BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0153.0049.p (596 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309819|ref|YP_003773911.1| acriflavin resistance transmemb... 1085 0.0 gi|300310690|ref|YP_003774782.1| cation/multidrug efflux pump pr... 647 0.0 gi|300313893|ref|YP_003777985.1| cation/multidrug efflux pump pr... 630 0.0 gi|300313244|ref|YP_003777336.1| multidrug efflux system transme... 625 e-180 gi|300309657|ref|YP_003773749.1| cation/multidrug efflux pump pr... 363 e-101 >gi|300309819|ref|YP_003773911.1| acriflavin resistance transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1048 Score = 1085 bits (2806), Expect = 0.0, Method: Compositional matrix adjust. Identities = 530/575 (92%), Positives = 545/575 (94%) Query: 1 VENVERIMSEEGLSPREATRKAMGQITGALIGITLVLIAVFIPMAFFGGAVGAIYRQFSL 60 VENVERIMSEEGLSP +ATRKAMGQI+GALIGITLVLIAVFIPMAFFGGAVGAIYRQFSL Sbjct: 413 VENVERIMSEEGLSPADATRKAMGQISGALIGITLVLIAVFIPMAFFGGAVGAIYRQFSL 472 Query: 61 SMVASMVFSVLMAFTLTPALCATILLPVEKGHHHEKKGFFGWFNRRFNKTASGYQGVIGK 120 SMVASMVFSVLMAFTLTPALCATIL PVEKGHHHEKKGFFGWFNR+FN TASGYQG+IG Sbjct: 473 SMVASMVFSVLMAFTLTPALCATILKPVEKGHHHEKKGFFGWFNRKFNSTASGYQGIIGG 532 Query: 121 MLTKAGRYMIVYAIIVGCVGLLYVRLPASFLPNEDQGYIITNVQLPAGASTGRTLEVIKT 180 ML KAGRYMIVY +IV CVGLLYVRLPASFLPNEDQGYIITNVQLP GAST RT V+K Sbjct: 533 MLNKAGRYMIVYVLIVACVGLLYVRLPASFLPNEDQGYIITNVQLPPGASTSRTTAVVKQ 592 Query: 181 IENYYLKQPAVEHVVAIAGFSFSGNGQNAGLVFTPLKDWGERDKTQSADAIAGRAFGAFS 240 IE YYLK PAVEH +A+ G+SFSGNGQNA LVFTPLKDW +RDK+QSADAIAG+AFGAFS Sbjct: 593 IEEYYLKDPAVEHAIAVLGYSFSGNGQNAALVFTPLKDWSQRDKSQSADAIAGKAFGAFS 652 Query: 241 QIKDAIVFPLNPPPIPELGNATGFTFRLQDRAGLGHAALVQARNQILGMASQSKVLAGVR 300 QIKDAIVFPLNPPPIPELGNATGFTFRLQDRAGLGH ALVQARNQILGMASQSKVLAGVR Sbjct: 653 QIKDAIVFPLNPPPIPELGNATGFTFRLQDRAGLGHTALVQARNQILGMASQSKVLAGVR 712 Query: 301 PEGLEDAPQLQLDIDRDKANALGVPFSTINSVLSTALGSSYVNDFPNQGRQQRVIVQADA 360 PEGLEDAPQLQLDIDRDKANALGVPFSTINSVLSTALGSSYVNDFPN GRQQRVIVQADA Sbjct: 713 PEGLEDAPQLQLDIDRDKANALGVPFSTINSVLSTALGSSYVNDFPNLGRQQRVIVQADA 772 Query: 361 