BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0150.0023.p (273 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309642|ref|YP_003773734.1| aldo/keto reductase [Herbaspiri... 305 2e-84 gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbasp... 82 5e-17 gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbasp... 72 6e-14 gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Her... 72 7e-14 gi|300313828|ref|YP_003777920.1| aryl-alcohol dehydrogenase [Her... 66 3e-12 >gi|300309642|ref|YP_003773734.1| aldo/keto reductase [Herbaspirillum seropedicae SmR1] Length = 280 Score = 305 bits (782), Expect = 2e-84, Method: Compositional matrix adjust. Identities = 148/216 (68%), Positives = 172/216 (79%) Query: 58 VGKAIAGRRDDVYLVSKVLPFNASRAGTVKACEASLKRLHVERIDLYLLHWRGTHPFSAT 117 V KAIAGRRD VYLVSKVLP NAS+ GT+ ACEASLKRL +RIDLYLLHWRG HP +AT Sbjct: 65 VAKAIAGRRDQVYLVSKVLPHNASKRGTIAACEASLKRLGTDRIDLYLLHWRGAHPLAAT 124 Query: 118 IEAMETLIDQGKIGAWGVSNLDNDDMHELLALDNGANVQVNQVLYNLSRRGIEFDLQPYC 177 IE METL+ QGKIG WGVSN D DD+ +LL +G + NQVLYNLSRRGIE+DL P Sbjct: 125 IEGMETLVSQGKIGGWGVSNFDTDDIDDLLEEPHGEHYLCNQVLYNLSRRGIEYDLLPQA 184 Query: 178 AQQGVAIMAYSPIEQGRILKEAALVAVAKRHGVTPAQVALAWTLRHPGVISIPKASTVQH 237 Q +A+MAYSPIEQGRILK AL A+A+ H VTPAQ+ALAW LR PGV++IPKA+ V H Sbjct: 185 QQAHIAVMAYSPIEQGRILKHPALTALAQAHRVTPAQIALAWVLRQPGVVAIPKAADVAH 244 Query: 238 VQDNRACLDLRLDAADLAGLDKAFAPPKKRRPLEML 273 VQ NRA LD+ L AADL LD+AFAPP++++PL ML Sbjct: 245 VQANRASLDISLSAADLKILDEAFAPPRRKQPLAML 280 >gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 343 Score = 82.0 bits (201), Expect = 5e-17, Method: Compositional matrix adjust. Identities = 75/270 (27%), Positives = 114/270 (42%), Gaps = 59/270 (21%) Query: 42 FLPSARAPGTWASRPXVGKAIAGRRDDVYLVSKV-----------LPFNASRAGTVKACE 90 L +A G + + +G+AI GRRD ++ +K + S A +A E Sbjct: 63 LLDTADMYGPYTNEELLGRAIKGRRDKFFIATKFGIRRDPSDPGARGVDGSPAYIRQAVE 122 Query: 91 ASLKRLHVERIDLYLLH-WRGTHPFSATIEAMETLIDQGKIGAWGVSNLDNDDM------ 143 SLKRL VE IDLY H P T+ + L+ GKI G+S + Sbjct: 123 GSLKRLGVETIDLYYQHRIDPATPIEVTMGVLADLVQAGKIRYIGLSEASAATLERAHRV 182 Query: 144 HELLALDNGANVQVNQVLYNLSRRGIEFDLQPYCAQQGVAIMAYSPIEQG---------- 193 H + AL Q Y+L R E ++ C G+ +AYSP+ +G Sbjct: 183 HPVTAL---------QSEYSLWTRDPEAEVLAACRTLGIGFVAYSPLGRGFLTGAIQRFE 233 Query: 194 --------------------RILKEAALV-AVAKRHGVTPAQVALAWTL-RHPGVISIPK 231 R L+ A V +A +HG TP+Q+ALAW + + P ++ IP Sbjct: 234 DLAEDDFRRSNPRFQGENFARNLQLAEKVKEMAGQHGCTPSQLALAWVMAQDPHIVPIPG 293 Query: 232 ASTVQHVQDNRACLDLRLDAADLAGLDKAF 261 ++++DN + ++L DL LD F Sbjct: 294 TKRRRYLEDNAGSVGVKLAPEDLQALDAIF 323 >gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 349 Score = 71.6 bits (174), Expect = 6e-14, Method: Compositional matrix adjust. Identities = 70/281 (24%), Positives = 113/281 (40%), Gaps = 74/281 (26%) Query: 45 SARAPGTWASRPXVGKAIAGRRDDVYLVSKVLP---------------FNASRAGTVKAC 89 + R GTW + +G+RD + + +K N R G +A Sbjct: 65 TERYLGTWLKK-------SGKRDQLLIATKCTGPARKPHNPRHVRGGINNLDRKGLTEAL 117 Query: 90 EASLKRLHVERIDLYLLHW------------------RGTHPFSATIEAMETLIDQGKIG 131 SL+RL ++ +DLY LHW T P T+ + + GKI Sbjct: 118 