BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0149.0012.p (360 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309787|ref|YP_003773879.1| sugar (pentulose and hexulose) ... 508 e-145 gi|300312696|ref|YP_003776788.1| D-xylulose kinase [Herbaspirill... 328 4e-91 gi|300310425|ref|YP_003774517.1| gluconokinase [Herbaspirillum s... 83 3e-17 gi|300310306|ref|YP_003774398.1| glycerol kinase [Herbaspirillum... 67 2e-12 gi|300310399|ref|YP_003774491.1| D-ribulose/ribitol kinase [Herb... 58 1e-09 >gi|300309787|ref|YP_003773879.1| sugar (pentulose and hexulose) kinase [Herbaspirillum seropedicae SmR1] Length = 490 Score = 508 bits (1309), Expect = e-145, Method: Compositional matrix adjust. Identities = 264/374 (70%), Positives = 287/374 (76%), Gaps = 40/374 (10%) Query: 1 MYLGIDLGTSEVKAVVIDAEGKLLALAGSPLTVSRPHPRWSEQDPQHWWRAACDTVASLR 60 MYLGIDLGTSEVKAVVIDA+G L+ALAGS L V+RPHPRWSEQ P WW+A CDTVA LR Sbjct: 1 MYLGIDLGTSEVKAVVIDAQGSLVALAGSTLNVARPHPRWSEQAPADWWQATCDTVAKLR 60 Query: 61 EQLGAARFAAIRAIGLSGQMHGAVLLDAQERVLRPAILWSDSRSTDECAELTRRWNESGD 120 QLG+ RF +IRAIGLSGQMHGAVLLD ++ VLRPAILWSDSRS ECAEL R + Sbjct: 61 TQLGSERFGSIRAIGLSGQMHGAVLLDERDEVLRPAILWSDSRSAPECAELESR---APR 117 Query: 121 LHQLAGNLAMPGFTAPKLLWVARHEPQLFDQVACVLLPKDWLRLRMTGRKTSDPSDAAGT 180 LH +AGNLAMPGFTAPKLLWVARHEPQLF ++A VLLPKDWLRL+MTGRK SDPSDAAGT Sbjct: 118 LHGIAGNLAMPGFTAPKLLWVARHEPQLFARIATVLLPKDWLRLKMTGRKVSDPSDAAGT 177 Query: 181 LWLDVAQRDWSDALLAAGGMHRGQMPELADGNAAAGFLLPEVARAWGLADGVVVAG---- 236 LWLDV R+WSD LLAA GM R QMP L DG+A +G LLPEVA+AWGL V+VAG Sbjct: 178 LWLDVEGRNWSDELLAASGMRRDQMPALVDGSAVSGSLLPEVAQAWGLRSDVIVAGGAGD 237 Query: 237 --------------------GAGGX-------------RAIHAFCHTLPQRWHQMSVMLS 263 G G RAIHAFCHTLPQRWHQMSVMLS Sbjct: 238 GAASAVGIGAVKPGDGFLSLGTSGVLFVVNDRFRPNPGRAIHAFCHTLPQRWHQMSVMLS 297 Query: 264 AASCLRWFCRLCSVDEKTLLQEVAALDDEALANAPLFLPYLSGERTPHNDPYALGVFHGL 323 AASCLRWFCRLCSVDEK+LL E+ LD++A NAPLFLPYLSGERTPHNDPYA GVFHGL Sbjct: 298 AASCLRWFCRLCSVDEKSLLAEIEQLDEQACNNAPLFLPYLSGERTPHNDPYATGVFHGL 357 Query: 324 SHEHQRAALAYAVL 337 + EHQRAAL YAVL Sbjct: 358 TPEHQRAALGYAVL 371 >gi|300312696|ref|YP_003776788.1| D-xylulose kinase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 328 bits (841), Expect = 4e-91, Method: Compositional matrix adjust. Identities = 182/382 (47%), Positives = 227/382 (59%), Gaps = 41/382 (10%) Query: 2 YLGIDLGTSEVKAVVIDAEGKLLALAGSPLTVSRPHPRWSEQDPQHWWRAACDTVASLRE 61 YLGIDLGTSEVK V+ D + ++A + L V PHP WSEQ PQ WW A D +A+LR Sbjct: 3 YLGIDLGTSEVKLVLTDEDSNVIASTSARLRVDNPHPLWSEQAPQSWWNATLDAIAALRT 62 Query: 