BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0151.0007.p
         (303 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300311906|ref|YP_003775998.1| L-lactate dehydrogenase [Herbas...   391   e-110
gi|300309586|ref|YP_003773678.1| FMN-dependent L-lactate dehydro...   122   3e-29

>gi|300311906|ref|YP_003775998.1| L-lactate dehydrogenase
           [Herbaspirillum seropedicae SmR1]
          Length = 380

 Score =  391 bits (1005), Expect = e-110,   Method: Compositional matrix adjust.
 Identities = 185/197 (93%), Positives = 194/197 (98%)

Query: 1   MPVITCVEDLRVLAKKRVPKAFYDYADSGSYTESTYRANTDDLAALKLRQRVAINVDQRS 60
           MPV+TCVED R+LAKKRVPKAFYDYADSGSYTESTYRAN+ DLAALKLRQRVAINVD+RS
Sbjct: 1   MPVMTCVEDFRLLAKKRVPKAFYDYADSGSYTESTYRANSKDLAALKLRQRVAINVDERS 60

Query: 61  TRTTMIGHDVAMPVAIAPTGLTGMQWANGEMLGAMAAEKFGIPFTLSTMSICSIEDVASV 120
           TRTTMIGHDV MPVAIAPTGLTGMQWANGEMLGA+AAEKFGIPFTLSTMSICSIEDVASV
Sbjct: 61  TRTTMIGHDVTMPVAIAPTGLTGMQWANGEMLGAIAAEKFGIPFTLSTMSICSIEDVASV 120

Query: 121 TTKPFWFQLYVMRDRGFVKSMIDRAKAAKCSALVLTLDLQILGQRHKDLKNGMSVPPKLT 180
           TTKPFWFQLYVMRDRGFVKS+I+RAKAAKCSALVLTLDLQILGQRHKDLKNGMSVPPKLT
Sbjct: 121 TTKPFWFQLYVMRDRGFVKSLIERAKAAKCSALVLTLDLQILGQRHKDLKNGMSVPPKLT 180

Query: 181 LDTLMDLASKPGWALRA 197
           L+TL+DLASKPGWALRA
Sbjct: 181 LETLLDLASKPGWALRA 197



 Score =  182 bits (463), Expect = 2e-47,   Method: Compositional matrix adjust.
 Identities = 88/99 (88%), Positives = 97/99 (97%)

Query: 203 RQLDGAMSSIEALPAIAEAVGDQIEVWFDGGIRTGQDILKATALGAKGTMIGRAFLYSLG 262
           RQLDGAMSSIEALPAIA+AVGDQIEVWFDGGIR+GQD+LKA ALGA+GTMIGRAFLYSLG
Sbjct: 281 RQLDGAMSSIEALPAIAQAVGDQIEVWFDGGIRSGQDVLKAVALGARGTMIGRAFLYSLG 340

Query: 263 AMGGEGVTKMLQILHKELDVSMALTGTKDIRDVGPQILI 301
           AMGGEGV++ML+I+ KELDVSMALTGTKDI+DVGPQILI
Sbjct: 341 AMGGEGVSQMLEIMRKELDVSMALTGTKDIKDVGPQILI 379


>gi|300309586|ref|YP_003773678.1| FMN-dependent L-lactate
           dehydrogenase [Herbaspirillum seropedicae SmR1]
          Length = 413

 Score =  122 bits (306), Expect = 3e-29,   Method: Compositional matrix adjust.
 Identities = 65/187 (34%), Positives = 100/187 (53%), Gaps = 1/187 (0%)

Query: 7   VEDLRVLAKKRVPKAFYDYADSGSYTESTYRANTDDLAALKLRQRVAINVDQRSTRTTMI 66
           +E+LR +A+KRVP   ++Y + G+  E + R N +    +    R  ++V  R     + 
Sbjct: 37  IEELRAMARKRVPNFCFEYVEGGAEEEISLRHNREVFTRIGFLPRTLVDVSVRRQGRRLF 96

Query: 67  GHDVAMPVAIAPTGLTGMQWANGEMLGAMAAEKFGIPFTLSTMSICSIEDVASVTTKPFW 126
           G D+A P  I PTG +G+    G++  A AA   G+PF L+ +S  S+E+V   +    W
Sbjct: 97  GQDIASPFLIGPTGFSGLLAREGDVAMASAAASAGVPFVLTNVSTTSLEEVVRRSGAQVW 156

Query: 127 FQLYVMRDRGFVKSMIDRAKAAKCSALVLTLDLQILGQRHKDLKNGMSVPPKLTLDTLMD 186
            Q+Y+ RDR FV S+  RA+AA    LVLT D  + G+R  D +N  S P +L     +D
Sbjct: 157 QQVYLYRDRAFVASVAQRAQAAGIGVLVLTTDSAVYGKREWDARN-FSSPRRLDWRNKLD 215

Query: 187 LASKPGW 193
           +   P W
Sbjct: 216 VLRHPRW 222



 Score = 75.5 bits (184), Expect = 4e-15,   Method: Compositional matrix adjust.
 Identities = 44/90 (48%), Positives = 57/90 (63%)

Query: 203 RQLDGAMSSIEALPAIAEAVGDQIEVWFDGGIRTGQDILKATALGAKGTMIGRAFLYSLG 262
           RQLDGA+S+++ LP +  AV  Q+ V  DGG R G DI+KA ALGA   ++GRA  Y L 
Sbjct: 311 RQLDGALSTMDVLPEVVAAVKGQLTVMLDGGFRRGADIVKAIALGADAVLLGRATTYGLA 370

Query: 263 AMGGEGVTKMLQILHKELDVSMALTGTKDI 292
           A G  G T+ L+IL  E+D  +AL    DI
Sbjct: 371 AGGQAGATRALEILRSEVDRVLALLACPDI 400


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.321    0.135    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 868,764
Number of extensions: 33565
Number of successful extensions: 114
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 114
Number of HSP's successfully gapped: 4
Length of query: 303
Length of database: 1,619,870
Length adjustment: 85
Effective length of query: 218
Effective length of database: 1,217,395
Effective search space: 265392110
Effective search space used: 265392110
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 130 (54.7 bits)