BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0150.0018.p (512 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311064|ref|YP_003775156.1| acetolactate synthase isozyme I... 193 3e-50 gi|300311743|ref|YP_003775835.1| acetolactate synthase [Herbaspi... 135 6e-33 gi|300311584|ref|YP_003775676.1| pyruvate dehydrogenase [Herbasp... 108 1e-24 gi|300310483|ref|YP_003774575.1| benzoylformate decarboxylase [H... 107 2e-24 gi|300313238|ref|YP_003777330.1| acetolactate synthase [Herbaspi... 82 1e-16 >gi|300311064|ref|YP_003775156.1| acetolactate synthase isozyme III large subunit [Herbaspirillum seropedicae SmR1] Length = 572 Score = 193 bits (491), Expect = 3e-50, Method: Compositional matrix adjust. Identities = 138/524 (26%), Positives = 244/524 (46%), Gaps = 29/524 (5%) Query: 1 LLVDALLAQDVDTTFCVPGESYLDVLNALYDHQQEIRVISCRHEGSASFMAEAYGKLTGK 60 +LV L + V+ F PG + L + +A++ Q + + I RHE +A A+AY + + K Sbjct: 11 ILVRCLAEEGVEHVFGYPGGAVLYIYDAIFK-QDKFQHILVRHEQAAVHAADAYSRSSQK 69 Query: 61 PGICFVTRGPGATNASIGVHTAFQDSTPMILFVGQVGLDMCEREAFQEIDYRRMFGPFTK 120 G+ VT GPG TNA G+ TA+ DS PM++ GQV ++AFQE D + P K Sbjct: 70 VGVAIVTSGPGVTNAVTGLATAYMDSIPMVVISGQVPSTAIGQDAFQECDTVGITRPCVK 129 Query: 121 WVTQLDDVSRVGEYVARAFSVAISGRPGPVVIALPEDMLTES---TFPR--EIGKVEPVQ 175 + DV + E V +AF +A +GRPGPV++ +P+D+ + +P+ E+ +PV Sbjct: 130 HNFLVKDVKDLAETVKKAFYIATTGRPGPVLVDIPKDITMHTCAYAYPKAVEMRSYKPVD 189 Query: 176 AYPDPDALQEMQKLLQQARRPMLILGGSNWNQDGVRNMERFASQNNLPVAASFRRQDLFD 235 + +Q LL A RPM+ GG + + + + P + + Sbjct: 190 KGHSGQIRKALQ-LLLSAERPMIYTGGGIILANAAPELNKLVDRLGYPCTNTLMGLGAYR 248 Query: 236 NRHPNYVGEVGIGVNPALAKIITESDLIIAVGSRLGEMVTSGYTLLDVPRPK------QK 289 + +VG G+ + D++IA+G+R + V + PK +K Sbjct: 249 SSSDKFVGMPGMHGTYEANMAMQHCDVLIAIGARFDDRV--------IGNPKHFASHARK 300 Query: 290 LVHVMPAAEELGRVYQTDVKILSTL-NAFARMTAQLPAVDAQ----AWRSWQKQARDAYL 344 ++H+ + + + D+ I+ + + + +QL A + + A +W KQ + Sbjct: 301 IIHIDIDPSSISKRVKVDIPIVGNVKDVLQELLSQLDAAETRQNGAALDAWWKQINEWRK 360 Query: 345 EYIEPPLRVTDF-DPALAFRQMQDILNQDAILTNGAGNYTAWAHRYYQFSQPKCQLAPTS 403 +F P +++ ++ DA +T+ G + WA +YY F +P+ + Sbjct: 361 RDCLKFATSEEFIKPQSVVQKVWEVTKGDAFITSDVGQHQMWAAQYYGFDKPRRWINSGG 