BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0148.0043.p (150 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309511|ref|YP_003773603.1| hypothetical protein Hsero_0169... 98 3e-22 >gi|300309511|ref|YP_003773603.1| hypothetical protein Hsero_0169 [Herbaspirillum seropedicae SmR1] Length = 142 Score = 98.2 bits (243), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 66/143 (46%), Positives = 78/143 (54%), Gaps = 17/143 (11%) Query: 20 RRRRSGHM-GLALALAVMLLFSQWLGLLHGIAHAGWPEQASASPVASFNAALFNYVDDD- 77 R R H L LA+ LL +Q LG +HGIAHAGW A + + + ALF+ + D Sbjct: 5 HRLRCLHTPALVAILAMSLLLAQMLGFVHGIAHAGW---APGTVHSLISDALFDEGEGDD 61 Query: 78 ----------ADVNADDGKLPAAHGKPHHSCAEYDAAAGAAGIHARFFSPPLIPGTQVLA 127 D + A H HHSC YDAAA +AG H F PL+P +VLA Sbjct: 62 HAASAHVHGGQQQGEDHAHVHADHS--HHSCVSYDAAALSAGAHFDFPPLPLMPPVRVLA 119 Query: 128 LWQAFASWDAPAVCHFSSRAPPR 150 LWQAFASWD P CHFSSRAPPR Sbjct: 120 LWQAFASWDPPLNCHFSSRAPPR 142 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.134 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 445,816 Number of extensions: 14288 Number of successful extensions: 63 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 62 Number of HSP's successfully gapped: 1 Length of query: 150 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 73 Effective length of database: 1,255,275 Effective search space: 91635075 Effective search space used: 91635075 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 126 (53.1 bits)