BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0150.0034.p (406 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309596|ref|YP_003773688.1| acyl-CoA dehydrogenase [Herbasp... 765 0.0 gi|300313303|ref|YP_003777395.1| acyl-CoA dehydrogenase [Herbasp... 426 e-120 gi|300310835|ref|YP_003774927.1| acyl-CoA dehydrogenase [Herbasp... 372 e-104 gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 100 3e-22 gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [H... 73 4e-14 >gi|300309596|ref|YP_003773688.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 596 Score = 765 bits (1976), Expect = 0.0, Method: Compositional matrix adjust. Identities = 355/404 (87%), Positives = 385/404 (95%) Query: 1 MPQYNAPLRDMQFVLHELLKVEDVLKTLPAHADTNRELIDQVLEEGGKFTSQVLFPLNHS 60 MPQYNAPLRDMQFVLHELL VE+V K LP HADTNREL+DQVLEEGGKF SQVLFPLNHS Sbjct: 1 MPQYNAPLRDMQFVLHELLNVEEVFKELPPHADTNRELVDQVLEEGGKFASQVLFPLNHS 60 Query: 61 GDREGCTYNKDTKTVTPPKGYKEAYKQYVEAGWAALSCDPEFGGQGLPLVMNNSFYEMLN 120 GDREGC Y++++KTVT PKG+K+AY+QYVEAGWAALSCDPEFGGQGLPL +NNSFYEMLN Sbjct: 61 GDREGCHYDRESKTVTTPKGFKQAYQQYVEAGWAALSCDPEFGGQGLPLTINNSFYEMLN 120 Query: 121 SSNQAWTMYPGLSHGAYECIHAHGSDEQKALYLPKLISGEWTGTMCLTEAHCGTDLGLLR 180 SSNQAWTMYPGLSHGAYEC+HAHGS+EQK LYLPKL+SGEWTGTMCLTEAHCGTDLGLLR Sbjct: 121 SSNQAWTMYPGLSHGAYECLHAHGSEEQKQLYLPKLVSGEWTGTMCLTEAHCGTDLGLLR 180 Query: 181 SKAEPQADGSYKITGSKIFISAGEHDMSDNIVHLVLARLPDAPQGSKGISLFIVPKFMPN 240 SKAEPQADGSYKITG+KIFISAGE+D+S NI+HLVLARLPDAP+GSKGISLF+VPKF+PN Sbjct: 181 SKAEPQADGSYKITGNKIFISAGENDVSANIIHLVLARLPDAPEGSKGISLFLVPKFLPN 240 Query: 241 ADGSLGSRNPITCGAIEEKMGIHGNATCQMNLDGAVGWLIGGPHKGLNAMFVFMNAARLG 300 ADGSLG+RNPI CGAIEEKMGIHGNATCQMNLDGA GWLIG PHKGL AMFVFMNAARLG Sbjct: 241 ADGSLGARNPIFCGAIEEKMGIHGNATCQMNLDGATGWLIGQPHKGLQAMFVFMNAARLG 300 Query: 301 VGMQGLGLTEVAYQNALVYAKDRIQMRSLSGVKAPDKAADPIIVHPDVRRMLLTAKAYAE 360 VGMQGLGLTEVAYQNALVYAKDR+QMRSLSG+KA DK ADPIIVHPDVRRMLLTAKA+AE Sbjct: 301 VGMQGLGLTEVAYQNALVYAKDRLQMRSLSGIKAADKPADPIIVHPDVRRMLLTAKAFAE 360 Query: 361 GGRAFCSYVALQLDKELNHPDEEVRKEAADQVALLTPVVKAFIS 404 GGRAF SYVAL+LDKEL HPDE+VRK++AD VALLTPVVKAF++ Sbjct: 361 GGRAFTSYVALELDKELTHPDEQVRKDSADIVALLTPVVKAFLT 404 >gi|300313303|ref|YP_003777395.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 591 Score = 426 bits (1096), Expect = e-120, Method: Compositional matrix adjust. Identities = 209/403 (51%), Positives = 274/403 (67%), Gaps = 14/403 (3%) Query: 3 QYNAPLRDMQFVLHELLKVEDVLKTLPAHADTNRELIDQVLEEGGKFTSQVLFPLNHSGD 62 Y PLRD FVL E+L+ VL+ LPAHA+ + +L+ QVLEE G+F + V+ PLN GD Sbjct: 4 HYAPPLRDFAFVLDEVLQAPQVLRALPAHAEIDGDLMMQVLEEAGRFAADVVAPLNGIGD 63 Query: 63 REGCTYNKDTKTVTPPKGYKEAYKQYVEAGWAALSCDPEFGGQGLPLVMNNSFYEMLNSS 