BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0149.0005.p (316 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309782|ref|YP_003773874.1| sugar ABC transporter permease ... 545 e-156 gi|300313088|ref|YP_003777180.1| sn-glycerol-3-phosphate ABC tra... 109 3e-25 gi|300309613|ref|YP_003773705.1| sugar ABC transporter permease ... 94 2e-20 gi|300311926|ref|YP_003776018.1| glycerol-3-phosphate ABC transp... 89 6e-19 gi|300310302|ref|YP_003774394.1| sugar ABC transporter permease ... 86 4e-18 >gi|300309782|ref|YP_003773874.1| sugar ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 324 Score = 545 bits (1405), Expect = e-156, Method: Compositional matrix adjust. Identities = 267/306 (87%), Positives = 284/306 (92%) Query: 11 SSQKSRRGKHIKHAGSERFLPIRLLQTPAVALLFLWMIVPLAMTVYFSFNRYNLMSPDQT 70 +SQ + R + ERF PIRL+QTP+V +L WMIVPLAMT+YFS NRYNLM+PDQT Sbjct: 19 TSQPASRRQQTSPPRKERFNPIRLMQTPSVLILLAWMIVPLAMTIYFSVNRYNLMAPDQT 78 Query: 71 GFIGLENYQFLLEDPAFWPSIANTALLIGSVLGISVIGGTLLAVLFDQPFRGRGIARLLV 130 GF+GLENY FLL+DPAFWPSI NTALLIGSVL ISVIGGTLLAVLFDQPFRGRGIARLLV Sbjct: 79 GFVGLENYAFLLQDPAFWPSIGNTALLIGSVLAISVIGGTLLAVLFDQPFRGRGIARLLV 138 Query: 131 IGPFFVMPTVAALIWKNMIMHPVYGMLAFAMRHLGMEPIDWLAEYPMLSVIIIVSWQWIP 190 IGPFFVMPTVAALIWKNMIMHPVYG+LA+ MRHLG+EPIDWLAEYPMLSVIIIV+WQWIP Sbjct: 139 IGPFFVMPTVAALIWKNMIMHPVYGILAYVMRHLGLEPIDWLAEYPMLSVIIIVAWQWIP 198 Query: 191 FAFLILLTALQSLDVDQKEAAQLDGAGPIRIFFYIVLPHLKRAITVVIMIETIFLLSVFA 250 FAFLILLTALQSLD DQKEAAQLDGAGP+RIFFYIVLPHLKRAITVVIMIETIFLLSVFA Sbjct: 199 FAFLILLTALQSLDTDQKEAAQLDGAGPVRIFFYIVLPHLKRAITVVIMIETIFLLSVFA 258 Query: 251 EIFTTTAGGPGTETTNLTYLVYSIGLQQFDIGIASAGGIFAVVLANIVSFFLVRMLAKNL 310 EIFTTTAGGPGT TTNLT+LVYSIGLQQFDIGIASAGGIFAVVLANIVSFFLVRMLA+NL Sbjct: 259 EIFTTTAGGPGTATTNLTFLVYSIGLQQFDIGIASAGGIFAVVLANIVSFFLVRMLARNL 318 Query: 311 KGANEK 316 KG NEK Sbjct: 319 KGVNEK 324 >gi|300313088|ref|YP_003777180.1| sn-glycerol-3-phosphate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 294 Score = 109 bits (273), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 83/295 (28%), Positives = 145/295 (49%), Gaps = 15/295 (5%) Query: 26 SERFLPIRLLQTPAVALLFLWMIVPLAMTVYFSFNRYNLMSPDQTG----FIGLENYQFL 81 + ++LP LL P + + L+ P +Y S ++ D G F+ +N++ L Sbjct: 8 TSKWLP-YLLVAPQIIITLLFFFWPAVQALYQS-----MLLQDAFGGYSEFVWFDNFKTL 61 Query: 82 LEDPAFWPSIANTALLIGSVLGISVIGGTLLAVLFDQPFRGRGIARLLVIGPFFVMPTVA 141 