BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0148.0021.p (98 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310527|ref|YP_003774619.1| transmembrane protein [Herbaspi... 131 9e-33 gi|300313674|ref|YP_003777766.1| hypothetical protein Hsero_4391... 86 8e-19 >gi|300310527|ref|YP_003774619.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 144 Score = 131 bits (330), Expect = 9e-33, Method: Compositional matrix adjust. Identities = 62/98 (63%), Positives = 79/98 (80%) Query: 1 LRTVRSDVQVLLQEAQELFTEAAATTGKKAEDLRAKGEEILDRAFIKAQGIQSYVVQTGK 60 +TVR+DVQ LLQ+AQ++F EAA TGKKA+DLRA +E+L++A AQ Q+ VV TG+ Sbjct: 46 FKTVRTDVQSLLQQAQDMFAEAANATGKKADDLRASAQELLEQALNTAQDAQAAVVDTGR 105 Query: 61 EIVSTTDDYVQSNPWRAIAISTGLGLLLGLCVSRTGER 98 +IV+TTDDYVQSNPWRAIAI TG+GLL+GL V R +R Sbjct: 106 QIVTTTDDYVQSNPWRAIAIGTGIGLLVGLAVGRCADR 143 >gi|300313674|ref|YP_003777766.1| hypothetical protein Hsero_4391 [Herbaspirillum seropedicae SmR1] Length = 102 Score = 85.5 bits (210), Expect = 8e-19, Method: Compositional matrix adjust. Identities = 51/95 (53%), Positives = 73/95 (76%) Query: 1 LRTVRSDVQVLLQEAQELFTEAAATTGKKAEDLRAKGEEILDRAFIKAQGIQSYVVQTGK 60 L+TVR+D++ L+++AQ LF EAAA TG+KA++LRA+G +L+ A AQ Q+ ++ GK Sbjct: 7 LKTVRNDMKTLVKDAQALFAEAAAATGEKADELRARGLTLLEAAAEAAQSAQAAAIEKGK 66 Query: 61 EIVSTTDDYVQSNPWRAIAISTGLGLLLGLCVSRT 95 E+ + TDDYV NPWRA+AIS GLGLL+GL + R+ Sbjct: 67 EVAAKTDDYVHENPWRAVAISAGLGLLVGLVIGRS 101 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.131 0.355 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 225,863 Number of extensions: 6799 Number of successful extensions: 37 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 37 Number of HSP's successfully gapped: 2 Length of query: 98 Length of database: 1,619,870 Length adjustment: 67 Effective length of query: 31 Effective length of database: 1,302,625 Effective search space: 40381375 Effective search space used: 40381375 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 123 (52.0 bits)