BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0153.0050.p (419 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309819|ref|YP_003773911.1| acriflavin resistance transmemb... 793 0.0 gi|300313244|ref|YP_003777336.1| multidrug efflux system transme... 493 e-140 gi|300313893|ref|YP_003777985.1| cation/multidrug efflux pump pr... 456 e-129 gi|300310690|ref|YP_003774782.1| cation/multidrug efflux pump pr... 455 e-129 gi|300309657|ref|YP_003773749.1| cation/multidrug efflux pump pr... 298 5e-82 >gi|300309819|ref|YP_003773911.1| acriflavin resistance transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1048 Score = 793 bits (2049), Expect = 0.0, Method: Compositional matrix adjust. Identities = 392/411 (95%), Positives = 407/411 (99%) Query: 1 MGKFFIDRPVFAWVLAIFIILAGALAITGLPVSQYPSIAPPTIVVTATYPGATAQTLDDS 60 MGKFFIDRPVFAWVLAIFI+LAGALAITGLPVSQYP+IAPPTIVVTATYPGATAQTLDDS Sbjct: 1 MGKFFIDRPVFAWVLAIFIMLAGALAITGLPVSQYPNIAPPTIVVTATYPGATAQTLDDS 60 Query: 61 VTSLIEQEMNGADGLQYMESQSQANGQVQITVTFSPDTNADLAAVDVQNRLKRVEARLPA 120 VTSLIEQEMNGADGLQY+ESQSQANGQVQIT+TFSP+TN DLAAVDVQNRLKRVEARLPA Sbjct: 61 VTSLIEQEMNGADGLQYIESQSQANGQVQITITFSPETNPDLAAVDVQNRLKRVEARLPA 120 Query: 121 AVTQQGVQVTKSRSNFLLFIGLSSTDGKLDPVALGDYLSRNVLNEVKRVPGVGQAQLFGT 180 AVTQQGVQVTKSRSNFLLFIGLSSTDGKLDPVALGDYLSRNVLNE+KRVPGVGQAQLFGT Sbjct: 121 AVTQQGVQVTKSRSNFLLFIGLSSTDGKLDPVALGDYLSRNVLNEIKRVPGVGQAQLFGT 180 Query: 181 ERAMRIWIDPNKLIGLNLTPADVTAAITAQNALVAGGTLGALPSPTSQQISATVVVNGQL 240 ERAMRIWIDPNKLIGLNLTPADVTAAI AQNALVAGGTLGALPSP +QQI+ATVVVNGQL Sbjct: 181 ERAMRIWIDPNKLIGLNLTPADVTAAIQAQNALVAGGTLGALPSPGTQQIAATVVVNGQL 240 Query: 241 TSPEQFGNIILRANADGSSVRMRDVARIEVGGQSYDTSGRLDGKPSSFIGVQLSPTANAL 300 TSPEQFGNIILRANA+G++VR+RDVAR+EVGGQSYDTSGRLDGKPSSFIGVQLSPTANAL Sbjct: 241 TSPEQFGNIILRANANGATVRLRDVARVEVGGQSYDTSGRLDGKPSSFIGVQLSPTANAL 300 Query: 301 GTATAVHKKMEELSKYFPAGVKYTIPYDTSKFVKISIEEVVKTLIEAIVLVFLVMFLFLQ 360 GTATAVHKKMEEL+KYFP GVKYTIPYDTSKFVKISIEEVVKTLIEAIVLVFLVMFLFLQ Sbjct: 301 GTATAVHKKMEELAKYFPTGVKYTIPYDTSKFVKISIEEVVKTLIEAIVLVFLVMFLFLQ 360 Query: 361 NIRYTIIPTIVVPVALMGTFATLLLFGFSINVLTMFGMVLAIGILVDDAIV 411 NIRYTIIPTIVVPVAL+GTFATLLLFGFSINVLTMFGMVLAIGILVDDAIV Sbjct: 361 NIRYTIIPTIVVPVALLGTFATLLLFGFSINVLTMFGMVLAIGILVDDAIV 411 >gi|300313244|ref|YP_003777336.1| multidrug efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1033 Score = 493 bits (1269), Expect = e-140, Method: Compositional matrix adjust. Identities = 235/411 (57%), Positives = 312/411 (75%) Query: 1 MGKFFIDRPVFAWVLAIFIILAGALAITGLPVSQYPSIAPPTIVVTATYPGATAQTLDDS 60 M +FFIDRP+FAWV+A+FI+L G +A++ LP+++YPS+APPT+ + ATYPGAT QT++D Sbjct: 1 MPRFFIDRPIFAWVVALFIVLFGVIAMSQLPIARYPSVAPPTVSINATYPGATPQTMNDG 60 Query: 61 VTSLIEQEMNGADGLQYMESQSQANGQVQITVTFSPDTNADLAAVDVQNRLKRVEARLPA 120 V SLIE+E++G L Y ES + +G QI+VTF P T+ +LA VDVQN+LK +EARLP Sbjct: 61 VISLIERELSGVKNLLYFESSADTSGTAQISVTFKPGTDPELAQVDVQNKLKAIEARLPQ 120 Query: 121 AVTQQGVQVTKSRSNFLLFIGLSSTDGKLDPVALGDYLSRNVLNEVKRVPGVGQAQLFGT 180 V Q GV V + S+FLL + L+S DG+ D V L DYL+RN++ E++R+ GVG+ QLFG Sbjct: 121 TVRQNGVTVESAASSFLLMVSLTSVDGRYDEVRLNDYLARNIVEELRRMEGVGRVQLFGA 180 Query: 181 ERAMRIWIDPNKLIGLNLTPADVTAAITAQNALVAGGTLGALPSPTSQQISATVVVNGQL 240 E+AMRIW+DP KL LT D++ AI+ QNA +A G LG P+ QQI+ + V GQL Sbjct: 181 EQAMRIWLDPFKLNAYGLTVTDISTAISQQNAQIAPGRLGDAPAVRGQQIAMPLTVQGQL 240 Query: 241 TSPEQFGNIILRANADGSSVRMRDVARIEVGGQSYDTSGRLDGKPSSFIGVQLSPTANAL 300 SP QF I+L+A ADGS V + DVAR+E+G QSY + R +GKP+ VQLSP ANA+ Sbjct: 241 QSPAQFAAIVLKAKADGSRVLLGDVARVELGAQSYAFANRENGKPAGVAAVQLSPGANAV 300 Query: 301 GTATAVHKKMEELSKYFPAGVKYTIPYDTSKFVKISIEEVVKTLIEAIVLVFLVMFLFLQ 360 TA A+ +MEEL P G+ +++PY+T+ FVKISI++VV+TLIEA+VLVFLVM+LFLQ Sbjct: 301 RTAAAIQARMEELRPSLPQGMAFSLPYNTAPFVKISIQKVVQTLIEAMVLVFLVMYLFLQ 360 Query: 361 NIRYTIIPTIVVPVALMGTFATLLLFGFSINVLTMFGMVLAIGILVDDAIV 411 N+RYT+IP IV P+AL+GT A + L G+S+NVLTMFGMVLAIGI+VDDAIV Sbjct: 361 NVRYTVIPAIVAPIALLGTLAVMQLAGYSVNVLTMFGMVLAIGIIVDDAIV 411 >gi|300313893|ref|YP_003777985.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1051 Score = 456 bits (1174), Expect = e-129, Method: Compositional matrix adjust. Identities = 224/413 (54%), Positives = 305/413 (73%), Gaps = 2/413 (0%) Query: 1 MGKFFIDRPVFAWVLAIFIILAGALAITGLPVSQYPSIAPPTIVVTATYPGATAQTLDDS 60 M +FFIDRP+FAWV+AI I+L G ++I L + QYP IAPP + + ATY GA+A+TL+DS Sbjct: 1 MARFFIDRPIFAWVIAIIIMLGGMMSIRSLSLEQYPDIAPPKVNIKATYTGASAKTLEDS 60 Query: 61 VTSLIEQEMNGADGLQYMESQSQANGQVQITVTFSPDTNADLAAVDVQNRLKRVEARLPA 120 VT +IEQ+M G D LQYM + S + G +++TF+ TN D+A + VQN+L++V ARLP Sbjct: 61 VTQVIEQQMKGLDNLQYMSASSSSAGSATVSLTFTAGTNPDVAQMQVQNKLQQVTARLPQ 120 Query: 121 AVTQQGVQVTKSRSNFLLFIGLSSTDGKLDPVALGDYLSRNVLNEVKRVPGVGQAQLFGT 180 AV GV VTK+ ++ L+ + L+S D ++ + +GD++S + +++ RV GVG FGT Sbjct: 121 AVQNNGVTVTKASTDILMVLSLTSDDPRITSIDIGDFISSTLTDQISRVEGVGDVTAFGT 180 Query: 181 ERAMRIWIDPNKLIGLNLTPADVTAAITAQNALVAGGTLGALPSPTSQQISATVVVNGQL 240 AMRIWIDP KL L P+D+T+A+ AQN V+ G +GALP+ QQI+AT+ +L Sbjct: 181 NYAMRIWIDPAKLQKYALMPSDITSALQAQNTEVSAGEIGALPAQPGQQINATITARSKL 240 Query: 241 TSPEQFGNIILRANADGSSVRMRDVARIEVGGQSYDTSGRLDGKPSSFIGVQLSPTANAL 300 T+P++F NII++++ DGS V++ DVAR+E+GG+SY + + +GKPSS + +QL+ ANAL Sbjct: 241 TTPDEFRNIIVKSSVDGSVVKLSDVARVELGGESYQVASQQNGKPSSALAIQLATGANAL 300 Query: 301 GTATAVHKKMEELSKYFPAGVKY--TIPYDTSKFVKISIEEVVKTLIEAIVLVFLVMFLF 358 TA AV KK+ EL YFPA +K I YDT+ FV++S+EEVVKTLIEAIVLV L+M+LF Sbjct: 301 ATADAVKKKLAELEPYFPASMKLKTNIAYDTTPFVRVSLEEVVKTLIEAIVLVVLIMYLF 360 Query: 359 LQNIRYTIIPTIVVPVALMGTFATLLLFGFSINVLTMFGMVLAIGILVDDAIV 411 LQNIR T IP I VPV L+GTF L +FG+SIN LTMFGMVLAIG+LVDDAIV Sbjct: 361 LQNIRATFIPAITVPVVLLGTFGILAMFGYSINTLTMFGMVLAIGLLVDDAIV 413 >gi|300310690|ref|YP_003774782.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1049 Score = 455 bits (1170), Expect = e-129, Method: Compositional matrix adjust. Identities = 231/411 (56%), Positives = 303/411 (73%) Query: 1 MGKFFIDRPVFAWVLAIFIILAGALAITGLPVSQYPSIAPPTIVVTATYPGATAQTLDDS 60 M +FFIDRP+FAWVLAI ++LAG +++ LP++QYP+IAPP I +TA YPGA+A+TL+D+ Sbjct: 1 MARFFIDRPIFAWVLAIIVMLAGIVSVLTLPIAQYPAIAPPAIAITANYPGASAKTLEDT 60 Query: 61 VTSLIEQEMNGADGLQYMESQSQANGQVQITVTFSPDTNADLAAVDVQNRLKRVEARLPA 120 VT +IEQ+M G D L YM S S+++G V IT+TF TN D A V VQN+L LP+ Sbjct: 61 VTQIIEQKMKGLDRLSYMASTSESSGSVTITLTFENGTNPDTAQVQVQNKLSLATPLLPS 120 Query: 121 AVTQQGVQVTKSRSNFLLFIGLSSTDGKLDPVALGDYLSRNVLNEVKRVPGVGQAQLFGT 180 V QQG+ VTKS +NFL + +S DG + L DY++ NV + + RV GVG LFG+ Sbjct: 121 EVQQQGITVTKSATNFLNVLAFTSEDGSMSGSDLSDYVAANVQDAISRVEGVGDTTLFGS 180 Query: 181 ERAMRIWIDPNKLIGLNLTPADVTAAITAQNALVAGGTLGALPSPTSQQISATVVVNGQL 240 + AMRIW+DPNKL LTP DV AI AQNA V+ G LG LP+ +QQI+AT+ +L Sbjct: 181 QYAMRIWLDPNKLNSYGLTPLDVKNAIQAQNAQVSSGQLGGLPAAENQQINATITAQTRL 240 Query: 241 TSPEQFGNIILRANADGSSVRMRDVARIEVGGQSYDTSGRLDGKPSSFIGVQLSPTANAL 300 + +QF NI+LR G+ V++RDVARIE+G +SY + GR +GKP++ + ++L+ ANAL Sbjct: 241 RTADQFQNIVLRTLTGGAQVKLRDVARIELGSESYTSVGRYNGKPAAGLAIKLATGANAL 300 Query: 301 GTATAVHKKMEELSKYFPAGVKYTIPYDTSKFVKISIEEVVKTLIEAIVLVFLVMFLFLQ 360 T A+ K++ EL K+FP G+K PYDT+ FV+ISIEEVV+TLIEA+VLVFLVM+LFLQ Sbjct: 301 DTVKAIDKRVAELEKFFPTGMKVQKPYDTTPFVRISIEEVVRTLIEAVVLVFLVMYLFLQ 360 Query: 361 NIRYTIIPTIVVPVALMGTFATLLLFGFSINVLTMFGMVLAIGILVDDAIV 411 N R T+IPTI VPV L+GTF L +FGF+IN LTMF MVLAIG+LVDDAIV Sbjct: 361 NFRATLIPTIAVPVVLLGTFGVLAVFGFTINTLTMFAMVLAIGLLVDDAIV 411 >gi|300309657|ref|YP_003773749.