420 Query: 404 -GAMGYGVPAAIAAKLCFPARQVICLAGDGCFLMTSQELTTAVAHRLGIVFLVFNNGMYA 462 G MG G+P A+ ++ P V C+ G+ M QEL T + L ++ NN Sbjct: 421 LGTMGVGLPYAMGVQMANPDATVACITGEASIQMCIQELATCKQYHLTPKIILLNNRFLG 480 Query: 463 TIRMHQEKNYPGRVMATDLNN-PDFVAYAKSFGLDACAIDKTAE 505 +R Q+ +Y R + +++ PDF A+S+G I+K + Sbjct: 481 MVRQWQQIDYGSRYSESYMDSLPDFNKLAESYGHVGMKIEKPGD 524 >gi|300311743|ref|YP_003775835.1| acetolactate synthase [Herbaspirillum seropedicae SmR1] Length = 625 Score = 135 bits (341), Expect = 6e-33, Method: Compositional matrix adjust. Identities = 133/508 (26%), Positives = 218/508 (42%), Gaps = 51/508 (10%) Query: 27 NALYDHQQEIRVISCRHEGSASFMAEAYGKLTGKPGICFVTR--GPGATNASIGVHTAFQ 84 ALY ++ + +E + + A AY K + + VT GPGATN A Sbjct: 55 EALYQYRATLPTYRAHNEQAMAHSAIAYAKAHLRQRMMAVTTSIGPGATNLLTAAALAHV 114 Query: 85 DSTPMILFVGQVGLDMCEREAFQEID--------YRRMFGPFTKWVTQLDDVSRVGEYVA 136 + P++L G V + Q+++ F P ++ ++ ++ + Sbjct: 115 NRLPVLLLPGDVFVSRRPDPVLQQLEDFNDPGLSVNDAFRPLSRMFDRICYPEQLLTALP 174 Query: 137 RAFSVAIS-GRPGPVVIALPEDMLTES-TFPREIGK---VEPVQAYPDPDALQEMQKLLQ 191 RA V + GPV +ALP+D+ T + +P + + P P L++ LL+ Sbjct: 175 RAIQVLTDPAQCGPVTLALPQDVQTMAYDYPLDFFSPRVIVPRAQAPAAQELEDAVALLR 234 Query: 192 QARRPMLILGGSNWNQDGVRNMERFASQNNLPVAASFRRQDLFDNRHPNYVGEVGIGVNP 251 QAR+P+LI GG + ++ RFA Q+ +PVA + + H +G +G+ +P Sbjct: 235 QARQPLLIAGGGVLYGNACEHLRRFAEQHGVPVAETQAGKSALPWSHALQMGAIGVTGSP 294 Query: 252 ALAKIITESDLIIAVGSRLGEMVTSGYTLLDVPRPKQKLVHVMPAAEELGRVYQTDVKIL 311 A + E+D++IAVG+RL + T +TL R V+ M A + G Q D + Sbjct: 295 AANALAQEADVVIAVGTRLQDFTTGSHTLFAQARLLNLNVNAMDAHKWRGLALQADAGL- 353 Query: 312 STLNAFARMTAQLPAVDAQAWRS---WQKQARDAYLEYIEPPLRV---TDFDPALAF--- 362 L A + + WRS W +A ++ E ++ TD L + Sbjct: 354 -GLEALSHAL--------EGWRSAPEWHARAHTLAQDWRERVAQITGQTDTGGRLPYDGE 404 Query: 363 ------RQMQDILNQDAILTNGAGNYTAWAHRYYQFSQPKC-QLAPTSGAMGYGVPAAIA 415 R + D +QD I+ AG A H+ ++ +P + MGY V + Sbjct: 405 VIGAIQRSVADSPSQD-IVVCAAGTLPAELHKLWRAGRPGAYHVEYGYSCMGYEVAGGLG 463 Query: 416 AKLCFPARQVICLAGDGCFLMTSQELTTAVAHRLGIVFLVFNNGMYATIRMHQEK--NYP 473 KL P R+VI + GDG +LM + EL T+V ++ +V +N Y I Q+ P Sbjct: 464 