BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0150.0027.p (485 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309796|ref|YP_003773888.1| methyl-accepting chemotaxis pro... 320 1e-88 gi|300310789|ref|YP_003774881.1| methyl-accepting chemotaxis tra... 266 3e-72 gi|300313925|ref|YP_003778017.1| methyl-accepting chemotaxis sen... 264 1e-71 gi|300312783|ref|YP_003776875.1| methyl-accepting chemotaxis tra... 262 4e-71 gi|300309873|ref|YP_003773965.1| methyl-accepting chemotaxis tra... 243 3e-65 >gi|300309796|ref|YP_003773888.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 563 Score = 320 bits (820), Expect = 1e-88, Method: Compositional matrix adjust. Identities = 173/381 (45%), Positives = 257/381 (67%), Gaps = 7/381 (1%) Query: 1 LKISTRLAIGFTMVCALLIIITLLGIIQMAQQKQRMDEITKINNVEASEASSMYLSITER 60 +K+ TRL +GF V LL+++T GI M Q + R + ++ N E++ M ++ ER Sbjct: 5 MKMGTRLGLGFAWVVLLLVLVTGFGIFNMHQVQGRFTHVVEVKNPESALVKEMLETVGER 64 Query: 61 ALALRNMILLEDNQQEISVEMKRVDVQAQKYAAAEKKLGEMLSSEGSANPEERQLFDAIS 120 +++LRN+ +L +++ +E + ++ Q+ KY A +KL ++ + + EE+ I+ Sbjct: 65 SISLRNL-MLPAAPEDVDIEAQYIEAQSLKYKMAAQKLQQLFAESSDTSDEEKAALPKIA 123 Query: 121 ATAKVADGYIKRVTTLILAGKRDEAYSL---MRNEFRPVQRKWWEQLNSFIVLENKQNEQ 177 A A A+ I GKR EAY L ++ ++ PVQ+ W +L + LE++ N++ Sbjct: 124 AQASKAEKLINEAVEF---GKRREAYELAQFLKEQYLPVQKSWQVELKALAALEDQLNKE 180 Query: 178 ASDNADLAYANARFMMLLFGGLAVVASIIAAYLITRSVLSQLGGEPAYANQIANQIAHGD 237 A++ + +AY+ AR +MLL + VV +++AA+ +TR +L +LGGEP YA QIA QIA GD Sbjct: 181 AAEASAVAYSRARMLMLLISAIGVVTAVLAAFWLTRHLLRKLGGEPDYAVQIAGQIAEGD 240 Query: 238 LTVNIETRPSDQNSLLFSIKSMRDALAQIVGQVRAGTDTIATASSQIAKGNLDLSSRTEQ 297 L V I+TR D +SL+ +++ MRD LA IV +VR G++TI TASS+IA+GNLDLS RTEQ Sbjct: 241 LAVVIDTRAGDHDSLMAAMRVMRDKLAAIVAEVRKGSETITTASSEIARGNLDLSGRTEQ 300 Query: 298 QASSLEETASSMEELASTVKQNSGSALQAHELALSASDVATKAGEVVQQVINTMGMINNS 357 QA +LEETASSME+L STVKQN+ +A QA++LAL+ASDVA + G +V QV+ TM IN S Sbjct: 301 QAGALEETASSMEQLNSTVKQNAENAHQANDLALAASDVAVQGGSIVSQVVQTMQSINAS 360 Query: 358 SHKIVEIISVIDGIAFQTNIL 378 S K+ +I+SVIDGIAFQTNIL Sbjct: 361 SQKVADIVSVIDGIAFQTNIL 381 >gi|300310789|ref|YP_003774881.1| methyl-accepting chemotaxis transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 542 Score = 266 bits (680), Expect = 3e-72, Method: Compositional matrix adjust. Identities = 171/378 (45%), Positives = 243/378 (64%), Gaps = 3/378 (0%) Query: 1 LKISTRLAIGFTMVCALLIIITLLGIIQMAQQKQRMDEITKINNVEASEASSMYLSITER 60 LK+STRLA+GF V LL++I+L+G+ MA K + +I NN + A M S+ R Sbjct: 4 LKVSTRLALGFASVVVLLVVISLVGLWGMATVKGNLSDIVA-NNQQGGLADVMNNSVRIR 62 Query: 61 ALALRNMILLEDNQQEISVEMKRVDVQAQKYAAAEKKLGEMLSSEGSANPEERQLFDAIS 120 ALA+RN+ L ++ I+ E++R+ VQ + Y A +KLG+M + E +LFD I+ Sbjct: 63 ALAIRNIALFQEPAL-IAREIERIKVQEKAYQDAREKLGKMFDQPDTTR-REHELFDLIA 120 Query: 121 ATAKVADGYIKRVTTLILAGKRDEAYSLMRNEFRPVQRKWWEQLNSFIVLENKQNEQASD 180 A + + + TL K E SL+ + RPVQ KW + L+ E++ N QAS Sbjct: 121 QNAADTEPLMNQAVTLAQQDKSAELISLLTQKIRPVQAKWLDNLDELGRFEDELNNQASA 180 Query: 181 NADLAYANARFMMLLFGGLAVVASIIAAYLITRSVLSQLGGEPAYANQIANQIAHGDLTV 240 AD Y + +V+ ++ AA+LI RSVL QLGGEPA A ++A QIA G+L V Sbjct: 181 AADTTYRSILTTTWTLVAGSVLLAVAAAFLIVRSVLHQLGGEPAQAQELAAQIAQGNLAV 240 Query: 241 NIETRPSDQNSLLFSIKSMRDALAQIVGQVRAGTDTIATASSQIAKGNLDLSSRTEQQAS 300 +I DQ+SL+ S+++MR L +IV ++ ++I+TA+SQIA GNLDLS RTE+QA+ Sbjct: 241 SIPLAKGDQHSLMASLENMRAQLTRIVDGIKHSAESISTAASQIAVGNLDLSRRTEEQAA 300 Query: 301 SLEETASSMEELASTVKQNSGSALQAHELALSASDVATKAGEVVQQVINTMGMINNSSHK 360 SLEETASSMEE+ STV+QN+ +A Q H+LA SAS A K GEVV++V+ TM I+ S++K Sbjct: 301 SLEETASSMEEITSTVEQNTQNAGQGHQLARSASLHAQKGGEVVERVVGTMKDISQSAYK 360 Query: 361 IVEIISVIDGIAFQTNIL 378 I EII+ ++GIAFQTNIL Sbjct: 361 ITEIIATVEGIAFQTNIL 378 >gi|300313925|ref|YP_003778017.1| methyl-accepting chemotaxis sensory transducer protein [Herbaspirillum seropedicae SmR1] Length = 522 Score = 264 bits (674), Expect = 1e-71, Method: Compositional matrix adjust. Identities = 165/387 (42%), Positives = 248/387 (64%), Gaps = 19/387 (4%) Query: 1 LKISTRLAIGFTMVCALLIIITLLGIIQMAQQKQRMDEITKINNVEASEASSMYLSITER 60 L +S+RL GF +V L+++I + + ++A+ +++ IT++NNVE S+M + E+ Sbjct: 6 LTVSSRLKFGFGVVLFLILLIVGIALKKLAEVNRQLVSITEVNNVEIYHLSTMRAAAYEQ 65 Query: 61 ALALRNMILL---EDNQQEISVEMKRVDVQAQKYAAAEKKLGEMLSSEGSANPEERQLFD 117 +LA R + L ED Q + +++ + Y +E L ++ + E+Q Sbjct: 66 SLASRGIGLALTPEDLNQNANNLQRQIGL----YTQSEAALAKLFADLQETTDTEKQ--- 118 Query: 118 AISATAKVADGY---IKRVTTLILAGKRDEAYSLMRNEFRPVQ---RKWWEQLNSFIVLE 171 A++A K++ G + + L A K +E +M +Q RK +L++F E