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1071 Score = 298 bits (763), Expect = 5e-82, Method: Compositional matrix adjust. Identities = 177/404 (43%), Positives = 260/404 (64%), Gaps = 1/404 (0%) Query: 1 MGKFFIDRPVFAWVLAIFIILAGALAITGLPVSQYPSIAPPTIVVTATYPGATAQTLDDS 60 + KFFIDRP+FA VL++ ++L G LA+ LP+S+YP + PP++VV A YPGA + + ++ Sbjct: 3 ISKFFIDRPIFAGVLSVLLLLGGVLAMLQLPISEYPEVVPPSVVVRAQYPGANPKVIAET 62 Query: 61 VTSLIEQEMNGADGLQYMESQSQANGQVQITVTFSPDTNADLAAVDVQNRLKRVEARLPA 120 V S +E+++NG + + YM+SQ+ ++G + ITV F + D A VQNR+ + RLP Sbjct: 63 VASPLEEQINGVENMLYMQSQANSDGNLTITVNFRLGVDPDKAQQLVQNRVSQALPRLPE 122 Query: 121 AVTQQGVQVTKSRSNFLLFIGLSSTDGKLDPVALGDYLSRNVLNEVKRVPGVGQAQLFGT 180 V + GV KS + + L+S D + D L +Y NV + + R+ GVG+ LFG+ Sbjct: 123 DVQRLGVTTLKSSPTLTMVVHLTSPDNRYDTTYLRNYAVLNVKDRLARIQGVGEVGLFGS 182 Query: 181 -ERAMRIWIDPNKLIGLNLTPADVTAAITAQNALVAGGTLGALPSPTSQQISATVVVNGQ 239 AMR+W+DPNK+ LT DV AI QN VA G +GA PS + +V G+ Sbjct: 183 GNYAMRVWLDPNKVAQRGLTATDVVRAIREQNVQVAAGVIGASPSSPETPLQLSVNAQGR 242 Query: 240 LTSPEQFGNIILRANADGSSVRMRDVARIEVGGQSYDTSGRLDGKPSSFIGVQLSPTANA 299 L + +F +IIL+A+ DG++ R+ DVAR+E+ Y LD KP+ I + +P ANA Sbjct: 243 LKTEGEFRDIILKASPDGATTRLGDVARVELAASEYGLRSLLDNKPAVAIPIFQAPGANA 302 Query: 300 LGTATAVHKKMEELSKYFPAGVKYTIPYDTSKFVKISIEEVVKTLIEAIVLVFLVMFLFL 359 L +T V M+ELS FP+ V Y+I YD ++FV+ SIE VV TL+EAI LV +V+ +FL Sbjct: 303 LDVSTQVRAAMKELSADFPSSVSYSIVYDPTQFVRASIEAVVHTLLEAIALVVIVVIIFL 362 Query: 360 QNIRYTIIPTIVVPVALMGTFATLLLFGFSINVLTMFGMVLAIG 403 Q R +IIP + VPV+++GTF+ +L FG++IN L++FGMVLAIG Sbjct: 363 QTWRASIIPLLAVPVSIIGTFSLMLGFGYTINALSLFGMVLAIG 406 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,185,017 Number of extensions: 45871 Number of successful extensions: 239 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 220 Number of HSP's successfully gapped: 11 Length of query: 419 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 331 Effective length of database: 1,203,190 Effective search space: 398255890 Effective search space used: 398255890 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)