BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0148.0012.p (290 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310534|ref|YP_003774626.1| transmembrane sensor histidine ... 476 e-136 gi|300311886|ref|YP_003775978.1| multi-sensor signal transductio... 123 2e-29 gi|300311282|ref|YP_003775374.1| transmembrane sensor histidine ... 85 6e-18 gi|300312939|ref|YP_003777031.1| transmembrane nitrate/nitrite s... 74 2e-14 gi|300312076|ref|YP_003776168.1| signal transduction histidine k... 67 2e-12 >gi|300310534|ref|YP_003774626.1| transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 677 Score = 476 bits (1225), Expect = e-136, Method: Compositional matrix adjust. Identities = 228/286 (79%), Positives = 259/286 (90%) Query: 1 MAHGLPETIEQTLHLQHGVFTYQSTRVPWFDKAGNVLGVIGISTDISERKRAEDSLRERE 60 MA G PE +EQTLHL GV T+Q+ +VPWF K G+V GVIGISTDI+ERK+AED LR+RE Sbjct: 385 MASGKPEKLEQTLHLPEGVVTFQTAKVPWFGKDGSVQGVIGISTDITERKQAEDELRQRE 444 Query: 61 AQLEKTVVERTALLRELTNHLETVREEEKRAIARELHDNMGASLTALSMHLESVYKILPE 120 +QLEKTV++RTALLRELTNHLETVREEEKRAIARELHDNMGASLTALSMHLE VY+ILP Sbjct: 445 SQLEKTVIQRTALLRELTNHLETVREEEKRAIARELHDNMGASLTALSMHLEGVYQILPP 504 Query: 121 DEKWQERKPRMQGLMSSLVSTTRRIQTELRPNMLDLFGLKAAITEQLDDLAERTGMTCKA 180 DEKW +RK RMQGLM SLV+TTRRIQTELRPN LDLFGLKAAI+EQLD+L ERTG+ C A Sbjct: 505 DEKWADRKVRMQGLMKSLVATTRRIQTELRPNTLDLFGLKAAISEQLDELHERTGIACHA 564 Query: 181 SLPDEDVTIGHEMEIAIYRMLQEMLNNVTKHAKANQVDVILDVDEEQVSLTVRDNGVGIP 240 SLPDEDV +GHEMEIAIYRMLQEMLNNVTKHAKA++VDVILD+DE+ V+LTVRD+GVGIP Sbjct: 565 SLPDEDVEVGHEMEIAIYRMLQEMLNNVTKHAKASKVDVILDIDEDHVALTVRDDGVGIP 624 Query: 241 QERQDNNKTYGLRGLRERATFFGGNVVIRSTPGNGSIISIRLPLAP 286 +ER+DN+KTYGLRGLRERATFFGG V IRSTPG G++I+I LP+ P Sbjct: 625 EERRDNHKTYGLRGLRERATFFGGEVDIRSTPGKGALITISLPIRP 670 >gi|300311886|ref|YP_003775978.1| multi-sensor signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 123 bits (308), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 92/291 (31%), Positives = 158/291 (54%), Gaps = 36/291 (12%) Query: 13 LHLQHGVFTYQSTR----VPWFDKAGNVLG--VIGISTDISERKRAEDSLREREAQLEKT 66 LH Q VF + V F +G G V G++ D++ER+++ L + Sbjct: 201 LHTQETVFDHNHNARILDVVRFPISGEGKGDMVAGLAIDVTEREQSRRDL--------EL 252 Query: 67 VVERTALLRELTNHLETVREEEKRAIARELHDNMGASLTALSMHLESVYKILPEDEKWQE 126 ++R LL ++ + V+E E++ +AR LHD++G SLTAL ++LE++ K+ + Sbjct: 253 AIQRQQLL---SSRVIEVQERERQHLARGLHDDVGQSLTALKINLETIKKMGSLE----- 304 Query: 127 RKPRMQG---LMSSLVSTTRRIQTELRPNMLDLFGLKAAI---TEQLDDLAERTGMTCKA 180 P +Q +++++++ R + +LRP LD GL AA+ EQ LA G ++ Sbjct: 305 -GPSLQNGIDIVTAVLAQVRSLSLDLRPPQLDNLGLIAALRSYVEQKSKLANVNGW-FES 362 Query: 181 SLPDEDVTIGHEMEIAIYRMLQEMLNNVTKHAKANQVDVILDVDEEQVSLTVRDNGVGIP 240 S DE + H++E +R++QE + N+ +HA+ + V +D +QV L++RD+G G Sbjct: 363 SGHDE--QLHHDIENTAFRIVQEAVTNILRHARCQNIWVKIDRQHDQVYLSIRDDGRGFD 420 Query: 241 QERQDNNK----TYGLRGLRERATFFGGNVVIRSTPGNGSIISIRLPLAPV 287 ER +N ++GL + ERA GG++ I S PG G+ I + LP+AP+ Sbjct: 421 IERARSNAISGTSFGLLNMEERAVLVGGSMEITSAPGAGTEIVMHLPVAPI 471 >gi|300311282|ref|YP_003775374.1| transmembrane sensor histidine kinase transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 464 Score = 85.1 bits (209), Expect = 6e-18, Method: Compositional matrix adjust. Identities = 62/224 (27%), Positives = 112/224 (50%), Gaps = 19/224 (8%) Query: 72 ALLRELTNHLETVREEEKRAIARELHDNMGASLTALSMHLESVYKIL---PEDEK----- 123 A LR+L + +EEE+ ++R+LHD + L ++ + +E+ L PE Sbjct: 233 AKLRQLAQRVVRSQEEERARLSRDLHDGISQWLVSIKLQIEAALVRLNGTPEQAATARAG 292 Query: 124 WQERKPRMQGLMSSLVSTTRRIQTELRPNMLDLFGLKAAITEQLDDLAERTGMTCKASL- 182 +++ ++ G+++ + RRI +LRP +LD GL AA+ + + G + Sbjct: 293 FEKTATQINGVLAEV----RRISHDLRPAILDDLGLAAALEHLCGEWRDAAGAGVEIVFE 348 Query: 183 ----PDEDVTIGHEMEIAIYRMLQEMLNNVTKHAKANQVDVILDVDEEQVSLTVRDNGVG 238 D+ T+ + ++R+ QE L N+ +HA A+++ V L + L V+DNG G Sbjct: 349 NQAQADDGATLPDVVNTVLFRIAQEALTNIARHAGASRIKVRLYQGHNLLMLQVQDNGHG 408 Query: 239 IPQERQDNNKTY--GLRGLRERATFFGGNVVIRSTPGNGSIISI 280 + D++ + GLR +RER GG + I S+P SI ++ Sbjct: 409 FDLGQVDDHSRHGIGLRNMRERIEAVGGELGIDSSPAGTSISAV 452 >gi|300312939|ref|YP_003777031.1| transmembrane nitrate/nitrite sensor histidine kinase transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 650 Score = 73.6 bits (179), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 54/199 (27%), Positives = 104/199 (52%), Gaps = 6/199 (3%) Query: 88 EKRAIARELHDNMGASLTALSMHLESVYKILPED--EKWQERKPRMQGLMSSLVSTTRRI 145 E+ +A+ LHD++ L L++ ++ + L D ++ ++ P ++ + R + Sbjct: 446 ERNLLAQGLHDSIAQGLNFLNLQVQMLEDSLARDRLDEIRDIAPLLRTGVQENYEDVREL 505 Query: 146 QTELRPNMLDLFGLKAAITEQLDDLAERTGMTCKASLPDEDVTIGHEMEIAIYRMLQEML 205 R + D L + + + LD L ++G+ + +L + + E ++ + +LQE L Sbjct: 506 LLNFRTRLQD-SNLDSEMRKALDKLQRQSGLQGQITLTGDGPPLAPEQQLQVLFILQEAL 564 Query: 206 NNVTKHAKANQVDVILDVDEEQVSLTVRDNGVGIPQE--RQDNNKTYGLRGLRERATFFG 263 +N+ KHA+A QV + +D + + +TV D+G G E R + GLR ++ERA G Sbjct: 565 SNIRKHARAQQVQISVD-NRQDFRMTVSDDGHGFTLESARAKGLEHVGLRIMQERAERLG 623 Query: 264 GNVVIRSTPGNGSIISIRL 282 N+ I S PG+G+ I++ L Sbjct: 624 ANLEIDSQPGHGTRIALTL 642 >gi|300312076|ref|YP_003776168.1| signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 356 Score = 67.0 bits (162), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 56/212 (26%), Positives = 104/212 (49%), Gaps = 16/212 (7%) Query: 84 VREEEKRAIARELHDNMGASLTALSMHLESVYKIL-------PEDEKWQERKPRMQGLMS 136 +RE+E+ IAR+LHD++G L + M L + + L P E + R+ L+ Sbjct: 150 IREQERERIARDLHDDLGGVLAGVGMALGRLQEQLAAQGLAAPLAEADEARQ-----LLQ 204 Query: 137 SLVSTTRRIQTELRPNMLDLFGLKAAITEQLDDLAERTGMTCKASLPDEDVTIGHEMEIA 196 +++ I EL P +++ FGL A+ Q + + C+ P + + Sbjct: 205 QAMASMHSIIDELHPPIVE-FGLVDALQWQCRQIQREYRLHCQLDCPPTLEQEDRFLILG 263 Query: 197 IYRMLQEMLNNVTKHAKANQVDVILDVDEEQVSLTVRDNGVGI-PQERQD--NNKTYGLR 253 + R+L+E +N +H +A+ + V L + + ++ + + D+G GI P ++ +GLR Sbjct: 264 LLRILREAASNAARHGRASALRVDLRMRQARLEMQIADDGCGIAPLHAKEVAPRHGHGLR 323 Query: 254 GLRERATFFGGNVVIRSTPGNGSIISIRLPLA 285 +RERA GG + I + G +I +P+A Sbjct: 324 NMRERAQALGGELFIEAGQDGGVVIRTLIPVA 355 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.132 0.367 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 847,552 Number of extensions: 32833 Number of successful extensions: 193 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 189 Number of HSP's successfully gapped: 5 Length of query: 290 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 206 Effective length of database: 1,222,130 Effective search space: 251758780 Effective search space used: 251758780 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)