BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0148.0013.p (359 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310534|ref|YP_003774626.1| transmembrane sensor histidine ... 160 1e-40 >gi|300310534|ref|YP_003774626.1| transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 677 Score = 160 bits (406), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 131/299 (43%), Positives = 189/299 (63%), Gaps = 4/299 (1%) Query: 7 DIGESSSLEHYVATQANFASIISAAMSLAMIIALFGVVVLSSCYAEDGYRKILADLIAVQ 66 DI S+S+E++ A+ A+++S+ + AM LAM + L G+VVL + + YR++L+ L+ ++ Sbjct: 14 DIQNSASVEYFAASHAHYSSMATLAMGLAMAVILLGLVVLLASLLGEEYRRLLSGLLEMR 73 Query: 67 GSSAAGFILSGVALYLQCLNVDKTSRKLALAARVLATLLLLLSAGFLLQNLFGVKLLHAA 126 +A G IL ALYL+C++ +R+ L RV A L +L A L G+ Sbjct: 74 EMTAVGLILGASALYLRCIH--GGARRSGLR-RVAAGLSWMLLALGLGTLAHGLMYALGW 130 Query: 127 LPVLSMAGSAFGAEIHPLAAGCFLLTALILLLQELRIRGGHYPAEYLAFILIGLSAIPLV 186 LP+ I PL + F++ A +LLLQ+ R+ GG+YPAEYL+F LIGL+AIPLV Sbjct: 131 LPI-DQNHPLSPLVIPPLLSLGFVVMAAVLLLQDWRMLGGYYPAEYLSFALIGLAAIPLV 189 Query: 187 GYLYGISKLTFLRYSTGIPLLTALGFLVLGAALLMARPSHRLMSIIIGHAPGGNMLRRLL 246 GYLYG+S+ +L + + LL+AL +L ALLMARP+H LM+II APGG MLR LL Sbjct: 190 GYLYGVSQFVYLDFPLPVSLLSALVMALLATALLMARPAHPLMAIITRIAPGGQMLRHLL 249 Query: 247 PSMLFLILLLNLLVAKGAERGLYSDQLMLPFLTLIDGALVVFVFWRFAGKLDREYGARA 305 P +FL+L +LL+ G G +L+LP LTLI+G +V+F+FW A +LD EYG R Sbjct: 250 PQTVFLLLAFSLLLNWGMTHGWILPELLLPTLTLINGVIVLFIFWGSASRLDSEYGERT 308 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.142 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 859,683 Number of extensions: 28425 Number of successful extensions: 119 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 118 Number of HSP's successfully gapped: 1 Length of query: 359 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 273 Effective length of database: 1,212,660 Effective search space: 331056180 Effective search space used: 331056180 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 131 (55.1 bits)