BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0148.0036.p (169 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309517|ref|YP_003773609.1| proline racemase [Herbaspirillu... 233 5e-63 >gi|300309517|ref|YP_003773609.1| proline racemase [Herbaspirillum seropedicae SmR1] Length = 336 Score = 233 bits (595), Expect = 5e-63, Method: Compositional matrix adjust. Identities = 108/123 (87%), Positives = 115/123 (93%) Query: 19 MLSYTRTVQTIDAHTGGEPLRILVSGLPQVPGATILEKRTWLKENRDDLRQFLMNEPRGH 78 MLSYTRTVQTIDAHTGGEPLRI+VSGLP++P TILE+R WL+E+RDDLR FLMNEPRGH Sbjct: 1 MLSYTRTVQTIDAHTGGEPLRIVVSGLPKIPAGTILEQRAWLREHRDDLRCFLMNEPRGH 60 Query: 79 ADMYGAYLFPPLTPNADFGVIFIHNEGYSDMCGHGIIALGKVLVEMGYVERTSPVTRICF 138 ADMYGAYL PP+T ADFGVIFIHNEGYSDMCGHGIIALGKVLVEMGYVERTSP TRICF Sbjct: 61 ADMYGAYLLPPVTAGADFGVIFIHNEGYSDMCGHGIIALGKVLVEMGYVERTSPQTRICF 120 Query: 139 DAP 141 DAP Sbjct: 121 DAP 123 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.141 0.454 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 590,708 Number of extensions: 24777 Number of successful extensions: 63 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 63 Number of HSP's successfully gapped: 1 Length of query: 169 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 90 Effective length of database: 1,245,805 Effective search space: 112122450 Effective search space used: 112122450 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 127 (53.5 bits)