KRRLQPEDLAGLYARNSGGTMVPFSSFTTSKWINGPVQLIRYNGYPAMKIAGGAAPGRST 420 KRRLQPEDL LYARNS GTMVPFS+F T+KWINGPVQLIRYNGYPAMKIAGGAAPGRST Sbjct: 773 KRRLQPEDLTNLYARNSSGTMVPFSAFVTAKWINGPVQLIRYNGYPAMKIAGGAAPGRST 832 Query: 421 GEAMAEMEGFVAKLPAGFGFEWTGQSLEEKTSGSQAPALYALSLIAVFLVLAALYESTSI 480 GEAMAEME FVAKLP GFGFEWTGQSLEEKTSG+QAPALYALSLIAVFLVLAALYESTSI Sbjct: 833 GEAMAEMESFVAKLPPGFGFEWTGQSLEEKTSGNQAPALYALSLIAVFLVLAALYESTSI 892 Query: 481 PLAVMLVVPLGILGALLGVTLRGMPNDVYFKVGMIAVVGLSAKNAILIIEFAKDLQAQGM 540 PLAVMLVVPLGILGALLGVTLR MPNDVYFKVGMIAVVGLSAKNAILIIEFAKDLQAQG Sbjct: 893 PLAVMLVVPLGILGALLGVTLRAMPNDVYFKVGMIAVVGLSAKNAILIIEFAKDLQAQGK 952 Query: 541 GLIEATLEAVHLRFRPIIMTSLAFILGVLPLGAAS 575 GLIEATLEAVHLRFRPIIMTSLAFILGVLPL AS Sbjct: 953 GLIEATLEAVHLRFRPIIMTSLAFILGVLPLAIAS 987 >gi|300310690|ref|YP_003774782.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1049 Score = 647 bits (1670), Expect = 0.0, Method: Compositional matrix adjust. Identities = 321/575 (55%), Positives = 420/575 (73%), Gaps = 4/575 (0%) Query: 1 VENVERIMSEEGLSPREATRKAMGQITGALIGITLVLIAVFIPMAFFGGAVGAIYRQFSL 60 VENVER+MSEEGL P++ATRK+MGQI+GAL+G+ LVL AVF+PMAFF G+ G IYRQFS+ Sbjct: 413 VENVERVMSEEGLPPKQATRKSMGQISGALVGVALVLAAVFVPMAFFSGSTGVIYRQFSI 472 Query: 61 SMVASMVFSVLMAFTLTPALCATILLPVEKGHHHEKKGFFGWFNRRFNKTASGYQGVIGK 120 ++V++M SVL+A LTPALCAT+L P KGH GFFGWFNR+F+++ YQG++ Sbjct: 473 TIVSAMTLSVLVALVLTPALCATLLKPAAKGHALATTGFFGWFNRQFDRSNIRYQGIVRH 532 Query: 121 MLTKAGRYMIVYAIIVGCVGLLYVRLPASFLPNEDQGYIITNVQLPAGASTGRTLEVIKT 180 ML + RYM YA+++ V +++LP FLP+EDQG + +QLP+GA+ RT EVI+ Sbjct: 533 MLGRGWRYMAAYAVLLALVVAGFMKLPVGFLPDEDQGTLFAIMQLPSGATKARTDEVIRQ 592 Query: 181 IENYYL--KQPAVEHVVAIAGFSFSGNGQNAGLVFTPLKDWGERD-KTQSADAIAGRAFG 237 +E ++L ++ AV V A++GFSF+G+GQN G F LK W ER S +A +A Sbjct: 593 VEQHFLVDQKEAVSGVFAVSGFSFAGSGQNMGFAFIKLKPWDERKGPGLSVTDVAAKAGA 652 Query: 238 AFSQIKDAIVFPLNPPPIPELGNATGFTFRLQDRAGLGHAALVQARNQILGMASQSKVLA 297 AF+ I+DA VF PP + ELGNATGF L+D A LGHAAL+QARNQ+LG Q K L Sbjct: 653 AFANIRDARVFAFAPPAVSELGNATGFDLMLKDEANLGHAALMQARNQLLGELMQDKRLV 712 Query: 298 