BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0148.0040.p (211 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309516|ref|YP_003773608.1| branched-chain amino acid ABC t... 231 3e-62 gi|300312761|ref|YP_003776853.1| branched-chain amino acid ABC t... 145 2e-36 gi|300310948|ref|YP_003775040.1| branched-chain amino acid ABC t... 131 4e-32 gi|300313247|ref|YP_003777339.1| branched-chain amino acid ABC t... 78 4e-16 gi|300310991|ref|YP_003775083.1| branched-chain amino acid ABC t... 73 2e-14 >gi|300309516|ref|YP_003773608.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 378 Score = 231 bits (590), Expect = 3e-62, Method: Compositional matrix adjust. Identities = 111/209 (53%), Positives = 154/209 (73%) Query: 1 MSLVRIIATALSLLSACAFAQERQVVKLGFSSPLSGPQAAAGKDALNGVQMGIERLNQQN 60 +S + IIA +L+L+ A A AQE QVVK+GFSSPL+GPQA+AGKD G+ M IERLN Q Sbjct: 5 VSGLNIIAASLALIPAFAMAQETQVVKIGFSSPLTGPQASAGKDNQGGLMMAIERLNAQP 64 Query: 61 LQLDGKAIRFELLVRDDKASAQEGAAVARELVAAGVRAVLGPFNSGVALSAAKIYNDAGV 120 + + GK I+F+++ DD+A + G AVA++L GV+A++GP+NSGV + A+++YNDAG+ Sbjct: 65 ITVGGKKIKFDVIAEDDQADPKSGVAVAQKLADQGVKAIVGPYNSGVTIPASRVYNDAGI 124 Query: 121 VALTVASNPKVTQTSLPQVFRIAASDTDMGGKMAFYAARHLKLKRVAIIEDGSPYARGLT 180 V TVASNPK+TQ +FR+AASD+ +GGKMA YAA+ LK KRVA+I+D + Y +GL Sbjct: 125 VVATVASNPKITQQGFATLFRVAASDSQLGGKMALYAAKELKFKRVAVIDDRTAYGQGLA 184 Query: 181 DEFERVAKMNGVQLVYKEAVGDKLEEFGA 209 EF +VAK NG+ +V + DK +F A Sbjct: 185 QEFIKVAKANGIDVVSTDFTNDKATDFTA 213 >gi|300312761|ref|YP_003776853.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 380 Score = 145 bits (367), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 77/197 (39%), Positives = 117/197 (59%), Gaps = 3/197 (1%) Query: 14 LSACAFAQERQVVKLGFSSPLSGPQAAAGKDALNGVQMGIERLNQQNLQLDGKAIRFELL 73 + A++QE VVK+ PLSGP A GKD NG +M ++ LN + + GK ++FEL+ Sbjct: 18 FAGAAYSQE--VVKIAHVGPLSGPNAHMGKDNENGARMAVDELNAKGFTIGGKKVKFELV 75 Query: 74 VRDDKASAQEGAAVARELVAAGVRAVLGPFNSGVALSAAKIYNDAGVVALT-VASNPKVT 132 DD + ++ AVA +LV V AV+G NSG + A+KIY+DAG+ ++ A+NPK T Sbjct: 76 GEDDASDPKQATAVATKLVDQKVAAVIGHLNSGTTIPASKIYSDAGIPQVSPSATNPKYT 135 Query: 133 QTSLPQVFRIAASDTDMGGKMAFYAARHLKLKRVAIIEDGSPYARGLTDEFERVAKMNGV 192 Q FR+ A+D +G + YA + L K++A+I+D + Y +G+ DEF + AK G Sbjct: 136 QQGFKTAFRVVANDAQLGAALGKYAVQKLGTKQIAVIDDRTAYGQGVADEFAKGAKAAGG 195 Query: 193 QLVYKEAVGDKLEEFGA 209 +V + DK +F A Sbjct: 196 TIVGTQYTNDKATDFNA 212 >gi|300310948|ref|YP_003775040.