BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0147.0017.p (662 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309804|ref|YP_003773896.1| hydroxydechloroatrazine ethylam... 691 0.0 gi|300309803|ref|YP_003773895.1| nitrate/sulfonate/bicarbonate A... 291 1e-79 gi|300312557|ref|YP_003776649.1| hydroxy-atrazine ethyl amino hy... 216 6e-57 gi|300313548|ref|YP_003777640.1| nitrate/sulfonate/bicarbonate A... 85 1e-17 gi|300309985|ref|YP_003774077.1| nitrate/sulfonate/bicarbonate A... 57 7e-09 >gi|300309804|ref|YP_003773896.1| hydroxydechloroatrazine ethylaminohydrolase [Herbaspirillum seropedicae SmR1] Length = 471 Score = 691 bits (1782), Expect = 0.0, Method: Compositional matrix adjust. Identities = 342/470 (72%), Positives = 379/470 (80%), Gaps = 43/470 (9%) Query: 187 TSFLISNIDAIMTGLHGAAARHAGPDIRVVDGVIGAIGKLAPLPGEAVVDATDCIAYPSW 246 ++FLI N+ AIMTGL GAAARHAGPDIRV DGVI AIG+L LPGE ++DA+ C+AYP+W Sbjct: 2 STFLIRNLQAIMTGLPGAAARHAGPDIRVSDGVIAAIGQLDALPGEELIDASHCVAYPAW 61 Query: 247 VNTHHHLFQSMLKGDPAGIDASLTPWLAATPYRYRARFDEKLFRLAVRIGLVELMRSGCG 306 VNTHHHLFQS+LKGDP GI+A+LTPWLAATPYRYRA FD LFRLA RIG+VELMRSGCG Sbjct: 62 VNTHHHLFQSLLKGDPLGINATLTPWLAATPYRYRASFDADLFRLAARIGMVELMRSGCG 121 Query: 307 TVADHNYLYYPGMPYDTSAILFEEAEALGLRFVLCRGGATQTRQLEAELPTALRPETLDG 366 T+ADHNYLYYPGMP+DTS ILFEEA+ LGLRFVLCRG AT+TRQLEAEL +ALRPETLDG Sbjct: 122 TIADHNYLYYPGMPFDTSEILFEEAQQLGLRFVLCRGTATRTRQLEAELSSALRPETLDG 181 Query: 367 YLADMERLIKLYHDPSPMAMRRVVMAPTTPLGX--------------------------- 399 +LADM+ L +HDP+P +MRRVVMAPTTPL Sbjct: 182 FLADMQGLAGRFHDPAPASMRRVVMAPTTPLYSAAPDELREMAVAAREMGLRLHSHLSET 241 Query: 400 ----------------EFAERCDWLGDDVWFAHLVKLDPSEIALLGKTGTGIAHCPQSNG 443 EF ER WLG DVWFAHLVKLD EI LLG TGTGIAHCPQSNG Sbjct: 242 VGYQDSAHAMHGCRPVEFCERVGWLGRDVWFAHLVKLDAEEIQLLGGTGTGIAHCPQSNG 301 Query: 444 RLGSGIAPIRQLEEAGVPISIGVDGAASNEAADMVSETHAAWLMQRARRGQEATPSYAGG 503 RLGSGIAPIR LE AGVP+SIGVDGAASNEAADM+SETHAAWLMQRARRGQEATPS+ GG Sbjct: 302 RLGSGIAPIRALEAAGVPVSIGVDGAASNEAADMISETHAAWLMQRARRGQEATPSFRGG 361 Query: 504 SFEGGADAATVEDVVRWGTTGGARVLGLGALDGLQVGQQADIALYRLDDPRYFGLHDPAL 563 FEGGADAATVEDVVRWGTTGGARVLGL ALDGLQVGQQADIALYRLDDPRYFGLHDPA+ Sbjct: 362 HFEGGADAATVEDVVRWGTTGGARVLGLDALDGLQVGQQADIALYRLDDPRYFGLHDPAI 421 Query: 564 GPVVGGGRPYLKALYVAGRAVVEDDGIPGIDLAALGREARQATRRLLAAQ 613 GPV GGRP+LKA++V GRAVV +D IPG+DLA LG +ARQA +++L + Sbjct: 422 GPVASGGRPFLKAMWVGGRAVVREDAIPGVDLAELGAQARQAVKKMLKSN 471 >gi|300309803|ref|YP_003773895.