122 REGC + TVT P G++ AY Q+ GW AL+C PE GGQGLP V++ + YEML+++ Sbjct: 64 REGCRFK--NGTVTTPPGFERAYAQFCAGGWPALACAPEHGGQGLPHVLDCALYEMLSAA 121 Query: 123 NQAWTMYPGLSHGAYECIHAHGSDEQKALYLPKLISGEWTGTMCLTEAHCGTDLGLLRSK 182 N WTMYPGL HGAY C+ AHGS +A YL K+ SGEW TMCLTEA G+DLG LR++ Sbjct: 122 NHGWTMYPGLLHGAYACLAAHGSAALQARYLDKIASGEWLATMCLTEAQAGSDLGQLRTR 181 Query: 183 AEPQADGSYKITGSKIFISAGEHDMSDNIVHLVLARLPDAPQGSKGISLFIVPKFMPNAD 242 A PQ DGSY+++G KIFIS GEHD++ NIVHLVLARLPDAP GSKG+SLF+ P+ + + Sbjct: 182 AVPQDDGSYRLSGDKIFISGGEHDLTPNIVHLVLARLPDAPAGSKGLSLFLAPRVLDD-- 239 Query: 243 GSLGSRNPITCGAIEEKMGIHGNATCQMNLDGAVGWLIGGPHKGLNAMFVFMNAARLGVG 302 GSRN + C IE KMGIHG+ATC ++ + A WL+G +GL AMFV MNAARL VG Sbjct: 240 ---GSRNGVHCTGIEHKMGIHGSATCSLHFEQAQAWLVGEAGRGLAAMFVMMNAARLHVG 296 Query: 303 MQGLGLTEVAYQNALVYAKDRIQMRSLSGVKAPDKAADPIIV-HPDVRRMLLTAKAYAEG 361 QGLGL E AYQ A YA++R Q R AP + + +IV HP V+++L+ + EG Sbjct: 297 AQGLGLAEAAYQRASAYARERRQSR------APGSSGNDLIVHHPAVQKLLMEQRCRIEG 350 Query: 362 GRAFCSYVALQLDKELNHPDEEVRKEAADQVALLTPVVKAFIS 404 R + L LD + P+ +R +++ +TPV+KAF++ Sbjct: 351 ARLLTCWAGLLLDLAEHDPEAALRSRHHERLEFITPVIKAFLT 393 >gi|300310835|ref|YP_003774927.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 594 Score = 372 bits (956), Expect = e-104, Method: Compositional matrix adjust. Identities = 192/404 (47%), Positives = 259/404 (64%), Gaps = 10/404 (2%) Query: 4 YNAPLRDMQFVLHELLKVEDVLKTLPAHADTNRELIDQVLEEGGKFTSQVLFPLNHSGDR 63 Y AP++DM FV++EL + V LP D E + +LEE KF S+V+ PLNHS D+ Sbjct: 3 YTAPVKDMLFVMNELAGLAAV-NALPGCEDATPETAEAILEENAKFCSEVVAPLNHSADK 61 Query: 64 EGCTYNKDTKTVTPPKGYKEAYKQYVEAGWAALSCDPEFGGQGLPLVMNNSFYEMLNSSN 123 E ++ D K T P G+KEA++Q+ EAGW + EFGGQGLP ++ EMLNS+N Sbjct: 62 EPSSW-ADGKVTTSP-GFKEAFRQFGEAGWQGVQHPVEFGGQGLPKLLATPCIEMLNSAN 119 Query: 124 QAWTMYPGLSHGAYECIHAHGSDEQKALYLPKLISGEWTGTMCLTEAHCGTDLGLLRSKA 183 ++ + P L+ G E + G+ +QK ++ ISG+WTGTM LTE G+DL L+R++A Sbjct: 120 LSFALCPLLTDGTIEALMTAGTQQQKETFIANFISGKWTGTMNLTEPQAGSDLALVRTRA 179 Query: 184 EPQADGSYKITGSKIFISAGEHDMSDNIVHLVLARLPDAPQGSKGISLFIVPKFMPNADG 243 P+ DG+YK+ G+KIFI+ GEHDM++NIVHLVLAR P+AP+G KGISLFIVPKF+ NADG Sbjct: 180 VPEGDGTYKLFGTKIFITYGEHDMAENIVHLVLARTPNAPEGVKGISLFIVPKFLVNADG 239 Query: 244 SLGSRNPITCGAIEEKMGIHGNATCQMNL---DGAVGWLIGGPHKGLNAMFVFMNAARLG 300 SLG+RN + C +IE K+GI + T + GA+G L+G ++GL MF+ MNAAR Sbjct: 240 SLGARNDVHCVSIEHKLGIKASPTAVLQFGDHGGAIGTLVGEENRGLEYMFIMMNAARFA 299 Query: 301 VGMQGLGLTEVAYQNALVYAKDRIQMRSLSGVKAPDKAADPIIVHPDVRRMLLTAKAYAE 360 VG+QGL ++E AYQ A+ YA+DRIQ R L+G P II PDVRRML++ +A E Sbjct: 300 VGIQGLSVSERAYQKAVAYARDRIQSRDLAGSPGPVA----IIHQPDVRRMLMSMRAQIE 355 Query: 361 