GVRPEGLEDAPQLQLDIDRDKANALGVPFSTINSVLSTALGSSYVNDFPNQGRQQRVIVQ 357 VRP GLED P+ +L +D DKA ALGV S IN ++TA GSSYVNDF ++GR ++V++Q Sbjct: 713 AVRPNGLEDTPEFRLSVDTDKAGALGVAMSDINDTIATAWGSSYVNDFIDKGRVKKVMLQ 772 Query: 358 ADAKRRLQPEDLAGLYARNSGGTMVPFSSFTTSKWINGPVQLIRYNGYPAMKIAGGAAPG 417 DA+ R+ P D+ Y RNS GTMVPFS+F+T+ W +G +L RYNG P+M+I G A PG Sbjct: 773 GDAQYRMLPSDVDRWYVRNSAGTMVPFSAFSTASWTSGSPRLERYNGVPSMEILGMALPG 832 Query: 418 -RSTGEAMAEMEGFVAKLPAGFGFEWTGQSLEEKTSGSQAPALYALSLIAVFLVLAALYE 476 S+GEAMA +E +AK+P G G+EWTG SL+EK S + LY +S++ VFL LAALYE Sbjct: 833 AASSGEAMAIVEAALAKMPPGIGYEWTGLSLQEKASSGKTGILYGVSILIVFLALAALYE 892 Query: 477 STSIPLAVMLVVPLGILGALLGVTLRGMPNDVYFKVGMIAVVGLSAKNAILIIEFAKDLQ 536 S ++P +V+LVVPLG+ GALLG L NDVYF+VG++ +GL+ KNAILI+EFAKDL Sbjct: 893 SWAVPFSVILVVPLGVFGALLGAMLTWKMNDVYFQVGLLTTIGLACKNAILIVEFAKDLY 952 Query: 537 AQGMGLIEATLEAVHLRFRPIIMTSLAFILGVLPL 571 G L+EAT+EAV +R RPI+MTSLAFILGVLP+ Sbjct: 953 EHGKSLVEATMEAVRMRLRPILMTSLAFILGVLPM 987 >gi|300313893|ref|YP_003777985.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1051 Score = 630 bits (1625), Expect = 0.0, Method: Compositional matrix adjust. Identities = 312/577 (54%), Positives = 414/577 (71%), Gaps = 3/577 (0%) Query: 1 VENVERIMSEEGLSPREATRKAMGQITGALIGITLVLIAVFIPMAFFGGAVGAIYRQFSL 60 VENVER+MSEE LSP+EATRK+M +ITGAL+GI +VL AVFIPMAFFGG+ G IYRQFS+ Sbjct: 415 VENVERLMSEEKLSPKEATRKSMQEITGALVGIAMVLSAVFIPMAFFGGSTGVIYRQFSI 474 Query: 61 SMVASMVFSVLMAFTLTPALCATILLPVEKGHHHEKKGFFGWFNRRFNKTASGYQGVIGK 120 ++V++M SV++A TLTPALCAT+L P GH + + FF WFNR F+++A+ YQG + Sbjct: 475 TIVSAMALSVMVALTLTPALCATLLKPHAGGHVQQGR-FFTWFNRSFDRSATRYQGGVAG 533 Query: 121 MLTKAGRYMIVYAIIVGCVGLLYVRLPASFLPNEDQGYIITNVQLPAGASTGRTLEVIKT 180 +L + R +++YA+I + +L++RLP SFLP EDQG ++ +QLP GA+ +T V+KT Sbjct: 534 LLKRGKRGLLMYALIAVAMAVLFMRLPTSFLPEEDQGVLMAQIQLPTGATAEQTATVVKT 593 Query: 181 IENYYLKQP-AVEHVVAIAGFSFSGNGQNAGLVFTPLKDWGERDKT-QSADAIAGRAFGA 238 ++ Y++ +P +VE V+AI G GN QN F LKDW +R QSA + RA Sbjct: 594 VQEYFMNKPESVESVLAIVGAGVGGNSQNGARAFIRLKDWEQRTGAGQSASELVRRANAD 653 Query: 239 FSQIKDAIVFPLNPPPIPELGNATGFTFRLQDRAGLGHAALVQARNQILGMASQSKVLAG 