BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0153.0045.p (393 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 400 e-112 gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipo... 230 1e-61 gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipo... 222 4e-59 gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipo... 216 2e-57 gi|300310854|ref|YP_003774946.1| NodT family protein [Herbaspiri... 141 8e-35 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 400 bits (1027), Expect = e-112, Method: Compositional matrix adjust. Identities = 198/235 (84%), Positives = 218/235 (92%) Query: 133 RVRSXAENALALLVGTNVKDLPAGQDLSLQNIVTDIPAGVPSELLTRRPDIRSAEQKLLA 192 R R+ AENAL LLVG + +LP Q LS + IVTDIPAG+PS+LLT RPDIRSAEQ LLA Sbjct: 247 RQRAQAENALTLLVGKKIGELPPAQLLSDEKIVTDIPAGLPSDLLTNRPDIRSAEQSLLA 306 Query: 193 ANANIGAARAAFFPRISLTGSFGSASSSLGGLFDSGSGAWSYGPQLSLPIFDFGRNSANL 252 ANANIGAARAAFFPRISLTG+FGSASS+LGGLF+SGSGAWS+GP LSLPIFDFGRNSANL Sbjct: 307 ANANIGAARAAFFPRISLTGNFGSASSTLGGLFESGSGAWSFGPSLSLPIFDFGRNSANL 366 Query: 253 DVAEVRKNQAIVTYEKTIQTAFREVADALVARGTLDEQIEAQEAFLKAQQERLNLTDLRY 312 D+AEVRKN+A+VTYEK IQTAFREVADALVARG+LDEQ++AQEAFL AQQERL LTDLR+ Sbjct: 367 DLAEVRKNKAVVTYEKAIQTAFREVADALVARGSLDEQVKAQEAFLAAQQERLKLTDLRF 426 Query: 313 KNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYRALGGGLNENSVTVAA 367 KNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLY+ALGGGLNEN+V+VAA Sbjct: 427 KNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYKALGGGLNENTVSVAA 481 Score = 225 bits (573), Expect = 5e-60, Method: Compositional matrix adjust. Identities = 110/150 (73%), Positives = 124/150 (82%), Gaps = 2/150 (1%) Query: 1 MLVVGVLSGCTLAPTYERPQAPVEGSYPADAKG--QYDAATLGWRQFFPDQRLQALIAAA 58 +++ GVL GC+LAPTYERPQAPVE SYPADA G + A LGWRQFFPDQRLQ LIAAA Sbjct: 19 LVMAGVLGGCSLAPTYERPQAPVESSYPADAAGRAELQAVNLGWRQFFPDQRLQQLIAAA 78 Query: 59 LENNRDLRTAALNIEEARAQYRIARADLLPTLNATGSESRTRLSASQSTTGSPLITKSYQ 118 LENNRDLRTA LNIEEARAQY+IARADLLP LN +G+ +RTR +ASQS TG P I+ SYQ Sbjct: 79 LENNRDLRTAVLNIEEARAQYQIARADLLPNLNLSGNAARTRTAASQSLTGQPTISNSYQ 138 Query: 119 VGLAVPAYELDFFGRVRSXAENALALLVGT 148 VGLAVPAYELDFFGRVR+ + ALA + T Sbjct: 139 VGLAVPAYELDFFGRVRNLKDAALASYLST 168 >gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 491 Score = 230 bits (587), Expect = 1e-61, Method: Compositional matrix adjust. Identities = 122/218 (55%), Positives = 159/218 (72%), Gaps = 2/218 (0%) Query: 140 NALALLVGTNVKDLPAGQDLS-LQNIVTDIPAGVPSELLTRRPDIRSAEQKLLAANANIG 198 NAL LLVG+ V LP GQ + L + + G+P+ LLTRRPD+ +AE +L+AANANIG Sbjct: 258 NALTLLVGSPVV-LPVGQARAGLDAVFAPLQPGLPALLLTRRPDVAAAEHRLMAANANIG 316 Query: 199 AARAAFFPRISLTGSFGSASSSLGGLFDSGSGAWSYGPQLSLPIFDFGRNSANLDVAEVR 258 AARAAFFP ISLTG+ G+AS+ L GLF GS AW++ P ++LPIF GR NL ++EVR Sbjct: 317 AARAAFFPSISLTGNVGTASAELSGLFRDGSHAWTFSPSITLPIFTAGRLRNNLALSEVR 376 Query: 259 KNQAIVTYEKTIQTAFREVADALVARGTLDEQIEAQEAFLKAQQERLNLTDLRYKNGIAS 318 ++ A+ YEKT+QTAFREVADAL AR L +Q+E ++ ++ Q+ER L+ LRY NG A Sbjct: 377 