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 267 Score = 291 bits (745), Expect = 1e-79, Method: Compositional matrix adjust. Identities = 145/180 (80%), Positives = 161/180 (89%) Query: 3 PLITNPKFLRIAAPFMIGVMLLLAWQLICTALAVPVYLVPTPSVIARTLAADWGLLSESL 62 PL+ P F R+AAP +IG+ LLL WQ++CT L VP YLVPTP++I +TLAADW +L SL Sbjct: 7 PLVNRPGFWRVAAPLIIGIALLLMWQIVCTLLEVPPYLVPTPALIVKTLAADWSMLMASL 66 Query: 63 LVTLKITFMAFVLSVLLGVAIAFVFVQSRFIEVSLFPYAIILQVTPIVAIAPLIIIWVKE 122 LVTLKITF+AF L+V+LGVAIAFVFVQSRFIE+SLFPYAIILQVTPIVAIAPLIIIWVK+ Sbjct: 67 LVTLKITFLAFALAVVLGVAIAFVFVQSRFIEISLFPYAIILQVTPIVAIAPLIIIWVKD 126 Query: 123 PTFALVICATIMALFPIISNTTLGLRSVNPGLLNLFRLNKATRWQILRRLRIPSALPYFF 182 T AL +CATIMALFPIISNTTLGLRSVNPGLLNLFRLNKATRWQ LRRLRIPSALPYFF Sbjct: 127 TTAALTVCATIMALFPIISNTTLGLRSVNPGLLNLFRLNKATRWQTLRRLRIPSALPYFF 186 >gi|300312557|ref|YP_003776649.1| hydroxy-atrazine ethyl amino hydrolase [Herbaspirillum seropedicae SmR1] Length = 458 Score = 216 bits (549), Expect = 6e-57, Method: Compositional matrix adjust. Identities = 150/460 (32%), Positives = 220/460 (47%), Gaps = 71/460 (15%) Query: 186 GTSFLISNIDAIMTGLHGAAARHAGPD--IRVVDGVIGAIGKLAPLPGEA--VVDATDCI 241 G + LI N ++T +R PD + + + VI +G+ A LP +A ++DA I Sbjct: 7 GKTLLIRNARILVTM---NESREEIPDGALYIRNNVIEQVGQTADLPQDADEIIDARHHI 63 Query: 242 AYPSWVNTHHHLFQSMLKGDPAGIDASLTPWLAATPYRYRARFDEKLFRLAVRIGLVELM 301 P +NTHHH++QS+ + P+ + L WL Y A ++ +++ + EL+ Sbjct: 64 VLPGLINTHHHMYQSLTRAIPSAQNGELFNWLTKL-YPIWAGLTPEMIQVSTLTAMAELI 122 Query: 302 RSGCGTVADHNYLYYPGMPYDTSAILFEEAEALGLRFVLCRGGATQTRQLEAELPTALRP 361 SGC T +DH Y+Y D S E A +G+RF R GA Q + LP Sbjct: 123 LSGCTTSSDHLYIYPNQTRLDDS---LEAAARIGMRFHGAR-GAMSVGQSKGGLPPDRVV 178 Query: 362 ETLDGYLADMERLIKLYHDPSPMAMRRVVMAPTTPLG----------------------- 398 + L D +RLI+ YHD AM+R+V+AP +P Sbjct: 179 QDEAAILKDTQRLIETYHDHQRHAMQRIVVAPCSPFSVSRELMREAADLARHYGVSLHTH 238 Query: 399 -------------------XEFAERCDWLGDDVWFAHLVKLDPSEIALLGKTGTGIAHCP 439 E+AE C W+G DVW AH V+LD I L +TGTG+AHCP Sbjct: 239 LAENVNDIAYSREKFNMSPAEYAEDCGWVGHDVWHAHCVQLDAHGIDLFARTGTGVAHCP 298 Query: 440 QSNGRLGSGIAPIRQLEEAGVPISIGVDGAASNEAADMVSETHAAWLMQRARRGQEATPS 499 SN RL SGIAPIR++ +AGVP+ +GVDG+ASN++A M+ E A L+QR Sbjct: 299 CSNMRLASGIAPIRKMLDAGVPVGLGVDGSASNDSAHMLGEVRQAMLLQRV--------- 349 Query: 500 YAGGSFEGGADAATVEDVVRWGTTGGARVLGLGALDGLQVGQQADIALYRLDDPRYFG-L 558 F G DA T + T GGA+VL + L+ G AD+ ++ + G L Sbjct: 350 ----GF--GPDAMTARQALEVATLGGAKVLNRDDIGALKPGMSADVVMFDTRQIAFAGAL 403 Query: 559 HDPALGPVVGGGRPYLKALYVAGRAVVEDDGIPGIDLAAL 598 HDP + +V + + GR +V + + I+L A+ Sbjct: 404 HDP-VAALVFCTPANVDCSIINGRVIVREGQLTSIELGAV 442 >gi|300313548|ref|YP_003777640.