BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0148.0001.p
         (138 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310292|ref|YP_003774384.1| 3-hydroxyisobutyrate dehydrogen...   132   1e-32

>gi|300310292|ref|YP_003774384.1| 3-hydroxyisobutyrate dehydrogenase
           [Herbaspirillum seropedicae SmR1]
          Length = 288

 Score =  132 bits (332), Expect = 1e-32,   Method: Compositional matrix adjust.
 Identities = 63/94 (67%), Positives = 73/94 (77%), Gaps = 2/94 (2%)

Query: 45  LDAQQIFEGINAGSGRSGVTQVNYPTWIFNDAFDSGFTMKLMRKDLRLAMGLLGELGTSA 104
           LDA+QIF+G+ AGS  S V +V YPTWIFN+AFDSGFTMKLMRKDLRLAM L+ E  ++ 
Sbjct: 197 LDARQIFQGLAAGSANSKVIEVFYPTWIFNEAFDSGFTMKLMRKDLRLAMNLIEETRSTT 256

Query: 105 PLSGEVGRLWAASAEAAGTADGEDFNRIVQFIEK 138
           PL+ E GRLWA S E  G AD EDFNRIV +IE 
Sbjct: 257 PLAREAGRLWARSVE--GLADAEDFNRIVNYIEH 288



 Score = 54.3 bits (129), Expect = 3e-09,   Method: Compositional matrix adjust.
 Identities = 28/47 (59%), Positives = 30/47 (63%)

Query: 1  MKHIGVAGLGNMGRGMALSLQRGGFTVSGYDPATAAARIAAAAGLDA 47
          MK IGV GLGNMGRGMA SLQRGG+ V GYD   +     A  GL  
Sbjct: 1  MKKIGVIGLGNMGRGMAFSLQRGGYEVLGYDAQESTRTALAQQGLHC 47


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.319    0.135    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 375,074
Number of extensions: 12694
Number of successful extensions: 67
Number of sequences better than 1.0e-08: 1
Number of HSP's gapped: 66
Number of HSP's successfully gapped: 2
Length of query: 138
Length of database: 1,619,870
Length adjustment: 76
Effective length of query: 62
Effective length of database: 1,260,010
Effective search space: 78120620
Effective search space used: 78120620
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 126 (53.1 bits)