RDLAVAAYEKTVQTAFREVADALAARQWLTQQLEIAQSAVRVQRERARLSALRYDNGAAP 436 Query: 319 SLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYRALGG 356 LD LDAQRDL SAQQ L+Q R+ L++++ LY ALGG Sbjct: 437 FLDVLDAQRDLLSAQQQLVQIRRALLSSSVALYAALGG 474 Score = 116 bits (290), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 66/144 (45%), Positives = 89/144 (61%), Gaps = 9/144 (6%) Query: 8 SGC-TLAPTYERPQAPVEGSYP------ADAKGQYDAAT--LGWRQFFPDQRLQALIAAA 58 + C +LAP Y+RP APV YP A + +AA+ L WR +F D LQALI A Sbjct: 23 TACQSLAPHYQRPAAPVAAVYPDGPVNEAAHQVANEAASPPLPWRDYFTDPPLQALITQA 82 Query: 59 LENNRDLRTAALNIEEARAQYRIARADLLPTLNATGSESRTRLSASQSTTGSPLITKSYQ 118 LEN+RDLRTAAL ++EA+A Y I ADL PT+ A R+R A + T PL+ +YQ Sbjct: 83 LENSRDLRTAALRVQEAQALYGIGHADLFPTIGAQAGLDRSRTPADLNLTRRPLVGSAYQ 142 Query: 119 VGLAVPAYELDFFGRVRSXAENAL 142 GL + ++E+D +GR+RS + AL Sbjct: 143 AGLGLSSWEIDLWGRLRSLDDAAL 166 >gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 498 Score = 222 bits (566), Expect = 4e-59, Method: Compositional matrix adjust. Identities = 123/243 (50%), Positives = 160/243 (65%), Gaps = 11/243 (4%) Query: 140 NALALLVGTNVKDLPAG--------QDLSLQNIVTDIPAGVPSELLTRRPDIRSAEQKLL 191 NAL LLVG KDLP L+ + + IP G+PS+LL RR DI AE+ L Sbjct: 258 NALVLLVG---KDLPTALLPQGLPDTRLAAASPLATIPPGLPSDLLQRRADIVQAERDLR 314 Query: 192 AANANIGAARAAFFPRISLTGSFGSASSSLGGLFDSGSGAWSYGPQLSLPIFDFGRNSAN 251 AANANIGAARAAF+P I+LT + G+AS+ L GLF GSG+WS+ PQ+SLPIFD G N AN Sbjct: 315 AANANIGAARAAFYPSITLTVNAGTASARLAGLFKGGSGSWSFVPQISLPIFDGGANQAN 374 Query: 252 LDVAEVRKNQAIVTYEKTIQTAFREVADALVARGTLDEQIEAQEAFLKAQQERLNLTDLR 311 LD+A V ++ ++ YEK IQTAFREV+DAL R TL Q++AQEA ++A E L+ R Sbjct: 375 LDIATVSRDISVAQYEKAIQTAFREVSDALAQRNTLGRQLQAQEALVQASDEAFRLSQAR 434 Query: 312 YKNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYRALGGGLNENSVTVAATPXT 371 Y +G+ S L LD+QR L+SAQ+ LI + R N + Y+ LGGG E + T AA+ Sbjct: 435 YHSGVDSYLTVLDSQRTLYSAQKNLIGTQLSRWTNLVTFYKTLGGGWVERTGTPAASDIA 494 Query: 372 TRQ 374 T++ Sbjct: 495 TQE 497 Score = 139 bits (349), Expect = 5e-34, Method: Compositional matrix adjust. Identities = 76/155 (49%), Positives = 94/155 (60%), Gaps = 7/155 (4%) Query: 1 MLVVGVLSGCTLAPTYERPQAPVEGSYP-ADAKGQYDA------ATLGWRQFFPDQRLQA 53 +++ +L GCTL P Y RP APV + AD+K A A +GWR F D LQ Sbjct: 18 VMLAMLLGGCTLMPDYHRPDAPVAAHFAGADSKNAAGASSAPVVADIGWRDVFTDPSLQQ 77 Query: 54 LIAAALENNRDLRTAALNIEEARAQYRIARADLLPTLNATGSESRTRLSASQSTTGSPLI 113 +IA ALENNRDLR A LNIE+ARAQY + RA L P++ A SE+ +R S+TGS L Sbjct: 78 VIALALENNRDLRVAVLNIEKARAQYGVQRAALFPSIKAATSETASRTPGDLSSTGSALT 137 Query: 114 TKSYQVGLAVPAYELDFFGRVRSXAENALALLVGT 148 ++SY L AYELD FGRVRS E AL + T Sbjct: 138 SRSYTATLGFSAYELDLFGRVRSLKEQALQSFLST 172 >gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 497 Score = 216 bits (551), Expect = 2e-57, Method: Compositional matrix adjust. Identities = 114/225 (50%), Positives = 156/225 (69%), Gaps = 3/225 (1%) Query: 140 NALALLVGTNVKD--LPAGQDLSLQNIVTDIPAGVPSELLTRRPDIRSAEQKLLAANANI 197 NAL LLVG ++ LPAG +++ PAG+PS++L