BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0148.0052.p (147 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309502|ref|YP_003773594.1| preprotein translocase subunit ... 264 2e-72 >gi|300309502|ref|YP_003773594.1| preprotein translocase subunit SecB protein [Herbaspirillum seropedicae SmR1] Length = 164 Score = 264 bits (675), Expect = 2e-72, Method: Compositional matrix adjust. Identities = 125/138 (90%), Positives = 137/138 (99%) Query: 1 MAEENQQAEQQPVFQIQRVYLKDLSLEQPNSPAIFLEQDAPAIEVAVDVGAEALADGIFE 60 M+E+NQ+A+QQPVFQIQRVYLKDLSLEQPNSPAIFLEQ+AP+IEV+VDVGAEALA+G+FE Sbjct: 1 MSEQNQEAQQQPVFQIQRVYLKDLSLEQPNSPAIFLEQEAPSIEVSVDVGAEALAEGVFE 60 Query: 61 STVTITVTAKVKDKVAFLVEGKQAGIFEVRNIPAEQLDPLLGIGCPNIIYPYLRANIADV 120 STVTITVTAKVKDKVAFLVEGKQAGIFE+RN+PAEQLDPLLGIGCPNIIYPYLRANIAD Sbjct: 61 STVTITVTAKVKDKVAFLVEGKQAGIFEIRNVPAEQLDPLLGIGCPNIIYPYLRANIADA 120 Query: 121 ITRAGFPPIHLAEINFEV 138 ITRAGFPP+HLAEINFEV Sbjct: 121 ITRAGFPPVHLAEINFEV 138 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 432,478 Number of extensions: 16599 Number of successful extensions: 40 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 40 Number of HSP's successfully gapped: 1 Length of query: 147 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 70 Effective length of database: 1,255,275 Effective search space: 87869250 Effective search space used: 87869250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)