVGLAIVERIVTKHGGKIWAESTIGVGTTFYFTLAP 509 +F F++ S +++ GTG+GL+I + GG I S +G G+ F +L P Sbjct: 642 HQFIFEAFRQADGSTHRKYGGTGLGLSISRDLAHLLGGDIRVTSRLGEGSIFTLSLPP 699 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.141 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,501,845 Number of extensions: 61336 Number of successful extensions: 355 Number of sequences better than 1.0e-08: 22 Number of HSP's gapped: 320 Number of HSP's successfully gapped: 22 Length of query: 517 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 428 Effective length of database: 1,198,455 Effective search space: 512938740 Effective search space used: 512938740 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 133 (55.8 bits)