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 394 Score = 131 bits (330), Expect = 4e-32, Method: Compositional matrix adjust. Identities = 70/191 (36%), Positives = 113/191 (59%), Gaps = 1/191 (0%) Query: 21 QERQVVKLGFSSPLSGPQAAAGKDALNGVQMGIERLNQQNLQLDGKAIRFELLVRDDKAS 80 QE + +GF+ PLSGP GK NGVQ+ + N++ L++ GK+ + L+ +DD+A Sbjct: 35 QETATLAIGFAGPLSGPSGGVGKSMQNGVQLALAEANRRGLRIGGKSWQLRLIAQDDRAD 94 Query: 81 AQEGAAVARELVAAGVRAVLGPFNSGVALSAAKIYNDAGVVALTV-ASNPKVTQTSLPQV 139 VAR LV V AV+G + SG +L+AA +Y+ AGV+ +T A + ++T S P++ Sbjct: 95 PATAEFVARSLVQQQVIAVVGHWTSGTSLAAAPVYHRAGVIQVTPSAMSRRLTAMSYPRI 154 Query: 140 FRIAASDTDMGGKMAFYAARHLKLKRVAIIEDGSPYARGLTDEFERVAKMNGVQLVYKEA 199 FR ++ +G A YA L+ + + I+D +P+ +GL ++F R A G Q+V + + Sbjct: 155 FRTIPNNESLGRLAAGYAVDKLETRTIVTIDDRTPFGQGLAEQFARTASERGAQVVGRYS 214 Query: 200 VGDKLEEFGAA 210 V DK +F AA Sbjct: 215 VSDKTSDFNAA 225 >gi|300313247|ref|YP_003777339.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 385 Score = 78.2 bits (191), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 56/180 (31%), Positives = 91/180 (50%), Gaps = 7/180 (3%) Query: 5 RIIATALSLLS--ACAFAQERQVVKLGFSSPLSGPQAAAGKDALNGVQMGIERLNQQNLQ 62 R++A L L + +AQER+ + LGF+ LS P A + AL G Q+ I+ N + Sbjct: 8 RVLAGVLLLWAFMQAGWAQERKTIALGFTGRLSEPIA---QSALQGAQLAIDEANALAAK 64 Query: 63 LDGKAIRFELLVRDDKASAQEGAAVARELVAAGVRAVLGPFNSGVALSAAKIYNDAGVVA 122 +RFELL +DD + +AR V V V+GP++S AL+ A IY A + Sbjct: 65 RRSP-LRFELLPQDDHGNENFAVNIARYYVKEKVAGVIGPWSSDAALATAAIYESAQIPQ 123 Query: 123 L-TVASNPKVTQTSLPQVFRIAASDTDMGGKMAFYAARHLKLKRVAIIEDGSPYARGLTD 181 + A++ + T FR+ ++ +A A R L+ +R+ +I+ + Y+R L D Sbjct: 124 IGFTATSSQWTGMGNRMPFRVVGGTRELAATLAEVATRSLQGRRIFVIQSDAAYSRALAD 183 >gi|300310991|ref|YP_003775083.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 373 Score = 72.8 bits (177), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 47/158 (29%), Positives = 80/158 (50%), Gaps = 5/158 (3%) Query: 24 QVVKLGFSSPLSGPQAAAGKDALNGVQMGIERLNQQNLQLDGKAIRFELLVRDDKASAQE 83 + VK+ + P++G A G G IE++N +FE ++ DD ++ Sbjct: 27 ETVKIAIAGPMTGAVAQYGDMVKAGALTAIEQINAAG---GANGNKFEAVMMDDACEPKQ 83 Query: 84 GAAVARELVAAGVRAVLGPFNSGVALSAAKIYNDAGVVALT-VASNPKVTQTSLPQ-VFR 141 AVA ++V+ G++ V+G SG + A+ IY + GVV +T A+ P++T+ + +FR Sbjct: 84 AVAVANKIVSQGIKYVIGHVCSGSTIPASDIYENEGVVMITPSATAPQLTEAKPHKFIFR 143 Query: 142 IAASDTDMGGKMAFYAARHLKLKRVAIIEDGSPYARGL 179 D G A Y LK K+VA++ D Y +G+ Sbjct: 144 TIGRDDQQGPAAARYVIEKLKPKKVAVLHDKQSYGQGV 181 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.132 0.355 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 607,215 Number of extensions: 24244 Number of successful extensions: 172 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 168 Number of HSP's successfully gapped: 7 Length of query: 211 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 130 Effective length of database: 1,236,335 Effective search space: 160723550 Effective search space used: 160723550 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)