Sbjct: 119 ALAAIQKMSAGLSPGLSNMIDLAKAQKTEELKPIMTGPLGKMQADRRKALAELSAF---E 175 Query: 172 NKQNEQASDNADLAYANARFMMLLFGGLAVVASIIAAYLITRSVLSQLGGEPAYANQIAN 231 +K N +A D A A+AR +++ G +A++ ++AA LITRSVL QLGGEPAYA+++A Sbjct: 176 DKLNNEAKDEALAVSASARKLLIALGVIALLCGLMAAVLITRSVLRQLGGEPAYASRLAA 235 Query: 232 QIAHGDLTVNIETRPSDQNSLLFSIKSMRDALAQIVGQVRAGTDTIATASSQIAKGNLDL 291 QIA GDL ++ SL+ S+K+M + L IV +VR+GTD IATASSQIA GNLDL Sbjct: 236 QIAKGDLRTEVKADTRHPESLMMSMKTMNEGLRGIVREVRSGTDAIATASSQIASGNLDL 295 Query: 292 SSRTEQQASSLEETASSMEELASTVKQNSGSALQAHELALSASDVATKAGEVVQQVINTM 351 SSRTE+QASSLEETAS+ME+L +TVKQN+ SA +A+ LA AS VA+++G++V++V+ TM Sbjct: 296 SSRTEEQASSLEETASAMEQLTATVKQNADSAGEANRLAAEASAVASQSGQLVKEVVTTM 355 Query: 352 GMINNSSHKIVEIISVIDGIAFQTNIL 378 I +SS KIV+IISVIDGI+FQTNIL Sbjct: 356 AAIEDSSKKIVDIISVIDGISFQTNIL 382 >gi|300312783|ref|YP_003776875.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 542 Score = 262 bits (670), Expect = 4e-71, Method: Compositional matrix adjust. Identities = 171/385 (44%), Positives = 240/385 (62%), Gaps = 23/385 (5%) Query: 1 LKISTRLAIGFTMVCALLIIITLLGIIQMAQQKQRMDEITKINNVEASEASSMYLS---I 57 +KI T+LA+GF AL+++ L+ I ++ + I + V+ + + +Y + + Sbjct: 5 MKIGTKLALGF---GALVVLTILVSGISLSSIRALTSSIKAVLAVQDQKLAPLYAAREAL 61 Query: 58 TERALALRNMILLE---DNQQEISVEMKRVDVQAQKYAAAEKKLGEMLSSEGSANPEERQ 114 + +A RN + D Q+E+++ VD Q Q Y A K LG + + N E Sbjct: 62 DQTGIAARNAYVFRSDADAQKELAI----VDEQKQLYLKAIKDLGPLFA----GNKE--- 110 Query: 115 LFDAI-SATAKVADGYIKRVTTLILAGKRDEAYSLMRNEFRPVQRKWWEQLNSFIVLENK 173 F+ + S ++A+ +KR +G+ +E + NE P++R+ + + + Sbjct: 111 -FEKVRSGMLQMAE-ELKRPRLYRESGRSEEYRDFLVNECSPLRRQIVSDIGTVLAQVEA 168 Query: 174 QNEQASDNADLAYANARFMMLLFGGLAVVASIIAAYLITRSVLSQLGGEPAYANQIANQI 233 +N +A D D A + + + VV SI+ A L+TR +L QLGGEP YA QIA QI Sbjct: 169 ENNRAQDATDKMAQLAALTVAVLTLVVVVISILIAVLLTRGLLKQLGGEPTYAAQIAQQI 228 Query: 234 AHGDLTVNIETRPSDQNSLLFSIKSMRDALAQIVGQVRAGTDTIATASSQIAKGNLDLSS 293 A GDLT N++T+ D +SLLF+IK+MRD+L IV QVR GT TI ASS+IA GNLDLSS Sbjct: 229 ADGDLTANVQTKTGDNSSLLFAIKNMRDSLVSIVAQVRQGTFTIEQASSEIASGNLDLSS 288 Query: 294 RTEQQASSLEETASSMEELASTVKQNSGSALQAHELALSASDVATKAGEVVQQVINTMGM 353 RTEQQASSLEETAS+MEEL +TVKQN+ +A QA +LA+SAS+VA++ GEVV QV+ TM Sbjct: 289 RTEQQASSLEETASAMEELMTTVKQNADNAKQASQLAVSASEVASQGGEVVAQVVTTMDG 348 Query: 354 INNSSHKIVEIISVIDGIAFQTNIL 378 IN SS KIV+IISVIDGIAFQTNIL Sbjct: 349 INASSRKIVDIISVIDGIAFQTNIL 373 >gi|300309873|ref|YP_003773965.