BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0148.0030.p (405 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309520|ref|YP_003773612.1| branched-chain amino acid ABC t... 715 0.0 gi|300310522|ref|YP_003774614.1| branched-chain amino acid ABC t... 691 0.0 gi|300310993|ref|YP_003775085.1| branched-chain amino acid ABC t... 271 7e-74 gi|300311115|ref|YP_003775207.1| branched-chain amino acid ABC t... 127 2e-30 gi|300312238|ref|YP_003776330.1| amino acid ABC transporter perm... 124 1e-29 >gi|300309520|ref|YP_003773612.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 404 Score = 715 bits (1846), Expect = 0.0, Method: Compositional matrix adjust. Identities = 361/405 (89%), Positives = 382/405 (94%), Gaps = 1/405 (0%) Query: 1 MAFLSFDKKRNPRQANISLVALLILMIIFPFIASQFGNSWVRIMDIALLYIMLALGLNVV 60 MA L+FD KRNP+QA ISL+ LL LMI+FPF+A QFGNSWVRIMD+ALLYIMLALGLNVV Sbjct: 1 MALLTFDMKRNPQQARISLLLLLALMIVFPFVAQQFGNSWVRIMDVALLYIMLALGLNVV 60 Query: 61 VGFAGLLDLGYIAFYAIGAYTAGLLASPQFADVLQSFVNTYPAVGNFLVMVCGPQIVQTG 120 VGFAGLLDLGYIAFYAIGAY+AGLLASPQFA V++SFVNTYP+VGNFLV +CGP+IVQ G Sbjct: 61 VGFAGLLDLGYIAFYAIGAYSAGLLASPQFAAVIESFVNTYPSVGNFLVWLCGPEIVQNG 120 Query: 121 IHLSLWIIVPLSALIAALFGALLGAPTLKLRGDYLAIVTLGFGEIIRIFMNNLNAPVNIT 180 IHLSLW+IVP+SA +AALFGALLGAPTLKLRGDYLAIVTLGFGEIIRIFMNNLNAPVNIT Sbjct: 121 IHLSLWLIVPISAFLAALFGALLGAPTLKLRGDYLAIVTLGFGEIIRIFMNNLNAPVNIT 180 Query: 181 NGPQGINLIDPIRVFGVSLAGEPGSGSIVKFLGFSMPSVNAYYFLFLLLCIGVIFFSIRL 240 NGPQGINLIDPI+VFGVSLAGEPGSGS+VK G SMPSVNAYYFLFLLLCIGVIFFS+RL Sbjct: 181 NGPQGINLIDPIKVFGVSLAGEPGSGSMVKVFGMSMPSVNAYYFLFLLLCIGVIFFSVRL 240 Query: 241 QDSRLGRAWVAIREDEIAAKAMGINTRNVKLLAFAMGASFGGVAGAMFASFQGFVSPESF 300 QDSRLGRAWVAIREDEIAAKAMGINTRNVKLLAFAMGASFGGVAGAMF +FQGFVSPESF Sbjct: 241 QDSRLGRAWVAIREDEIAAKAMGINTRNVKLLAFAMGASFGGVAGAMFGAFQGFVSPESF 300 Query: 301 SLTESIAVLAMVVLGGIGHIPGVVLGGVILAALPEVLRHVVEPVQMSLFGKVWIDAEVLR 360 SLTESIAVLAMVVLGGIGHIPGVVLGGVILAALPEVLRHVVEPVQM++FGKVWIDAEVLR Sbjct: 301 SLTESIAVLAMVVLGGIGHIPGVVLGGVILAALPEVLRHVVEPVQMAIFGKVWIDAEVLR 360 Query: 361 QLLYGLAMVVIMLNRPAGLWPSPRHEDRPEADHPHGSDAVGVVKA 405 QLLYGLAMVVIML RPAGLWPSPRHEDRPEADH G VKA Sbjct: 361 QLLYGLAMVVIMLTRPAGLWPSPRHEDRPEADHTQ-VGTTGEVKA 404 >gi|300310522|ref|YP_003774614.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 404 Score = 691 bits (1784), Expect = 0.0, Method: Compositional matrix adjust. Identities = 362/405 (89%), Positives = 383/405 (94%), Gaps = 1/405 (0%) Query: 1 MAFLSFDKKRNPRQANISLVALLILMIIFPFIASQFGNSWVRIMDIALLYIMLALGLNVV 60 MA L+FD KRNP+QA ISL+ LL LMI+FPF+A QFGNSWVRIMD+ALLYIMLALGLNVV Sbjct: 1 MALLTFDMKRNPQQARISLLLLLALMIVFPFVAQQFGNSWVRIMDVALLYIMLALGLNVV 60 Query: 61 VGFAGLLDLGYIAFYAIGAYTAGLLASPQFADVLQSFVNTYPAVGNFLVMVCGPQIVQTG 120 VGFAGLLDLGYIAFYAIGAY+AGLLASPQFA V++SFVNTYP+VGNFLV +CGP+IVQ G Sbjct: 61 