VKLAQPQREVIVIVGDGSYLMMNSELATSVMLGAKLIVVVLDNRGYGCINRLQQACGGAP 523 Query: 474 GRVMATDLNNP-------DFVAYAKSFG 494 M D DF A+A+S G Sbjct: 524 FNNMLADCLQAGPGAPAIDFAAHARSLG 551 >gi|300311584|ref|YP_003775676.1| pyruvate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 564 Score = 108 bits (270), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 125/511 (24%), Positives = 209/511 (40%), Gaps = 37/511 (7%) Query: 1 LLVDALLAQDVDTTFCVPGESYLDVLNALYD---HQQEIRVISCRHEGSASFMAEAYGKL 57 +L+D L V F V G D LN L D + I I RHE A+ A KL Sbjct: 8 ILIDTLYEIGVRQIFGVVG----DALNPLTDAIRRDKRIEWIGVRHEEGAALAAAGQAKL 63 Query: 58 TGKPGICFVTRGPGATNASIGVHTAFQDSTPMILFVGQVGLDMCEREAFQEIDYRRMF-- 115 TG+ +C T GPGA + G++ A +D P++ G V + QE +F Sbjct: 64 TGRLAVCCGTTGPGANHLVAGLYEARKDHAPVLAISGGVPAAHRGTDYLQENSPELLFRD 123 Query: 116 -GPFTKWVTQLDDVSRV-GEYVARAFSVAISGRPGPVVIALPEDML---TESTFPREIGK 170 +++ +T D V + +A+A++ + G + +P D++ T + P Sbjct: 124 VAAYSQIITSPDQAPAVFHQAIAQAYA-----QRGVAHLNIPADVIGAKTSGSVPSLHTL 178 Query: 171 VEPVQAYPDPDALQEMQKLLQQARRPMLILGGSNWNQDGVRNMERFASQNNLPVAASFRR 230 E P +Q L+ A + G N + + + AS+ PV +FR Sbjct: 179 RERTPLSPPAADIQAAADLINAAGSVAIFAG--NGCRGAIDEVLALASKLQAPVMHTFRG 236 Query: 231 QDLFDNRHPNYVGEVG-IGVNPALAKIITESDLIIAVGSRLGEMVTSGYTLLDVPRPKQK 289 +DL HP+++G VG IG P +D + A L M+ S Y + K K Sbjct: 237 KDLASYDHPHWIGGVGLIGGAPG-------TDALHAAEVML--MLGSDYPYSEFLPTKSK 287 Query: 290 LVHVMPAAEELGRVYQTDVKILSTLN-AFARMTAQLPAVDAQAWRSWQKQARDAYLEYIE 348 ++ + LGR + I ++ A ++ ++ A+ R+ + ++ Sbjct: 288 VIQIDERGFVLGRRAPVTLGITGSVAPAVQQLLERVEGKTDGAFLQQANAHRERWNAKLD 347 Query: 349 PPLRVT----DFDPALAFRQMQDILNQDAILTNGAGNYTAWAHRYYQFSQPKCQLAP-TS 403 + P R + D+ + DA G T W + + + LA + Sbjct: 348 SAAEIKRHHKKIKPQSVARAISDLADDDAAFVVDTGVVTLWCGNWIRQRGGQRILASFNN 407 Query: 404 GAMGYGVPAAIAAKLCFPARQVICLAGDGCFLMTSQELTTAVAHRLGIVFLVFNNGMYAT 463 A+G + A + RQV+ GDG F M E T+V HRL + +VFNN + Sbjct: 408 AAVGTSLGQANGIQALDRERQVVVAVGDGGFTMLLGEFMTSVEHRLPVKVVVFNNREWGL 467 Query: 464 IRMHQEKNYPGRVMATDLNNPDFVAYAKSFG 494 + + E+ ++ NPDF A+A++ G Sbjct: 468 VHLEMEEAGMPAFEGSEFPNPDFAAFARACG 498 >gi|300310483|ref|YP_003774575.1| benzoylformate decarboxylase [Herbaspirillum seropedicae