298 S+I+ A VF +NPP + LG ++GF L+D G+GH AL+QAR+Q L +A + +LA Sbjct: 654 LSKIRGARVFLMNPPAVRGLGQSSGFDLELKDVGGVGHDALLQARDQFLQLARSNALLAN 713 Query: 299 VRPEGLEDAPQLQLDIDRDKANALGVPFSTINSVLSTALGSSYVNDFPNQGRQQRVIVQA 358 VR GL+D PQL++ ID KA+AL + S IN+ L+TA+G +YVNDF + GR ++V VQ Sbjct: 714 VRTTGLDDTPQLRVAIDDRKADALSIATSDINATLATAMGGTYVNDFLSNGRVKKVYVQG 773 Query: 359 DAKRRLQPEDLAGLYARNSGGTMVPFSSFTTSKWINGPVQLIRYNGYPAMKIAGGAAPGR 418 D+ R+Q +DL Y RN+ MVPF++F +S+W GP L RYNG + +I G A G Sbjct: 774 DSAFRMQADDLNRWYVRNAASQMVPFAAFMSSQWTYGPSLLERYNGVSSFEIVGEPAAGV 833 Query: 419 STGEAMAEMEGFVAKLPAGFGFEWTGQSLEEKTSGSQAPALYALSLIAVFLVLAALYEST 478 S+G AMAE+E VA+LPAG GFEWTG S +E+ SG+QAP LYA+S++ VFL LAALYES Sbjct: 834 SSGTAMAEVEKLVAQLPAGIGFEWTGSSYQERLSGNQAPMLYAISILFVFLCLAALYESW 893 Query: 479 SIPLAVMLVVPLGILGALLGVTLRGMPNDVYFKVGMIAVVGLSAKNAILIIEFAKDLQAQ 538 S+P AV+L VPLGI+G +L + GM NDVYF+VG++ VGLSAKNAILI+EFAK L Sbjct: 894 SVPFAVILAVPLGIIGTVLLTGIFGMSNDVYFQVGLLTTVGLSAKNAILIVEFAKQLHES 953 Query: 539 GMGLIEATLEAVHLRFRPIIMTSLAFILGVLPLGAAS 575 G L EATLEAV LR RPI+MTSLAF+ GVLPL AS Sbjct: 954 GKSLWEATLEAVQLRLRPILMTSLAFMFGVLPLAVAS 990 >gi|300313244|ref|YP_003777336.1| multidrug efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1033 Score = 625 bits (1613), Expect = e-180, Method: Compositional matrix adjust. Identities = 324/575 (56%), Positives = 414/575 (72%), Gaps = 2/575 (0%) Query: 1 VENVERIMSEEGLSPREATRKAMGQITGALIGITLVLIAVFIPMAFFGGAVGAIYRQFSL 60 VENVERIM+ EGL PREAT KAMG+ITGA+IGITLVL AVFIPMA GG+VG IYRQFS+ Sbjct: 413 VENVERIMAHEGLGPREATIKAMGEITGAIIGITLVLTAVFIPMALAGGSVGVIYRQFSV 472 Query: 61 SMVASMVFSVLMAFTLTPALCATILLPVEKGHHHEKKGFFGWFNRRFNKTASGYQGVIGK 120 +M S++FS +A +LTPALCAT+L PV GHH +K+GFFG FNR FN+ + Y+ +G+ Sbjct: 473 AMAVSILFSAFLALSLTPALCATLLRPVPAGHH-DKRGFFGAFNRAFNRLTARYESALGR 531 Query: 121 MLTKAGRYMIVYAIIVGCVGLLYVRLPASFLPNEDQGYIITNVQLPAGASTGRTLEVIKT 180 ++ + R M+VYA + G + + LP++FLP EDQGY +T+ QLPA A+T RT + Sbjct: 532 LIARGLRMMLVYAALSGLLVVGMQALPSAFLPEEDQGYFMTSFQLPADATTERTARAVDQ 591 Query: 181 