VGFAGLLDLGYIAFYAIGAYSAGLLASPQFAAVIESFVNTYPSVGNFLVWLCGPEIVQNG 120 Query: 121 IHLSLWIIVPLSALIAALFGALLGAPTLKLRGDYLAIVTLGFGEIIRIFMNNLNAPVNIT 180 IHLSLW+IVP+SAL+AALFGALLGAPTLKLRGDYLAIVTLGFGEIIRIFMNNLNAPVNIT Sbjct: 121 IHLSLWLIVPISALLAALFGALLGAPTLKLRGDYLAIVTLGFGEIIRIFMNNLNAPVNIT 180 Query: 181 NGPQGINLIDPIRVFGVSLAGEPGSGSIVKFLGFSMPSVNAYYFLFLLLCIGVIFFSIRL 240 NGPQGINLIDPI+VFGVSLAGEPGSGS+VK G SMPSVNAYYFLFLLLCIGVIFFS+RL Sbjct: 181 NGPQGINLIDPIKVFGVSLAGEPGSGSMVKVFGMSMPSVNAYYFLFLLLCIGVIFFSVRL 240 Query: 241 QDSRLGRAWVAIREDEIAAKAMGINTRNVKLLAFAMGASFGGVAGAMFASFQGFVSPESF 300 QDSRLGRAWVAIREDEIAAKAMGINTRNVKLLAFAMGASFGGVAGAMF +FQGFVSPESF Sbjct: 241 QDSRLGRAWVAIREDEIAAKAMGINTRNVKLLAFAMGASFGGVAGAMFGAFQGFVSPESF 300 Query: 301 SLTESIAVLAMVVLGGIGHIPGVVLGGVILAALPEVLRHVVEPVQMSLFGKVWIDAEVLR 360 SLTESIAVLAMVVLGGIGHIPGVVLGGVILAALPEVLRHVVEPVQM++FGKVWIDAEVLR Sbjct: 301 SLTESIAVLAMVVLGGIGHIPGVVLGGVILAALPEVLRHVVEPVQMAIFGKVWIDAEVLR 360 Query: 361 QLLYGLAMVVIMLNRPAGLWPSPRHEDRPEADHPHGSDAVGVVKA 405 QLLYGLAMVVIML RPAGLWPSPRHEDRPEADH G VKA Sbjct: 361 QLLYGLAMVVIMLTRPAGLWPSPRHEDRPEADHTQ-VGTTGEVKA 404 >gi|300310993|ref|YP_003775085.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 408 Score = 271 bits (693), Expect = 7e-74, Method: Compositional matrix adjust. Identities = 166/389 (42%), Positives = 228/389 (58%), Gaps = 61/389 (15%) Query: 9 KRNPRQANISLVALLILMIIFPFIASQFGNSWVRIMDIALLYIMLALGLNVVVGFAGLLD 68 + PRQ +++ LL+ +++PF S+ +V +M +AL+Y++L LGLN+VVGFAGLLD Sbjct: 74 RMQPRQQRAAVMILLMAALVWPFFGSR---GYVDVMTLALIYVVLGLGLNIVVGFAGLLD 130 Query: 69 LGYIAFYAIGAYTAGLLASPQFADVLQSFVNTYPAVGNFLVMVCGPQIVQTGIHLSLWII 128 LGY+ FYA+GAYT L N Y L+ W Sbjct: 131 LGYVGFYAVGAYTYAL-------------CNQY-------------------FGLTFWEC 158 Query: 129 VPLSALIAALFGALLGAPTLKLRGDYLAIVTLGFGEIIRIFMNNLNAPVNITNGPQGINL 188 VPL+A +ALFG LLG P L+LRGDYLAIVTLGFGEIIR+ +NN+ +IT GP G++ Sbjct: 159 VPLAAAASALFGFLLGFPVLRLRGDYLAIVTLGFGEIIRLLLNNMT---SITGGPDGVSG 215 Query: 189 IDPIRVFGVSLAGEP---GSGSIVKFLGFSMPS---VNAYYFLFLLLCIGVIFFSIRLQD 242 I VFG+ +A P G + + G S V YFL LL+ + F + RL Sbjct: 216 IPKPTVFGLVMARNPVTEGGTTFHQLFGLSYQGGHMVIFLYFLALLMVLFTYFVTSRLLR 275 Query: 243 SRLGRAWVAIREDEIAAKAMGINTRNVKLLAFAMGASFGGVAGAMFASFQGFVSPESFSL 302 +GRAW A+REDEIA +++GIN VKL AF +GA+F G+AG+ FA+ QG V+PESF+ Sbjct: 276 MPMGRAWEALREDEIACRSLGINPTKVKLSAFTLGAAFAGLAGSFFAARQGLVTPESFTF 335 Query: 303 TESIAVLAMVVLGGIGHIPGVVLGGVILAALPEVLRHVVEPVQMSLFGKVWIDAEVLRQL 362 ES +LA+VVLGG+G GV+L ++L LPE+ R E R L Sbjct: 336 IESALILAIVVLGGMGSQLGVILAAILLTVLPELARSFAE----------------YRML 379 Query: 363 LYGLAMVVIMLNRPAGLWPSPR-HEDRPE 390 ++GL MV++M+ RP GL P+ R H + P+ Sbjct: 380 IFGLVMVLMMMWRPQGLLPATRPHVELPQ 408 >gi|300311115|ref|YP_003775207.1| branched-chain amino acid ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 655 Score = 127 bits (319), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 