GGRAFCSYVALQLDKELNHPDEEVRKEAADQVALLTPVVKAFIS 404 RA A D DE RK L P+VK +I+ Sbjct: 356 AARALSYVAAATSDAAHFSDDEATRKSNQAFYEYLVPIVKGWIT 399 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 100 bits (248), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 74/245 (30%), Positives = 113/245 (46%), Gaps = 20/245 (8%) Query: 83 EAYKQYVEAGWAALSCDPEFGGQGLP-LVMNNSFYEMLNSSNQAWTMYPGLSHGAYECIH 141 EA K+ E G + ++GG G+ + + + E+ T+ + A Sbjct: 38 EALKELAELGAMGMCVPEQWGGAGMDYMSLVLALEEIAAGDGATSTIVSVQNSLACGITE 97 Query: 142 AHGSDEQKALYLPKLISGEWTGTMCLTEAHCGTDLGLLRSKAEPQADGSYKITGSKIFIS 201 +GS QK +L L GE G CLTE H G+D +R++AE D + + G+K FI+ Sbjct: 98 KYGSPTQKEQWLKPLARGEMLGCFCLTEPHTGSDAAAIRTRAERDGD-DFILNGTKQFIT 156 Query: 202 AGEHDMSDNIVHLVLARLPDAPQGSKGISLFIVPKFMPNADGSLGSRNPITCGAIEEKMG 261 +G+H V +V A + D G KGIS F+VP P G EEKMG Sbjct: 157 SGKHAG----VAIVFA-VTDRSAGKKGISCFLVPCDTPG----------FVVGRSEEKMG 201 Query: 262 IHGNATCQMNLDGA---VGWLIGGPHKGLNAMFVFMNAARLGVGMQGLGLTEVAYQNALV 318 H + T Q+ LD L+G +G + A R+G+ Q +G+ A++ A+ Sbjct: 202 QHASDTVQIMLDNCRVPATALLGKEGEGYRIALSNLEAGRIGIAAQSVGMARAAFEAAVS 261 Query: 319 YAKDR 323 YA+ R Sbjct: 262 YARQR 266 >gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 394 Score = 73.2 bits (178), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 63/250 (25%), Positives = 126/250 (50%), Gaps = 28/250 (11%) Query: 85 YKQYVEAGWAALSCDPEFGGQGLPLVMNNSFYEMLNSSNQAWTMYPGLSHGAYE--C--- 139 +K+ + G ++ E+GG GL + + E ++ ++ + GLS+GA+ C Sbjct: 48 WKKLGDLGVLGITVSEEYGGAGLGYLAHIIAMEEISRASAS----VGLSYGAHSNLCVNQ 103 Query: 140 IHAHGSDEQKALYLPKLISGEWTGTMCLTEAHCGTDLGLLRSKAEPQADGSYKITGSKIF 199 I +G++EQK YLP+LISG++ G + ++E + G+D+ ++ +A+ + D Y + GSK++ Sbjct: 104 IKRNGNEEQKRKYLPRLISGDFIGALAMSEPNAGSDVVSMKLRADKKGD-RYVLNGSKMW 162 Query: 200 ISAGEHDMSDNIVHLVLARLPDAPQGSKGISLFIVPKFMPNADGSLGSRNPITCGAIEEK 259 I+ G D V +V A+ D G++G++ F+V K + +K Sbjct: 163 ITNG----PDADVLVVYAK-TDLEAGARGMTAFLVEK----------GYKGFSVAQKLDK 207 Query: 260 MGIHGNATCQM---NLDGAVGWLIGGPHKGLNAMFVFMNAARLGVGMQGLGLTEVAYQNA 316 +G+ G+ T ++ + + ++GG +G+N + ++ R + LG+ + Sbjct: 208 LGMRGSHTGELVFQDCEVPEENVLGGVGRGVNVLMSGLDFERSVLSGGPLGIMQACMDVV 267 Query: 317 LVYAKDRIQM 326 + Y DR Q Sbjct: 268 VPYVHDRKQF 277 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,365,877 Number of extensions: 60257 Number of successful extensions: 155 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 140 Number of HSP's successfully gapped: 8 Length of query: 406 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 319 Effective length of database: 1,207,925 Effective search space: 385328075 Effective search space used: 385328075 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)