BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0150.0026.p (101 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310654|ref|YP_003774746.1| isoquinoline 1-oxidoreductase s... 68 1e-13 >gi|300310654|ref|YP_003774746.1| isoquinoline 1-oxidoreductase subunit alpha oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 160 Score = 68.2 bits (165), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 30/66 (45%), Positives = 43/66 (65%) Query: 3 TSFNLNGKQVTVKAEPDTPLLWVIRDEIGLTGTKFGCGMALCGACTVQLDGAPIRSCQTP 62 +F++NG V++ D PLL V+R+++ L G KFGCG+ CGACTV +DG RSC P Sbjct: 7 ITFHVNGVPVSLDIAADAPLLNVLRNDLCLNGPKFGCGLGECGACTVLVDGVAARSCVIP 66 Query: 63 VSAAAG 68 ++ A Sbjct: 67 LAGVAA 72 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.433 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 350,642 Number of extensions: 13197 Number of successful extensions: 44 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 44 Number of HSP's successfully gapped: 1 Length of query: 101 Length of database: 1,619,870 Length adjustment: 70 Effective length of query: 31 Effective length of database: 1,288,420 Effective search space: 39941020 Effective search space used: 39941020 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 123 (52.0 bits)