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 259 Score = 85.1 bits (209), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 47/168 (27%), Positives = 90/168 (53%) Query: 15 APFMIGVMLLLAWQLICTALAVPVYLVPTPSVIARTLAADWGLLSESLLVTLKITFMAFV 74 AP+ + +++L WQ IC+ V ++ P+P I + + G ++E L T +T + F Sbjct: 17 APWFLLLVILGVWQFICSVWQVSDFIFPSPGSILQAMVDYAGPITEHALSTFWVTMVGFA 76 Query: 75 LSVLLGVAIAFVFVQSRFIEVSLFPYAIILQVTPIVAIAPLIIIWVKEPTFALVICATIM 134 ++V++GV + FV S + + +P P A P++++W ++ A ++ Sbjct: 77 IAVVVGVTLGFVIGSSPMLYAAAYPLMTAFNALPKAAFVPVLVVWFGIGAGPAILTAFLI 136 Query: 135 ALFPIISNTTLGLRSVNPGLLNLFRLNKATRWQILRRLRIPSALPYFF 182 + FPI+ N GL ++ P L ++ R+ A R IL ++ +P ++PYFF Sbjct: 137 SFFPIMVNIATGLATLEPELEDVLRVLGARRMDILLKVGLPRSMPYFF 184 >gi|300309985|ref|YP_003774077.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 278 Score = 56.6 bits (135), Expect = 7e-09, Method: Compositional matrix adjust. Identities = 42/149 (28%), Positives = 70/149 (46%), Gaps = 2/149 (1%) Query: 36 VPVYLVPTPSVIARTL--AADWGLLSESLLVTLKITFMAFVLSVLLGVAIAFVFVQSRFI 93 +P L+P PS +A TL A G L + +L FV+ L VA+ + SR Sbjct: 48 IPARLLPPPSELAHTLWSLAQRGELLPHIAASLARVAAGFVIGSALAVALGLLVGISRRA 107 Query: 94 EVSLFPYAIILQVTPIVAIAPLIIIWVKEPTFALVICATIMALFPIISNTTLGLRSVNPG 153 E L P L+ P +A PL+++W+ V+ I A FP+ N G+R ++ Sbjct: 108 EALLEPSFQALRAIPSLAWIPLLLLWLGIDETPKVVLIAIGAFFPVYLNLVAGIRDIDHK 167 Query: 154 LLNLFRLNKATRWQILRRLRIPSALPYFF 182 L+ + + ++ + +P++LPY F Sbjct: 168 LIEVGTTSGLNTQALIGHILLPASLPYLF 196 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.140 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,175,143 Number of extensions: 98606 Number of successful extensions: 491 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 489 Number of HSP's successfully gapped: 13 Length of query: 662 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 571 Effective length of database: 1,188,985 Effective search space: 678910435 Effective search space used: 678910435 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 134 (56.2 bits)