SmR1] Length = 532 Score = 107 bits (268), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 131/536 (24%), Positives = 225/536 (41%), Gaps = 64/536 (11%) Query: 2 LVDALLAQDVDTTFCVPGESYLDVLNALYDHQQEIRVISCRHEGSASFMAEAYGKLTGKP 61 ++D + + + F PG + L + D ++ R + E MA+ + + +G Sbjct: 15 VLDLMRRLGMTSVFANPGSTELPMFR---DFPEDFRYVLGLQEAVVVGMADGFAQASGNA 71 Query: 62 GICFVTRGPGATNASIGVHTAFQDSTPMILFVGQVGLDMCEREAF-QEIDYRRMFGPFTK 120 + + G NA + TAF++ TP+I+ GQ + + F + P+ K Sbjct: 72 ALVNLHSAAGVGNAMGNIFTAFRNRTPLIITAGQQARSILPFDPFLGATQATELAKPYLK 131 Query: 121 WVTQLDDVSRVGEYVARAFSVAISGRPGPVVIALPEDMLTESTFPREIGKVEPVQAY--- 177 W + + V +ARA+ +A+ GPV +++P D + P E+ V P Q Sbjct: 132 WSIEPARAADVPLALARAYHMAMQEPRGPVFVSIPVDDWDQ---PAEL--VPPTQVTSRC 186 Query: 178 -PDPDALQEMQKLLQQARRPMLILGG-----SNWNQDGVRNMERFASQNNLPVAASFRRQ 231 P+P A+ + L+Q++RP +++G W+ + E ++ + A + Sbjct: 187 RPEPAAIARLAAALEQSQRPAIVVGAELDRAGAWDA-AIALAETLRARVFV---APMSSR 242 Query: 232 DLFDNRHPNYVGEVGIGVNPALAKIITES----DLIIAVGSRL--------GEMVTSG-- 277 F HP + G + PA+ + I ++ DL+ A+G+ G V G Sbjct: 243 CSFPEDHPLFAGFL-----PAMREKIVQALQGHDLLFALGAPAFTYHVEGSGPHVPPGAA 297 Query: 278 -YTLLDVPRPKQKLVHVMPAAEELGRVYQTDVKILSTLNAFARMTAQLPAVDAQAWRSWQ 336 + L+D P V + G+ +++ L + A++ Q P AQ Sbjct: 298 LFQLIDDP--------VTASWTPTGQAIVGNIQ-LGLDDLLAQIRRQPPTQRAQ---PPA 345 Query: 337 KQARDAYLEYIEPPLRVTDFDPALAFRQMQDILNQDAILTNGAGNYTAWAHRYYQFSQPK 396 + AR A +E P A A + + D+ I+ A + F++P Sbjct: 346 RPAR-ARVEASAP------LSMAYALQTLADVREAGDIVVEEAPGSRPVMQAHLPFTRPG 398 Query: 397 CQLAPTSGAMGYGVPAAIAAKLCFPARQVICLAGDGCFLMTSQELTTAVAHRLGIVFLVF 456 SG +GYG+PAA+ L P +VI L GDG + + Q + +AV L I F++ Sbjct: 399 SFYTMASGGLGYGMPAAVGVALARPHSRVIGLIGDGSAMYSPQAIYSAVQLGLPITFVIL 458 Query: 457 NNGMYATIRMHQEKNYPG---RVMATDLNNPDFVAYAKSFGLDACAIDKTAEFAPA 509 NNG YA ++ G V T+L DFVA A S G + + A APA Sbjct: 459 NNGRYAALQDFAPVFGFGPEDPVHGTELPGLDFVALAHSMGCHGVRVSQAAALAPA 514 >gi|300313238|ref|YP_003777330.1| acetolactate synthase [Herbaspirillum seropedicae SmR1] Length = 550 Score = 81.6 bits (200), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 124/518 (23%), Positives = 207/518 (39%), Gaps = 68/518 (13%) Query: 6 