RRPD+ +AE L +ANA+I Sbjct: 265 NALRLLVGADIDAALLPAGLP-DTASVLPATPAGLPSQVLLRRPDVLAAEHTLKSANADI 323 Query: 198 GAARAAFFPRISLTGSFGSASSSLGGLFDSGSGAWSYGPQLSLPIFDFGRNSANLDVAEV 257 GAARAAFFP ISLT + G+AS +LGGLF +GSG+W++ P ++LPIF+ G A+LD A++ Sbjct: 324 GAARAAFFPSISLTANGGTASRTLGGLFKAGSGSWTFSPSINLPIFNAGSLRASLDSAKI 383 Query: 258 RKNQAIVTYEKTIQTAFREVADALVARGTLDEQIEAQEAFLKAQQERLNLTDLRYKNGIA 317 ++ + YEK IQTAFREVADAL R TLD++++AQ A ++ L+D RY+NG+ Sbjct: 384 TRDIDVANYEKAIQTAFREVADALAVRATLDQRLDAQGKATAASEKAFRLSDARYRNGVD 443 Query: 318 SSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYRALGGGLNENS 362 S L L AQR L+SAQQ LI R +N I LY+ LGGG +E + Sbjct: 444 SYLTTLVAQRTLYSAQQTLISLRLTEQSNRITLYKVLGGGWSEQT 488 Score = 139 bits (349), Expect = 5e-34, Method: Compositional matrix adjust. Identities = 77/147 (52%), Positives = 98/147 (66%), Gaps = 13/147 (8%) Query: 9 GC-TLAPTYERPQAPVEGSY-------PA-----DAKGQYDAATLGWRQFFPDQRLQALI 55 GC LAP Y +P APV S+ PA A G+ DA LGWR+FF D RLQ +I Sbjct: 27 GCANLAPDYTQPAAPVPASWTSQAAAQPAVGSAGPAAGEIDADHLGWREFFLDPRLQQVI 86 Query: 56 AAALENNRDLRTAALNIEEARAQYRIARADLLPTLNATGSESRTRLSASQSTTGSPLITK 115 AAAL+NNRDLR AALNIE+ARAQYRI A+ P+++ATG+ S +R A STTGS ++ Sbjct: 87 AAALDNNRDLRVAALNIEKARAQYRITDANRYPSISATGTSSASRTPAELSTTGSAAVSH 146 Query: 116 SYQVGLAVPAYELDFFGRVRSXAENAL 142 V + V AYELDFFGR+++ ++ AL Sbjct: 147 QQSVTVGVSAYELDFFGRLKNLSDAAL 173 >gi|300310854|ref|YP_003774946.1| NodT family protein [Herbaspirillum seropedicae SmR1] Length = 507 Score = 141 bits (356), Expect = 8e-35, Method: Compositional matrix adjust. Identities = 88/235 (37%), Positives = 134/235 (57%), Gaps = 7/235 (2%) Query: 132 GRVRSXAENALALLVGTNVKDLPAGQDLS---LQNIVTDIPAGVPSELLTRRPDIRSAEQ 188 G R+ E+A+ALLVG PA L L + +P GVPS LL RRPD+ +AE+ Sbjct: 253 GVTRAQVEHAIALLVGKT----PAQFALPRAPLAAVAPPVPQGVPSMLLERRPDVAAAER 308 Query: 189 KLLAANANIGAARAAFFPRISLTGSFGSASSSLGGLFDSGSGAWSYGPQLSLPIFDFGRN 248 ++ +ANA IG A+AA+FP ++L+ S G SS+ F WS GPQL+ IFD G Sbjct: 309 RVASANAKIGVAKAAYFPSLTLSASGGYQSSTFADWFTLPGRVWSLGPQLAQTIFDGGAR 368 Query: 249 SANLDVAEVRKNQAIVTYEKTIQTAFREVADALVARGTLDEQIEAQEAFLKAQQERLNLT 308 +A D A + + TY++T+ TAF+EV D L A L+++ Q L+A ++ + L Sbjct: 369 AAATDQAIATYDGTVATYKQTVLTAFQEVEDNLAALRILEQEAAVQGEALQAARQAVTLV 428 Query: 309 DLRYKNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYRALGGGLNENSV 363 + +YK G S ++ + AQ SA+ + + R+ ++ L +ALGGG +E S+ Sbjct: 429 NNQYKAGTVSYVNVITAQATALSAELSSLNILNRRMAASVLLAKALGGGWDERSL 483 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.131 0.362 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 978,812 Number of extensions: 36065 Number of successful extensions: 207 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 180 Number of HSP's successfully gapped: 28 Length of query: 393 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 306 Effective length of database: 1,207,925 Effective search space: 369625050 Effective search space used: 369625050 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)