DP + S TA+ V + LLAV D+ RG I + +I P+ V P V Sbjct: 62 FGDPTYLESFRTTAIFSALVAFFGLALSLLLAVFADRVRRGASIYKTFLIWPYAVSPVVV 121 Query: 142 ALIWKNMIMHPVYGMLAFAMRHLGMEPIDWL--AEYPMLSVIIIVSWQWIPFAFLILLTA 199 ++W ++ P G+L+ + LG+ P D++ + M+ ++I W+ I + FL L Sbjct: 122 GVLWM-FLLSPSLGILSHVLSWLGL-PWDYMINGTHAMILIVIAAIWKQISYNFLFFLAG 179 Query: 200 LQSLDVDQKEAAQLDGAGPIRIFFYIVLPHLKRAITVVIMIETIF-LLSVFAEIFTTTAG 258 LQS+ EAA +DGAGP++ FF IV P + + ++ ++ F + TT G Sbjct: 180 LQSIPKSLIEAAAIDGAGPVKRFFTIVFPLISPTTFFLFVVNVVYAFFDTFVIVDATTHG 239 Query: 259 GPGTETTNLTYLVYSIGLQQFDIGIASAGGIFAVVLANIVSFFLVRMLAKNLKGA 313 GPG +T L Y V+S G + D+G ++A + + L +++ + + K ++ A Sbjct: 240 GPGKDTEILVYKVFSDGFKGGDLGGSAAQSVVLMALVILLTVVQFKYVEKKVQYA 294 >gi|300309613|ref|YP_003773705.1| sugar ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 311 Score = 93.6 bits (231), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 75/271 (27%), Positives = 134/271 (49%), Gaps = 9/271 (3%) Query: 38 PAVALLFLWMIVPLAMTVYFSFNRYNLMSPDQTGFIGLENYQFLLEDPAFWPSIANTALL 97 PA + ++++ P+ T+Y SF ++ M+ + F+GL NY L P F+ ++ N L Sbjct: 44 PACVMTLVYVLYPILYTIYLSFFSWDGMTEPR--FVGLANYVELFHAPTFYTALKNNVLW 101 Query: 98 IGSVLGISVIGGTLLAVLFDQPFRGRGIARLLVIGPFFVMPTVAALIWKNMIMHPVYGML 157 + L ++ G +A+ +Q G + + L PF + V L++ + P +G+L Sbjct: 102 LLMFL-LAPPMGLAIALYLNQKVIGMRVVKSLFFAPFVLSGVVVGLMY-SWFYDPSFGLL 159 Query: 158 AFAMRHLGMEPIDWLAEYPMLSVIIIVSWQWIPFAFLILLTALQSLDVDQKEAAQLDGAG 217 + G+ P+ A Y +I W + ++ LT L +L+ DQ EAA+++GA Sbjct: 160 SLLFGQ-GV-PVLGDARYATFGIIFAALWPQTAYCMILFLTGLTALNHDQVEAARMEGAK 217 Query: 218 PIRIFFYIVLPHLKRAITVVIMIETIFLLSVFAEIFTTTAGGPGTETTNLTYLVYSIGLQ 277 + Y++LP L+ + ++ I L F I T+GGP +T L Y Y ++ Sbjct: 218 GWTMLRYVILPQLRSTTFMAFVVSIIGALRSFDLISVMTSGGPYESSTVLAYYAYDQAIK 277 Query: 278 QFDIGIASAGGI--FAVVLANIVSFFLVRML 306 + G ++A + FA++L IV + L RML Sbjct: 278 YYRQGYSAAVAVTLFAIMLVYIV-YQLRRML 307 >gi|300311926|ref|YP_003776018.1| glycerol-3-phosphate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 294 Score = 88.6 bits (218), Expect = 6e-19, Method: Compositional matrix adjust. Identities = 83/274 (30%), Positives = 136/274 (49%), Gaps = 19/274 (6%) Query: 22 KHAGSERFLPIRLLQTPAVALLFLWMIVPLAMTVYFSFNRYNLMSPDQTG--FIGLENYQ 79 + A R+ P+ LL TP + ++FL+ + P + +SF L+ P G F+GL+NY Sbjct: 4 RPAFPTRWFPL-LLATPQMLIVFLFFLWPAVKAIAWSFY---LVRPFGNGSRFVGLDNYL 59 Query: 80 FLLEDPAFWPSIANTALLIG--SVLGISVIGGTLLAVLFDQPFRGRGIARLLVIGPFFVM 