LLAQDVDTTFCVPGESYLDVLNALYDHQQEIRVISCRHEGSASFMAEAYGKLTGKPGICF 65 LL Q VDT F PG + LD++ L + ++R + E + A+ YG++ G+P Sbjct: 46 LLDQGVDTCFANPGTTELDLVRGL-ESFPKLRCVVALQENVCTGAADGYGRMLGRPASTL 104 Query: 66 VTRGPGATNASIGVHTAFQDSTPMILFVGQVGLDMCEREAFQEIDYRRMFGPFTKWVTQL 125 + GPG N +H A + TP++ +G +A D + P + WV ++ Sbjct: 105 LHLGPGFANGIANLHNARRARTPIVNIIGDHTSWHLPYDAPLSSDIESLAHPVSGWVHRI 164 Query: 126 DDVSRVGEYVARAF--SVAISGRPGPVVIALPEDM----LTEST----FPREIGKVEPVQ 175 + + A + S A G+ G +I P D + E++ ++ G+ + + Sbjct: 165 TGTADIAAAAAESVRQSTAAGGQ-GSTLI-FPADFQYADMAEASPAAATEKDTGQADDYR 222 Query: 176 AYPDPDALQEMQKLLQQARRPMLILGGSNWNQDGVRNMERFASQNNLPV------AASFR 229 A LQ Q+++ G Q R + R A+ V A + R Sbjct: 223 ADQALARLQGAQRVVFLLGGGGEESGLGARAQ---RAVARIAAHIGCSVYAETFPARAER 279 Query: 230 RQDL--FDNRHPNYVGEVGIGVNPALAKIITESDLIIAVGSRLGEMVTSGYT--LLDVPR 285 Q L FD R P + PA K++ ++DL++ VG+ L + GY + Sbjct: 280 GQGLPSFD-RLPYFP-------EPAR-KVLDDADLVVLVGA-LPPITYFGYVGHPSALVA 329 Query: 286 PKQKLVHVMPAAEELGRVYQTDVKILSTLNAFARMTAQLPAVDAQAWRSWQKQARDAYLE 345 P++ L +P GRV Q + L A A +T Q Sbjct: 330 PERLLTISLPGRPAAGRVEQ----LADALGAPAYVTPQ---------------------- 363 Query: 346 YIEPPLRVTDFDPALAFRQMQDILNQDAILTNGAGNYTAWAHRYYQFSQPKCQLAPTSGA 405 + PPL + P + L + AI++ G + ++P L T GA Sbjct: 364 HALPPLPEGELTPQKIAAVLARELPERAIVSVEGGTCGYPFYAVSDAARPHTVLTNTGGA 423 Query: 406 MGYGVPAAIAAKLCFPARQVICLAGDGCFLMTSQELTTAVAHRLGIVFLVFNNGMYATIR 465 +G G+P A+ A + P R V+ L DG T Q L + +V L+ N YA +R Sbjct: 424 IGQGLPVALGAAVACPDRTVVGLLSDGSTQYTIQTLWSLAHENARVVVLIAANHQYAILR 483 Query: 466 MHQEKN------YPGRVMATDLNNPDFVAYAKSFGLDA 497 ++ ++ A D D+V A+S+G+ A Sbjct: 484 NELRRDGAPLGERAAQMTALDHPRIDWVGLAQSYGVPA 521 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,660,524 Number of extensions: 72919 Number of successful extensions: 198 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 185 Number of HSP's successfully gapped: 6 Length of query: 512 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 423 Effective length of database: 1,198,455 Effective search space: 506946465 Effective search space used: 506946465 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)