62 QLGAARFAAIRAIGLSGQMHGAVLLDAQERVLRPAILWSDSRSTDECAELTRRWNESGDL 121 Q A F A+R IGLSGQMHGA LLD VLRPAILW+D R+ EC EL D Sbjct: 63 QAPQA-FQALRGIGLSGQMHGATLLDRNGNVLRPAILWNDMRAHAECVELEAL---VPDA 118 Query: 122 HQLAGNLAMPGFTAPKLLWVARHEPQLFDQVACVLLPKDWLRLRMTGRKTSDPSDAAGTL 181 + GN AMPGFTAPKLLW++++EP ++ + VLLPKD+L ++TG S+ SDAAGTL Sbjct: 119 VAITGNRAMPGFTAPKLLWLSKYEPAVYRAIDKVLLPKDFLGWKLTGEFVSEMSDAAGTL 178 Query: 182 WLDVAQRDWSDALLAAGGMHRGQMPELADGNAAAGFLLPEVARAWGLADGVVVAGGAG-- 239 WLDVA+RDWS+ +L A G+ R MP L +G+A AG L E+ R WG++ VVVAGGAG Sbjct: 179 WLDVARRDWSERMLGATGLDRSHMPRLVEGSAVAGQLRDELRREWGISGPVVVAGGAGDN 238 Query: 240 -----------------------------------GXRAIHAFCHTLPQRWHQMSVMLSA 264 + +HAFCH LP +WHQMSV+LSA Sbjct: 239 AASAVGIGVIRAGDAFLSLGSSGVLFAATAHHAPNAQQGVHAFCHCLPGQWHQMSVILSA 298 Query: 265 ASCLRWFCRLCSVDEKTLLQEVAALDDEALANAPLFLPYLSGERTPHNDPYALGVFHGLS 324 A+ L W + + L+ L A APLFLPYL+GERTPHND A GV G++ Sbjct: 299 AASLHWLSGVTARPVAELVSGAERLSAGQQAQAPLFLPYLNGERTPHNDAAAKGVLFGMT 358 Query: 325 HEHQRAALAYAVLXKKPIPASD 346 H+ A LAYAV+ +D Sbjct: 359 PAHESAHLAYAVMEGVAFAMAD 380 >gi|300310425|ref|YP_003774517.1| gluconokinase [Herbaspirillum seropedicae SmR1] Length = 520 Score = 83.2 bits (204), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 96/382 (25%), Positives = 157/382 (41%), Gaps = 50/382 (13%) Query: 3 LGIDLGTSEVKAVVIDAEGKLLALAGSPLTVSRPHPRWSEQDPQHWWRAACDTVASLREQ 62 LG+D+GT+ K+VV +GK++A V P +EQDP+ AA ++A + Sbjct: 11 LGVDIGTTSTKSVVFTLDGKVVAQHAEEYPVLCTEPGMAEQDPEQIVAAALASIAGAVKA 70 Query: 63 LGAARFAAIRAIGLSGQMHGAVLLDAQERVLRPAILWSDSRSTDECAELTRRWNESGDLH 122 AA I + S MH + LDA+ R+L +I W D R++ AE + ++ ++ Sbjct: 71 AKAAP-QEIALLSFSAAMHSVIALDAENRLLSNSITWGDIRAS-VWAERIKHEHDGNAIY 128 Query: 123 QLAGNLAMPGFTAPKLLWVARHEPQLFDQVACVLLPKDWLRLRMTGRKTSDPSDAAGTLW 182 + G P KL+W+ +P +F + A + K++L ++ G+ D S A+ T Sbjct: 129 RRTGTPVHPMSPLCKLMWMRHEKPDVFHRAARFVGIKEYLLYQLFGQWVVDHSIASATGM 188 Query: 183 LDVAQRDWSDALLAAGGMHRGQMPELADGNAAAGFLLPEVARAWGLA----------DGV 232 ++ + W LA G+ Q+P L E+A+ GL+ DGV Sbjct: 189 FNLRELAWDQGALALLGVRPDQLPTPVPTTHRLPALSSEMAQRLGLSVDTPFIIGANDGV 248 Query: 233 ---------------VVAGGAGGXRAI----------HAFCHTLPQR-WHQMSVMLSAAS 266 V G +G R + FC+ L ++ W + + + Sbjct: 249 LSNLGVNAIEIGHVAVTIGTSGAMRTVIDEPRTDPQGRLFCYALTEKHWVVGGPVNNGGN 308 Query: 267 CLRWF-----------CRLCSVDEKTLLQEVAALDDEALANAPLFLPYLSGERTPHNDPY 315 RW R VD L +A A LF P+++GER P + Sbjct: 309 IFRWVRDELATAEAAAAREEGVDPYEALTRIAEKVRPG-AEGLLFHPFMAGERAPLWNAD 367 Query: 316 ALGVFHGLSHEHQRAALAYAVL 337 G F GL+ H + + A L Sbjct: 368 LRGSFFGLALHHGKHHMIRAAL 389 >gi|300310306|ref|YP_003774398.1| glycerol kinase [Herbaspirillum seropedicae SmR1] Length = 495 Score = 67.4 bits (163), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 55/213 (25%), Positives = 95/213 (44%), Gaps = 9/213 (4%) Query: 3 LGIDLGTSEVKAVVIDAEGKLLALAGSPLTVSRPHPRWSEQDPQHWWRAACDTVASLREQ 62 L +D GTS ++++ D G ++A A P P W E DP W + T + Q Sbjct: 5 LALDQGTSSSRSIIFDQSGAIVATAQQEFPQIFPQPGWVEHDPMDLWHSQLQTAQKVLAQ 64 Query: 63 LGAARFAAIRAIGLSGQMHGAVLLD-AQERVLRPAILWSDSRSTDECAELTRRWNESGDL 121 G + + + A+G++ Q V+ + A + + A++W D R+ CA L R + Sbjct: 65 SG-IKASELTALGITNQRETTVVWERATGKPVYNAVVWQDRRAEVVCAALRER-GLGEAI 122 Query: 122 HQLAGNLAMPGFTAPKLLWVARHEPQLFDQVACVLL----PKDWLRLRMTGRK--TSDPS 175 + G + P F+ KL W+ H L + L WL +TG + SD S Sbjct: 123 QKKTGLVLDPYFSGTKLRWILDHVDGLRARAERGELLFGTVDSWLVWNLTGGRLHVSDVS 182 Query: 176 DAAGTLWLDVAQRDWSDALLAAGGMHRGQMPEL 208 +A+ T+ ++ W + LL G+ +P++ Sbjct: 183 NASRTMLWNIHTGAWDEELLQWLGIPAAMLPQV 215 >gi|300310399|ref|YP_003774491.1| D-ribulose/ribitol kinase [Herbaspirillum seropedicae SmR1] Length = 548 Score = 57.8 bits (138), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 69/251 (27%), Positives = 100/251 (39%), Gaps = 22/251 (8%) Query: 3 LGIDLGTSEVKAVVIDAEGKLLALAGSPLTVSRPHPRWS-EQDPQHWWRAACDTVASLRE 61 +GID+GT +A V D +G+LLA A + + R R EQ W A C V + Sbjct: 6 IGIDVGTGSARAGVFDPQGQLLASARHDIDLFRDERRARVEQSSAQIWEAVCQAVRAAVT 65 Query: 62 QLGAARFAAIRAIGLSGQMHGAVLLDAQERVLRPA------ILWSDSRSTDECAELTRRW 115 + G AA+ IG+ V+ A V P I+W D R+ ++ + R Sbjct: 66 KSG-IDPAAVAGIGVDATCS-LVVQGASAGVGDPGHPERDVIVWMDHRALEQAQRINRMG 123 Query: 116 NESGDLHQLAGNLAMPGFTAPKLLWVARHEPQLFDQVACVLLPKDWLRLRMTGRKTSDPS 175 + + G + P PKLLW+ + P+++ A D+L + TG S Sbjct: 124 HA---VLSYVGGVISPEMETPKLLWLKENLPEVYASAAHFFDLTDFLTWKATG-SLQRSS 179 Query: 176 DAAGTLWLDVAQRDWSDAL---------LAAGGMHRGQMPELADGNAAAGFLLPEVARAW 226 W +A DA LA G R + G AG L A+A Sbjct: 180 CTVTCKWTYLAHEAGWDASYFRQIGLGDLAEAGFARIGAEVVWPGTPLAGGLTQTAAQAM 239 Query: 227 GLADGVVVAGG 237 GL G+ VA G Sbjct: 240 GLRPGIAVAAG 250 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.133 0.430 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,145,060 Number of extensions: 45253 Number of successful extensions: 158 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 149 Number of HSP's successfully gapped: 5 Length of query: 360 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 274 Effective length of database: 1,212,660 Effective search space: 332268840 Effective search space used: 332268840 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)