IENYYLKQPAVEHVVAIAGFSFSGNGQNAGLVFTPLKDWGERDKTQSADAIAGRAFGAFS 240 E Y + + +++ GF FSG+G NA + FT LKDW ER + SA S Sbjct: 592 FERYLKGREDIGANLSVLGFGFSGSGPNAAMAFTELKDWKERKGSTSAAEAMAAQAAMES 651 Query: 241 QIKDAIVFPLNPPPIPELGNATGFTFRLQDRAGLGHAALVQARNQILGMASQSKVLAGVR 300 + D +V L PP I ELGN++GF RLQDRA G+AAL A Q+L +A++S+ L+GV Sbjct: 652 GV-DGMVMSLLPPAIEELGNSSGFAMRLQDRANQGYAALKAAEAQLLALAAKSRKLSGVY 710 Query: 301 PEGLEDAPQLQLDIDRDKANALGVPFSTINSVLSTALGSSYVNDFPNQGRQQRVIVQADA 360 +GL +QL+IDR KA ALGV FS+I+ LS A+GS YVNDFPN GR Q+VI+QA A Sbjct: 711 SDGLPAGASVQLEIDRHKAAALGVSFSSISDTLSAAMGSFYVNDFPNAGRMQQVILQAQA 770 Query: 361 KRRLQPEDLAGLYARNSGGTMVPFSSFTTSKWINGPVQLIRYNGYPAMKIAGGAAPGRST 420 R+Q ED+ LY RN G MVP + + W P QL RY G+PA++I+G AAPG ++ Sbjct: 771 SARMQLEDVMKLYVRNQSGGMVPLAELVKASWREAPTQLQRYQGFPAVRISGSAAPGVAS 830 Query: 421 GEAMAEMEGFVAKLPAGFGFEWTGQSLEEKTSGSQAPALYALSLIAVFLVLAALYESTSI 480 GEAMAEME +LP GF WTGQSL+E+ S +QAP L ALS++ VFLVLAALYES SI Sbjct: 831 GEAMAEMERLAQQLPPGFAVAWTGQSLQERLSAAQAPMLMALSMLVVFLVLAALYESWSI 890 Query: 481 PLAVMLVVPLGILGALLGVTLRGMPNDVYFKVGMIAVVGLSAKNAILIIEFAKDLQAQGM 540 PL+VMLVVPLG++GA+ V LR MPNDV+FKVGMI V+GLSAKNAILI+EFA+ L+++GM Sbjct: 891 PLSVMLVVPLGLVGAVAAVILRDMPNDVFFKVGMITVIGLSAKNAILIVEFARQLRSEGM 950 Query: 541 GLIEATLEAVHLRFRPIIMTSLAFILGVLPLGAAS 575 GL+EA ++A LR RPI+MTSLAF LGV+PL AS Sbjct: 951 GLVEAAIKASVLRLRPILMTSLAFALGVVPLMLAS 985 >gi|300309657|ref|YP_003773749.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1071 Score = 363 bits (931), Expect = e-101, Method: Compositional matrix adjust. Identities = 237/595 (39%), Positives = 349/595 (58%), Gaps = 32/595 (5%) Query: 2 ENVERIMSEEGLSPREATRKAMGQITGALIGITLVLIAVFIPMAFFGGAVGAIYRQFSLS 61 ENVER + GLSPR+AT +AM +++G +I I L L+AVF+P+AF G G Y+QF+++ Sbjct: 417 ENVERNIGA-GLSPRDATYRAMQEVSGPIIAIALTLVAVFVPLAFMTGLTGQFYKQFAMT 475 Query: 62 MVASMVFSVLMAFTLTPALCATILLPVEKGHHHEKKGFFGWFNRRFNKTASGY------- 114 + S V S AF + L + + KGH + W R+ ++ G+ Sbjct: 476 IAISTVIS---AFN-SLTLSPALAALLLKGHGDKPD----WLTRQMDRFLGGFFIRFNRF 527 Query: 115 ---------QGVIGKMLTKAGRYMIVYAIIVGC-VGLLYVRLPASFLPNEDQGYIITNVQ 164 GV G ++ + G M VY +++ +G+ Y+ +P F+P +D+ Y++ Q Sbjct: 528 FNRASDNYGHGVTG-VIKRKGATMGVYVLLLAATLGISYI-VPGGFVPGQDKQYLVAFAQ 585 Query: 165 LPAGASTGRTLEVIKTIENYYLKQPAVEHVVAIAGFSFSG--NGQNAGLVFTPLKDWGER 222 LP GAS R+ EV++ + + LK+P V +A G S +G N +AG+VF LK + ER Sbjct: 586 LPNGASIDRSEEVMRKMSDIMLKEPGVASAIAFPGLSINGFTNSSSAGIVFVGLKPFEER 645 Query: 223 -DKTQSADAIAGRAFGAFSQIKDAIVFPLNPPPIPELGNATGFTFRLQDRAGLGHAALVQ 281 K S +AIA F IK+A PPP+ LG GF +++DR LG+A L + Sbjct: 646 KSKDLSGNAIAASLNKKFGSIKEAFTAVFPPPPVMGLGTLGGFKMQIEDRDALGYAELDK 705 Query: 282 ARNQILGMASQSKVLAGVRPEGLEDAPQLQLDIDRDKANALGVPFSTINSVLSTALGSSY 341 A + A++ L + + PQL +++DR KA LG+P + + + + LGS Y Sbjct: 706 AAQAFMKAAAKEPALGPMFSSYQINVPQLDVELDRVKAKQLGIPVTDVFNTMQIYLGSLY 765 Query: 342 VNDFPNQGRQQRVIVQADAKRRLQPEDLAGLYARNSGGTMVPFSSFTTSKWINGPVQLIR 401 VNDF GR +V QADA R + ED+A L RN G MVP SS K GP ++R Sbjct: 766 VNDFNRFGRVYQVRAQADAPFRAKAEDIAQLKTRNDAGEMVPLSSVVKVKQTFGPEMVVR 825 Query: 402 YNGYPAMKIAGGAAPGRSTGEAMAEMEGFVA-KLPAGFGFEWTGQSLEEKTSGSQAPALY 460 YNGY A I GG APG S+ +A A E A LP G FEWT + ++ +G+ ++ Sbjct: 826 YNGYTAADINGGPAPGYSSDQAQAAAERVAAATLPRGIKFEWTDLTYQKILAGNAGVWVF 885 Query: 461 ALSLIAVFLVLAALYESTSIPLAVMLVVPLGILGALLGVTLRGMPNDVYFKVGMIAVVGL 520 +S++ VFLVLAALYES ++PLAV+L+VP+ IL AL GV L N+++ ++G++ +VGL Sbjct: 886 PISVLLVFLVLAALYESLTLPLAVILIVPMSILAALTGVWLTRGDNNIFTQIGLMVLVGL 945 Query: 521 SAKNAILIIEFAKDLQAQGMGLIEATLEAVHLRFRPIIMTSLAFILGVLPLGAAS 575 SAKNAILI+EFA++L+ QG ++A +EA LR RPI+MTS+AFI+GV+PL +S Sbjct: 946 SAKNAILIVEFARELEMQGRTAVQAAIEASRLRLRPILMTSIAFIMGVVPLVTSS 1000 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.138 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,878,065 Number of extensions: 84530 Number of successful extensions: 359 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 329 Number of HSP's successfully gapped: 13 Length of query: 596 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 505 Effective length of database: 1,188,985 Effective search space: 600437425 Effective search space used: 600437425 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)