137 +L D +F+ S+ T + ++L +++ + D R + + R + I P+ V Sbjct: 60 RILTDDSFYASLRATLVFTAGSTLLAVTIALALAACLELD--VRAKRLLRSIFIWPYAVA 117 Query: 138 PTVAALIWKNMIMHPVYGMLAFAMRHLGMEPIDW----LAEYPMLSVIIIVSWQWIPFAF 193 V ++ K +I +PV G+LAF + + P W + ML++I +W +P F Sbjct: 118 GVVVGIVLKVLI-NPVTGLLAFLNQ---LWPGVWAPHLIGAQAMLALIAAFAWTQVPLNF 173 Query: 194 LILLTALQSLDVDQKEAAQLDGAGPIRIFFYIVLPHLKRAITVVIMIETIFLLSV-FAEI 252 ++ ALQ + D AA +DGAGP R F I LP + A+ + +I I + F + Sbjct: 174 ILFSAALQRIPADLMGAAAIDGAGPWRRFIDIQLPMIAPALLLAGVINVIEAFTHGFGLV 233 Query: 253 FTTTAGGPGTETTNLTYLVYSIGLQQFDIGIASA 286 T GGPG T L Y +YS G D+ +SA Sbjct: 234 DALTQGGPGRSTAILAYQIYSDGFIGLDLSGSSA 267 >gi|300310302|ref|YP_003774394.1| sugar ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 296 Score = 85.9 bits (211), Expect = 4e-18, Method: Compositional matrix adjust. Identities = 71/275 (25%), Positives = 131/275 (47%), Gaps = 18/275 (6%) Query: 48 IVPLAMTVYFSFNRYNLMSPDQTGFIGLENYQFLLEDPAFWPSIANTALLIGSVLGISVI 107 I+PL V +S +++SP+Q F+GL+ ++ ++ D ++ + VL + + Sbjct: 25 IIPLMTVVNYSVQ--DIISPEQKVFVGLDWFREVMRDGELHAALLRQLIFSFCVLLVQIP 82 Query: 108 GGTLLAVLFDQPFRGRGIARLLVI--GPFFVMPTVAALIWKNMIMHPVYGMLAFAMRHLG 165 G LA+ P +G + LVI P + V IW+ + G+ + + LG Sbjct: 83 LGIGLALAM--PHKGWKSSATLVILAMPLLIPWNVVGTIWQ-IFGRADIGLGGYVLNLLG 139 Query: 166 ME------PIDWLAEYPMLSVIIIVSWQWIPFAFLILLTALQSLDVDQKEAAQLDGAGPI 219 ++ P+D L+V+++ W W P L+ L+S+ +AAQ+DGA Sbjct: 140 IDYNYASNPLD-----AWLTVLLMDVWHWTPLVALLAFAGLRSIPDAYYQAAQIDGASRW 194 Query: 220 RIFFYIVLPHLKRAITVVIMIETIFLLSVFAEIFTTTAGGPGTETTNLTYLVYSIGLQQF 279 +F YI LP ++ + + +++ + ++ E F T GGPG TT L+ + + QF Sbjct: 195 AVFRYIQLPKMRGVLVIAVLLRFMDSFMIYTEPFVLTGGGPGNSTTFLSQYLTQKAVGQF 254 Query: 280 DIGIASAGGIFAVVLANIVSFFLVRMLAKNLKGAN 314 D+G A+A + ++ ++SF L + AN Sbjct: 255 DLGPAAAFSLIYFLIILLLSFVLYNWMQSAGNHAN 289 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.143 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 885,950 Number of extensions: 33482 Number of successful extensions: 178 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 161 Number of HSP's successfully gapped: 11 Length of query: 316 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 231 Effective length of database: 1,217,395 Effective search space: 281218245 Effective search space used: 281218245 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 130 (54.7 bits)