85/301 (28%), Positives = 154/301 (51%), Gaps = 54/301 (17%) Query: 40 WVRIMDIALLYIMLALGLNVVVGFAGLLDLGYIAFYAIGAYTAGLLASPQFADVLQSFVN 99 ++ +++ L+Y +L GL++VVG+ G + LG+ + IG+Y AG+L Sbjct: 28 YLHLIETILIYAILLFGLDIVVGYTGQVSLGHAGLFGIGSYAAGVL-------------- 73 Query: 100 TYPAVGNFLVMVCGPQIVQTGIHLSLWIIVPLSALIAALFGALLGAPTLKLRGDYLAIVT 159 + +G + +++ VP + + A+FGA+L P L++ G YLA+VT Sbjct: 74 -FFKLG-----------------MPIFVTVPAAIAVTAVFGAILALPALRVTGPYLAMVT 115 Query: 160 LGFGEIIRIFMNNLNAPVNITNGPQGINLIDPIRVFGVSLAGEPGSGSIVKFLGFSMPSV 219 L FG II+I +N + +T GP GI L P+ G M V Sbjct: 116 LAFGTIIQILINEMTF---LTEGPLGIKLQKPM------------------IGGDKMSEV 154 Query: 220 NAYYFLFLLLCIGVIFFSIRLQDSRLGRAWVAIREDEIAAKAMGINTRNVKLLAFAMGAS 279 +Y + +L+ + ++ R+ S LGRA+ A+R+ IA+ MG++ K+ AF + A+ Sbjct: 155 QFFYLVAVLMVLSMLVVH-RILKSNLGRAFQALRDSPIASDCMGVSVYRYKVYAFIISAA 213 Query: 280 FGGVAGAMFASFQGFVSPESFSLTESIAVLAMVVLGGIGHIPGVVLGGVILAALPEVLRH 339 G++GA++A + ++SP +++ +I L V++GG G ++G +I+ LP +L Sbjct: 214 LAGLSGALYAYSEEYISPNTYNFELTIMFLLAVIMGGRKSRIGSLIGALIVVMLPSLLSD 273 Query: 340 V 340 + Sbjct: 274 I 274 >gi|300312238|ref|YP_003776330.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 288 Score = 124 bits (311), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 100/288 (34%), Positives = 144/288 (50%), Gaps = 65/288 (22%) Query: 52 MLALGLNVVVGFAGLLDLGYIAFYAIGAYTAGLLASPQFADVLQSFVNTYPAVGNFLVMV 111 MLAL + V + GLL L AF IGAYTA LL F +P Sbjct: 23 MLALSIYVTLS-CGLLSLANAAFMGIGAYTASLLT--------MQFEMPFP--------- 64 Query: 112 CGPQIVQTGIHLSLWIIVPLSALIAALFGALLGAPTLKLRGDYLAIVTLGFGEIIRIFMN 171 I + ++ AL ++G PTL+L G YLA+ TLGFGE++R+ + Sbjct: 65 ---------------IALAAGGVLPALVALVIGIPTLRLSGVYLAMATLGFGEVVRVIVL 109 Query: 172 NLNAPVNITNGPQGINLIDPIRVFGVSLAGEPGSGSIVKFLGFSMPSVNAYYFLFLLLCI 231 NL+ IT GP G+N +P + ++ + LLL + Sbjct: 110 NLD----ITGGPMGLN---------------------------GVPPITEWWHIVLLLGV 138 Query: 232 GVIFFSIRLQDSRLGRAWVAIREDEIAAKAMGINTRNVKLLAFAMGASFGGVAGAMFASF 291 I+ RL+ S++GRA+ AI+EDEIAA+ MG+N KLLAF +GA+ GVAG + A F Sbjct: 139 -TIYVLARLRRSKIGRAFEAIKEDEIAARLMGVNVAGYKLLAFVIGAAIAGVAGGLNAHF 197 Query: 292 QGFVSPESFSLTESIAVLAMVVLGGIGHIPGVVLGGVILAALPEVLRH 339 + P ++ ++ +L M V GG ++ G ++G IL+ LPEVLRH Sbjct: 198 TFTIGPNNYGFENAVDILTMAVFGGTSNLIGPMIGSTILSLLPEVLRH 245 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.144 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,252,429 Number of extensions: 52583 Number of successful extensions: 347 Number of sequences better than 1.0e-08: 25 Number of HSP's gapped: 324 Number of HSP's successfully gapped: 26 Length of query: 405 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 318 Effective length of database: 1,207,925 Effective search space: 384120150 Effective search space used: 384120150 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)