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 547 Score = 243 bits (619), Expect = 3e-65, Method: Compositional matrix adjust. Identities = 148/378 (39%), Positives = 225/378 (59%), Gaps = 1/378 (0%) Query: 1 LKISTRLAIGFTMVCALLIIITLLGIIQMAQQKQRMDEITKINNVEASEASSMYLSITER 60 LK+ TRLA+GF ++ L+ I+ LG+ MA+ + +IT +NN E +++ ++++R Sbjct: 4 LKVVTRLALGFGLILVFLLSISALGLYGMARVNDALTDITDVNNAEIKLVTALRNALSQR 63 Query: 61 ALALRNMILLEDNQQEISVEMKRVDVQAQKYAAAEKKLGEMLSSEGSANPEERQLFDAIS 120 ALA+RN+ LL D Q ++ VE + + Q + YA A L +M ++E + ERQL I Sbjct: 64 ALAIRNIALL-DEQADMQVESQTLARQEKLYADAYAALDKMFTTEAATTERERQLLVQIK 122 Query: 121 ATAKVADGYIKRVTTLILAGKRDEAYSLMRNEFRPVQRKWWEQLNSFIVLENKQNEQASD 180 + + L L K EA ++ + RP Q+ W + L E++ N++A+ Sbjct: 123 NDEAATLPLMAKAVQLGLENKAAEAGKMLITQVRPKQQAWIKSLTELAEFEDQLNDEAAT 182 Query: 181 NADLAYANARFMMLLFGGLAVVASIIAAYLITRSVLSQLGGEPAYANQIANQIAHGDLTV 240 +A YA R + + A++ + A+ LI RS+L QLG EP+ A +A IA GDLT Sbjct: 183 SARNTYAWLRMVTVAAALAALLVGVAASALIARSILRQLGAEPSEAQAVARDIASGDLTA 242 Query: 241 NIETRPSDQNSLLFSIKSMRDALAQIVGQVRAGTDTIATASSQIAKGNLDLSSRTEQQAS 300 + R D +SL+ SI+ MR L I +++ +TI+ AS QIA+GN DLS RTE+QA+ Sbjct: 243 TVRVREGDSSSLMASIEQMRYQLNVIAHGIKSAAETISVASGQIAQGNFDLSQRTEEQAA 302 Query: 301 SLEETASSMEELASTVKQNSGSALQAHELALSASDVATKAGEVVQQVINTMGMINNSSHK 360 SLEETA+SMEEL +TV+QN+ A +A L+ A++ A + +V+ TM I SS K Sbjct: 303 SLEETAASMEELTTTVRQNTQHASEALRLSGGATETAAAGSQAFDRVVQTMDRITASSSK 362 Query: 361 IVEIISVIDGIAFQTNIL 378 + +IISVI+GIAFQTNIL Sbjct: 363 MSDIISVIEGIAFQTNIL 380 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.127 0.352 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,128,198 Number of extensions: 38221 Number of successful extensions: 532 Number of sequences better than 1.0e-08: 65 Number of HSP's gapped: 425 Number of HSP's successfully gapped: 65 Length of query: 485 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 396 Effective length of database: 1,198,455 Effective search space: 474588180 Effective search space used: 474588180 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)