HGSLERLQLDHVDLYQLHWPDRSVNSFGQLGYQHVEDESTVPIEETLTVLSEFVKAGKIR 177 Query: 132 AWGVSNLDNDDMHELL--ALDNGANVQVN-QVLYNLSRRGIEFDLQPYCAQQGVAIMAYS 188 G+SN + + L A +G V Q YNL R E +L + ++ V ++AYS Sbjct: 178 TIGLSNETAWGVAQFLRAAEQHGLERIVTIQNPYNLLNRSFEINLAEFAHREQVGLLAYS 237 Query: 189 PIEQGRILKE------------------------------AALVAVAKRHGVTPAQVALA 218 P+ G + + A VA+A++HG+ PAQ+ALA Sbjct: 238 PLAFGMLSGKYLDGARPAGGRLTLFERFSRYTNAQAERATAEYVALARQHGLDPAQMALA 297 Query: 219 WTLRHPGVIS-IPKASTVQHVQDNRACLDLRLDAADLAGLD 258 + P + S I A+T+ ++ N +L+L LA ++ Sbjct: 298 YVNSRPFLTSNIIGATTLAQLRSNIDSAELQLGEDVLAAIE 338 >gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 335 Score = 71.6 bits (174), Expect = 7e-14, Method: Compositional matrix adjust. Identities = 72/269 (26%), Positives = 113/269 (42%), Gaps = 48/269 (17%) Query: 42 FLPSARAPGTWASRPXVGKAIAGRRDDVYLVSKV--------LPFNASRAGTVKACEASL 93 F+ +A S VGK +AG+R + L SK+ N SR+ V+ E L Sbjct: 46 FIDTADVYTKGKSEEIVGKLLAGQRHEWVLASKLGNAMSGKPNESNYSRSWIVRETENIL 105 Query: 94 KRLHVERIDLYLLHW-RGTHPFSATIEAMETLIDQGKIGAWGVSNLDNDDMHELLALDNG 152 RL + +D+ +H ++A+ LI GKI +GVSN + E++ L Sbjct: 106 TRLDTDYLDILYMHRDYQDENLEEAVQALGDLIRVGKIRGFGVSNFRGWRIAEIVRLCEK 165 Query: 153 ANV---QVNQVLYNLSRRGIEFDLQPYCAQQGVAIMAYSPIEQGRI-------------- 195 NV V Q YNL R E ++ P CA G+ ++ YSPI +G + Sbjct: 166 NNVPLPLVCQPYYNLLNRQPEVEILPACAHYGLGVVPYSPIARGVLTGKYQPGQKPAQDT 225 Query: 196 -------------LKEAALVAVAKR-------HGVTPAQVALAWTLRHPGVIS-IPKAST 234 +E +L+ +A+R GV Q A AW L + + S I T Sbjct: 226 RAGRGDKRMMETEFREESLL-IAQRLQQHCEARGVKLGQFATAWVLANSAISSVIAGPRT 284 Query: 235 VQHVQDNRACLDLRLDAADLAGLDKAFAP 263 + ++D +L++ A + A +D P Sbjct: 285 LAQMEDYYDAAELKISAEEEALVDSLVTP 313 >gi|300313828|ref|YP_003777920.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 344 Score = 66.2 bits (160), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 59/216 (27%), Positives = 98/216 (45%), Gaps = 35/216 (16%) Query: 79 NASRAGTVKACEASLKRLHVERIDLYLLH-WRGTHPFSATIEAMETLIDQGKIGAWGVSN 137 + +R + +CE SLKR+ ++ +D++ H P T+ A+ L QGK G+S+ Sbjct: 112 SGARKHLIASCEQSLKRMGLDYVDIFYSHRVDADTPLEETMGALAHLHRQGKALYVGISS 171 Query: 138 LDNDDMHELLAL--DNGANVQVNQVLYNLSRRGIEFDLQPYCAQQGVAIMAYSPIEQG-- 193 + + A+ G + ++Q Y++ R IE L P + GV + +SP+ QG Sbjct: 172 YSPELTRKAAAILKSEGVPLLIHQPNYSMLNRAIEGGLLPALEELGVGCIGFSPLAQGLL 231 Query: 194 ----------------------RILKEA------ALVAVAKRHGVTPAQVALAWTLRHPG 225 R+L E AL +A + G T AQ+A+AW LR Sbjct: 232 TSKYLGGVPEGARVTRAPSLPQRMLSEDNLARIRALNEIASKRGQTLAQMAIAWVLRDAR 291 Query: 226 VIS-IPKASTVQHVQDNRACLD-LRLDAADLAGLDK 259 V S + A TV+ + D+ ++ L +LA +DK Sbjct: 292 VTSALIGARTVEQLDDSLDAVNKLDFTQEELASIDK 327 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.134 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 810,916 Number of extensions: 31214 Number of successful extensions: 101 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 98 Number of HSP's successfully gapped: 5 Length of query: 273 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 189 Effective length of database: 1,222,130 Effective search space: 230982570 Effective search space used: 230982570 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)