BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0147.0011.p (216 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309800|ref|YP_003773892.1| isopenicillin N synthase [Herba... 276 1e-75 >gi|300309800|ref|YP_003773892.1| isopenicillin N synthase [Herbaspirillum seropedicae SmR1] Length = 328 Score = 276 bits (706), Expect = 1e-75, Method: Compositional matrix adjust. Identities = 135/204 (66%), Positives = 158/204 (77%), Gaps = 17/204 (8%) Query: 11 MIPYTPPKTVQRLPVIDFADAFSSDLGKREAVAWEIHKIARDVGFFYLVNHGVPSALVDG 70 MIPYTPP T +R+PVIDFADAFS +L +REA+AWEIHKI+RDVGFFYLVNHGVP AL+DG Sbjct: 1 MIPYTPPMTARRIPVIDFADAFSPELARREALAWEIHKISRDVGFFYLVNHGVPQALIDG 60 Query: 71 QFDWARRFFFFCRTGCRRARFFFFFAAPDAVKRAVDMSLSPAGFGYERIGTQALDDG-PT 129 QFDWARRFF A P A K A+DM S +G+GYER+G QALD+G P Sbjct: 61 QFDWARRFF----------------ALPQASKSAIDMRHSASGYGYERMGAQALDEGSPA 104 Query: 130 DLKEGFQFGTDLPPDHPYVRQGVLRYGHNLWPQELPGMRAHAQQYHAAMSVLSQRLLSII 189 DLKEGFQFG D+ PDHPYV +G+LRYGHNLWPQ+LPG H++QY+ A+ LS RLL +I Sbjct: 105 DLKEGFQFGFDIAPDHPYVLRGLLRYGHNLWPQDLPGFETHSRQYYEAVRALSHRLLGVI 164 Query: 190 ALSLQLPEDFFDFALGTPIATQRM 213 ALSL++PEDFF L TPIATQRM Sbjct: 165 ALSLEMPEDFFTPVLHTPIATQRM 188 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.142 0.454 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 695,134 Number of extensions: 27795 Number of successful extensions: 68 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 66 Number of HSP's successfully gapped: 1 Length of query: 216 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 135 Effective length of database: 1,236,335 Effective search space: 166905225 Effective search space used: 166905225 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 128 (53.9 bits)